Rroxscaffold_4G00286280

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001786
Physical Location & Seq
Reverse (-)
7360528 .. 7365027
4500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286280.1

Sequence Viewer

Length: 1437 bp
ATGCAGGCTCTGTACAGTAGGGGAGCACGGCGGTTCATGGTGGTAGGTCTTCCACCAATCGGATGCCTTCCGCTTCAACGGACACTGAGCGGCATCATCAATCTGCGCCCTCAGCGCCTGTGCGTCACGCAGCAAAACATAGACTGTCAGACCTACAACTCCAAGCTCCAGGCCCTTCTCTCAAGTTTGCAAACCACACTACCACTCCCTAGTTCAAGGCTAGTCTATTTTGACATCTACAACCCCATCATGGACATGTACAACAACCCAGATAAATATGGGTTTGTGCATATACATGAAGGATGCTGTGGAACTGGGATGCTTGAGTTGGGGCCTCTGTGCACAGTGCTTTCGCGGACGTGCCCCGACGCGTCGAAGTATCTTTTCTGGGATGCAGTTCATCCTACTGAAGCAGCATTTTTCGTCATTGCTGACTATGCTCATAAGACTGTCTTGCAGTACCTAACTCTCCTTTATCGGTCTGAAGCCTCCGGCGAAACGTCACCGGTGTCCGAATTCATCTCAATGGTGGACAAAGTCTCGGAGTTTAGTCAGGATCAGGAAGAAAGGTTTTTGTACTATGTTTCTGTTGCAGAGGAGTTGGCGCAAGAGTTGAGTTCTTCAAATGGGAAATTGAAATCACTTATCGATGACTTAAGAAATGAAGTGGATTCTATCAGGACTTTCAGGAATGAACATTCTATGGAATACCAGAAGCTTCTGATGGAAGAGGAGCAGATGGCAGAAGAATTGAAGCTGTGTCTGGAAATTGGAAAGGACAGGTTCCAATCACAAGCTTATGAACCAACTTTTTTTGGGTATGCAATTATATGTTTATGTACCGACTTTCAGAGGTCCTCCTTTGATGAAAAATGCATAGAGTCCATTAAGCTTCTGATGGATGAGAAGCAGAAGGCAGAGGAGTCTTTGAAAATTGAAAAGGAGATGACGCGATGCCAGGCTAATGAACTGGCTTCCCTTAGATCTGCCTTAAATGAAAAGAGCACCGAGTGGCAGGAGCTTCTAAGGGAAGAGAAGGAGAAGGCAGACAAGTTACTGCTACTTTTGGAAATTGAAAAGGAGAGAACACAGGCTGATGAACTGGCTTCTATTAGATCCATCTTTGATGAAAAATGCATCGAGTTCCAGAAGCTTCTAATGGATGAAAGGCAGAAGGTAGAGGATTCAATGCGACTATTGAAAATTCAAAAGGTGATAACGCAATCACACGCTAATCAACTGGCTTTCAGATCTGCCACAGAGGAATTTTTGAGGAAAGAAAACCAGGCTTCTAAAAAACTTTTGGAAGTTTTAAAGGCAGAATTGAAGGATGTGAAGAACAGAGAACTGTGTACTTGTGGTCAAGTCTCAGGAGCAACAATCAAACGCAAAAGAGAGAAGAACGAGAAAGAGGAGGAAGCTAACAATGAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

478

Amino Acids

55.16

Weight (kDa)

5.2

Isoelectric Point (pI)

50.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 4 - 144 2e-12 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 90
AccII CGCG 3 cut(s) 355, 371, 952
AciI CCGC 4 cut(s) 31, 71, 90, 355
AclWI GGATC 2 cut(s) 564, 1110
AcsI RAATTY 3 cut(s) 515, 1203, 1265
AcuI CTGAAG 2 cut(s) 429, 504
AdeI CACNNNGTG 1 cut(s) 1011
AfaI GTAC 6 cut(s) 14, 260, 461, 578, 841, 1354
AfiI CCNNNNNNNGG 2 cut(s) 59, 250
AflII CTTAAG 1 cut(s) 655
AflIII ACRYGT 2 cut(s) 255, 369
AgeI ACCGGT 1 cut(s) 505
AjiI CACGTC 1 cut(s) 360
AjnI CCWGG 3 cut(s) 168, 957, 1284
AluBI AGCT 8 cut(s) 166, 718, 757, 797, 892, 1021, 1153, 1421
AluI AGCT 8 cut(s) 166, 718, 757, 797, 892, 1021, 1153, 1421
Alw21I GWGCWC 3 cut(s) 28, 344, 1007
Alw26I GTCTC 2 cut(s) 544, 1372
Alw44I GTGCAC 1 cut(s) 340
AlwI GGATC 2 cut(s) 564, 1110
AlwNI CAGNNNCTG 2 cut(s) 10, 118
AoxI GGCC 2 cut(s) 171, 332
ApaLI GTGCAC 1 cut(s) 340
ApeKI GCWGC 2 cut(s) 130, 413
ApoI RAATTY 3 cut(s) 515, 1203, 1265
AsiGI ACCGGT 1 cut(s) 505
AspLEI GCGC 3 cut(s) 108, 117, 607
AspS9I GGNCC 3 cut(s) 172, 332, 855
AsuHPI GGTGA 2 cut(s) 495, 1225
AvaII GGWCC 1 cut(s) 855
BaeGI GKGCMC 2 cut(s) 344, 365
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 3 cut(s) 28, 344, 1007
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 142, 425
BccI CCATC 5 cut(s) 254, 718, 733, 892, 1127
BceAI ACGGC 1 cut(s) 44
BciT130I CCWGG 3 cut(s) 170, 959, 1286
BcoDI GTCTC 2 cut(s) 544, 1372
BfaI CTAG 2 cut(s) 210, 221
BfoI RGCGCY 1 cut(s) 118
BfrI CTTAAG 1 cut(s) 655
BglII AGATCT 2 cut(s) 983, 1250
BisI GCNGC 3 cut(s) 91, 131, 414
BlsI GCNGC 3 cut(s) 92, 132, 415
Bme1390I CCNGG 3 cut(s) 170, 959, 1286
Bme18I GGWCC 1 cut(s) 855
BmgBI CACGTC 1 cut(s) 360
BmgT120I GGNCC 3 cut(s) 172, 332, 855
BmiI GGNNCC 2 cut(s) 333, 785
BmrFI CCNGG 3 cut(s) 170, 959, 1286
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 7 cut(s) 53, 102, 293, 309, 382, 944, 1146
BmuI ACTGGG 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 166, 344
Bsa29I ATCGAT 1 cut(s) 648
BsaWI WCCGGW 1 cut(s) 505
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 250
Bse118I RCCGGY 1 cut(s) 505
Bse1I ACTGG 4 cut(s) 319, 975, 1107, 1245
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 3 cut(s) 170, 959, 1286
BseCI ATCGAT 1 cut(s) 648
BseGI GGATG 8 cut(s) 68, 308, 324, 397, 400, 907, 1168, 1336
BseLI CCNNNNNNNGG 2 cut(s) 59, 250
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 3 cut(s) 77, 125, 1383
BseNI ACTGG 4 cut(s) 319, 975, 1107, 1245
BseRI GAGGAG 4 cut(s) 611, 746, 935, 1427
BseSI GKGCMC 2 cut(s) 344, 365
BseXI GCAGC 2 cut(s) 142, 425
Bsh1236I CGCG 3 cut(s) 355, 371, 952
BshFI GGCC 2 cut(s) 173, 334
BshTI ACCGGT 1 cut(s) 505
BshVI ATCGAT 1 cut(s) 648
BsiHKAI GWGCWC 3 cut(s) 28, 344, 1007
BsiSI CCGG 2 cut(s) 492, 506
BslI CCNNNNNNNGG 2 cut(s) 59, 250
BsmAI GTCTC 2 cut(s) 544, 1372
BsnI GGCC 2 cut(s) 173, 334
Bsp1286I GDGCHC 4 cut(s) 28, 344, 365, 1007
Bsp1407I TGTACA 2 cut(s) 12, 258
Bsp143I GATC 4 cut(s) 556, 983, 1115, 1250
BspACI CCGC 4 cut(s) 31, 71, 90, 355
BspANI GGCC 2 cut(s) 173, 334
BspCNI CTCAG 3 cut(s) 78, 124, 1382
BspDI ATCGAT 1 cut(s) 648
BspFNI CGCG 3 cut(s) 355, 371, 952
BspLI GGNNCC 2 cut(s) 333, 785
BspPI GGATC 2 cut(s) 564, 1110
BspTI CTTAAG 1 cut(s) 655
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 505
BsrGI TGTACA 2 cut(s) 12, 258
BsrI ACTGG 4 cut(s) 319, 975, 1107, 1245
BssAI RCCGGY 1 cut(s) 505
BssMI GATC 4 cut(s) 556, 983, 1115, 1250
Bst2UI CCWGG 3 cut(s) 170, 959, 1286
Bst4CI ACNGT 6 cut(s) 17, 146, 346, 451, 1350, 1434
Bst6I CTCTTC 2 cut(s) 723, 1026
BstAFI CTTAAG 1 cut(s) 655
BstAUI TGTACA 2 cut(s) 12, 258
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 5 cut(s) 86, 111, 980, 1025, 1369
BstF5I GGATG 8 cut(s) 68, 308, 324, 397, 400, 907, 1168, 1336
BstFNI CGCG 3 cut(s) 355, 371, 952
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 3 cut(s) 108, 117, 607
BstKTI GATC 4 cut(s) 559, 986, 1118, 1253
BstMAI GTCTC 2 cut(s) 544, 1372
BstMBI GATC 4 cut(s) 556, 983, 1115, 1250
BstMWI GCNNNNNNNGC 3 cut(s) 112, 114, 437
BstNI CCWGG 3 cut(s) 170, 959, 1286
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 3 cut(s) 168, 957, 1284
BstSLI GKGCMC 2 cut(s) 344, 365
BstUI CGCG 3 cut(s) 355, 371, 952
BstV1I GCAGC 2 cut(s) 142, 425
BstV2I GAAGAC 1 cut(s) 41
BstX2I RGATCY 3 cut(s) 983, 1115, 1250
BstYI RGATCY 3 cut(s) 983, 1115, 1250
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 2 cut(s) 173, 334
BsuTUI ATCGAT 1 cut(s) 648
BtgZI GCGATG 1 cut(s) 967
BtrI CACGTC 1 cut(s) 360
BtsCI GGATG 8 cut(s) 68, 308, 324, 397, 400, 907, 1168, 1336
BtsIMutI CAGTG 2 cut(s) 83, 351
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 2 cut(s) 10, 118
CfoI GCGC 3 cut(s) 108, 117, 607
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 3 cut(s) 172, 332, 855
ClaI ATCGAT 1 cut(s) 648
CseI GACGC 4 cut(s) 112, 360, 377, 958
Csp6I GTAC 6 cut(s) 13, 259, 460, 577, 840, 1353
CspAI ACCGGT 1 cut(s) 505
CviAII CATG 4 cut(s) 37, 250, 256, 296
CviQI GTAC 6 cut(s) 13, 259, 460, 577, 840, 1353
DdeI CTNAG 5 cut(s) 86, 111, 980, 1025, 1369
DpnI GATC 4 cut(s) 558, 985, 1117, 1252
DpnII GATC 4 cut(s) 556, 983, 1115, 1250
DraI TTTAAA 1 cut(s) 1314
DraIII CACNNNGTG 1 cut(s) 1011
Eam1104I CTCTTC 2 cut(s) 723, 1026
EarI CTCTTC 2 cut(s) 723, 1026
Eco47I GGWCC 1 cut(s) 855
Eco57I CTGAAG 2 cut(s) 429, 504
EcoO109I RGGNCCY 3 cut(s) 172, 332, 855
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 3 cut(s) 168, 957, 1284
EcoT22I ATGCAT 2 cut(s) 878, 1139
FaeI CATG 4 cut(s) 40, 253, 259, 299
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FatI CATG 4 cut(s) 36, 249, 255, 295
Fnu4HI GCNGC 3 cut(s) 91, 131, 414
FokI GGATG 8 cut(s) 75, 315, 331, 387, 404, 914, 1175, 1343
Fsp4HI GCNGC 3 cut(s) 91, 131, 414
FspBI CTAG 2 cut(s) 210, 221
GlaI GCGC 3 cut(s) 107, 116, 606
GluI GCNGC 3 cut(s) 91, 131, 414
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 2 cut(s) 173, 334
HapII CCGG 2 cut(s) 492, 506
HgaI GACGC 4 cut(s) 112, 360, 377, 958
HhaI GCGC 3 cut(s) 108, 117, 607
Hin1II CATG 4 cut(s) 40, 253, 259, 299
Hin6I GCGC 3 cut(s) 106, 115, 605
HinP1I GCGC 3 cut(s) 106, 115, 605
HindIII AAGCTT 4 cut(s) 716, 795, 890, 1151
HinfI GANTC 4 cut(s) 671, 881, 923, 1184
HpaII CCGG 2 cut(s) 492, 506
HphI GGTGA 2 cut(s) 495, 1225
Hpy166II GTNNAC 3 cut(s) 342, 532, 1353
Hpy188I TCNGA 9 cut(s) 62, 150, 484, 514, 544, 723, 852, 897, 1250
Hpy188III TCNNGA 7 cut(s) 554, 560, 679, 688, 764, 1147, 1371
Hpy8I GTNNAC 3 cut(s) 342, 532, 1353
Hpy99I CGWCG 2 cut(s) 371, 376
HpyAV CCTTC 8 cut(s) 77, 185, 293, 907, 1030, 1036, 1168, 1321
HpyCH4III ACNGT 6 cut(s) 17, 146, 346, 451, 1350, 1434
HpyCH4IV ACGT 2 cut(s) 359, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 114, 437
HpyF3I CTNAG 5 cut(s) 86, 111, 980, 1025, 1369
HpySE526I ACGT 2 cut(s) 359, 500
Hsp92II CATG 4 cut(s) 40, 253, 259, 299
HspAI GCGC 3 cut(s) 106, 115, 605
Kzo9I GATC 4 cut(s) 556, 983, 1115, 1250
LmnI GCTCC 5 cut(s) 23, 171, 733, 1018, 1373
Lsp1109I GCAGC 2 cut(s) 142, 425
LweI GCATC 7 cut(s) 53, 102, 293, 309, 382, 944, 1146
MaeI CTAG 2 cut(s) 210, 221
MaeII ACGT 2 cut(s) 359, 500
MaeIII GTNAC 3 cut(s) 124, 501, 1053
MalI GATC 4 cut(s) 558, 985, 1117, 1252
MbiI CCGCTC 1 cut(s) 90
MboI GATC 4 cut(s) 556, 983, 1115, 1250
MboII GAAGA 8 cut(s) 41, 575, 612, 740, 758, 1043, 1348, 1411
MflI RGATCY 3 cut(s) 983, 1115, 1250
MhlI GDGCHC 4 cut(s) 28, 344, 365, 1007
MluI ACGCGT 1 cut(s) 369
MlyI GAGTC 2 cut(s) 890, 932
Mph1103I ATGCAT 2 cut(s) 878, 1139
MseI TTAA 4 cut(s) 656, 888, 992, 1313
MslI CAYNNNNRTG 3 cut(s) 254, 294, 524
MspCI CTTAAG 1 cut(s) 655
MspI CCGG 2 cut(s) 492, 506
MspR9I CCNGG 3 cut(s) 170, 959, 1286
MvaI CCWGG 3 cut(s) 170, 959, 1286
MvnI CGCG 3 cut(s) 355, 371, 952
MwoI GCNNNNNNNGC 3 cut(s) 112, 114, 437
NdeII GATC 4 cut(s) 556, 983, 1115, 1250
NlaIII CATG 4 cut(s) 40, 253, 259, 299
NlaIV GGNNCC 2 cut(s) 333, 785
NmuCI GTSAC 2 cut(s) 124, 501
NsiI ATGCAT 2 cut(s) 878, 1139
NspI RCATGY 1 cut(s) 259
PciI ACATGT 1 cut(s) 255
PfeI GAWTC 2 cut(s) 671, 1184
PflFI GACNNNGTC 1 cut(s) 536
PinAI ACCGGT 1 cut(s) 505
PkrI GCNGC 3 cut(s) 92, 132, 415
PleI GAGTC 2 cut(s) 889, 931
PpsI GAGTC 2 cut(s) 889, 931
PpuMI RGGWCCY 1 cut(s) 855
PscI ACATGT 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 855
Psp6I CCWGG 3 cut(s) 168, 957, 1284
PspGI CCWGG 3 cut(s) 168, 957, 1284
PspN4I GGNNCC 2 cut(s) 333, 785
PspPI GGNCC 3 cut(s) 172, 332, 855
PspPPI RGGWCCY 1 cut(s) 855
PstNI CAGNNNCTG 2 cut(s) 10, 118
PsuI RGATCY 3 cut(s) 983, 1115, 1250
PsyI GACNNNGTC 1 cut(s) 536
RsaI GTAC 6 cut(s) 14, 260, 461, 578, 841, 1354
RsaNI GTAC 6 cut(s) 13, 259, 460, 577, 840, 1353
RseI CAYNNNNRTG 3 cut(s) 254, 294, 524
SaqAI TTAA 4 cut(s) 656, 888, 992, 1313
SatI GCNGC 3 cut(s) 91, 131, 414
Sau3AI GATC 4 cut(s) 556, 983, 1115, 1250
Sau96I GGNCC 3 cut(s) 172, 332, 855
SchI GAGTC 2 cut(s) 890, 932
ScrFI CCNGG 3 cut(s) 170, 959, 1286
SduI GDGCHC 4 cut(s) 28, 344, 365, 1007
SfaNI GCATC 7 cut(s) 53, 102, 293, 309, 382, 944, 1146
SgrAI CRCCGGYG 1 cut(s) 505
SinI GGWCC 1 cut(s) 855
SmiMI CAYNNNNRTG 3 cut(s) 254, 294, 524
SmlI CTYRAG 3 cut(s) 181, 323, 655
SmoI CTYRAG 3 cut(s) 181, 323, 655
SsiI CCGC 4 cut(s) 31, 71, 90, 355
SspMI CTAG 2 cut(s) 210, 221
StyD4I CCNGG 3 cut(s) 168, 957, 1284
TaaI ACNGT 6 cut(s) 17, 146, 346, 451, 1350, 1434
TaiI ACGT 2 cut(s) 362, 503
TaqI TCGA 3 cut(s) 374, 648, 1140
TaqII GACCGA 1 cut(s) 468
TatI WGTACW 4 cut(s) 12, 258, 576, 1352
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 2 cut(s) 671, 1184
Tru1I TTAA 4 cut(s) 656, 888, 992, 1313
Tru9I TTAA 4 cut(s) 656, 888, 992, 1313
TscAI CASTG 2 cut(s) 90, 351
TseFI GTSAC 2 cut(s) 124, 501
TseI GCWGC 2 cut(s) 130, 413
Tsp45I GTSAC 2 cut(s) 124, 501
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 2 cut(s) 90, 351
Tth111I GACNNNGTC 1 cut(s) 536
Vha464I CTTAAG 1 cut(s) 655
VneI GTGCAC 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 855
XapI RAATTY 3 cut(s) 515, 1203, 1265
XceI RCATGY 1 cut(s) 259
XspI CTAG 2 cut(s) 210, 221
Zsp2I ATGCAT 2 cut(s) 878, 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.