AT2G30310

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001786
Physical Location & Seq
Forward (+)
12923055 .. 12924380
1326 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30310.1

Sequence Viewer

Length: 1080 bp
ATGTCTACATCTAAAACCATAGTGTTTGGTCTCTTTGTCGCAACACTCCTCGTCTCTTGCAATGTGGCTGCAAACGCCACAACGCAGCCGCTTTTCCCAGCGATTCTAATCTTCGGTGACTCAACCGTAGACACAGGCAACAACAACTACCATTCACAAACCATCTTCAAGGCTAAGCATCTCCCTTACGGCGTTGATCTACCGGGCCATGAAGCTAACGGAAGATATTCAAATGGAAAAGTAATCTCTGACGTCATTGCTTCCAAACTCAACATCAAAGAGTTGGTTCCTCCGTTTCTACAACCAAACATCTCCCACCAAGACATTGTCACTGGCGTCTCTTTCGCATCCGCGGGTGCGGGCTACGATGACCGGAGCTCACTCTCGAGCAAAGCAATCCCGGTCTCACAACAGCCAAGCATGTTTAAGAATTATATTGCTCGTCTCAAAGGTATCGTAGGAGACAAGAAAGCAATGGAGATCATTAACAACGCTTTAGTGGTCATAAGCGCAGGGCCTAACGATTTCATCTTGAACTTTTATGATATCCCCACGAGGCGTCTTGAGTATCCTACTATCCATGGTTACCAAGAGTTTATCCTCAAGAGGCTTGACGGTTTTGTCCGGGAGCTTTATAGTTTAGGTTGCCGAAATATTGTGGTCGGAGGGTTACCACCAATGGGATGTTTACCAATCCAAATGACCGCGAAAATGAGAAACATTTTGAGATTTTGTGTGGAACAAGAAAACAAAGACTCCGTTTTGTACAATCAGAAACTAGTAAAGAAACTGCCTGAGATCCAAGCGTCTCTACCGGGAAGCAATTTCCTTTACGCCAACGTCTACGACCCATTGATGGACATGATCCAAAACCCTAGTAAATACGGATTCAAGGAGACTAAGAAAGGATGTTGCGGAACAGGATACTTGGAAACAACATTCATGTGCAATCCCCTTACAAAGACTTGTCCGAATCACTCCGATCACTTGTTTTGGGACTCAATTCATCCCTCGGAAGCTGCCTACAATTACATTGGCAACTTTGTGGATGCTCAGATTCGAGGCTGGATTAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

359

Amino Acids

39.74

Weight (kDa)

8.64

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 347 8.7e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 620
AatII GACGTC 1 cut(s) 255
AccI GTMKAC 3 cut(s) 5, 129, 843
AccII CGCG 2 cut(s) 353, 707
AciI CCGC 6 cut(s) 89, 351, 353, 359, 705, 915
AclWI GGATC 2 cut(s) 793, 859
AcyI GRCGYC 3 cut(s) 252, 336, 559
AfaI GTAC 1 cut(s) 767
AfiI CCNNNNNNNGG 2 cut(s) 680, 856
AgsI TTSAA 4 cut(s) 169, 231, 535, 892
AhlI ACTAGT 1 cut(s) 778
AluBI AGCT 4 cut(s) 215, 378, 631, 1019
AluI AGCT 4 cut(s) 215, 378, 631, 1019
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 8 cut(s) 35, 58, 343, 409, 449, 456, 813, 890
AlwI GGATC 2 cut(s) 793, 859
Ama87I CYCGRG 1 cut(s) 385
AoxI GGCC 2 cut(s) 205, 515
ApeKI GCWGC 3 cut(s) 68, 85, 1019
Asp700I GAANNNNTTC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 512
AspS9I GGNCC 2 cut(s) 205, 515
AsuC2I CCSGG 4 cut(s) 204, 401, 626, 816
AsuHPI GGTGA 1 cut(s) 128
AvaI CYCGRG 1 cut(s) 385
BanII GRGCYC 1 cut(s) 380
BauI CACGAG 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 3 cut(s) 55, 97, 1006
BccI CCATC 2 cut(s) 170, 850
BceAI ACGGC 1 cut(s) 205
BciVI GTATCC 2 cut(s) 579, 917
BcnI CCSGG 4 cut(s) 204, 401, 626, 816
BcoDI GTCTC 8 cut(s) 35, 58, 343, 409, 449, 456, 813, 890
BcuI ACTAGT 1 cut(s) 778
BfaI CTAG 2 cut(s) 779, 876
BfuI GTATCC 2 cut(s) 579, 917
BisI GCNGC 4 cut(s) 69, 86, 89, 1020
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 4 cut(s) 70, 87, 90, 1021
Bme1390I CCNGG 4 cut(s) 204, 401, 626, 816
BmeT110I CYCGRG 1 cut(s) 385
BmgT120I GGNCC 2 cut(s) 205, 515
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 4 cut(s) 204, 401, 626, 816
BmsI GCATC 3 cut(s) 187, 356, 1039
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 2 cut(s) 584, 587
BpuMI CCSGG 4 cut(s) 204, 401, 626, 816
BsaBI GATNNNNATC 1 cut(s) 107
BsaHI GRCGYC 3 cut(s) 252, 336, 559
BsaI GGTCTC 2 cut(s) 35, 409
BsaJI CCNNGG 3 cut(s) 351, 580, 1011
BsaWI WCCGGW 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 2 cut(s) 680, 856
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 3 cut(s) 67, 255, 480
Bse8I GATNNNNATC 1 cut(s) 107
BseDI CCNNGG 3 cut(s) 351, 580, 1011
BseGI GGATG 5 cut(s) 347, 689, 914, 1006, 1054
BseJI GATNNNNATC 1 cut(s) 107
BseLI CCNNNNNNNGG 2 cut(s) 680, 856
BseMI GCAATG 3 cut(s) 67, 255, 480
BseMII CTCAG 2 cut(s) 786, 1067
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 3 cut(s) 55, 97, 1006
BseYI CCCAGC 1 cut(s) 97
Bsh1236I CGCG 2 cut(s) 353, 707
BshFI GGCC 2 cut(s) 207, 517
BsiHKAI GWGCWC 1 cut(s) 380
BsiHKCI CYCGRG 1 cut(s) 385
BsiSI CCGG 5 cut(s) 203, 373, 401, 625, 815
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 2 cut(s) 680, 856
BsmAI GTCTC 8 cut(s) 35, 58, 343, 409, 449, 456, 813, 890
BsmBI CGTCTC 4 cut(s) 58, 343, 449, 813
BsmFI GGGAC 1 cut(s) 1010
BsnI GGCC 2 cut(s) 207, 517
Bso31I GGTCTC 2 cut(s) 35, 409
BsoBI CYCGRG 1 cut(s) 385
Bsp1286I GDGCHC 1 cut(s) 380
Bsp1407I TGTACA 1 cut(s) 765
Bsp143I GATC 5 cut(s) 196, 480, 798, 864, 982
Bsp1720I GCTNAGC 1 cut(s) 174
Bsp19I CCATGG 1 cut(s) 580
BspACI CCGC 6 cut(s) 89, 351, 353, 359, 705, 915
BspANI GGCC 2 cut(s) 207, 517
BspCNI CTCAG 2 cut(s) 787, 1066
BspFNI CGCG 2 cut(s) 353, 707
BspLI GGNNCC 1 cut(s) 288
BspPI GGATC 2 cut(s) 793, 859
BspTNI GGTCTC 2 cut(s) 35, 409
BsrDI GCAATG 3 cut(s) 67, 255, 480
BsrGI TGTACA 1 cut(s) 765
BsrI ACTGG 1 cut(s) 337
BssECI CCNNGG 3 cut(s) 351, 580, 1011
BssMI GATC 5 cut(s) 196, 480, 798, 864, 982
BssNI GRCGYC 3 cut(s) 252, 336, 559
BssSI CACGAG 1 cut(s) 553
BssT1I CCWWGG 1 cut(s) 580
Bst2BI CACGAG 1 cut(s) 553
Bst4CI ACNGT 2 cut(s) 127, 617
BstACI GRCGYC 3 cut(s) 252, 336, 559
BstAUI TGTACA 1 cut(s) 765
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 4 cut(s) 174, 795, 900, 1053
BstDSI CCRYGG 2 cut(s) 351, 580
BstEII GGTNACC 2 cut(s) 584, 669
BstF5I GGATG 5 cut(s) 347, 689, 914, 1006, 1054
BstFNI CGCG 2 cut(s) 353, 707
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 5 cut(s) 199, 483, 801, 867, 985
BstMAI GTCTC 8 cut(s) 35, 58, 343, 409, 449, 456, 813, 890
BstMBI GATC 5 cut(s) 196, 480, 798, 864, 982
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNSI RCATGY 1 cut(s) 424
BstPI GGTNACC 2 cut(s) 584, 669
BstSCI CCNGG 4 cut(s) 202, 399, 624, 814
BstUI CGCG 2 cut(s) 353, 707
BstV1I GCAGC 3 cut(s) 55, 97, 1006
BstX2I RGATCY 1 cut(s) 798
BstYI RGATCY 1 cut(s) 798
BsuI GTATCC 2 cut(s) 579, 917
BsuRI GGCC 2 cut(s) 207, 517
BtgI CCRYGG 2 cut(s) 351, 580
BtsCI GGATG 5 cut(s) 347, 689, 914, 1006, 1054
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 361
CfoI GCGC 1 cut(s) 512
Cfr13I GGNCC 2 cut(s) 205, 515
Cfr42I CCGCGG 1 cut(s) 354
CseI GACGC 3 cut(s) 325, 548, 795
Csp6I GTAC 1 cut(s) 766
CviAII CATG 5 cut(s) 209, 421, 581, 862, 943
CviQI GTAC 1 cut(s) 766
DdeI CTNAG 4 cut(s) 174, 795, 900, 1053
DpnI GATC 5 cut(s) 198, 482, 800, 866, 984
DpnII GATC 5 cut(s) 196, 480, 798, 864, 982
DrdI GACNNNNNNGTC 1 cut(s) 620
DseDI GACNNNNNNGTC 1 cut(s) 620
Ecl136II GAGCTC 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 380
Eco31I GGTCTC 2 cut(s) 35, 409
Eco32I GATATC 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 385
Eco91I GGTNACC 2 cut(s) 584, 669
EcoICRI GAGCTC 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 515
EcoO65I GGTNACC 2 cut(s) 584, 669
EcoRV GATATC 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 580
EcoT38I GRGCYC 1 cut(s) 380
ErhI CCWWGG 1 cut(s) 580
Esp3I CGTCTC 4 cut(s) 58, 343, 449, 813
FaeI CATG 5 cut(s) 212, 424, 584, 865, 946
FaqI GGGAC 1 cut(s) 1010
FatI CATG 5 cut(s) 208, 420, 580, 861, 942
FauI CCCGC 2 cut(s) 346, 352
FblI GTMKAC 3 cut(s) 5, 129, 843
Fnu4HI GCNGC 4 cut(s) 69, 86, 89, 1020
FokI GGATG 5 cut(s) 334, 696, 921, 993, 1061
FriOI GRGCYC 1 cut(s) 380
Fsp4HI GCNGC 4 cut(s) 69, 86, 89, 1020
FspBI CTAG 2 cut(s) 779, 876
GlaI GCGC 1 cut(s) 511
GluI GCNGC 4 cut(s) 69, 86, 89, 1020
GsaI CCCAGC 1 cut(s) 101
HaeIII GGCC 2 cut(s) 207, 517
HapII CCGG 5 cut(s) 203, 373, 401, 625, 815
HgaI GACGC 3 cut(s) 325, 548, 795
HhaI GCGC 1 cut(s) 512
Hin1I GRCGYC 3 cut(s) 252, 336, 559
Hin1II CATG 5 cut(s) 212, 424, 584, 865, 946
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HinfI GANTC 7 cut(s) 103, 119, 755, 888, 973, 998, 1057
HpaII CCGG 5 cut(s) 203, 373, 401, 625, 815
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 4 cut(s) 6, 130, 689, 844
Hpy188I TCNGA 7 cut(s) 250, 665, 774, 972, 982, 1015, 1056
Hpy188III TCNNGA 4 cut(s) 385, 532, 563, 604
Hpy8I GTNNAC 4 cut(s) 6, 130, 689, 844
HpyCH4III ACNGT 2 cut(s) 127, 617
HpyCH4IV ACGT 2 cut(s) 252, 840
HpyCH4V TGCA 3 cut(s) 60, 71, 948
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 4 cut(s) 174, 795, 900, 1053
HpySE526I ACGT 2 cut(s) 252, 840
Hsp92I GRCGYC 3 cut(s) 252, 336, 559
Hsp92II CATG 5 cut(s) 212, 424, 584, 865, 946
HspAI GCGC 1 cut(s) 510
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 5 cut(s) 196, 480, 798, 864, 982
LmnI GCTCC 2 cut(s) 375, 628
Lsp1109I GCAGC 3 cut(s) 55, 97, 1006
LweI GCATC 3 cut(s) 187, 356, 1039
MaeI CTAG 2 cut(s) 779, 876
MaeII ACGT 2 cut(s) 252, 840
MaeIII GTNAC 4 cut(s) 116, 328, 584, 669
MalI GATC 5 cut(s) 198, 482, 800, 866, 984
MboI GATC 5 cut(s) 196, 480, 798, 864, 982
MboII GAAGA 3 cut(s) 103, 157, 234
MflI RGATCY 1 cut(s) 798
MhlI GDGCHC 1 cut(s) 380
MluCI AATT 4 cut(s) 430, 823, 1002, 1027
MlyI GAGTC 3 cut(s) 113, 749, 992
MmeI TCCRAC 1 cut(s) 643
MnlI CCTC 8 cut(s) 59, 300, 549, 600, 611, 659, 1021, 1055
MroXI GAANNNNTTC 1 cut(s) 226
MseI TTAA 4 cut(s) 426, 486, 1071, 1078
MslI CAYNNNNRTG 1 cut(s) 943
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 5 cut(s) 203, 373, 401, 625, 815
MspR9I CCNGG 4 cut(s) 204, 401, 626, 816
MvnI CGCG 2 cut(s) 353, 707
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 4 cut(s) 204, 401, 626, 816
NcoI CCATGG 1 cut(s) 580
NdeII GATC 5 cut(s) 196, 480, 798, 864, 982
NlaIII CATG 5 cut(s) 212, 424, 584, 865, 946
NlaIV GGNNCC 1 cut(s) 288
NmuCI GTSAC 2 cut(s) 116, 328
NspI RCATGY 1 cut(s) 424
PaeR7I CTCGAG 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 226
PfeI GAWTC 4 cut(s) 103, 888, 973, 1057
PflFI GACNNNGTC 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 624
PkrI GCNGC 4 cut(s) 70, 87, 90, 1021
PleI GAGTC 3 cut(s) 113, 749, 992
PpsI GAGTC 3 cut(s) 113, 749, 992
Psp124BI GAGCTC 1 cut(s) 380
PspEI GGTNACC 2 cut(s) 584, 669
PspFI CCCAGC 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 288
PspPI GGNCC 2 cut(s) 205, 515
PsuI RGATCY 1 cut(s) 798
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 1 cut(s) 767
RsaNI GTAC 1 cut(s) 766
RseI CAYNNNNRTG 1 cut(s) 943
SacI GAGCTC 1 cut(s) 380
SacII CCGCGG 1 cut(s) 354
SaqAI TTAA 4 cut(s) 426, 486, 1071, 1078
SatI GCNGC 4 cut(s) 69, 86, 89, 1020
Sau3AI GATC 5 cut(s) 196, 480, 798, 864, 982
Sau96I GGNCC 2 cut(s) 205, 515
SchI GAGTC 3 cut(s) 113, 749, 992
ScrFI CCNGG 4 cut(s) 204, 401, 626, 816
SduI GDGCHC 1 cut(s) 380
SetI ASST 8 cut(s) 217, 255, 380, 454, 633, 646, 843, 1021
SfaNI GCATC 3 cut(s) 187, 356, 1039
Sfr274I CTCGAG 1 cut(s) 385
Sfr303I CCGCGG 1 cut(s) 354
SgrBI CCGCGG 1 cut(s) 354
SlaI CTCGAG 1 cut(s) 385
SmiMI CAYNNNNRTG 1 cut(s) 943
SmlI CTYRAG 3 cut(s) 385, 563, 602
SmoI CTYRAG 3 cut(s) 385, 563, 602
SpeI ACTAGT 1 cut(s) 778
Sse9I AATT 4 cut(s) 430, 823, 1002, 1027
SsiI CCGC 6 cut(s) 89, 351, 353, 359, 705, 915
SspI AATATT 1 cut(s) 655
SspMI CTAG 2 cut(s) 779, 876
SstI GAGCTC 1 cut(s) 380
StyD4I CCNGG 4 cut(s) 202, 399, 624, 814
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 2 cut(s) 127, 617
TaiI ACGT 2 cut(s) 255, 843
TaqI TCGA 2 cut(s) 386, 1060
TasI AATT 4 cut(s) 430, 823, 1002, 1027
TatI WGTACW 1 cut(s) 765
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 4 cut(s) 103, 888, 973, 1057
Tru1I TTAA 4 cut(s) 426, 486, 1071, 1078
Tru9I TTAA 4 cut(s) 426, 486, 1071, 1078
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 2 cut(s) 116, 328
TseI GCWGC 3 cut(s) 68, 85, 1019
Tsp45I GTSAC 2 cut(s) 116, 328
TspDTI ATGAA 4 cut(s) 225, 517, 931, 995
TspGWI ACGGA 4 cut(s) 234, 282, 748, 900
TspRI CASTG 1 cut(s) 337
Tth111I GACNNNGTC 1 cut(s) 326
XceI RCATGY 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 385
XmiI GTMKAC 3 cut(s) 5, 129, 843
XmnI GAANNNNTTC 1 cut(s) 226
XspI CTAG 2 cut(s) 779, 876
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.