AT2G30220

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001786
Physical Location & Seq
Reverse (-)
12891233 .. 12892666
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30220.1

Sequence Viewer

Length: 1077 bp
ATGTATATATCTAAAACCATAGTGTTTGGTCTCTTTGTTGCAACACTCCTCGTGTCTTGCAATGCGGATGCAAACACCACGCAGCCGCTTTTCCCGGCGATTCTAATATTCGGCGACTCAACGGCAGACACAGGCAATAACAACTACTACTCACAAGCCGTCTTCAAGGCTAATCACCTCCCTTATGGCGTTGATCTTCCCGGCCACGAAGCTAACGGAAGATTCTCAAACGGAAAGCTAATCTCTGACGTAATCTCCACAAAACTCAACATCAAAGAGTTTGTTCCTCCGTTTCTACAACCGAACATCTCCGACCAAGACATTGTCACTGGAGTCTGTTTTGCATCCGCGGGTGCTGGCTACGATGACGAGACCTCACTATCTAGCAAAGCTATTCCGGTCTCACAACAGCCAAGCATGTTCAAGAATTATATTGCTCGTCTCAAAGGTATCGTAGGAGACAAGAAAGCGATGGAGATTATTAACAACGCTTTAGTGGTCATAAGCGCAGGGCCTAATGATTTCATCTTGAACTTTTACGATATCCCCATAAGGCGTCTCGAGTATCCTACTATCTATGGCTACCAAGATTTTGTTCTCAAGAGGCTTGACGGTTTTGTCCGGGAGCTTTATAGTTTAGGTTGCCGGAATATTCTAGTCGGAGGGTTGCCGCCAATGGGATGTTTACCGATCCAATTGACCGCGAAATTGAGAACCATTTTGGGAATTTGTGTGGAACAAGAAAACAAAGACTCCATTTTGTACAATCAGAAGCTTGTAAAGAAATTGCCTGAGATCCAAGCGTCTCTACCCGGAAGCAAGTTCCTTTACGCTAACGTCTACGACCCTGTGATGGACATGATCCGAAACCCTAGCAAATACGGATTCAAGGAGACGAAGAAAGGATGTTGCGGAACAGGATACTTGGAAACAAGCTTCTTGTGCACTTCCTTGTCAAAGACTTGTCCGAATCATTCGGATCACTTGTTTTGGGACTCCATTCATCCCTCCGAGGCAGCTTACAAATACCTAGGGAACTTCATAGATGCTCAGATTCAAGAGTGGCTTAAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

358

Amino Acids

39.67

Weight (kDa)

6.93

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 346 8.3e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
AccI GTMKAC 1 cut(s) 840
AccII CGCG 2 cut(s) 350, 704
AciI CCGC 7 cut(s) 65, 86, 348, 350, 671, 702, 912
AclWI GGATC 4 cut(s) 685, 790, 856, 987
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 1 cut(s) 726
AcyI GRCGYC 1 cut(s) 556
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 2 cut(s) 677, 853
AflII CTTAAG 1 cut(s) 1067
AgsI TTSAA 5 cut(s) 166, 424, 532, 889, 1058
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AluBI AGCT 7 cut(s) 212, 238, 392, 628, 775, 936, 1019
AluI AGCT 7 cut(s) 212, 238, 392, 628, 775, 936, 1019
Alw21I GWGCWC 1 cut(s) 947
Alw26I GTCTC 8 cut(s) 35, 365, 406, 446, 453, 563, 810, 887
Alw44I GTGCAC 1 cut(s) 943
AlwI GGATC 4 cut(s) 685, 790, 856, 987
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 2 cut(s) 202, 512
ApaLI GTGCAC 1 cut(s) 943
ApeKI GCWGC 2 cut(s) 82, 1016
ApoI RAATTY 1 cut(s) 726
AspA2I CCTAGG 1 cut(s) 1030
AspLEI GCGC 1 cut(s) 509
AspS9I GGNCC 1 cut(s) 512
AsuC2I CCSGG 4 cut(s) 95, 201, 623, 813
AsuHPI GGTGA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 560
AvrII CCTAGG 1 cut(s) 1030
BaeGI GKGCMC 1 cut(s) 947
BauI CACGAG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 947
BbvI GCAGC 2 cut(s) 94, 1028
BccI CCATC 2 cut(s) 466, 847
BceAI ACGGC 2 cut(s) 138, 143
BciVI GTATCC 2 cut(s) 576, 914
BcnI CCSGG 4 cut(s) 95, 201, 623, 813
BcoDI GTCTC 8 cut(s) 35, 365, 406, 446, 453, 563, 810, 887
BfaI CTAG 4 cut(s) 384, 656, 873, 1031
BfrI CTTAAG 1 cut(s) 1067
BfuI GTATCC 2 cut(s) 576, 914
BisI GCNGC 4 cut(s) 83, 86, 671, 1017
BlnI CCTAGG 1 cut(s) 1030
BlsI GCNGC 4 cut(s) 84, 87, 672, 1018
Bme1390I CCNGG 4 cut(s) 95, 201, 623, 813
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 512
BmrFI CCNGG 4 cut(s) 95, 201, 623, 813
BmsI GCATC 3 cut(s) 58, 353, 1036
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 4 cut(s) 95, 201, 623, 813
BsaHI GRCGYC 1 cut(s) 556
BsaI GGTCTC 3 cut(s) 35, 365, 406
BsaJI CCNNGG 3 cut(s) 348, 1011, 1030
BsaWI WCCGGW 1 cut(s) 397
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 737, 767
Bsc4I CCNNNNNNNGG 2 cut(s) 677, 853
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 67
BseDI CCNNGG 3 cut(s) 348, 1011, 1030
BseGI GGATG 5 cut(s) 73, 344, 686, 911, 1003
BseLI CCNNNNNNNGG 2 cut(s) 677, 853
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 2 cut(s) 783, 1064
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 2 cut(s) 94, 1028
Bsh1236I CGCG 2 cut(s) 350, 704
BshFI GGCC 2 cut(s) 204, 514
BsiHKAI GWGCWC 1 cut(s) 947
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 6 cut(s) 95, 201, 398, 622, 646, 813
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 2 cut(s) 677, 853
BsmAI GTCTC 8 cut(s) 35, 365, 406, 446, 453, 563, 810, 887
BsmBI CGTCTC 4 cut(s) 446, 563, 810, 887
BsmFI GGGAC 1 cut(s) 1007
BsnI GGCC 2 cut(s) 204, 514
Bso31I GGTCTC 3 cut(s) 35, 365, 406
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 947
Bsp1407I TGTACA 1 cut(s) 762
Bsp143I GATC 5 cut(s) 193, 690, 795, 861, 979
BspACI CCGC 7 cut(s) 65, 86, 348, 350, 671, 702, 912
BspANI GGCC 2 cut(s) 204, 514
BspCNI CTCAG 2 cut(s) 784, 1063
BspFNI CGCG 2 cut(s) 350, 704
BspPI GGATC 4 cut(s) 685, 790, 856, 987
BspTI CTTAAG 1 cut(s) 1067
BspTNI GGTCTC 3 cut(s) 35, 365, 406
BsrDI GCAATG 1 cut(s) 67
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 3 cut(s) 348, 1011, 1030
BssMI GATC 5 cut(s) 193, 690, 795, 861, 979
BssNI GRCGYC 1 cut(s) 556
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 1030
Bst2BI CACGAG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 614
BstACI GRCGYC 1 cut(s) 556
BstAFI CTTAAG 1 cut(s) 1067
BstAUI TGTACA 1 cut(s) 762
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 2 cut(s) 792, 1050
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 5 cut(s) 73, 344, 686, 911, 1003
BstFNI CGCG 2 cut(s) 350, 704
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 5 cut(s) 196, 693, 798, 864, 982
BstMAI GTCTC 8 cut(s) 35, 365, 406, 446, 453, 563, 810, 887
BstMBI GATC 5 cut(s) 193, 690, 795, 861, 979
BstMWI GCNNNNNNNGC 1 cut(s) 942
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 4 cut(s) 93, 199, 621, 811
BstSLI GKGCMC 1 cut(s) 947
BstUI CGCG 2 cut(s) 350, 704
BstV1I GCAGC 2 cut(s) 94, 1028
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 795
BstYI RGATCY 1 cut(s) 795
BsuI GTATCC 2 cut(s) 576, 914
BsuRI GGCC 2 cut(s) 204, 514
BtgI CCRYGG 1 cut(s) 348
BtgZI GCGATG 1 cut(s) 485
BtsCI GGATG 5 cut(s) 73, 344, 686, 911, 1003
BtsIMutI CAGTG 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 358
CfoI GCGC 1 cut(s) 509
Cfr13I GGNCC 1 cut(s) 512
Cfr42I CCGCGG 1 cut(s) 351
CseI GACGC 2 cut(s) 545, 792
Csp6I GTAC 1 cut(s) 763
CviAII CATG 2 cut(s) 418, 859
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 2 cut(s) 792, 1050
DpnI GATC 5 cut(s) 195, 692, 797, 863, 981
DpnII GATC 5 cut(s) 193, 690, 795, 861, 979
DrdI GACNNNNNNGTC 1 cut(s) 617
DseDI GACNNNNNNGTC 1 cut(s) 617
EaeI YGGCCR 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 1030
Eco31I GGTCTC 3 cut(s) 35, 365, 406
Eco32I GATATC 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 512
EcoRV GATATC 1 cut(s) 544
EcoT14I CCWWGG 1 cut(s) 1030
ErhI CCWWGG 1 cut(s) 1030
Esp3I CGTCTC 4 cut(s) 446, 563, 810, 887
FaeI CATG 2 cut(s) 421, 862
FalI AAGNNNNNCTT 1 cut(s) 1050
FaqI GGGAC 1 cut(s) 1007
FatI CATG 2 cut(s) 417, 858
FauI CCCGC 1 cut(s) 343
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 4 cut(s) 83, 86, 671, 1017
FokI GGATG 5 cut(s) 80, 331, 693, 918, 990
Fsp4HI GCNGC 4 cut(s) 83, 86, 671, 1017
FspBI CTAG 4 cut(s) 384, 656, 873, 1031
GlaI GCGC 1 cut(s) 508
GluI GCNGC 4 cut(s) 83, 86, 671, 1017
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 2 cut(s) 204, 514
HapII CCGG 6 cut(s) 95, 201, 398, 622, 646, 813
HgaI GACGC 2 cut(s) 545, 792
HhaI GCGC 1 cut(s) 509
Hin1I GRCGYC 1 cut(s) 556
Hin1II CATG 2 cut(s) 421, 862
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HindIII AAGCTT 2 cut(s) 773, 934
HinfI GANTC 9 cut(s) 100, 116, 222, 333, 752, 885, 970, 995, 1054
HpaII CCGG 6 cut(s) 95, 201, 398, 622, 646, 813
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 3 cut(s) 686, 841, 945
Hpy188I TCNGA 9 cut(s) 247, 313, 662, 771, 866, 969, 979, 1012, 1053
Hpy188III TCNNGA 5 cut(s) 424, 529, 560, 601, 1058
Hpy8I GTNNAC 3 cut(s) 686, 841, 945
HpyCH4III ACNGT 1 cut(s) 614
HpyCH4IV ACGT 2 cut(s) 249, 837
HpyCH4V TGCA 5 cut(s) 41, 60, 71, 344, 945
HpyF10VI GCNNNNNNNGC 1 cut(s) 942
HpyF3I CTNAG 2 cut(s) 792, 1050
HpySE526I ACGT 2 cut(s) 249, 837
Hsp92I GRCGYC 1 cut(s) 556
Hsp92II CATG 2 cut(s) 421, 862
HspAI GCGC 1 cut(s) 507
KspI CCGCGG 1 cut(s) 351
Kzo9I GATC 5 cut(s) 193, 690, 795, 861, 979
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 2 cut(s) 94, 1028
LweI GCATC 3 cut(s) 58, 353, 1036
MaeI CTAG 4 cut(s) 384, 656, 873, 1031
MaeII ACGT 2 cut(s) 249, 837
MaeIII GTNAC 1 cut(s) 325
MalI GATC 5 cut(s) 195, 692, 797, 863, 981
MboI GATC 5 cut(s) 193, 690, 795, 861, 979
MboII GAAGA 4 cut(s) 154, 188, 231, 910
MfeI CAATTG 1 cut(s) 695
MflI RGATCY 1 cut(s) 795
MhlI GDGCHC 1 cut(s) 947
MluCI AATT 5 cut(s) 427, 695, 707, 726, 785
MlyI GAGTC 4 cut(s) 110, 342, 746, 989
MmeI TCCRAC 2 cut(s) 336, 640
MnlI CCTC 8 cut(s) 59, 188, 297, 385, 597, 656, 1006, 1018
MseI TTAA 3 cut(s) 483, 1068, 1075
MspA1I CMGCKG 1 cut(s) 350
MspCI CTTAAG 1 cut(s) 1067
MspI CCGG 6 cut(s) 95, 201, 398, 622, 646, 813
MspR9I CCNGG 4 cut(s) 95, 201, 623, 813
MunI CAATTG 1 cut(s) 695
MvnI CGCG 2 cut(s) 350, 704
MwoI GCNNNNNNNGC 1 cut(s) 942
NciI CCSGG 4 cut(s) 95, 201, 623, 813
NdeII GATC 5 cut(s) 193, 690, 795, 861, 979
NlaIII CATG 2 cut(s) 421, 862
NmuCI GTSAC 1 cut(s) 325
NspI RCATGY 1 cut(s) 421
PaeR7I CTCGAG 1 cut(s) 560
PfeI GAWTC 5 cut(s) 100, 222, 885, 970, 1054
PflFI GACNNNGTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 621
PkrI GCNGC 4 cut(s) 84, 87, 672, 1018
PleI GAGTC 4 cut(s) 110, 341, 746, 989
PpsI GAGTC 4 cut(s) 110, 341, 746, 989
PspPI GGNCC 1 cut(s) 512
PsuI RGATCY 1 cut(s) 795
PsyI GACNNNGTC 1 cut(s) 323
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
SacII CCGCGG 1 cut(s) 351
SaqAI TTAA 3 cut(s) 483, 1068, 1075
SatI GCNGC 4 cut(s) 83, 86, 671, 1017
Sau3AI GATC 5 cut(s) 193, 690, 795, 861, 979
Sau96I GGNCC 1 cut(s) 512
SchI GAGTC 4 cut(s) 110, 342, 746, 989
ScrFI CCNGG 4 cut(s) 95, 201, 623, 813
SduI GDGCHC 1 cut(s) 947
SfaNI GCATC 3 cut(s) 58, 353, 1036
Sfr274I CTCGAG 1 cut(s) 560
Sfr303I CCGCGG 1 cut(s) 351
SgrBI CCGCGG 1 cut(s) 351
SlaI CTCGAG 1 cut(s) 560
SmlI CTYRAG 3 cut(s) 560, 599, 1067
SmoI CTYRAG 3 cut(s) 560, 599, 1067
Sse9I AATT 5 cut(s) 427, 695, 707, 726, 785
SsiI CCGC 7 cut(s) 65, 86, 348, 350, 671, 702, 912
SspI AATATT 2 cut(s) 108, 652
SspMI CTAG 4 cut(s) 384, 656, 873, 1031
StyD4I CCNGG 4 cut(s) 93, 199, 621, 811
StyI CCWWGG 1 cut(s) 1030
TaaI ACNGT 1 cut(s) 614
TaiI ACGT 2 cut(s) 252, 840
TaqI TCGA 1 cut(s) 561
TasI AATT 5 cut(s) 427, 695, 707, 726, 785
TatI WGTACW 1 cut(s) 762
TauI GCSGC 2 cut(s) 88, 673
TfiI GAWTC 5 cut(s) 100, 222, 885, 970, 1054
Tru1I TTAA 3 cut(s) 483, 1068, 1075
Tru9I TTAA 3 cut(s) 483, 1068, 1075
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 2 cut(s) 82, 1016
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 3 cut(s) 514, 992, 1030
TspGWI ACGGA 4 cut(s) 231, 246, 279, 897
TspRI CASTG 1 cut(s) 334
Tth111I GACNNNGTC 1 cut(s) 323
Vha464I CTTAAG 1 cut(s) 1067
VneI GTGCAC 1 cut(s) 943
XapI RAATTY 1 cut(s) 726
XceI RCATGY 1 cut(s) 421
XhoI CTCGAG 1 cut(s) 560
XmaJI CCTAGG 1 cut(s) 1030
XmiI GTMKAC 1 cut(s) 840
XspI CTAG 4 cut(s) 384, 656, 873, 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.