FvH4_3g39810

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001786
Physical Location & Seq
Reverse (-)
33729095 .. 33731182
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39810.t1

Sequence Viewer

Length: 1104 bp
ATGGCACCCATAGGTACAACCCTCTTTCTTATACTAGTCCACATTTGGTTTTACGCCTTATTATTCATTTCGAACGAATGCAAAGCCGTAAGGCCTGCACCAAAGTTCCCGGCCATTCTAGTCTTTGGGGATTCAACTGTGGACACAGGCAACAACAATTACCTCAACACATCGTTTAAGGGTAACCATTATCCATATGGCCAAGATTTTCCAGGTCATGTTACCACAGGCAGGTTTTCTAATGGAAAATTGGTCCCTGACTTCCTTGCTTCCATGTTAAACATCAAAGAAACTGTTCCTCCTTTTCTTGATCCGAGCCTATCGGATAACGACCTTGTCACCGGTGTCAGCTTTGCTTCGGGTGGATCCGGGTTCGACGATATAACAGGTGCTGTAGGTGGGATCATACCATTTTCAAAGCAGGTTGAGTACTTCAAGAGATACATAGTGAGAGTCCAAGGGATTGTAGGGGAAAAGGAAGCAAAGAAGTTGATTAGTAGTGCAGTGATTGTAATCTGTGCTGGGACTAATGACTTTGGTTTTAATTTCTATGACCTCCCCACAAGAAGGTTGGAGTTCAATATTAGCGGCTACCAAGATTTTCTGCAGAATAAACTGCATAAGTTCATTGAGGAACTATATGAGCTTGGATGCAGAAGAATGACCATAGCTGGGCTTCCTCCAGTAGGTTGCCTACCTATACAAATGACTGCAAAATTTGAAAGGCCGCACGATAGAAGATGTATAGAGGAAGAAAATTTAGACGCCCTTGTATACAATAAGAAGCTTGCAAGACTCTTGCCCACAATACAATCACTGCTGCCAAGGAGCAAAATTGTGTATGCAGACGTGTATGAACCTATAACCGATATGATCGATAATCCACAAAACTTTGGTTTTGTGGAAACAAAGAGAGGCTGCTGTGGTACTGGGTCAGTGGAAGCAGGGCCCTTCTGTAATGCATTAACAGCAGTATGTGATAATGCCTTGGAGTACTTGTTTTGGGATGGCATACATCCAAGTGAAGCAGCATATGAATACATTTCAAAATACCTGGAGAAAAACGTTGTTCCCCAGCTTCTGGATTACAACAATAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

368

Amino Acids

40.93

Weight (kDa)

5.61

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 351 2.8e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 222, 412
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 1081
AccI GTMKAC 1 cut(s) 774
AciI CCGC 2 cut(s) 588, 728
AclI AACGTT 1 cut(s) 1065
AclWI GGATC 4 cut(s) 305, 360, 373, 410
AcoI YGGCCR 2 cut(s) 111, 199
AcsI RAATTY 2 cut(s) 716, 757
AcyI GRCGYC 1 cut(s) 765
AfaI GTAC 4 cut(s) 16, 431, 928, 995
AfiI CCNNNNNNNGG 5 cut(s) 231, 567, 672, 686, 1081
AflIII ACRYGT 1 cut(s) 849
AgeI ACCGGT 1 cut(s) 341
AgsI TTSAA 6 cut(s) 135, 417, 436, 580, 722, 1047
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 2 cut(s) 211, 1053
AluBI AGCT 5 cut(s) 351, 646, 671, 787, 1078
AluI AGCT 5 cut(s) 351, 646, 671, 787, 1078
AlwI GGATC 4 cut(s) 305, 360, 373, 410
AlwNI CAGNNNCTG 2 cut(s) 392, 1081
AoxI GGCC 5 cut(s) 92, 111, 199, 725, 947
ApaI GGGCCC 1 cut(s) 951
ApeKI GCWGC 3 cut(s) 820, 918, 1028
ApoI RAATTY 2 cut(s) 716, 757
AsiGI ACCGGT 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 294
AspS9I GGNCC 3 cut(s) 253, 947, 948
AsuC2I CCSGG 2 cut(s) 110, 370
AsuHPI GGTGA 1 cut(s) 331
AsuII TTCGAA 1 cut(s) 71
AvaII GGWCC 1 cut(s) 253
BaeGI GKGCMC 1 cut(s) 951
BalI TGGCCA 1 cut(s) 201
BamHI GGATCC 1 cut(s) 365
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 951
BbvI GCAGC 3 cut(s) 807, 905, 1040
BccI CCATC 1 cut(s) 1001
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 2 cut(s) 213, 1055
BcnI CCSGG 2 cut(s) 110, 370
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 2 cut(s) 35, 119
BfmI CTRYAG 2 cut(s) 393, 605
BfuAI ACCTGC 2 cut(s) 222, 412
BisI GCNGC 5 cut(s) 589, 728, 821, 919, 1029
BlsI GCNGC 5 cut(s) 590, 729, 822, 920, 1030
BmcAI AGTACT 2 cut(s) 431, 995
Bme1390I CCNGG 4 cut(s) 110, 213, 370, 1055
Bme18I GGWCC 1 cut(s) 253
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 3 cut(s) 253, 947, 948
BmiI GGNNCC 4 cut(s) 6, 255, 367, 949
BmrFI CCNGG 4 cut(s) 110, 213, 370, 1055
BmrI ACTGGG 1 cut(s) 939
BmsI GCATC 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 939
BpmI CTGGAG 2 cut(s) 666, 1076
Bpu14I TTCGAA 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 110, 370
Bsa29I ATCGAT 1 cut(s) 876
BsaBI GATNNNNATC 1 cut(s) 512
BsaHI GRCGYC 1 cut(s) 765
BsaJI CCNNGG 3 cut(s) 457, 824, 987
BsaWI WCCGGW 1 cut(s) 341
BsaXI ACNNNNNCTCC 4 cut(s) 283, 313, 983, 1013
Bsc4I CCNNNNNNNGG 5 cut(s) 231, 567, 672, 686, 1081
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 2 cut(s) 683, 934
Bse8I GATNNNNATC 1 cut(s) 512
BseBI CCWGG 2 cut(s) 213, 1055
BseCI ATCGAT 1 cut(s) 876
BseDI CCNNGG 3 cut(s) 457, 824, 987
BseGI GGATG 3 cut(s) 656, 1012, 1015
BseJI GATNNNNATC 1 cut(s) 512
BseLI CCNNNNNNNGG 5 cut(s) 231, 567, 672, 686, 1081
BseNI ACTGG 2 cut(s) 683, 934
BseSI GKGCMC 1 cut(s) 951
BseXI GCAGC 3 cut(s) 807, 905, 1040
BseYI CCCAGC 3 cut(s) 521, 671, 1074
BsgI GTGCAG 2 cut(s) 81, 522
BshFI GGCC 5 cut(s) 94, 113, 201, 727, 949
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 341
BshVI ATCGAT 1 cut(s) 876
BsiSI CCGG 3 cut(s) 110, 342, 369
BslFI GGGAC 2 cut(s) 239, 538
BslI CCNNNNNNNGG 5 cut(s) 231, 567, 672, 686, 1081
BsmFI GGGAC 2 cut(s) 239, 538
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 5 cut(s) 94, 113, 201, 727, 949
Bsp119I TTCGAA 1 cut(s) 71
Bsp120I GGGCCC 1 cut(s) 947
Bsp1286I GDGCHC 1 cut(s) 951
Bsp143I GATC 4 cut(s) 310, 365, 402, 873
BspACI CCGC 2 cut(s) 588, 728
BspANI GGCC 5 cut(s) 94, 113, 201, 727, 949
BspDI ATCGAT 1 cut(s) 876
BspLI GGNNCC 4 cut(s) 6, 255, 367, 949
BspMAI CTGCAG 1 cut(s) 609
BspMI ACCTGC 2 cut(s) 222, 412
BspPI GGATC 4 cut(s) 305, 360, 373, 410
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 2 cut(s) 683, 934
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 3 cut(s) 457, 824, 987
BssMI GATC 4 cut(s) 310, 365, 402, 873
BssNAI GTATAC 1 cut(s) 775
BssNI GRCGYC 1 cut(s) 765
BssT1I CCWWGG 3 cut(s) 457, 824, 987
Bst1107I GTATAC 1 cut(s) 775
Bst2UI CCWGG 2 cut(s) 213, 1055
Bst4CI ACNGT 2 cut(s) 139, 295
BstACI GRCGYC 1 cut(s) 765
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 96, 789
BstEII GGTNACC 1 cut(s) 182
BstENI CCTNNNNNAGG 1 cut(s) 684
BstF5I GGATG 3 cut(s) 656, 1012, 1015
BstKTI GATC 4 cut(s) 313, 368, 405, 876
BstMBI GATC 4 cut(s) 310, 365, 402, 873
BstMWI GCNNNNNNNGC 1 cut(s) 968
BstNI CCWGG 2 cut(s) 213, 1055
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 4 cut(s) 108, 211, 368, 1053
BstSFI CTRYAG 2 cut(s) 393, 605
BstSLI GKGCMC 1 cut(s) 951
BstV1I GCAGC 3 cut(s) 807, 905, 1040
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BstZ17I GTATAC 1 cut(s) 775
Bsu15I ATCGAT 1 cut(s) 876
BsuRI GGCC 5 cut(s) 94, 113, 201, 727, 949
BsuTUI ATCGAT 1 cut(s) 876
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 3 cut(s) 656, 1012, 1015
BtsI GCAGTG 2 cut(s) 510, 815
BtsIMutI CAGTG 3 cut(s) 510, 815, 942
BveI ACCTGC 2 cut(s) 222, 412
Cac8I GCNNGC 2 cut(s) 96, 789
CaiI CAGNNNCTG 2 cut(s) 392, 1081
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 253, 947, 948
ClaI ATCGAT 1 cut(s) 876
CseI GACGC 1 cut(s) 773
Csp6I GTAC 4 cut(s) 15, 430, 927, 994
CspAI ACCGGT 1 cut(s) 341
CviAII CATG 2 cut(s) 218, 274
CviQI GTAC 4 cut(s) 15, 430, 927, 994
DpnI GATC 4 cut(s) 312, 367, 404, 875
DpnII GATC 4 cut(s) 310, 365, 402, 873
EaeI YGGCCR 2 cut(s) 111, 199
Eco130I CCWWGG 3 cut(s) 457, 824, 987
Eco147I AGGCCT 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 951
Eco47I GGWCC 1 cut(s) 253
Eco91I GGTNACC 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 684
EcoO109I RGGNCCY 2 cut(s) 947, 948
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 211, 1053
EcoT14I CCWWGG 3 cut(s) 457, 824, 987
EcoT22I ATGCAT 1 cut(s) 964
EcoT38I GRGCYC 1 cut(s) 951
ErhI CCWWGG 3 cut(s) 457, 824, 987
FaeI CATG 2 cut(s) 221, 277
FaqI GGGAC 2 cut(s) 239, 538
FatI CATG 2 cut(s) 217, 273
FauNDI CATATG 2 cut(s) 196, 1033
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 5 cut(s) 589, 728, 821, 919, 1029
FokI GGATG 3 cut(s) 663, 1002, 1019
FriOI GRGCYC 1 cut(s) 951
Fsp4HI GCNGC 5 cut(s) 589, 728, 821, 919, 1029
FspBI CTAG 2 cut(s) 35, 119
GluI GCNGC 5 cut(s) 589, 728, 821, 919, 1029
GsaI CCCAGC 3 cut(s) 525, 675, 1078
GsuI CTGGAG 2 cut(s) 666, 1076
HaeIII GGCC 5 cut(s) 94, 113, 201, 727, 949
HapII CCGG 3 cut(s) 110, 342, 369
HgaI GACGC 1 cut(s) 773
Hin1I GRCGYC 1 cut(s) 765
Hin1II CATG 2 cut(s) 221, 277
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 3 cut(s) 131, 453, 795
HpaII CCGG 3 cut(s) 110, 342, 369
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 3 cut(s) 40, 142, 775
Hpy188I TCNGA 2 cut(s) 315, 325
Hpy188III TCNNGA 4 cut(s) 308, 436, 1082, 1101
Hpy8I GTNNAC 3 cut(s) 40, 142, 775
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 2 cut(s) 561, 961
HpyCH4III ACNGT 2 cut(s) 139, 295
HpyCH4IV ACGT 2 cut(s) 849, 1065
HpyF10VI GCNNNNNNNGC 1 cut(s) 968
HpySE526I ACGT 2 cut(s) 849, 1065
Hsp92I GRCGYC 1 cut(s) 765
Hsp92II CATG 2 cut(s) 221, 277
Kzo9I GATC 4 cut(s) 310, 365, 402, 873
LmnI GCTCC 1 cut(s) 828
Lsp1109I GCAGC 3 cut(s) 807, 905, 1040
LweI GCATC 1 cut(s) 641
MaeI CTAG 2 cut(s) 35, 119
MaeII ACGT 2 cut(s) 849, 1065
MaeIII GTNAC 3 cut(s) 182, 220, 337
MalI GATC 4 cut(s) 312, 367, 404, 875
MboI GATC 4 cut(s) 310, 365, 402, 873
MboII GAAGA 3 cut(s) 669, 750, 764
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 951
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 6 cut(s) 157, 248, 544, 716, 757, 834
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 2 cut(s) 462, 789
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 8 cut(s) 32, 173, 309, 566, 625, 690, 742, 908
Mox20I TGGCCA 1 cut(s) 201
Mph1103I ATGCAT 1 cut(s) 964
MroXI GAANNNNTTC 1 cut(s) 294
MscI TGGCCA 1 cut(s) 201
MseI TTAA 4 cut(s) 177, 278, 543, 965
MslI CAYNNNNRTG 1 cut(s) 1020
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 3 cut(s) 110, 342, 369
MspR9I CCNGG 4 cut(s) 110, 213, 370, 1055
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 2 cut(s) 213, 1055
MwoI GCNNNNNNNGC 1 cut(s) 968
NciI CCSGG 2 cut(s) 110, 370
NdeI CATATG 2 cut(s) 196, 1033
NdeII GATC 4 cut(s) 310, 365, 402, 873
NlaIII CATG 2 cut(s) 221, 277
NlaIV GGNNCC 4 cut(s) 6, 255, 367, 949
NmuCI GTSAC 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 964
NspV TTCGAA 1 cut(s) 71
PceI AGGCCT 1 cut(s) 94
PctI GAATGC 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 131
PflFI GACNNNGTC 1 cut(s) 335
PflMI CCANNNNNTGG 1 cut(s) 1081
PinAI ACCGGT 1 cut(s) 341
PkrI GCNGC 5 cut(s) 590, 729, 822, 920, 1030
PleI GAGTC 2 cut(s) 461, 789
PpsI GAGTC 2 cut(s) 461, 789
Psp1406I AACGTT 1 cut(s) 1065
Psp6I CCWGG 2 cut(s) 211, 1053
PspEI GGTNACC 1 cut(s) 182
PspFI CCCAGC 3 cut(s) 521, 671, 1074
PspGI CCWGG 2 cut(s) 211, 1053
PspN4I GGNNCC 4 cut(s) 6, 255, 367, 949
PspOMI GGGCCC 1 cut(s) 947
PspPI GGNCC 3 cut(s) 253, 947, 948
PstI CTGCAG 1 cut(s) 609
PstNI CAGNNNCTG 2 cut(s) 392, 1081
PsuI RGATCY 1 cut(s) 365
PsyI GACNNNGTC 1 cut(s) 335
RsaI GTAC 4 cut(s) 16, 431, 928, 995
RsaNI GTAC 4 cut(s) 15, 430, 927, 994
RseI CAYNNNNRTG 1 cut(s) 1020
SaqAI TTAA 4 cut(s) 177, 278, 543, 965
SatI GCNGC 5 cut(s) 589, 728, 821, 919, 1029
Sau3AI GATC 4 cut(s) 310, 365, 402, 873
Sau96I GGNCC 3 cut(s) 253, 947, 948
ScaI AGTACT 2 cut(s) 431, 995
SchI GAGTC 2 cut(s) 462, 789
ScrFI CCNGG 4 cut(s) 110, 213, 370, 1055
SduI GDGCHC 1 cut(s) 951
SfaNI GCATC 1 cut(s) 641
SfcI CTRYAG 2 cut(s) 393, 605
SfuI TTCGAA 1 cut(s) 71
SgrAI CRCCGGYG 1 cut(s) 341
SinI GGWCC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 1020
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 6 cut(s) 157, 248, 544, 716, 757, 834
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 2 cut(s) 588, 728
SspI AATATT 1 cut(s) 583
SspMI CTAG 2 cut(s) 35, 119
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 4 cut(s) 108, 211, 368, 1053
StyI CCWWGG 3 cut(s) 457, 824, 987
TaaI ACNGT 2 cut(s) 139, 295
TaiI ACGT 2 cut(s) 852, 1068
TaqI TCGA 3 cut(s) 71, 375, 876
TasI AATT 6 cut(s) 157, 248, 544, 716, 757, 834
TatI WGTACW 2 cut(s) 429, 993
TauI GCSGC 2 cut(s) 591, 730
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 4 cut(s) 177, 278, 543, 965
Tru9I TTAA 4 cut(s) 177, 278, 543, 965
TscAI CASTG 3 cut(s) 510, 822, 942
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 820, 918, 1028
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 4 cut(s) 55, 616, 870, 1050
TspRI CASTG 3 cut(s) 510, 822, 942
Tth111I GACNNNGTC 1 cut(s) 335
Van91I CCANNNNNTGG 1 cut(s) 1081
VpaK11BI GGWCC 1 cut(s) 253
XagI CCTNNNNNAGG 1 cut(s) 684
XapI RAATTY 2 cut(s) 716, 757
XcmI CCANNNNNNNNNTGG 2 cut(s) 194, 568
XmiI GTMKAC 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 2 cut(s) 35, 119
ZrmI AGTACT 2 cut(s) 431, 995
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.