Rh5DG500600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001786
Physical Location & Seq
Reverse (-)
78469199 .. 78471302
2104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG500600.1

Sequence Viewer

Length: 1137 bp
ATGGCACCCACAGGCACAACCCTCTTTCTTATACTAGTCCATATTTGGTTTTGCACCTTATTATTCATTTCCAACACATGCAAAGCCACAAGGCCTACACCAAAATTCCCAGCCATTCTAGTCTTTGGGGATTCAACAGTGGACACAGGCAACAACAATTACCTCAAAACATTGTTTAAGGGTAACCATTATCCATATGGCCAAGATTTTCCAGGTCATGTTACTACAGGCAGGTTTTCTAATGGAAAATTGGTTCCCGACTTCCTTGCCTCCATGTTAAACATCAAAGAAACTGTTCCTCCTTTTCTTGATCCGAGCCTCTCGGATAACAACCTTGTCACCGGTGTCAGCTTTGCTTCGGGTGGATCTGGATTCGACGATATTACAGGTAGTGTAGGTGGGATCATTCCATTTTCAAAGCAGGTTGAGTACTTCAAGAGATACATAGTGAGAGTCCAAGGTATTGTAGGGGAAAAGGAGGCACAGAAGTTAATTAGTAGTGCAGTGGTTGTGATCAGTGGTGGGACTAATGACTTTGGTTTTAATTTCTATGACATCCCCACAAGAAGGTTAGAGTTCAATATTAGTGGCTACCAAGATTTTCTGCAGAATAAACTGCATATATTCATAGAGGAACTTTATGAGCTTGGATGCAGAAGAATGGCTATAGCTGGGCTTCCTCCAATAGGTTGCCTACCCATACAAATAACTGTAAAATTTGAAAGTCCACATGATAGAAGATGTATAGAGGAAGAAAATTTGGATGCACTTGTATACAATAAGAAGCTTGCAAGGCTCTTGCCCAAAATACAATCACTGCTGCCAAGGAGCAAAATTGTGTATGCAGACGTGTATATGCCTTTTATTGACATGATCAATAATCCACTAAACTTTGGTTTTCTGGAAACAAAAAGAGGCTGCTGTGGTACTGGGTTAGTGGAAGTAGGACCCTCCTGTAATTCATTAACAGCAGTATGTGATAATGTCTTGGAGTACTTGTTTTGGGATAGCATACATCCAAGTCAAGCAGCATATCTCTACATTTCAAAATACCTGGAGAAGAAAATCATTTCCGAGCTTCTTGATTACGATAATCAATCACAGCCGTTAGTTACTCTATTTGATCAGAAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

42.2

Weight (kDa)

6.1

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 351 3e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 222, 412
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 774
AclWI GGATC 3 cut(s) 305, 373, 410
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 3 cut(s) 104, 716, 757
AfaI GTAC 3 cut(s) 431, 928, 995
AfiI CCNNNNNNNGG 2 cut(s) 567, 686
AflIII ACRYGT 1 cut(s) 849
AgeI ACCGGT 1 cut(s) 341
AgsI TTSAA 6 cut(s) 135, 417, 436, 580, 722, 1047
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 2 cut(s) 211, 1053
AloI GAACNNNNNNTCC 2 cut(s) 237, 269
AluBI AGCT 5 cut(s) 351, 646, 671, 787, 1078
AluI AGCT 5 cut(s) 351, 646, 671, 787, 1078
AlwI GGATC 3 cut(s) 305, 373, 410
AoxI GGCC 2 cut(s) 92, 199
ApeKI GCWGC 3 cut(s) 820, 918, 1028
ApoI RAATTY 3 cut(s) 104, 716, 757
AsiGI ACCGGT 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 947
AsuHPI GGTGA 1 cut(s) 331
AvaII GGWCC 1 cut(s) 947
BalI TGGCCA 1 cut(s) 201
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 3 cut(s) 807, 905, 1040
BceAI ACGGC 1 cut(s) 1090
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 2 cut(s) 213, 1055
BclI TGATCA 3 cut(s) 513, 873, 1123
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 2 cut(s) 35, 119
BfmI CTRYAG 3 cut(s) 225, 605, 666
BfuAI ACCTGC 2 cut(s) 222, 412
BisI GCNGC 3 cut(s) 821, 919, 1029
BlsI GCNGC 3 cut(s) 822, 920, 1030
BmcAI AGTACT 2 cut(s) 431, 995
Bme1390I CCNGG 2 cut(s) 213, 1055
Bme18I GGWCC 1 cut(s) 947
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 1 cut(s) 947
BmiI GGNNCC 3 cut(s) 6, 255, 949
BmrFI CCNGG 2 cut(s) 213, 1055
BmrI ACTGGG 1 cut(s) 939
BmsI GCATC 2 cut(s) 641, 754
BmuI ACTGGG 1 cut(s) 939
BpmI CTGGAG 1 cut(s) 1076
BsaJI CCNNGG 2 cut(s) 457, 824
BsaWI WCCGGW 1 cut(s) 341
BsaXI ACNNNNNCTCC 4 cut(s) 283, 313, 983, 1013
Bsc4I CCNNNNNNNGG 2 cut(s) 567, 686
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 934
BseBI CCWGG 2 cut(s) 213, 1055
BseDI CCNNGG 2 cut(s) 457, 824
BseGI GGATG 4 cut(s) 555, 656, 769, 1015
BseLI CCNNNNNNNGG 2 cut(s) 567, 686
BseNI ACTGG 1 cut(s) 934
BseXI GCAGC 3 cut(s) 807, 905, 1040
BseYI CCCAGC 2 cut(s) 109, 671
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 2 cut(s) 94, 201
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 341
BsiSI CCGG 1 cut(s) 342
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 2 cut(s) 567, 686
BsmFI GGGAC 1 cut(s) 538
BsnI GGCC 2 cut(s) 94, 201
Bsp143I GATC 6 cut(s) 310, 365, 402, 513, 873, 1123
BspANI GGCC 2 cut(s) 94, 201
BspLI GGNNCC 3 cut(s) 6, 255, 949
BspMAI CTGCAG 1 cut(s) 609
BspMI ACCTGC 2 cut(s) 222, 412
BspPI GGATC 3 cut(s) 305, 373, 410
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 934
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 457, 824
BssMI GATC 6 cut(s) 310, 365, 402, 513, 873, 1123
BssNAI GTATAC 1 cut(s) 775
BssT1I CCWWGG 2 cut(s) 457, 824
Bst1107I GTATAC 1 cut(s) 775
Bst2UI CCWGG 2 cut(s) 213, 1055
Bst4CI ACNGT 3 cut(s) 139, 295, 712
BstC8I GCNNGC 1 cut(s) 789
BstEII GGTNACC 1 cut(s) 182
BstENI CCTNNNNNAGG 1 cut(s) 684
BstF5I GGATG 4 cut(s) 555, 656, 769, 1015
BstKTI GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
BstMBI GATC 6 cut(s) 310, 365, 402, 513, 873, 1123
BstMWI GCNNNNNNNGC 1 cut(s) 793
BstNI CCWGG 2 cut(s) 213, 1055
BstNSI RCATGY 1 cut(s) 81
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 211, 1053
BstSFI CTRYAG 3 cut(s) 225, 605, 666
BstV1I GCAGC 3 cut(s) 807, 905, 1040
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BstZ17I GTATAC 1 cut(s) 775
BsuRI GGCC 2 cut(s) 94, 201
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 4 cut(s) 555, 656, 769, 1015
BtsI GCAGTG 2 cut(s) 510, 815
BtsIMutI CAGTG 4 cut(s) 144, 510, 523, 815
BveI ACCTGC 2 cut(s) 222, 412
Cac8I GCNNGC 1 cut(s) 789
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 947
Csp6I GTAC 3 cut(s) 430, 927, 994
CspAI ACCGGT 1 cut(s) 341
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 5 cut(s) 78, 218, 274, 731, 871
CviQI GTAC 3 cut(s) 430, 927, 994
DpnI GATC 6 cut(s) 312, 367, 404, 515, 875, 1125
DpnII GATC 6 cut(s) 310, 365, 402, 513, 873, 1123
EaeI YGGCCR 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 457, 824
Eco147I AGGCCT 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 947
Eco91I GGTNACC 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 684
EcoO109I RGGNCCY 1 cut(s) 947
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 211, 1053
EcoT14I CCWWGG 2 cut(s) 457, 824
ErhI CCWWGG 2 cut(s) 457, 824
FaeI CATG 5 cut(s) 81, 221, 277, 734, 874
FaqI GGGAC 1 cut(s) 538
FatI CATG 5 cut(s) 77, 217, 273, 730, 870
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 3 cut(s) 513, 873, 1123
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 3 cut(s) 821, 919, 1029
FokI GGATG 4 cut(s) 542, 663, 776, 1002
Fsp4HI GCNGC 3 cut(s) 821, 919, 1029
FspBI CTAG 2 cut(s) 35, 119
GluI GCNGC 3 cut(s) 821, 919, 1029
GsaI CCCAGC 2 cut(s) 113, 675
GsuI CTGGAG 1 cut(s) 1076
HaeIII GGCC 2 cut(s) 94, 201
HapII CCGG 1 cut(s) 342
Hin1II CATG 5 cut(s) 81, 221, 277, 734, 874
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 3 cut(s) 131, 372, 453
HpaII CCGG 1 cut(s) 342
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 3 cut(s) 142, 728, 775
Hpy188I TCNGA 4 cut(s) 315, 325, 1075, 1128
Hpy188III TCNNGA 6 cut(s) 257, 308, 369, 436, 902, 1082
Hpy8I GTNNAC 3 cut(s) 142, 728, 775
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 1 cut(s) 561
HpyCH4III ACNGT 3 cut(s) 139, 295, 712
HpyCH4IV ACGT 1 cut(s) 849
HpyCH4V TGCA 9 cut(s) 54, 81, 503, 607, 619, 654, 767, 791, 845
HpyF10VI GCNNNNNNNGC 1 cut(s) 793
HpySE526I ACGT 1 cut(s) 849
Hsp92II CATG 5 cut(s) 81, 221, 277, 734, 874
Ksp22I TGATCA 3 cut(s) 513, 873, 1123
Kzo9I GATC 6 cut(s) 310, 365, 402, 513, 873, 1123
LmnI GCTCC 1 cut(s) 828
Lsp1109I GCAGC 3 cut(s) 807, 905, 1040
LweI GCATC 2 cut(s) 641, 754
MaeI CTAG 2 cut(s) 35, 119
MaeII ACGT 1 cut(s) 849
MaeIII GTNAC 4 cut(s) 182, 220, 337, 1111
MalI GATC 6 cut(s) 312, 367, 404, 515, 875, 1125
MboI GATC 6 cut(s) 310, 365, 402, 513, 873, 1123
MboII GAAGA 4 cut(s) 669, 750, 764, 1072
MflI RGATCY 1 cut(s) 365
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 9 cut(s) 104, 157, 248, 492, 544, 716, 757, 834, 958
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 1 cut(s) 462
MmeI TCCRAC 1 cut(s) 96
Mox20I TGGCCA 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 294
MscI TGGCCA 1 cut(s) 201
MseI TTAA 5 cut(s) 177, 278, 491, 543, 965
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 2 cut(s) 213, 1055
MvaI CCWGG 2 cut(s) 213, 1055
MwoI GCNNNNNNNGC 1 cut(s) 793
NdeI CATATG 1 cut(s) 196
NdeII GATC 6 cut(s) 310, 365, 402, 513, 873, 1123
NlaIII CATG 5 cut(s) 81, 221, 277, 734, 874
NlaIV GGNNCC 3 cut(s) 6, 255, 949
NmuCI GTSAC 1 cut(s) 337
NspI RCATGY 1 cut(s) 81
PceI AGGCCT 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 2 cut(s) 131, 372
PinAI ACCGGT 1 cut(s) 341
PkrI GCNGC 3 cut(s) 822, 920, 1030
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
PpuMI RGGWCCY 1 cut(s) 947
Psp5II RGGWCCY 1 cut(s) 947
Psp6I CCWGG 2 cut(s) 211, 1053
PspEI GGTNACC 1 cut(s) 182
PspFI CCCAGC 2 cut(s) 109, 671
PspGI CCWGG 2 cut(s) 211, 1053
PspN4I GGNNCC 3 cut(s) 6, 255, 949
PspPI GGNCC 1 cut(s) 947
PspPPI RGGWCCY 1 cut(s) 947
PstI CTGCAG 1 cut(s) 609
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 3 cut(s) 431, 928, 995
RsaNI GTAC 3 cut(s) 430, 927, 994
SaqAI TTAA 5 cut(s) 177, 278, 491, 543, 965
SatI GCNGC 3 cut(s) 821, 919, 1029
Sau3AI GATC 6 cut(s) 310, 365, 402, 513, 873, 1123
Sau96I GGNCC 1 cut(s) 947
ScaI AGTACT 2 cut(s) 431, 995
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 2 cut(s) 213, 1055
SfaNI GCATC 2 cut(s) 641, 754
SfcI CTRYAG 3 cut(s) 225, 605, 666
SgrAI CRCCGGYG 1 cut(s) 341
SinI GGWCC 1 cut(s) 947
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 9 cut(s) 104, 157, 248, 492, 544, 716, 757, 834, 958
SseBI AGGCCT 1 cut(s) 94
SspI AATATT 1 cut(s) 583
SspMI CTAG 2 cut(s) 35, 119
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 2 cut(s) 211, 1053
StyI CCWWGG 2 cut(s) 457, 824
TaaI ACNGT 3 cut(s) 139, 295, 712
TaiI ACGT 1 cut(s) 852
TaqI TCGA 1 cut(s) 375
TasI AATT 9 cut(s) 104, 157, 248, 492, 544, 716, 757, 834, 958
TatI WGTACW 2 cut(s) 429, 993
TfiI GAWTC 2 cut(s) 131, 372
Tru1I TTAA 5 cut(s) 177, 278, 491, 543, 965
Tru9I TTAA 5 cut(s) 177, 278, 491, 543, 965
TscAI CASTG 4 cut(s) 144, 510, 523, 822
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 820, 918, 1028
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 3 cut(s) 55, 616, 951
TspRI CASTG 4 cut(s) 144, 510, 523, 822
VpaK11BI GGWCC 1 cut(s) 947
XagI CCTNNNNNAGG 1 cut(s) 684
XapI RAATTY 3 cut(s) 104, 716, 757
XceI RCATGY 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 2 cut(s) 35, 119
ZrmI AGTACT 2 cut(s) 431, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.