MD11G1058600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001786
Physical Location & Seq
Reverse (-)
4986361 .. 4991195
4835 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1058600.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGCACCCACAACACTCTTTATTATTCTTCTCCTAGTGCACATTTCCAATTCCAACACTTGCGATGCCACAAATCCTGCACCAAAATTCCCAGCAGTTCTAGTGTTTGGCGATTCAACAGTCGACACTGGCAACAACAATTACCTCAAAACATTGTTCAAGGGCAACCATTACCCTTATGGCAAAGATTTCCCTGGCCAAATCCCCACAGGCAGATTCTCCAATGGAAAACTAGTTCCTGACTTTTTGGTCTCCATTTTAAACATCAAGCAAACAGTTCCTCCTTTTCTCGACCCGAGCCTTTCGGATAACGACCTCATCACCGGCGTCTGCTTCGCGTCAGGTGGATCCGGATACGATGATATAACCGCTGCTGGCGCCGGAATCCTAGCGTTTTCGAAGCAGATTGAGTTGTTCAAGGAATATGCAGTGAGAGTTGAGAGGATTTTAGGGGATAAGGAGGCAAAGAAGTTGATTACTAGTTCATTGGTTGTAGTAAGTGCAGGTACTAATGACTTTGGTTTTAATTTCTATGACATTCCCACAAGAAGATTAGAGTTCAACATTAGTGGATACCAAGATTTTCTGCTGAATAAGCTACACATATTTATTGAGGAACTGTATGAGCTTGGATGCAGAAAAATGGCAATAGCAGGGCTTCCTCCAGTTGGTTGCCTTCCCATACAAGTTACTGTAAAATTTGGGAAACCAAAAGATGGACGATGTGTAGATGATGAGAATTCAGATTCCCAAATATACAATCAAAAGCTTGTCAAACTATTGTCGAAAATGCAGTCACTGTTGCCAGGGAGCAGAATTGTTTATTTAGATGTCTATGAGCCGTTGATTGACATGATCAACAATCCACATAAATTTGGTTTTGTGGTAACGAAAAGAGGTTGCTGCGGTACTGGGTTTGTGGAAGCAGGGCCTTTGTGCAATGCACTGACTCCGGTATGCGCCGATGACTCGGAGTACTTGTTTTGGGATAGCATACATCCAACCGAAGCAACTTACCAGTACCTCTCAAAGTACCTAGAGAAGAATGTGCTTCCCAAGTTTGCTTATCGTAATCAATCGCGGCCGTCGATTACTCGGTCCGGCCATCCCTATCGGGCAGATGATCGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

41.84

Weight (kDa)

6.6

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 345 1.1e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 494
AccB1I GGYRCC 2 cut(s) 4, 377
AccB7I CCANNNNNTGG 1 cut(s) 716
AccI GTMKAC 1 cut(s) 123
AccII CGCG 2 cut(s) 338, 1081
AccIII TCCGGA 1 cut(s) 350
AciI CCGC 3 cut(s) 369, 906, 1081
AclWI GGATC 2 cut(s) 342, 355
AcoI YGGCCR 3 cut(s) 196, 1082, 1102
AcsI RAATTY 4 cut(s) 86, 698, 739, 872
AcyI GRCGYC 2 cut(s) 327, 378
AfaI GTAC 5 cut(s) 508, 910, 977, 1022, 1034
AfiI CCNNNNNNNGG 2 cut(s) 668, 716
AgsI TTSAA 4 cut(s) 117, 160, 418, 562
AhlI ACTAGT 2 cut(s) 232, 479
AjnI CCWGG 2 cut(s) 193, 805
AloI GAACNNNNNNTCC 2 cut(s) 219, 251
AluBI AGCT 3 cut(s) 598, 628, 769
AluI AGCT 3 cut(s) 598, 628, 769
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 256
Alw44I GTGCAC 1 cut(s) 38
AlwI GGATC 2 cut(s) 342, 355
AlwNI CAGNNNCTG 1 cut(s) 799
Ama87I CYCGRG 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 4 cut(s) 196, 929, 1082, 1102
ApaLI GTGCAC 1 cut(s) 38
ApeKI GCWGC 2 cut(s) 371, 903
ApoI RAATTY 4 cut(s) 86, 698, 739, 872
ArsI GACNNNNNNTTYG 2 cut(s) 779, 811
AspLEI GCGC 2 cut(s) 380, 962
AspS9I GGNCC 2 cut(s) 929, 1098
AsuHPI GGTGA 1 cut(s) 313
AsuII TTCGAA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 295
AvaII GGWCC 1 cut(s) 1098
BaeGI GKGCMC 1 cut(s) 42
BalI TGGCCA 1 cut(s) 198
BamHI GGATCC 1 cut(s) 347
BanI GGYRCC 2 cut(s) 4, 377
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 358, 890
BccI CCATC 2 cut(s) 710, 1113
BceAI ACGGC 2 cut(s) 826, 1069
BciT130I CCWGG 2 cut(s) 195, 807
BciVI GTATCC 2 cut(s) 347, 566
BclI TGATCA 1 cut(s) 855
BcoDI GTCTC 1 cut(s) 256
BcuI ACTAGT 2 cut(s) 232, 479
BfaI CTAG 6 cut(s) 35, 101, 233, 389, 480, 1037
BfoI RGCGCY 1 cut(s) 381
BfuAI ACCTGC 1 cut(s) 494
BfuI GTATCC 2 cut(s) 347, 566
BisI GCNGC 3 cut(s) 372, 904, 1082
BlsI GCNGC 3 cut(s) 373, 905, 1083
BmcAI AGTACT 1 cut(s) 977
Bme1390I CCNGG 2 cut(s) 195, 807
Bme18I GGWCC 1 cut(s) 1098
BmeT110I CYCGRG 1 cut(s) 295
BmgT120I GGNCC 2 cut(s) 929, 1098
BmiI GGNNCC 3 cut(s) 6, 349, 379
BmrFI CCNGG 2 cut(s) 195, 807
BmrI ACTGGG 1 cut(s) 921
BmsI GCATC 2 cut(s) 55, 623
BmuI ACTGGG 1 cut(s) 921
BpmI CTGGAG 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 1126
BsaHI GRCGYC 2 cut(s) 327, 378
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 193, 806
BsaWI WCCGGW 2 cut(s) 350, 952
BsaXI ACNNNNNCTCC 4 cut(s) 265, 295, 965, 995
Bsc4I CCNNNNNNNGG 2 cut(s) 668, 716
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 4 cut(s) 133, 665, 916, 1018
Bse3DI GCAATG 1 cut(s) 946
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 2 cut(s) 195, 807
BseCI ATCGAT 1 cut(s) 1126
BseDI CCNNGG 2 cut(s) 193, 806
BseGI GGATG 3 cut(s) 638, 997, 1105
BseLI CCNNNNNNNGG 2 cut(s) 668, 716
BseMI GCAATG 1 cut(s) 946
BseNI ACTGG 4 cut(s) 133, 665, 916, 1018
BseSI GKGCMC 1 cut(s) 42
BseX3I CGGCCG 1 cut(s) 1082
BseXI GCAGC 2 cut(s) 358, 890
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 2 cut(s) 63, 522
Bsh1236I CGCG 2 cut(s) 338, 1081
Bsh1285I CGRYCG 1 cut(s) 1085
BshFI GGCC 4 cut(s) 198, 931, 1084, 1104
BshNI GGYRCC 2 cut(s) 4, 377
BshVI ATCGAT 1 cut(s) 1126
BsiEI CGRYCG 1 cut(s) 1085
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 295
BsiSI CCGG 5 cut(s) 324, 351, 381, 953, 1101
BslI CCNNNNNNNGG 2 cut(s) 668, 716
BsmAI GTCTC 1 cut(s) 256
BsnI GGCC 4 cut(s) 198, 931, 1084, 1104
Bso31I GGTCTC 1 cut(s) 256
BsoBI CYCGRG 1 cut(s) 295
Bsp119I TTCGAA 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 42
Bsp13I TCCGGA 1 cut(s) 350
Bsp143I GATC 3 cut(s) 347, 855, 1123
BspACI CCGC 3 cut(s) 369, 906, 1081
BspANI GGCC 4 cut(s) 198, 931, 1084, 1104
BspDI ATCGAT 1 cut(s) 1126
BspEI TCCGGA 1 cut(s) 350
BspFNI CGCG 2 cut(s) 338, 1081
BspLI GGNNCC 3 cut(s) 6, 349, 379
BspMI ACCTGC 1 cut(s) 494
BspPI GGATC 2 cut(s) 342, 355
BspT104I TTCGAA 1 cut(s) 398
BspT107I GGYRCC 2 cut(s) 4, 377
BspTNI GGTCTC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 946
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 4 cut(s) 133, 665, 916, 1018
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 2 cut(s) 193, 806
BssMI GATC 3 cut(s) 347, 855, 1123
BssNI GRCGYC 2 cut(s) 327, 378
Bst2UI CCWGG 2 cut(s) 195, 807
Bst4CI ACNGT 5 cut(s) 121, 277, 621, 694, 801
BstACI GRCGYC 2 cut(s) 327, 378
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 376
BstF5I GGATG 3 cut(s) 638, 997, 1105
BstFNI CGCG 2 cut(s) 338, 1081
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 2 cut(s) 380, 962
BstKTI GATC 3 cut(s) 350, 858, 1126
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 3 cut(s) 347, 855, 1123
BstMCI CGRYCG 1 cut(s) 1085
BstMWI GCNNNNNNNGC 2 cut(s) 377, 595
BstNI CCWGG 2 cut(s) 195, 807
BstSCI CCNGG 2 cut(s) 193, 805
BstSLI GKGCMC 1 cut(s) 42
BstUI CGCG 2 cut(s) 338, 1081
BstV1I GCAGC 2 cut(s) 358, 890
BstX2I RGATCY 1 cut(s) 347
BstYI RGATCY 1 cut(s) 347
BstZI CGGCCG 1 cut(s) 1082
Bsu15I ATCGAT 1 cut(s) 1126
BsuI GTATCC 2 cut(s) 347, 566
BsuRI GGCC 4 cut(s) 198, 931, 1084, 1104
BsuTUI ATCGAT 1 cut(s) 1126
BtgZI GCGATG 1 cut(s) 78
BtsCI GGATG 3 cut(s) 638, 997, 1105
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 4 cut(s) 126, 435, 797, 944
BveI ACCTGC 1 cut(s) 494
Cac8I GCNNGC 1 cut(s) 376
CaiI CAGNNNCTG 1 cut(s) 799
CfoI GCGC 2 cut(s) 380, 962
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 2 cut(s) 929, 1098
ClaI ATCGAT 1 cut(s) 1126
CpoI CGGWCCG 1 cut(s) 1098
CseI GACGC 2 cut(s) 316, 327
Csp6I GTAC 5 cut(s) 507, 909, 976, 1021, 1033
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CspI CGGWCCG 1 cut(s) 1098
CviAII CATG 1 cut(s) 853
CviQI GTAC 5 cut(s) 507, 909, 976, 1021, 1033
DinI GGCGCC 1 cut(s) 379
DpnI GATC 3 cut(s) 349, 857, 1125
DpnII GATC 3 cut(s) 347, 855, 1123
DraI TTTAAA 1 cut(s) 261
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 3 cut(s) 196, 1082, 1102
EagI CGGCCG 1 cut(s) 1082
EclXI CGGCCG 1 cut(s) 1082
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 1 cut(s) 1098
Eco52I CGGCCG 1 cut(s) 1082
Eco88I CYCGRG 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 929
EcoRI GAATTC 1 cut(s) 739
EcoRII CCWGG 2 cut(s) 193, 805
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
FaeI CATG 1 cut(s) 856
FatI CATG 1 cut(s) 852
FbaI TGATCA 1 cut(s) 855
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 372, 904, 1082
FokI GGATG 3 cut(s) 645, 984, 1092
Fsp4HI GCNGC 3 cut(s) 372, 904, 1082
FspBI CTAG 6 cut(s) 35, 101, 233, 389, 480, 1037
GlaI GCGC 2 cut(s) 379, 961
GluI GCNGC 3 cut(s) 372, 904, 1082
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 1 cut(s) 648
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 4 cut(s) 198, 931, 1084, 1104
HapII CCGG 5 cut(s) 324, 351, 381, 953, 1101
HgaI GACGC 2 cut(s) 316, 327
HhaI GCGC 2 cut(s) 380, 962
Hin1I GRCGYC 2 cut(s) 327, 378
Hin1II CATG 1 cut(s) 856
Hin6I GCGC 2 cut(s) 378, 960
HinP1I GCGC 2 cut(s) 378, 960
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 6 cut(s) 113, 216, 384, 746, 949, 968
HpaII CCGG 5 cut(s) 324, 351, 381, 953, 1101
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 2 cut(s) 40, 124
Hpy188I TCNGA 3 cut(s) 307, 745, 973
Hpy188III TCNNGA 3 cut(s) 239, 290, 351
Hpy8I GTNNAC 2 cut(s) 40, 124
Hpy99I CGWCG 1 cut(s) 1090
HpyAV CCTTC 1 cut(s) 686
HpyCH4III ACNGT 5 cut(s) 121, 277, 621, 694, 801
HpyCH4V TGCA 8 cut(s) 40, 80, 428, 503, 636, 793, 939, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 595
Hsp92I GRCGYC 2 cut(s) 327, 378
Hsp92II CATG 1 cut(s) 856
HspAI GCGC 2 cut(s) 378, 960
KasI GGCGCC 1 cut(s) 377
Kpn2I TCCGGA 1 cut(s) 350
Ksp22I TGATCA 1 cut(s) 855
Kzo9I GATC 3 cut(s) 347, 855, 1123
LmnI GCTCC 1 cut(s) 810
Lsp1109I GCAGC 2 cut(s) 358, 890
LweI GCATC 2 cut(s) 55, 623
MaeI CTAG 6 cut(s) 35, 101, 233, 389, 480, 1037
MaeIII GTNAC 3 cut(s) 688, 795, 886
MalI GATC 3 cut(s) 349, 857, 1125
MboI GATC 3 cut(s) 347, 855, 1123
MboII GAAGA 3 cut(s) 20, 561, 1054
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 1 cut(s) 42
MlsI TGGCCA 1 cut(s) 198
MluCI AATT 8 cut(s) 49, 86, 139, 526, 698, 739, 816, 872
MluNI TGGCCA 1 cut(s) 198
Mly113I GGCGCC 1 cut(s) 378
MlyI GAGTC 2 cut(s) 943, 962
MmeI TCCRAC 2 cut(s) 78, 1025
MnlI CCTC 9 cut(s) 155, 291, 326, 435, 454, 607, 672, 890, 1034
Mox20I TGGCCA 1 cut(s) 198
MroI TCCGGA 1 cut(s) 350
MscI TGGCCA 1 cut(s) 198
MseI TTAA 2 cut(s) 260, 525
Msp20I TGGCCA 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 5 cut(s) 324, 351, 381, 953, 1101
MspR9I CCNGG 2 cut(s) 195, 807
MvaI CCWGG 2 cut(s) 195, 807
MvnI CGCG 2 cut(s) 338, 1081
MwoI GCNNNNNNNGC 2 cut(s) 377, 595
NarI GGCGCC 1 cut(s) 378
NdeII GATC 3 cut(s) 347, 855, 1123
NlaIII CATG 1 cut(s) 856
NlaIV GGNNCC 3 cut(s) 6, 349, 379
NmuCI GTSAC 1 cut(s) 795
NspV TTCGAA 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 1085
PfeI GAWTC 4 cut(s) 113, 216, 384, 746
PflMI CCANNNNNTGG 1 cut(s) 716
PkrI GCNGC 3 cut(s) 373, 905, 1083
PleI GAGTC 2 cut(s) 943, 962
PluTI GGCGCC 1 cut(s) 381
PpsI GAGTC 2 cut(s) 943, 962
Psp6I CCWGG 2 cut(s) 193, 805
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 2 cut(s) 193, 805
PspN4I GGNNCC 3 cut(s) 6, 349, 379
PspPI GGNCC 2 cut(s) 929, 1098
PstNI CAGNNNCTG 1 cut(s) 799
PsuI RGATCY 1 cut(s) 347
RsaI GTAC 5 cut(s) 508, 910, 977, 1022, 1034
RsaNI GTAC 5 cut(s) 507, 909, 976, 1021, 1033
Rsr2I CGGWCCG 1 cut(s) 1098
RsrII CGGWCCG 1 cut(s) 1098
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 2 cut(s) 260, 525
SatI GCNGC 3 cut(s) 372, 904, 1082
Sau3AI GATC 3 cut(s) 347, 855, 1123
Sau96I GGNCC 2 cut(s) 929, 1098
ScaI AGTACT 1 cut(s) 977
SchI GAGTC 2 cut(s) 943, 962
ScrFI CCNGG 2 cut(s) 195, 807
SduI GDGCHC 1 cut(s) 42
SfaNI GCATC 2 cut(s) 55, 623
SfoI GGCGCC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 398
SgrAI CRCCGGYG 1 cut(s) 323
SinI GGWCC 1 cut(s) 1098
SpeI ACTAGT 2 cut(s) 232, 479
Sse9I AATT 8 cut(s) 49, 86, 139, 526, 698, 739, 816, 872
SsiI CCGC 3 cut(s) 369, 906, 1081
SspDI GGCGCC 1 cut(s) 377
SspMI CTAG 6 cut(s) 35, 101, 233, 389, 480, 1037
StyD4I CCNGG 2 cut(s) 193, 805
TaaI ACNGT 5 cut(s) 121, 277, 621, 694, 801
TaqI TCGA 6 cut(s) 123, 291, 398, 785, 1088, 1126
TaqII GACCGA 1 cut(s) 1086
TasI AATT 8 cut(s) 49, 86, 139, 526, 698, 739, 816, 872
TatI WGTACW 1 cut(s) 975
TauI GCSGC 1 cut(s) 1084
TfiI GAWTC 4 cut(s) 113, 216, 384, 746
Tru1I TTAA 2 cut(s) 260, 525
Tru9I TTAA 2 cut(s) 260, 525
TscAI CASTG 4 cut(s) 133, 435, 804, 951
TseFI GTSAC 1 cut(s) 795
TseI GCWGC 2 cut(s) 371, 903
Tsp45I GTSAC 1 cut(s) 795
TspDTI ATGAA 1 cut(s) 474
TspRI CASTG 4 cut(s) 133, 435, 804, 951
Van91I CCANNNNNTGG 1 cut(s) 716
VneI GTGCAC 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 1098
XapI RAATTY 4 cut(s) 86, 698, 739, 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 6 cut(s) 35, 101, 233, 389, 480, 1037
ZrmI AGTACT 1 cut(s) 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.