Rorug05G0414200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001786
Physical Location & Seq
Reverse (-)
57263905 .. 57264751
847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0414200.1

Sequence Viewer

Length: 600 bp
ATGGCCTTGGCCAGTGACTACTGGAAAGTGGTGGGCGACACTTGCTGCTATACATATCTTGAGGGCATTAAAGAACCTGAAAACCAGTTGCTACGCACCATGTTCTTGATGGATTTGAAGTCCACAATCAGACGCCTCCACTCTGCTGATGTTCACGAGGAAGAAATCATAGCAGACAACGTCCGGATTAGCGAGAACCAGGACTCCATTGAACTCCAGTATAACCCCAATGAAAAGTTCCTTCGAGACTTGCAAGGTGTTGCTCTATCCAAGAAGCTTCAAGAGCTGCAAGTTCCGATTGAGGCTCGTAGCTCCTTGGTGGATACCATAATGTTCAAGGCCTCTGACACTGAATGGAAATATCAAGCTAGCCGCAAGATTTTGCGAATGAGGGTAGAGGTTGATCTCTATGTTATTGATGATGACACAGATGGTGACGATGGTACTATTGATGATTATGGTGTGGCTAGGTTTGTGCCGGCAAGTACACTGCCCATTGAGAAACTCGGGAGGACTAGAGCCGAAGTGCCATCGACAATGGGTTCGGAAGCTACACTCATGCCTTGTTCACATCTTTATAATGGGGGCTGCGTTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

199

Amino Acids

22.55

Weight (kDa)

4.7

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
AccIII TCCGGA 1 cut(s) 183
AciI CCGC 1 cut(s) 373
AcoI YGGCCR 1 cut(s) 9
AcyI GRCGYC 1 cut(s) 133
AfaI GTAC 2 cut(s) 445, 487
AgsI TTSAA 4 cut(s) 118, 212, 281, 337
AjnI CCWGG 1 cut(s) 198
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
AluBI AGCT 5 cut(s) 277, 286, 312, 368, 551
AluI AGCT 5 cut(s) 277, 286, 312, 368, 551
Alw26I GTCTC 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 183
AoxI GGCC 3 cut(s) 3, 9, 339
ApeKI GCWGC 3 cut(s) 45, 286, 588
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
AsuHPI GGTGA 1 cut(s) 446
AsuNHI GCTAGC 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 506
BalI TGGCCA 1 cut(s) 11
BauI CACGAG 1 cut(s) 155
BbvI GCAGC 3 cut(s) 32, 273, 575
BccI CCATC 4 cut(s) 103, 425, 434, 538
BciT130I CCWGG 1 cut(s) 200
BciVI GTATCC 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 3 cut(s) 369, 468, 516
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 4 cut(s) 46, 287, 373, 589
BlsI GCNGC 4 cut(s) 47, 288, 374, 590
Bme1390I CCNGG 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 506
BmrFI CCNGG 1 cut(s) 200
BmtI GCTAGC 1 cut(s) 372
BpmI CTGGAG 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 6, 315
BsaWI WCCGGW 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 4 cut(s) 12, 26, 85, 217
BseAI TCCGGA 1 cut(s) 183
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 2 cut(s) 6, 315
BseNI ACTGG 4 cut(s) 12, 26, 85, 217
BseXI GCAGC 3 cut(s) 32, 273, 575
BshFI GGCC 3 cut(s) 5, 11, 341
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 2 cut(s) 184, 479
BsmAI GTCTC 1 cut(s) 240
BsnI GGCC 3 cut(s) 5, 11, 341
BsoBI CYCGRG 1 cut(s) 506
Bsp13I TCCGGA 1 cut(s) 183
Bsp143I GATC 1 cut(s) 403
BspACI CCGC 1 cut(s) 373
BspANI GGCC 3 cut(s) 5, 11, 341
BspEI TCCGGA 1 cut(s) 183
BspOI GCTAGC 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 478
BsrI ACTGG 4 cut(s) 12, 26, 85, 217
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 6, 315
BssMI GATC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 133
BssSI CACGAG 1 cut(s) 155
BssT1I CCWWGG 2 cut(s) 6, 315
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 133
BstC8I GCNNGC 2 cut(s) 370, 480
BstKTI GATC 1 cut(s) 406
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 2 cut(s) 42, 283
BstNI CCWGG 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 198
BstV1I GCAGC 3 cut(s) 32, 273, 575
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 5, 11, 341
BtsI GCAGTG 1 cut(s) 488
BtsIMutI CAGTG 3 cut(s) 19, 348, 488
Cac8I GCNNGC 2 cut(s) 370, 480
Cfr10I RCCGGY 1 cut(s) 478
CseI GACGC 1 cut(s) 141
Csp6I GTAC 2 cut(s) 444, 486
CviAII CATG 2 cut(s) 100, 559
CviQI GTAC 2 cut(s) 444, 486
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 6, 315
Eco147I AGGCCT 1 cut(s) 341
Eco88I CYCGRG 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 6, 315
ErhI CCWWGG 2 cut(s) 6, 315
FaeI CATG 2 cut(s) 103, 562
FatI CATG 2 cut(s) 99, 558
Fnu4HI GCNGC 4 cut(s) 46, 287, 373, 589
Fsp4HI GCNGC 4 cut(s) 46, 287, 373, 589
FspBI CTAG 3 cut(s) 369, 468, 516
GluI GCNGC 4 cut(s) 46, 287, 373, 589
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 3 cut(s) 5, 11, 341
HapII CCGG 2 cut(s) 184, 479
HgaI GACGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 2 cut(s) 103, 562
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 1 cut(s) 203
HpaII CCGG 2 cut(s) 184, 479
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 4 cut(s) 123, 154, 488, 569
Hpy188I TCNGA 4 cut(s) 131, 297, 346, 547
Hpy188III TCNNGA 7 cut(s) 59, 106, 155, 184, 245, 281, 508
Hpy8I GTNNAC 4 cut(s) 123, 154, 488, 569
HpyAV CCTTC 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 2 cut(s) 253, 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 283
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 2 cut(s) 103, 562
Kpn2I TCCGGA 1 cut(s) 183
KroI GCCGGC 1 cut(s) 478
KroNI GCCGGC 1 cut(s) 480
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 1 cut(s) 317
LpnPI CCDG 9 cut(s) 7, 25, 90, 98, 185, 197, 212, 230, 492
Lsp1109I GCAGC 3 cut(s) 32, 273, 575
MaeI CTAG 3 cut(s) 369, 468, 516
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 14, 434
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 1 cut(s) 173
MlsI TGGCCA 1 cut(s) 11
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 1 cut(s) 197
MnlI CCTC 8 cut(s) 55, 146, 151, 295, 352, 384, 391, 504
Mox20I TGGCCA 1 cut(s) 11
MroI TCCGGA 1 cut(s) 183
MroNI GCCGGC 1 cut(s) 478
MscI TGGCCA 1 cut(s) 11
MseI TTAA 1 cut(s) 69
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 2 cut(s) 184, 479
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 2 cut(s) 42, 283
NaeI GCCGGC 1 cut(s) 480
NdeII GATC 1 cut(s) 403
NgoMIV GCCGGC 1 cut(s) 478
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 2 cut(s) 103, 562
NmuCI GTSAC 2 cut(s) 14, 434
PceI AGGCCT 1 cut(s) 341
PdiI GCCGGC 1 cut(s) 480
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 4 cut(s) 47, 288, 374, 590
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
PsiI TTATAA 1 cut(s) 579
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PsyI GACNNNGTC 1 cut(s) 179
RsaI GTAC 2 cut(s) 445, 487
RsaNI GTAC 2 cut(s) 444, 486
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 4 cut(s) 46, 287, 373, 589
Sau3AI GATC 1 cut(s) 403
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SseBI AGGCCT 1 cut(s) 341
SsiI CCGC 1 cut(s) 373
SspMI CTAG 3 cut(s) 369, 468, 516
StuI AGGCCT 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 2 cut(s) 6, 315
TaiI ACGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 244, 533
TatI WGTACW 1 cut(s) 485
TauI GCSGC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 3 cut(s) 19, 355, 495
TseFI GTSAC 2 cut(s) 14, 434
TseI GCWGC 3 cut(s) 45, 286, 588
Tsp45I GTSAC 2 cut(s) 14, 434
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 3 cut(s) 19, 355, 495
Tth111I GACNNNGTC 1 cut(s) 179
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XspI CTAG 3 cut(s) 369, 468, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.