RchiOBHm_Chr2g0132691

protein phosphatase 2C 60

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000743
Physical Location & Seq
Forward (+)
49288398 .. 49296316
7919 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50393

Sequence Viewer

Length: 1095 bp
ATGGGAATATATCTGAGCGCTCCGAAAACCGATAAGGTGTCAGAAGATGGTGAGAATGAGAGAGTGCGATTTGGGGCTTCCTCCATGCAGGGGTGGCGTTCAACCATGGAAGATGCTCATGCTGCTTTTCCAGATCTCGACAATTCAACATCTTTCTTTGGTGTTTATGATGGTCATGGAGGTAAAGCGGTGGCAAATTTCTGTGCCAAGTATCTCCACTTACAAGTGCTCAACCAGGAAGCATATCTAGCTGGTGATTTAGGCACTTCCCTGCAGAAAGCTTTTCTCAGGATGGATGAGATGATGCGTGGACAGAGAGGATGGAGAGAACTAGCTGTATTGGGAGATAAGATAGACAAAGTTTCAGGCCTTATTGAAGGGTTCATCTTTTCTCCTAAGAGTGTTGAAGCTAAGAGAAGTGCATTCAATGATCAAATTGATCAGTGGTCTTCTGAGGAGGGGCCTCACTCTGATTTTGATGGACCAAACTCTGGAAGCACAGCTTGTGTTGCTATCATCCGAAACAATCAACTTGTTGTTGCCAATGCTGGTGATTCGCGTTGTGTAATATCGAGGAAGGGTCAGGCATATCCTTTGTCTAAAGATCATAAGCCTGACATTGAGAATGAGAAGGATAGAATTCTGAAGGCCGGTGGATTTATCCAAGTTGGACGGGTCAATGGAAGTTTGAACTTGGCAAGAGCGATTGGAGATGTGGAATTCAAGCAAAACAAACAATTGCCTGTTGAAAAGCAGATTGTAACTGCCAATCCAGATGTAACAAGTGTTGAGATTTGTGATGATGATGAGTTTCTTGTCATCGCTTGTGATGGGATATGGGACTGCATGTCAAACCAACAACTTGTGGATTATGTTGGGGAACAATTGAAACATGAACGTAAACTCTCGGTGGTTTGTGAGAGAGTGTTTGAAAGGTGCTTGGCACCCTCGACAGGTGGTGAAGGCTGTGACAACATGACAATGATCTTGGTTCAGTTTAAGAAGCCTATTATTTCAACTGCTTCTACTGGGAACAAGCCTCTTTCGTCTAATCCATCACTTGAGAAGGACAAGAGCTCGGGAAAAGCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

364

Amino Acids

39.84

Weight (kDa)

5.33

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 23 - 104 3.8e-12 Protein phosphatase 2C
PP2C PF00481 135 - 318 1.9e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 943
AccB7I CCANNNNNTGG 1 cut(s) 491
AccII CGCG 1 cut(s) 559
AciI CCGC 1 cut(s) 188
AcsI RAATTY 3 cut(s) 196, 639, 719
AcuI CTGAAG 1 cut(s) 665
AfeI AGCGCT 1 cut(s) 19
AfiI CCNNNNNNNGG 3 cut(s) 90, 491, 953
AhdI GACNNNNNGTC 1 cut(s) 847
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 7 cut(s) 251, 281, 335, 410, 503, 1077, 1088
AluI AGCT 7 cut(s) 251, 281, 335, 410, 503, 1077, 1088
Alw21I GWGCWC 2 cut(s) 231, 1079
Ama87I CYCGRG 1 cut(s) 1078
Aor51HI AGCGCT 1 cut(s) 19
AoxI GGCC 3 cut(s) 367, 461, 648
ApeKI GCWGC 1 cut(s) 122
ApoI RAATTY 3 cut(s) 196, 639, 719
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 2 cut(s) 461, 482
AsuHPI GGTGA 4 cut(s) 62, 266, 563, 971
AvaI CYCGRG 1 cut(s) 1078
AvaII GGWCC 1 cut(s) 482
BanI GGYRCC 1 cut(s) 943
BanII GRGCYC 1 cut(s) 1079
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 231, 1079
BbvI GCAGC 1 cut(s) 109
BccI CCATC 7 cut(s) 41, 164, 286, 315, 473, 824, 1063
BciT130I CCWGG 1 cut(s) 236
BclI TGATCA 2 cut(s) 430, 439
BfaI CTAG 2 cut(s) 248, 332
BfmI CTRYAG 1 cut(s) 272
BfoI RGCGCY 1 cut(s) 21
BglII AGATCT 1 cut(s) 133
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 1 cut(s) 482
BmeRI GACNNNNNGTC 1 cut(s) 847
BmeT110I CYCGRG 1 cut(s) 1078
BmgT120I GGNCC 2 cut(s) 461, 482
BmiI GGNNCC 2 cut(s) 462, 945
BmrFI CCNGG 1 cut(s) 236
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 2 cut(s) 103, 294
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 1082
BsaJI CCNNGG 1 cut(s) 105
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 491, 953
Bse118I RCCGGY 1 cut(s) 650
Bse1I ACTGG 1 cut(s) 1033
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 1 cut(s) 105
BseGI GGATG 4 cut(s) 297, 301, 326, 516
BseLI CCNNNNNNNGG 3 cut(s) 90, 491, 953
BseMII CTCAG 3 cut(s) 5, 301, 444
BseNI ACTGG 1 cut(s) 1033
BseRI GAGGAG 1 cut(s) 470
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 1 cut(s) 559
BshFI GGCC 3 cut(s) 369, 463, 650
BshNI GGYRCC 1 cut(s) 943
BsiHKAI GWGCWC 2 cut(s) 231, 1079
BsiHKCI CYCGRG 1 cut(s) 1078
BsiSI CCGG 1 cut(s) 651
BslFI GGGAC 1 cut(s) 854
BslI CCNNNNNNNGG 3 cut(s) 90, 491, 953
BsmFI GGGAC 1 cut(s) 854
BsmI GAATGC 1 cut(s) 422
BsnI GGCC 3 cut(s) 369, 463, 650
BsoBI CYCGRG 1 cut(s) 1078
Bsp1286I GDGCHC 2 cut(s) 231, 1079
Bsp143I GATC 5 cut(s) 133, 430, 439, 604, 984
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 1 cut(s) 188
BspANI GGCC 3 cut(s) 369, 463, 650
BspCNI CTCAG 3 cut(s) 6, 300, 445
BspFNI CGCG 1 cut(s) 559
BspLI GGNNCC 2 cut(s) 462, 945
BspMAI CTGCAG 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 943
BsrFI RCCGGY 1 cut(s) 650
BsrI ACTGG 1 cut(s) 1033
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 5 cut(s) 133, 430, 439, 604, 984
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 236
BstDEI CTNAG 5 cut(s) 14, 287, 396, 411, 453
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 4 cut(s) 297, 301, 326, 516
BstFNI CGCG 1 cut(s) 559
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 5 cut(s) 136, 433, 442, 607, 987
BstMBI GATC 5 cut(s) 133, 430, 439, 604, 984
BstMWI GCNNNNNNNGC 4 cut(s) 94, 122, 248, 509
BstNI CCWGG 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 850
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 272
BstUI CGCG 1 cut(s) 559
BstV1I GCAGC 1 cut(s) 109
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 3 cut(s) 369, 463, 650
BtgI CCRYGG 1 cut(s) 105
BtgZI GCGATG 1 cut(s) 805
BtsCI GGATG 4 cut(s) 297, 301, 326, 516
BtsIMutI CAGTG 1 cut(s) 449
CfoI GCGC 1 cut(s) 20
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 2 cut(s) 461, 482
CviAII CATG 7 cut(s) 85, 106, 119, 176, 847, 893, 976
DdeI CTNAG 5 cut(s) 14, 287, 396, 411, 453
DpnI GATC 5 cut(s) 135, 432, 441, 606, 986
DpnII GATC 5 cut(s) 133, 430, 439, 604, 984
DriI GACNNNNNGTC 1 cut(s) 847
Eam1105I GACNNNNNGTC 1 cut(s) 847
Ecl136II GAGCTC 1 cut(s) 1077
Eco130I CCWWGG 1 cut(s) 105
Eco147I AGGCCT 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 1079
Eco47I GGWCC 1 cut(s) 482
Eco47III AGCGCT 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 1077
Eco57I CTGAAG 1 cut(s) 665
Eco88I CYCGRG 1 cut(s) 1078
EcoICRI GAGCTC 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 461
EcoRI GAATTC 2 cut(s) 639, 719
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 1079
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 7 cut(s) 88, 109, 122, 179, 850, 896, 979
FalI AAGNNNNNCTT 2 cut(s) 487, 519
FaqI GGGAC 1 cut(s) 854
FatI CATG 7 cut(s) 84, 105, 118, 175, 846, 892, 975
FbaI TGATCA 2 cut(s) 430, 439
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 4 cut(s) 304, 308, 333, 503
FriOI GRGCYC 1 cut(s) 1079
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 2 cut(s) 248, 332
GlaI GCGC 1 cut(s) 19
GluI GCNGC 1 cut(s) 123
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 3 cut(s) 369, 463, 650
HapII CCGG 1 cut(s) 651
HhaI GCGC 1 cut(s) 20
Hin1II CATG 7 cut(s) 88, 109, 122, 179, 850, 896, 979
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 1 cut(s) 554
HpaII CCGG 1 cut(s) 651
HphI GGTGA 4 cut(s) 62, 266, 563, 971
Hpy166II GTNNAC 2 cut(s) 311, 902
Hpy188I TCNGA 7 cut(s) 15, 24, 43, 454, 472, 521, 645
Hpy188III TCNNGA 6 cut(s) 131, 137, 289, 492, 773, 1080
Hpy8I GTNNAC 2 cut(s) 311, 902
HpyAV CCTTC 6 cut(s) 371, 571, 625, 640, 956, 1060
HpyCH4IV ACGT 1 cut(s) 898
HpyCH4V TGCA 4 cut(s) 88, 274, 422, 846
HpyF10VI GCNNNNNNNGC 4 cut(s) 94, 122, 248, 509
HpyF3I CTNAG 5 cut(s) 14, 287, 396, 411, 453
HpySE526I ACGT 1 cut(s) 898
Hsp92II CATG 7 cut(s) 88, 109, 122, 179, 850, 896, 979
HspAI GCGC 1 cut(s) 18
Ksp22I TGATCA 2 cut(s) 430, 439
Kzo9I GATC 5 cut(s) 133, 430, 439, 604, 984
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 2 cut(s) 103, 294
MaeI CTAG 2 cut(s) 248, 332
MaeII ACGT 1 cut(s) 898
MaeIII GTNAC 3 cut(s) 760, 778, 968
MalI GATC 5 cut(s) 135, 432, 441, 606, 986
MboI GATC 5 cut(s) 133, 430, 439, 604, 984
MboII GAAGA 3 cut(s) 56, 122, 441
MfeI CAATTG 2 cut(s) 737, 884
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 2 cut(s) 231, 1079
MluCI AATT 7 cut(s) 142, 196, 435, 639, 719, 737, 884
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 9 cut(s) 91, 173, 311, 448, 451, 474, 567, 958, 1050
MseI TTAA 1 cut(s) 999
MslI CAYNNNNRTG 1 cut(s) 980
MspI CCGG 1 cut(s) 651
MspR9I CCNGG 1 cut(s) 236
MunI CAATTG 2 cut(s) 737, 884
Mva1269I GAATGC 1 cut(s) 422
MvaI CCWGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 559
MwoI GCNNNNNNNGC 4 cut(s) 94, 122, 248, 509
NcoI CCATGG 1 cut(s) 105
NdeII GATC 5 cut(s) 133, 430, 439, 604, 984
NlaIII CATG 7 cut(s) 88, 109, 122, 179, 850, 896, 979
NlaIV GGNNCC 2 cut(s) 462, 945
NmuCI GTSAC 1 cut(s) 968
NspI RCATGY 1 cut(s) 850
PceI AGGCCT 1 cut(s) 369
PctI GAATGC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 554
PflMI CCANNNNNTGG 1 cut(s) 491
PkrI GCNGC 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 1079
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 462, 945
PspPI GGNCC 2 cut(s) 461, 482
PstI CTGCAG 1 cut(s) 276
PsuI RGATCY 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 980
SacI GAGCTC 1 cut(s) 1079
SaqAI TTAA 1 cut(s) 999
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 5 cut(s) 133, 430, 439, 604, 984
Sau96I GGNCC 2 cut(s) 461, 482
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 2 cut(s) 231, 1079
SfaNI GCATC 2 cut(s) 103, 294
SfcI CTRYAG 1 cut(s) 272
SinI GGWCC 1 cut(s) 482
SmiMI CAYNNNNRTG 1 cut(s) 980
SmlI CTYRAG 1 cut(s) 1061
SmoI CTYRAG 1 cut(s) 1061
Sse9I AATT 7 cut(s) 142, 196, 435, 639, 719, 737, 884
SseBI AGGCCT 1 cut(s) 369
SsiI CCGC 1 cut(s) 188
SspMI CTAG 2 cut(s) 248, 332
SstI GAGCTC 1 cut(s) 1079
StuI AGGCCT 1 cut(s) 369
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 1 cut(s) 105
TaiI ACGT 1 cut(s) 901
TaqI TCGA 3 cut(s) 138, 572, 950
TasI AATT 7 cut(s) 142, 196, 435, 639, 719, 737, 884
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 1 cut(s) 999
Tru9I TTAA 1 cut(s) 999
TscAI CASTG 1 cut(s) 449
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 1 cut(s) 122
Tsp45I GTSAC 1 cut(s) 968
TspDTI ATGAA 2 cut(s) 373, 909
TspRI CASTG 1 cut(s) 449
Van91I CCANNNNNTGG 1 cut(s) 491
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 3 cut(s) 196, 639, 719
XceI RCATGY 1 cut(s) 850
XspI CTAG 2 cut(s) 248, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.