AT2G31550

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001786
Physical Location & Seq
Reverse (-)
13433797 .. 13435207
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31550.1

Sequence Viewer

Length: 660 bp
ATGTTCAAGAGTTACATTGCTCGTCTCAAAGGTATCGTGGGAGACAAGAAAGCGATGGAGATCATAAACAATGCTTTCGTGGTTGTGAGTGCAGGGCCTAATGATTTCATTTTGAACTATTACGATATTCCCTCAAGGCGTCTCGAGTATCCTTTCATTTCTGGTTACCAAGATTTTATTCTTAAGAGGCTTGAAAATTTCGTGCGGGAGCTTTACAGTTTAGGTGTCCGGAATGTTTTGGTTGGAGGTTTACCGCCAATGGGGTGTTTACCGATCCATATGACTGCTAAATTCCGCAACATTTTTAGATTCTGCTTGGAACACCATAACAAAGACTCTGTTTTGTACAATGAGAAACTTCAGAAGCTCTTGCCCCAAATCGAAGCATCTCTTCCAGGAAGCAAATTCCTTTACGCCGATGTCTATAATCCTATGATGGAGATGATCCAAAACCCTAGCAAATACGGGTTCAAAGAGACGAAGAGAGGATGTTGTGGAACAGGGTTCTTGGAGACGAGCTTCATGTGTAATGTCTTTTCTCCTGTGTGTCAAAATCGGTCGGAGTTTATGTTCTTTGACTCGATTCATCCATCGGAAGCTACCTATAATGTCATAGGCAATCGTCTGGATCCTCTGATTCGTGGGAAGTTTCAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

219

Amino Acids

25.16

Weight (kDa)

8.97

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 8 - 209 2.2e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 3 cut(s) 205, 254, 295
AclWI GGATC 4 cut(s) 268, 439, 623, 636
AcsI RAATTY 3 cut(s) 196, 290, 404
AcuI CTGAAG 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 1 cut(s) 260
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 4 cut(s) 7, 115, 194, 472
AjnI CCWGG 1 cut(s) 394
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AloI GAACNNNNNNTCC 2 cut(s) 554, 586
AluBI AGCT 4 cut(s) 211, 367, 519, 599
AluI AGCT 4 cut(s) 211, 367, 519, 599
Alw26I GTCTC 5 cut(s) 29, 36, 146, 470, 506
AlwI GGATC 4 cut(s) 268, 439, 623, 636
Ama87I CYCGRG 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 1 cut(s) 95
ApoI RAATTY 3 cut(s) 196, 290, 404
AspS9I GGNCC 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 143
BamHI GGATCC 1 cut(s) 628
BccI CCATC 3 cut(s) 49, 430, 598
BciT130I CCWGG 1 cut(s) 396
BciVI GTATCC 1 cut(s) 159
BcoDI GTCTC 5 cut(s) 29, 36, 146, 470, 506
BfaI CTAG 1 cut(s) 456
BfrI CTTAAG 1 cut(s) 182
BfuI GTATCC 1 cut(s) 159
Bme1390I CCNGG 1 cut(s) 396
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 630
BmrFI CCNGG 1 cut(s) 396
BmsI GCATC 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 139
BsaWI WCCGGW 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse3DI GCAATG 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 59
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 1 cut(s) 396
BseGI GGATG 2 cut(s) 494, 586
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 111
Bsh1285I CGRYCG 1 cut(s) 560
BshFI GGCC 1 cut(s) 97
BsiEI CGRYCG 1 cut(s) 560
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 5 cut(s) 29, 36, 146, 470, 506
BsmBI CGTCTC 4 cut(s) 29, 146, 470, 506
BsnI GGCC 1 cut(s) 97
BsoBI CYCGRG 1 cut(s) 143
Bsp13I TCCGGA 1 cut(s) 228
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 4 cut(s) 60, 273, 444, 628
BspACI CCGC 3 cut(s) 205, 254, 295
BspANI GGCC 1 cut(s) 97
BspEI TCCGGA 1 cut(s) 228
BspLI GGNNCC 1 cut(s) 630
BspPI GGATC 4 cut(s) 268, 439, 623, 636
BspTI CTTAAG 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 15
BsrGI TGTACA 1 cut(s) 345
BssMI GATC 4 cut(s) 60, 273, 444, 628
BssNI GRCGYC 1 cut(s) 139
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 1 cut(s) 218
Bst6I CTCTTC 2 cut(s) 396, 476
BstACI GRCGYC 1 cut(s) 139
BstAFI CTTAAG 1 cut(s) 182
BstAUI TGTACA 1 cut(s) 345
BstEII GGTNACC 1 cut(s) 164
BstF5I GGATG 2 cut(s) 494, 586
BstKTI GATC 4 cut(s) 63, 276, 447, 631
BstMAI GTCTC 5 cut(s) 29, 36, 146, 470, 506
BstMBI GATC 4 cut(s) 60, 273, 444, 628
BstMCI CGRYCG 1 cut(s) 560
BstNI CCWGG 1 cut(s) 396
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 628
BstYI RGATCY 1 cut(s) 628
BsuI GTATCC 1 cut(s) 159
BsuRI GGCC 1 cut(s) 97
BtgZI GCGATG 1 cut(s) 68
BtsCI GGATG 2 cut(s) 494, 586
Cfr13I GGNCC 1 cut(s) 95
CseI GACGC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 346
CviAII CATG 1 cut(s) 523
CviJI RGCY 7 cut(s) 97, 190, 211, 367, 519, 599, 656
CviKI_1 RGCY 7 cut(s) 97, 190, 211, 367, 519, 599, 656
CviQI GTAC 1 cut(s) 346
DpnI GATC 4 cut(s) 62, 275, 446, 630
DpnII GATC 4 cut(s) 60, 273, 444, 628
Eam1104I CTCTTC 2 cut(s) 396, 476
EarI CTCTTC 2 cut(s) 396, 476
Eco57I CTGAAG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 143
Eco91I GGTNACC 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 95
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 394
Esp3I CGTCTC 4 cut(s) 29, 146, 470, 506
FaeI CATG 1 cut(s) 526
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FatI CATG 1 cut(s) 522
FauI CCCGC 1 cut(s) 198
FauNDI CATATG 1 cut(s) 279
FokI GGATG 2 cut(s) 501, 573
FspBI CTAG 1 cut(s) 456
HaeIII GGCC 1 cut(s) 97
HapII CCGG 1 cut(s) 229
HgaI GACGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 1 cut(s) 526
HinfI GANTC 5 cut(s) 309, 335, 578, 583, 637
HpaII CCGG 1 cut(s) 229
Hpy166II GTNNAC 2 cut(s) 251, 269
Hpy188I TCNGA 4 cut(s) 363, 562, 595, 636
Hpy188III TCNNGA 4 cut(s) 7, 143, 229, 626
Hpy8I GTNNAC 2 cut(s) 251, 269
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4V TGCA 1 cut(s) 92
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 1 cut(s) 526
Kpn2I TCCGGA 1 cut(s) 228
Kzo9I GATC 4 cut(s) 60, 273, 444, 628
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 9 cut(s) 78, 147, 242, 381, 408, 486, 555, 611, 638
LweI GCATC 1 cut(s) 395
MaeI CTAG 1 cut(s) 456
MaeIII GTNAC 2 cut(s) 11, 164
MalI GATC 4 cut(s) 62, 275, 446, 630
MboI GATC 4 cut(s) 60, 273, 444, 628
MboII GAAGA 2 cut(s) 383, 493
MflI RGATCY 1 cut(s) 628
MluCI AATT 3 cut(s) 196, 290, 404
MlyI GAGTC 2 cut(s) 329, 572
MmeI TCCRAC 2 cut(s) 223, 540
MnlI CCTC 5 cut(s) 142, 180, 239, 479, 642
MroI TCCGGA 1 cut(s) 228
MseI TTAA 2 cut(s) 183, 658
MspCI CTTAAG 1 cut(s) 182
MspI CCGG 1 cut(s) 229
MspR9I CCNGG 1 cut(s) 396
MvaI CCWGG 1 cut(s) 396
NdeI CATATG 1 cut(s) 279
NdeII GATC 4 cut(s) 60, 273, 444, 628
NlaIII CATG 1 cut(s) 526
NlaIV GGNNCC 1 cut(s) 630
PaeR7I CTCGAG 1 cut(s) 143
PfeI GAWTC 3 cut(s) 309, 583, 637
PfoI TCCNGGA 1 cut(s) 394
PleI GAGTC 2 cut(s) 329, 572
PpsI GAGTC 2 cut(s) 329, 572
Psp6I CCWGG 1 cut(s) 394
PspEI GGTNACC 1 cut(s) 164
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 1 cut(s) 630
PspPI GGNCC 1 cut(s) 95
PsuI RGATCY 1 cut(s) 628
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
SaqAI TTAA 2 cut(s) 183, 658
Sau3AI GATC 4 cut(s) 60, 273, 444, 628
Sau96I GGNCC 1 cut(s) 95
SchI GAGTC 2 cut(s) 329, 572
ScrFI CCNGG 1 cut(s) 396
SetI ASST 8 cut(s) 34, 213, 226, 250, 369, 521, 601, 605
SfaNI GCATC 1 cut(s) 395
Sfr274I CTCGAG 1 cut(s) 143
SlaI CTCGAG 1 cut(s) 143
SmlI CTYRAG 3 cut(s) 133, 143, 182
SmoI CTYRAG 3 cut(s) 133, 143, 182
Sse9I AATT 3 cut(s) 196, 290, 404
SsiI CCGC 3 cut(s) 205, 254, 295
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 1 cut(s) 394
TaaI ACNGT 1 cut(s) 218
TaqI TCGA 3 cut(s) 144, 381, 581
TaqII GACCGA 1 cut(s) 546
TasI AATT 3 cut(s) 196, 290, 404
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 3 cut(s) 309, 583, 637
Tru1I TTAA 2 cut(s) 183, 658
Tru9I TTAA 2 cut(s) 183, 658
TspDTI ATGAA 4 cut(s) 97, 145, 511, 575
Vha464I CTTAAG 1 cut(s) 182
XapI RAATTY 3 cut(s) 196, 290, 404
XhoI CTCGAG 1 cut(s) 143
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.