AT2G31540

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001786
Physical Location & Seq
Reverse (-)
13430469 .. 13432065
1597 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31540.1

Sequence Viewer

Length: 1083 bp
ATGTCGACTTCTAAAGCCATAACCTTAACTCTCTTCATTGCAACAACATTACTAGCGCCCTGCAACGCAGCCGCAAACGCAACAACGAAGCCGCTCTTCCCAGCGATTCTAATCTTCGGTGATTCAACCGTCGATACAGGAAACAACAACTACCCTTTACCAACAATCTTCAGAGCTGAACACTTTCCTTACGGCATGGATCTTCCAGACGGAAAAGCTAACGGAAGATTCTCAAACGGAAAACTTATCTCCGACATAATCGCAACCAAACTCAACATCAAAGAGTTTATTCCTCCTTTCTTACAACCAAATCTCTCCGACCAAGACATTCTAACCGGAGTCTGTTTCGCATCAGCTGGTGCCGGTTACGACGACCTGACCAGTCTCTCGACACAAGCCATTCGTGTCTCGGAACAACCAAACATGTTCAAGAGTTACATTGCTCGTCTCAAAGGTATCGTGGGAGACAAGAAAGCGATGGAGATCATAAACAATGCCTTCGTGGTTGTGAGTGCAGGGCCTAACGATTTCATTTTGAACTATTACGAAATTCCCTCAAGGCGTCTCGAGTATCCTTTCATTTCTGGTTACCAAGACTTTATTCTTAAGAGGCTTGAAAATTTCGTGCGGGAGCTTTACAGTTTAGGTGTCCGGAATGTTTTGGTTGGAGGTTTACCGCCAATGGGGTGTTTACCGATCCATATGACTGCTAAATTCCGCAACATTTTTAGATTCTGCTTGGAACACCATAACAAAGACTCTGTTTTATACAATGAGAAACTTCAGAATCTCTTGCCCCAAATCGAAGCATCTCTTCCAGGAAGCAAATTCCTTTACGCCGATGTCTATAATCCTATGATGGAGATGATCCAAAACCCTAGCAAATACGGGTTCAAAGAGACGAAGAGAGGATGTTGTGGAACAGGGTTCCTGGAGACGAGCTTCATGTGTAATGTTTTTTCTCCTGTGTGTCAAAATCGGTCGGAGTTTTTGTTCTTTGACTCGATTCATCCATCGGAAGCTACCTATAATGTCATAGGCAATCTTCTGGATCCTCTAATTCGTGGAAAGTTCCAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

360

Amino Acids

40.12

Weight (kDa)

7.54

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 349 3.2e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 94
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 651
AciI CCGC 5 cut(s) 72, 92, 628, 677, 718
AclWI GGATC 5 cut(s) 207, 691, 862, 1046, 1059
AcsI RAATTY 4 cut(s) 549, 619, 713, 827
AcuI CTGAAG 2 cut(s) 154, 767
AcyI GRCGYC 1 cut(s) 562
AfiI CCNNNNNNNGG 1 cut(s) 683
AflII CTTAAG 1 cut(s) 605
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 5 cut(s) 126, 430, 538, 617, 895
AjnI CCWGG 3 cut(s) 817, 930, 1074
AjuI GAANNNNNNNTTGG 2 cut(s) 585, 617
AloI GAACNNNNNNTCC 2 cut(s) 977, 1009
AluBI AGCT 6 cut(s) 176, 218, 356, 634, 942, 1022
AluI AGCT 6 cut(s) 176, 218, 356, 634, 942, 1022
Alw26I GTCTC 7 cut(s) 389, 412, 452, 459, 569, 893, 929
AlwI GGATC 5 cut(s) 207, 691, 862, 1046, 1059
Ama87I CYCGRG 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 651
AoxI GGCC 1 cut(s) 518
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 4 cut(s) 549, 619, 713, 827
Asp700I GAANNNNTTC 1 cut(s) 183
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 518
AsuHPI GGTGA 1 cut(s) 131
AvaI CYCGRG 1 cut(s) 566
BamHI GGATCC 1 cut(s) 1051
BanI GGYRCC 1 cut(s) 359
BbvI GCAGC 1 cut(s) 80
BccI CCATC 3 cut(s) 472, 853, 1021
BceAI ACGGC 1 cut(s) 208
BciT130I CCWGG 3 cut(s) 819, 932, 1076
BciVI GTATCC 1 cut(s) 582
BcoDI GTCTC 7 cut(s) 389, 412, 452, 459, 569, 893, 929
BfaI CTAG 2 cut(s) 53, 879
BfoI RGCGCY 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 605
BfuI GTATCC 1 cut(s) 582
BisI GCNGC 3 cut(s) 69, 72, 92
BlsI GCNGC 3 cut(s) 70, 73, 93
Bme1390I CCNGG 3 cut(s) 819, 932, 1076
BmeT110I CYCGRG 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 518
BmiI GGNNCC 3 cut(s) 361, 929, 1053
BmrFI CCNGG 3 cut(s) 819, 932, 1076
BmsI GCATC 2 cut(s) 359, 818
BpmI CTGGAG 1 cut(s) 953
BpuEI CTTGAG 1 cut(s) 541
BsaBI GATNNNNATC 2 cut(s) 110, 482
BsaHI GRCGYC 1 cut(s) 562
BsaWI WCCGGW 2 cut(s) 335, 651
BsaXI ACNNNNNCTCC 2 cut(s) 977, 1007
Bsc4I CCNNNNNNNGG 1 cut(s) 683
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 381
Bse3DI GCAATG 2 cut(s) 36, 438
Bse8I GATNNNNATC 2 cut(s) 110, 482
BseAI TCCGGA 1 cut(s) 651
BseBI CCWGG 3 cut(s) 819, 932, 1076
BseGI GGATG 2 cut(s) 917, 1009
BseJI GATNNNNATC 2 cut(s) 110, 482
BseLI CCNNNNNNNGG 1 cut(s) 683
BseMI GCAATG 2 cut(s) 36, 438
BseNI ACTGG 1 cut(s) 381
BseXI GCAGC 1 cut(s) 80
BseYI CCCAGC 1 cut(s) 100
BsgI GTGCAG 1 cut(s) 534
Bsh1285I CGRYCG 1 cut(s) 983
BshFI GGCC 1 cut(s) 520
BshNI GGYRCC 1 cut(s) 359
BsiEI CGRYCG 1 cut(s) 983
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 3 cut(s) 336, 363, 652
BslI CCNNNNNNNGG 1 cut(s) 683
BsmAI GTCTC 7 cut(s) 389, 412, 452, 459, 569, 893, 929
BsmBI CGTCTC 4 cut(s) 452, 569, 893, 929
BsnI GGCC 1 cut(s) 520
BsoBI CYCGRG 1 cut(s) 566
Bsp13I TCCGGA 1 cut(s) 651
Bsp143I GATC 5 cut(s) 199, 483, 696, 867, 1051
BspACI CCGC 5 cut(s) 72, 92, 628, 677, 718
BspANI GGCC 1 cut(s) 520
BspEI TCCGGA 1 cut(s) 651
BspLI GGNNCC 3 cut(s) 361, 929, 1053
BspPI GGATC 5 cut(s) 207, 691, 862, 1046, 1059
BspQI GCTCTTC 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 359
BspTI CTTAAG 1 cut(s) 605
BsrBI CCGCTC 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 36, 438
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 1 cut(s) 381
BssAI RCCGGY 1 cut(s) 362
BssMI GATC 5 cut(s) 199, 483, 696, 867, 1051
BssNI GRCGYC 1 cut(s) 562
Bst2UI CCWGG 3 cut(s) 819, 932, 1076
Bst4CI ACNGT 2 cut(s) 130, 641
Bst6I CTCTTC 4 cut(s) 38, 101, 819, 899
BstACI GRCGYC 1 cut(s) 562
BstAFI CTTAAG 1 cut(s) 605
BstEII GGTNACC 1 cut(s) 587
BstF5I GGATG 2 cut(s) 917, 1009
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 5 cut(s) 202, 486, 699, 870, 1054
BstMAI GTCTC 7 cut(s) 389, 412, 452, 459, 569, 893, 929
BstMBI GATC 5 cut(s) 199, 483, 696, 867, 1051
BstMCI CGRYCG 1 cut(s) 983
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 3 cut(s) 819, 932, 1076
BstNSI RCATGY 1 cut(s) 427
BstPI GGTNACC 1 cut(s) 587
BstSCI CCNGG 3 cut(s) 817, 930, 1074
BstV1I GCAGC 1 cut(s) 80
BstX2I RGATCY 2 cut(s) 199, 1051
BstYI RGATCY 2 cut(s) 199, 1051
BsuI GTATCC 1 cut(s) 582
BsuRI GGCC 1 cut(s) 520
BtgZI GCGATG 1 cut(s) 491
BtsCI GGATG 2 cut(s) 917, 1009
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 518
CseI GACGC 1 cut(s) 551
CviAII CATG 3 cut(s) 196, 424, 946
DpnI GATC 5 cut(s) 201, 485, 698, 869, 1053
DpnII GATC 5 cut(s) 199, 483, 696, 867, 1051
Eam1104I CTCTTC 4 cut(s) 38, 101, 819, 899
EarI CTCTTC 4 cut(s) 38, 101, 819, 899
Eco57I CTGAAG 2 cut(s) 154, 767
Eco88I CYCGRG 1 cut(s) 566
Eco91I GGTNACC 1 cut(s) 587
EcoO109I RGGNCCY 1 cut(s) 518
EcoO65I GGTNACC 1 cut(s) 587
EcoRII CCWGG 3 cut(s) 817, 930, 1074
Esp3I CGTCTC 4 cut(s) 452, 569, 893, 929
FaeI CATG 3 cut(s) 199, 427, 949
FalI AAGNNNNNCTT 4 cut(s) 80, 112, 798, 830
FatI CATG 3 cut(s) 195, 423, 945
FauI CCCGC 1 cut(s) 621
FauNDI CATATG 1 cut(s) 702
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 69, 72, 92
FokI GGATG 2 cut(s) 924, 996
Fsp4HI GCNGC 3 cut(s) 69, 72, 92
FspBI CTAG 2 cut(s) 53, 879
GlaI GCGC 1 cut(s) 57
GluI GCNGC 3 cut(s) 69, 72, 92
GsaI CCCAGC 1 cut(s) 104
GsuI CTGGAG 1 cut(s) 953
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 1 cut(s) 520
HapII CCGG 3 cut(s) 336, 363, 652
HgaI GACGC 1 cut(s) 551
HhaI GCGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 562
Hin1II CATG 3 cut(s) 199, 427, 949
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 9 cut(s) 106, 122, 228, 339, 732, 758, 787, 1001, 1006
HpaII CCGG 3 cut(s) 336, 363, 652
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 6, 674, 692
Hpy188I TCNGA 7 cut(s) 173, 253, 319, 412, 786, 985, 1018
Hpy188III TCNNGA 6 cut(s) 206, 388, 430, 566, 652, 1049
Hpy8I GTNNAC 3 cut(s) 6, 674, 692
Hpy99I CGWCG 2 cut(s) 134, 374
HpyAV CCTTC 1 cut(s) 508
HpyCH4III ACNGT 2 cut(s) 130, 641
HpyCH4V TGCA 3 cut(s) 41, 63, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
Hsp92I GRCGYC 1 cut(s) 562
Hsp92II CATG 3 cut(s) 199, 427, 949
HspAI GCGC 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 651
Kzo9I GATC 5 cut(s) 199, 483, 696, 867, 1051
LguI GCTCTTC 1 cut(s) 101
LmnI GCTCC 1 cut(s) 631
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 2 cut(s) 359, 818
MaeI CTAG 2 cut(s) 53, 879
MaeIII GTNAC 3 cut(s) 365, 434, 587
MalI GATC 5 cut(s) 201, 485, 698, 869, 1053
MbiI CCGCTC 1 cut(s) 94
MboI GATC 5 cut(s) 199, 483, 696, 867, 1051
MboII GAAGA 9 cut(s) 25, 88, 106, 160, 194, 237, 806, 916, 1037
MflI RGATCY 2 cut(s) 199, 1051
MluCI AATT 5 cut(s) 549, 619, 713, 827, 1059
MlyI GAGTC 3 cut(s) 348, 752, 995
MmeI TCCRAC 4 cut(s) 276, 342, 646, 963
MnlI CCTC 6 cut(s) 303, 565, 603, 662, 902, 1065
MroI TCCGGA 1 cut(s) 651
MroXI GAANNNNTTC 1 cut(s) 183
MseI TTAA 3 cut(s) 26, 606, 1081
MspA1I CMGCKG 1 cut(s) 356
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 3 cut(s) 336, 363, 652
MspR9I CCNGG 3 cut(s) 819, 932, 1076
MvaI CCWGG 3 cut(s) 819, 932, 1076
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeI CATATG 1 cut(s) 702
NdeII GATC 5 cut(s) 199, 483, 696, 867, 1051
NlaIII CATG 3 cut(s) 199, 427, 949
NlaIV GGNNCC 3 cut(s) 361, 929, 1053
NspI RCATGY 1 cut(s) 427
PaeR7I CTCGAG 1 cut(s) 566
PciI ACATGT 1 cut(s) 423
PciSI GCTCTTC 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 6 cut(s) 106, 122, 228, 732, 787, 1006
PfoI TCCNGGA 2 cut(s) 817, 930
PkrI GCNGC 3 cut(s) 70, 73, 93
PleI GAGTC 3 cut(s) 347, 752, 995
PpsI GAGTC 3 cut(s) 347, 752, 995
PscI ACATGT 1 cut(s) 423
Psp6I CCWGG 3 cut(s) 817, 930, 1074
PspEI GGTNACC 1 cut(s) 587
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 3 cut(s) 817, 930, 1074
PspN4I GGNNCC 3 cut(s) 361, 929, 1053
PspPI GGNCC 1 cut(s) 518
PsuI RGATCY 2 cut(s) 199, 1051
PvuII CAGCTG 1 cut(s) 356
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 101
SaqAI TTAA 3 cut(s) 26, 606, 1081
SatI GCNGC 3 cut(s) 69, 72, 92
Sau3AI GATC 5 cut(s) 199, 483, 696, 867, 1051
Sau96I GGNCC 1 cut(s) 518
SchI GAGTC 3 cut(s) 348, 752, 995
ScrFI CCNGG 3 cut(s) 819, 932, 1076
SfaNI GCATC 2 cut(s) 359, 818
Sfr274I CTCGAG 1 cut(s) 566
SlaI CTCGAG 1 cut(s) 566
SmlI CTYRAG 3 cut(s) 556, 566, 605
SmoI CTYRAG 3 cut(s) 556, 566, 605
Sse9I AATT 5 cut(s) 549, 619, 713, 827, 1059
SsiI CCGC 5 cut(s) 72, 92, 628, 677, 718
SspMI CTAG 2 cut(s) 53, 879
StyD4I CCNGG 3 cut(s) 817, 930, 1074
TaaI ACNGT 2 cut(s) 130, 641
TaqI TCGA 6 cut(s) 5, 132, 389, 567, 804, 1004
TaqII GACCGA 1 cut(s) 969
TasI AATT 5 cut(s) 549, 619, 713, 827, 1059
TauI GCSGC 2 cut(s) 74, 94
TfiI GAWTC 6 cut(s) 106, 122, 228, 732, 787, 1006
Tru1I TTAA 3 cut(s) 26, 606, 1081
Tru9I TTAA 3 cut(s) 26, 606, 1081
TseI GCWGC 1 cut(s) 68
TspDTI ATGAA 5 cut(s) 25, 520, 568, 934, 998
TspGWI ACGGA 3 cut(s) 225, 237, 252
Vha464I CTTAAG 1 cut(s) 605
XapI RAATTY 4 cut(s) 549, 619, 713, 827
XceI RCATGY 1 cut(s) 427
XhoI CTCGAG 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 2 cut(s) 53, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.