AT2G25070

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000743
Physical Location & Seq
Reverse (-)
10663155 .. 10666022
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25070.1

Sequence Viewer

Length: 1068 bp
ATGGGTACATACCTAAGTTCTCCGAAAACTGAAAAGTTATCAGAAGATGGTGAGAATGATAAGCTCAGATTTGGTTTATCGTCTATGCAAGGTTGGCGCGCTACCATGGAAGACGCGCATGCTGCAATTCTTGATCTTGATGATAAGACATCGTTCTTCGGTGTGTATGATGGCCATGGAGGTAAAGTCGTTGCAAAGTTCTGTGCCAAGTATCTACACCAGCAGGTTATCAGTAATGAAGCGTATAAAACTGGAGACGTCGAAACATCTCTTCGAAGAGCATTCTTTAGAATGGATGACATGATGCAAGGACAAAGAGGATGGCGAGAGTTAGCTGTACTTGGCGACAAGATGAACAAATTTAGCGGCATGATTGAAGGATTTATATGGTCACCAAGAAGCGGTGACACCAATAACCAACCCGATAGTTGGCCTCTTGAAGATGGTCCTCATTCTGATTTCACGGGACCTACCTCGGGGTGCACAGCGTGTGTAGCTCTTATTAAAGATAAGAAGCTCTTTGTTGCAAATGCCGGTGACTCACGTTGTGTGATATCAAGAAAGAGTCAGGCTTACAATCTTTCTAAAGATCACAAGCCTGATCTTGAAGTTGAAAAAGAAAGGATATTGAAAGCTGGTGGCTTTATTCACGCTGGGAGAATCAATGGAAGCTTGAATCTGACAAGAGCCATTGGTGATATGGAGTTCAAGCAGAATAAGTTTTTACCATCTGAAAAGCAAATGGTTACTGCTGATCCAGATATAAACACTATTGACCTATGTGATGATGATGACTTTCTTGTTGTTGCTTGCGATGGAATATGGGATTGTATGTCAAGCCAGGAACTAGTTGATTTTATCCATGAACAGTTAAAATCTGAAACAAAACTTTCAACAGTATGTGAAAAGGTTGTTGATAGATGTTTGGCTCCAGATACAGCGACTGGTGAAGGTTGTGATAATATGACCATCATCTTGGTTCAGTTCAAGAAGCCTAACCCATCTGAGACTGAACCAGAAGATTCCAAACCAGAACCAAGCGAAGATGAGCCGAGCTCATCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

355

Amino Acids

39.35

Weight (kDa)

4.83

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 23 - 103 2.2e-12 Protein phosphatase 2C
PP2C PF00481 157 - 314 6.1e-61 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
Acc36I ACCTGC 1 cut(s) 214
AccII CGCG 2 cut(s) 99, 116
AciI CCGC 2 cut(s) 366, 402
AclWI GGATC 1 cut(s) 749
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 1 cut(s) 359
AcyI GRCGYC 1 cut(s) 258
AdeI CACNNNGTG 2 cut(s) 489, 548
AfaI GTAC 2 cut(s) 7, 339
AfiI CCNNNNNNNGG 3 cut(s) 401, 429, 476
AgsI TTSAA 9 cut(s) 377, 440, 608, 614, 631, 676, 709, 894, 988
AhlI ACTAGT 1 cut(s) 847
AjnI CCWGG 1 cut(s) 840
AluBI AGCT 8 cut(s) 64, 335, 497, 517, 635, 672, 1056, 1065
AluI AGCT 8 cut(s) 64, 335, 497, 517, 635, 672, 1056, 1065
Alw21I GWGCWC 2 cut(s) 485, 1058
Alw26I GTCTC 2 cut(s) 249, 1001
Alw44I GTGCAC 1 cut(s) 481
AlwI GGATC 1 cut(s) 749
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 1 cut(s) 475
AoxI GGCC 2 cut(s) 172, 431
ApaLI GTGCAC 1 cut(s) 481
ApeKI GCWGC 1 cut(s) 122
ApoI RAATTY 1 cut(s) 359
AspLEI GCGC 3 cut(s) 99, 101, 118
AspS9I GGNCC 2 cut(s) 446, 467
AsuHPI GGTGA 6 cut(s) 62, 384, 416, 548, 707, 959
AsuII TTCGAA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 475
AvaII GGWCC 2 cut(s) 446, 467
BaeGI GKGCMC 1 cut(s) 485
BalI TGGCCA 1 cut(s) 174
BanII GRGCYC 1 cut(s) 1058
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 485, 1058
BbvI GCAGC 1 cut(s) 109
BccI CCATC 8 cut(s) 41, 164, 315, 437, 736, 809, 977, 1009
BciT130I CCWGG 1 cut(s) 842
BcoDI GTCTC 2 cut(s) 249, 1001
BcuI ACTAGT 1 cut(s) 847
BfaI CTAG 2 cut(s) 848, 1066
BfuAI ACCTGC 1 cut(s) 214
BisI GCNGC 2 cut(s) 123, 367
BlsI GCNGC 2 cut(s) 124, 368
Bme1390I CCNGG 1 cut(s) 842
Bme18I GGWCC 2 cut(s) 446, 467
BmeT110I CYCGRG 1 cut(s) 475
BmgT120I GGNCC 2 cut(s) 446, 467
BmiI GGNNCC 2 cut(s) 468, 930
BmrFI CCNGG 1 cut(s) 842
BmsI GCATC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 1 cut(s) 1040
BpmI CTGGAG 2 cut(s) 273, 915
Bpu14I TTCGAA 1 cut(s) 274
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 3 cut(s) 105, 175, 474
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 429, 476
Bse118I RCCGGY 1 cut(s) 533
Bse1I ACTGG 2 cut(s) 256, 949
BseBI CCWGG 1 cut(s) 842
BseDI CCNNGG 3 cut(s) 105, 175, 474
BseGI GGATG 2 cut(s) 301, 326
BseLI CCNNNNNNNGG 3 cut(s) 401, 429, 476
BseMII CTCAG 2 cut(s) 79, 996
BseNI ACTGG 2 cut(s) 256, 949
BsePI GCGCGC 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 1 cut(s) 109
BseYI CCCAGC 1 cut(s) 653
Bsh1236I CGCG 2 cut(s) 99, 116
BshFI GGCC 2 cut(s) 174, 433
BsiHKAI GWGCWC 2 cut(s) 485, 1058
BsiHKCI CYCGRG 1 cut(s) 475
BsiSI CCGG 1 cut(s) 534
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 3 cut(s) 401, 429, 476
BsmAI GTCTC 2 cut(s) 249, 1001
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 480
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 2 cut(s) 174, 433
BsoBI CYCGRG 1 cut(s) 475
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 485, 1058
Bsp143I GATC 4 cut(s) 133, 589, 601, 754
Bsp19I CCATGG 2 cut(s) 105, 175
BspACI CCGC 2 cut(s) 366, 402
BspANI GGCC 2 cut(s) 174, 433
BspCNI CTCAG 2 cut(s) 78, 997
BspFNI CGCG 2 cut(s) 99, 116
BspLI GGNNCC 2 cut(s) 468, 930
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 1 cut(s) 749
BspQI GCTCTTC 1 cut(s) 271
BspT104I TTCGAA 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 533
BsrI ACTGG 2 cut(s) 256, 949
BssAI RCCGGY 1 cut(s) 533
BssECI CCNNGG 3 cut(s) 105, 175, 474
BssHII GCGCGC 1 cut(s) 97
BssMI GATC 4 cut(s) 133, 589, 601, 754
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 2 cut(s) 105, 175
Bst2UI CCWGG 1 cut(s) 842
Bst4CI ACNGT 2 cut(s) 870, 898
Bst6I CTCTTC 2 cut(s) 271, 276
BstACI GRCGYC 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 3 cut(s) 99, 120, 811
BstDEI CTNAG 3 cut(s) 14, 65, 1005
BstDSI CCRYGG 2 cut(s) 105, 175
BstEII GGTNACC 1 cut(s) 390
BstF5I GGATG 2 cut(s) 301, 326
BstFNI CGCG 2 cut(s) 99, 116
BstHHI GCGC 3 cut(s) 99, 101, 118
BstKTI GATC 4 cut(s) 136, 592, 604, 757
BstMAI GTCTC 2 cut(s) 249, 1001
BstMBI GATC 4 cut(s) 133, 589, 601, 754
BstMWI GCNNNNNNNGC 4 cut(s) 94, 122, 494, 1062
BstNI CCWGG 1 cut(s) 842
BstNSI RCATGY 1 cut(s) 122
BstPI GGTNACC 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 840
BstSLI GKGCMC 1 cut(s) 485
BstUI CGCG 2 cut(s) 99, 116
BstV1I GCAGC 1 cut(s) 109
BstV2I GAAGAC 1 cut(s) 117
BstXI CCANNNNNNTGG 1 cut(s) 976
BsuRI GGCC 2 cut(s) 174, 433
BtgI CCRYGG 2 cut(s) 105, 175
BtgZI GCGATG 1 cut(s) 828
BtsCI GGATG 2 cut(s) 301, 326
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 3 cut(s) 99, 120, 811
CaiI CAGNNNCTG 1 cut(s) 944
CfoI GCGC 3 cut(s) 99, 101, 118
Cfr10I RCCGGY 1 cut(s) 533
Cfr13I GGNCC 2 cut(s) 446, 467
CseI GACGC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 6, 338
CviAII CATG 6 cut(s) 106, 119, 176, 301, 370, 863
CviQI GTAC 2 cut(s) 6, 338
DdeI CTNAG 3 cut(s) 14, 65, 1005
DpnI GATC 4 cut(s) 135, 591, 603, 756
DpnII GATC 4 cut(s) 133, 589, 601, 754
DraIII CACNNNGTG 2 cut(s) 489, 548
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 2 cut(s) 271, 276
EarI CTCTTC 2 cut(s) 271, 276
Ecl136II GAGCTC 1 cut(s) 1056
Eco130I CCWWGG 2 cut(s) 105, 175
Eco24I GRGCYC 1 cut(s) 1058
Eco32I GATATC 1 cut(s) 555
Eco47I GGWCC 2 cut(s) 446, 467
Eco53kI GAGCTC 1 cut(s) 1056
Eco88I CYCGRG 1 cut(s) 475
Eco91I GGTNACC 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 1056
EcoO109I RGGNCCY 1 cut(s) 467
EcoO65I GGTNACC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 840
EcoRV GATATC 1 cut(s) 555
EcoT14I CCWWGG 2 cut(s) 105, 175
EcoT38I GRGCYC 1 cut(s) 1058
ErhI CCWWGG 2 cut(s) 105, 175
Esp3I CGTCTC 1 cut(s) 249
FaeI CATG 6 cut(s) 109, 122, 179, 304, 373, 866
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 1 cut(s) 480
FatI CATG 6 cut(s) 105, 118, 175, 300, 369, 862
Fnu4HI GCNGC 2 cut(s) 123, 367
FokI GGATG 2 cut(s) 308, 333
FriOI GRGCYC 1 cut(s) 1058
Fsp4HI GCNGC 2 cut(s) 123, 367
FspBI CTAG 2 cut(s) 848, 1066
GlaI GCGC 3 cut(s) 98, 100, 117
GluI GCNGC 2 cut(s) 123, 367
GsaI CCCAGC 1 cut(s) 657
GsuI CTGGAG 2 cut(s) 273, 915
HaeIII GGCC 2 cut(s) 174, 433
HapII CCGG 1 cut(s) 534
HgaI GACGC 1 cut(s) 122
HhaI GCGC 3 cut(s) 99, 101, 118
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 6 cut(s) 109, 122, 179, 304, 373, 866
Hin6I GCGC 3 cut(s) 97, 99, 116
HinP1I GCGC 3 cut(s) 97, 99, 116
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 5 cut(s) 539, 565, 660, 676, 1022
HpaII CCGG 1 cut(s) 534
HphI GGTGA 6 cut(s) 62, 384, 416, 548, 707, 959
Hpy166II GTNNAC 1 cut(s) 483
Hpy188I TCNGA 8 cut(s) 24, 43, 68, 457, 681, 733, 880, 1006
Hpy188III TCNNGA 8 cut(s) 131, 137, 437, 558, 605, 758, 932, 988
Hpy8I GTNNAC 1 cut(s) 483
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 371, 944
HpyCH4III ACNGT 2 cut(s) 870, 898
HpyCH4IV ACGT 2 cut(s) 258, 544
HpyCH4V TGCA 6 cut(s) 88, 125, 194, 307, 483, 527
HpyF10VI GCNNNNNNNGC 4 cut(s) 94, 122, 494, 1062
HpyF3I CTNAG 3 cut(s) 14, 65, 1005
HpySE526I ACGT 2 cut(s) 258, 544
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 6 cut(s) 109, 122, 179, 304, 373, 866
HspAI GCGC 3 cut(s) 97, 99, 116
Kzo9I GATC 4 cut(s) 133, 589, 601, 754
LguI GCTCTTC 1 cut(s) 271
LmnI GCTCC 1 cut(s) 934
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 294
MaeI CTAG 2 cut(s) 848, 1066
MaeII ACGT 2 cut(s) 258, 544
MaeIII GTNAC 4 cut(s) 390, 404, 536, 745
MalI GATC 4 cut(s) 135, 591, 603, 756
MboI GATC 4 cut(s) 133, 589, 601, 754
MboII GAAGA 8 cut(s) 56, 122, 148, 263, 288, 452, 1031, 1055
MhlI GDGCHC 2 cut(s) 485, 1058
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 2 cut(s) 126, 359
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 2 cut(s) 533, 574
MnlI CCTC 5 cut(s) 173, 311, 444, 459, 484
Mox20I TGGCCA 1 cut(s) 174
MscI TGGCCA 1 cut(s) 174
MseI TTAA 2 cut(s) 504, 872
Msp20I TGGCCA 1 cut(s) 174
MspI CCGG 1 cut(s) 534
MspR9I CCNGG 1 cut(s) 842
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 1 cut(s) 842
MvnI CGCG 2 cut(s) 99, 116
MwoI GCNNNNNNNGC 4 cut(s) 94, 122, 494, 1062
NcoI CCATGG 2 cut(s) 105, 175
NdeII GATC 4 cut(s) 133, 589, 601, 754
NlaIII CATG 6 cut(s) 109, 122, 179, 304, 373, 866
NlaIV GGNNCC 2 cut(s) 468, 930
NmuCI GTSAC 3 cut(s) 390, 404, 536
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 1 cut(s) 274
PaeI GCATGC 1 cut(s) 122
PauI GCGCGC 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 271
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 3 cut(s) 660, 676, 1022
PkrI GCNGC 2 cut(s) 124, 368
PleI GAGTC 2 cut(s) 533, 573
PpsI GAGTC 2 cut(s) 533, 573
PpuMI RGGWCCY 1 cut(s) 467
Psp124BI GAGCTC 1 cut(s) 1058
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 840
PspEI GGTNACC 1 cut(s) 390
PspFI CCCAGC 1 cut(s) 653
PspGI CCWGG 1 cut(s) 840
PspN4I GGNNCC 2 cut(s) 468, 930
PspPI GGNCC 2 cut(s) 446, 467
PspPPI RGGWCCY 1 cut(s) 467
PstNI CAGNNNCTG 1 cut(s) 944
PteI GCGCGC 1 cut(s) 97
RsaI GTAC 2 cut(s) 7, 339
RsaNI GTAC 2 cut(s) 6, 338
SacI GAGCTC 1 cut(s) 1058
SapI GCTCTTC 1 cut(s) 271
SaqAI TTAA 2 cut(s) 504, 872
SatI GCNGC 2 cut(s) 123, 367
Sau3AI GATC 4 cut(s) 133, 589, 601, 754
Sau96I GGNCC 2 cut(s) 446, 467
SchI GAGTC 2 cut(s) 533, 574
ScrFI CCNGG 1 cut(s) 842
SduI GDGCHC 2 cut(s) 485, 1058
SfaNI GCATC 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 2 cut(s) 446, 467
SpeI ACTAGT 1 cut(s) 847
SphI GCATGC 1 cut(s) 122
Sse9I AATT 2 cut(s) 126, 359
SsiI CCGC 2 cut(s) 366, 402
SspMI CTAG 2 cut(s) 848, 1066
SstI GAGCTC 1 cut(s) 1058
StyD4I CCNGG 1 cut(s) 840
StyI CCWWGG 2 cut(s) 105, 175
TaaI ACNGT 2 cut(s) 870, 898
TaiI ACGT 2 cut(s) 261, 547
TaqI TCGA 2 cut(s) 261, 274
TasI AATT 2 cut(s) 126, 359
TatI WGTACW 1 cut(s) 337
TauI GCSGC 1 cut(s) 369
TfiI GAWTC 3 cut(s) 660, 676, 1022
Tru1I TTAA 2 cut(s) 504, 872
Tru9I TTAA 2 cut(s) 504, 872
TseFI GTSAC 3 cut(s) 390, 404, 536
TseI GCWGC 1 cut(s) 122
Tsp45I GTSAC 3 cut(s) 390, 404, 536
TspDTI ATGAA 3 cut(s) 252, 368, 879
VneI GTGCAC 1 cut(s) 481
VpaK11BI GGWCC 2 cut(s) 446, 467
XapI RAATTY 1 cut(s) 359
XceI RCATGY 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 697
XspI CTAG 2 cut(s) 848, 1066
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.