Rorug02G0306100

protein phosphatase 2C 60

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000743
Physical Location & Seq
Reverse (-)
34628665 .. 34629855
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0306100.1

Sequence Viewer

Length: 1191 bp
ATGGGGAAGAACGACTTCCTCACACCCAAGGCAATCGCGAACCGAATCAAAGCCAAGGGGCTGCAGAAGCTCCGGTGGTACTGCCAGATGTGCCAGAAGCAATGCCGTGACGAAAACGGTTTCAAGTGCCACTGCATGAGCGAGAGCCACCAGCGCCAGATGCTCATCTTCGGCGAGAACCCCAACCGCGTCGTCGAGGGCTACTCTGAGGAGTTCGAGACCAGTTTCTTGGAGCACATGAAGCGCAGCCACCGCTTCAGCCGCATCGCCGCCACCGTCATTTACAACGAGTACATCAATGACCGCCACCACGTGCACATGAACTCGACGGAGTGGGCCACGCTGACGGAGTTTGTGAAGCATTTGGGGAGGACCGGCAAGTGTAAGGTGGAGGAGACACCGAAGGGGTGGTTCATTACTTATATTGATAGGGATTCGGAGACTCTGTTTAAGGAGAGGGAGAAGATGAAGAGAGTGAAGGCCGATTTGGCGGAGGAGGAGAAGCAGGAGAGGGAGATTAAGAAGCAGATTGAGAGGGTTTATCAGTCAATGCCGAATGCAGATAATGTAGAGGGTCACGGTGGTGAGGAGGTGAGTAGGGAGTTGAAAGTGGAGAGTGGTGTGAAAATCGGGTTTGCGCTTGGGGGATTGAAGAAAGAGAAGGGGGAGAAAGGGGAGAGTTTGAAATTGGTGTTTGAGGAGGTGGAGGATGAGAGGAAGAGTAAGAAGTCGAAAACCGGGGGTGGTAATGGTAGTGGTACTGGTGGTGCAGGGGGTTTGGCATTGGAGGAGTTGATGAGGGAAGAGGAGAGGAAGAAAGAGAGGGTTAATAGGAAGGACTATTGGGTGTGTGAGGGGATCATTGTGAAAGTTATGAGTAAAGCTTTGGCGGAGAAGGGTTACTATAAGCAAAAAGGGCTTGTGAAGAAAGTAATTGACAAGTATGTTGGGGAGATTGAAATGCTTGAAAGCAAACATGTCTTGAGAGTTGATCAGCAGGAGCTTGAGACGGTGATTCCTCAAATTGGGTGCCTTGTGAAGATAGTGAATGGCGCGTACAGAGGATCGAATGCGAGGTTGCTGGCAGTGGACACGGATAAGTTTTGCGCTAAGGTGCAGATAGAGAAGGGCGTCTATGATGGTAGGGTGCTTAAAGCTGTTGAGTATGAGGATATTTGTAAACTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

396

Amino Acids

45.4

Weight (kDa)

8.76

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-zf_KIN17 PF25095 24 - 53 2.6e-20 KIN17 C2H2-type zinc finger domain
WH_KIN17 PF10357 59 - 177 1.6e-45 KIN17 WH-like domain
KN17_SH3 PF18131 278 - 331 9.5e-23 KN17 SH3-like domain
GPKOW_C PF25088 287 - 394 2.9e-16 GPKOW C-terminal domain
SH3_KIN17_C PF25092 340 - 396 9.3e-23 KIN17 C-terminal SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1029
AccII CGCG 3 cut(s) 38, 189, 1055
AciI CCGC 7 cut(s) 187, 253, 262, 270, 304, 491, 890
AclWI GGATC 2 cut(s) 866, 1072
AcuI CTGAAG 1 cut(s) 241
AcvI CACGTG 1 cut(s) 313
AcyI GRCGYC 1 cut(s) 1131
AdeI CACNNNGTG 1 cut(s) 313
AfaI GTAC 4 cut(s) 80, 293, 760, 1058
AfiI CCNNNNNNNGG 1 cut(s) 1025
AflIII ACRYGT 1 cut(s) 976
AgsI TTSAA 6 cut(s) 124, 607, 652, 685, 959, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AleI CACNNNNGTG 1 cut(s) 582
AluBI AGCT 4 cut(s) 70, 884, 1003, 1157
AluI AGCT 4 cut(s) 70, 884, 1003, 1157
Alw21I GWGCWC 2 cut(s) 237, 318
Alw26I GTCTC 4 cut(s) 212, 389, 434, 1001
Alw44I GTGCAC 1 cut(s) 314
AlwI GGATC 2 cut(s) 866, 1072
AoxI GGCC 2 cut(s) 336, 480
ApaLI GTGCAC 1 cut(s) 314
ApeKI GCWGC 2 cut(s) 61, 246
Asp700I GAANNNNTTC 1 cut(s) 14
AspLEI GCGC 5 cut(s) 156, 246, 640, 1055, 1109
AspS9I GGNCC 2 cut(s) 336, 372
AsuC2I CCSGG 1 cut(s) 739
AsuHPI GGTGA 3 cut(s) 596, 604, 1024
AvaII GGWCC 1 cut(s) 372
BaeGI GKGCMC 1 cut(s) 318
BanI GGYRCC 1 cut(s) 1029
BbrPI CACGTG 1 cut(s) 313
Bbv12I GWGCWC 2 cut(s) 237, 318
BbvI GCAGC 2 cut(s) 48, 258
BccI CCATC 1 cut(s) 1133
BceAI ACGGC 1 cut(s) 90
BclI TGATCA 1 cut(s) 991
BcnI CCSGG 1 cut(s) 739
BcoDI GTCTC 4 cut(s) 212, 389, 434, 1001
BfmI CTRYAG 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 488
BisI GCNGC 4 cut(s) 62, 247, 262, 270
BlsI GCNGC 4 cut(s) 63, 248, 263, 271
Bme1390I CCNGG 1 cut(s) 739
Bme18I GGWCC 1 cut(s) 372
BmgT120I GGNCC 2 cut(s) 336, 372
BmiI GGNNCC 1 cut(s) 1031
BmrFI CCNGG 1 cut(s) 739
BmsI GCATC 2 cut(s) 150, 273
BplI GAGNNNNNCTC 2 cut(s) 188, 220
Bpu10I CCTNAGC 1 cut(s) 1110
BpuEI CTTGAG 2 cut(s) 1003, 1025
BpuMI CCSGG 1 cut(s) 739
BsaAI YACGTR 1 cut(s) 313
BsaBI GATNNNNATC 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 1131
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 3 cut(s) 27, 54, 738
BsaWI WCCGGW 1 cut(s) 72
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 1 cut(s) 1025
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 2 cut(s) 222, 766
Bse3DI GCAATG 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 164
BseDI CCNNGG 3 cut(s) 27, 54, 738
BseGI GGATG 1 cut(s) 715
BseJI GATNNNNATC 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 1025
BseMI GCAATG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 2 cut(s) 222, 766
BseRI GAGGAG 8 cut(s) 224, 407, 509, 512, 602, 713, 803, 821
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 2 cut(s) 48, 258
BsgI GTGCAG 2 cut(s) 789, 1136
Bsh1236I CGCG 3 cut(s) 38, 189, 1055
BshFI GGCC 2 cut(s) 338, 482
BshNI GGYRCC 1 cut(s) 1029
BsiHKAI GWGCWC 2 cut(s) 237, 318
BsiSI CCGG 3 cut(s) 73, 375, 738
BslI CCNNNNNNNGG 1 cut(s) 1025
BsmAI GTCTC 4 cut(s) 212, 389, 434, 1001
BsmBI CGTCTC 1 cut(s) 1001
BsmI GAATGC 2 cut(s) 562, 1075
BsnI GGCC 2 cut(s) 338, 482
Bso31I GGTCTC 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 237, 318
Bsp143I GATC 3 cut(s) 858, 991, 1064
Bsp68I TCGCGA 1 cut(s) 38
BspACI CCGC 7 cut(s) 187, 253, 262, 270, 304, 491, 890
BspANI GGCC 2 cut(s) 338, 482
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 3 cut(s) 38, 189, 1055
BspLI GGNNCC 1 cut(s) 1031
BspMAI CTGCAG 1 cut(s) 66
BspPI GGATC 2 cut(s) 866, 1072
BspT107I GGYRCC 1 cut(s) 1029
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 107
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 2 cut(s) 222, 766
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 27, 54, 738
BssMI GATC 3 cut(s) 858, 991, 1064
BssNI GRCGYC 1 cut(s) 1131
BssT1I CCWWGG 2 cut(s) 27, 54
Bst4CI ACNGT 4 cut(s) 119, 277, 581, 1012
Bst6I CTCTTC 3 cut(s) 464, 713, 798
BstACI GRCGYC 1 cut(s) 1131
BstBAI YACGTR 1 cut(s) 313
BstC8I GCNNGC 1 cut(s) 1083
BstDEI CTNAG 2 cut(s) 207, 1110
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 3 cut(s) 38, 189, 1055
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 5 cut(s) 156, 246, 640, 1055, 1109
BstKTI GATC 3 cut(s) 861, 994, 1067
BstMAI GTCTC 4 cut(s) 212, 389, 434, 1001
BstMBI GATC 3 cut(s) 858, 991, 1064
BstMWI GCNNNNNNNGC 9 cut(s) 67, 90, 153, 160, 241, 252, 261, 488, 916
BstNSI RCATGY 1 cut(s) 980
BstSCI CCNGG 1 cut(s) 737
BstSFI CTRYAG 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 3 cut(s) 38, 189, 1055
BstV1I GCAGC 2 cut(s) 48, 258
BstXI CCANNNNNNTGG 1 cut(s) 229
BsuRI GGCC 2 cut(s) 338, 482
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 715
BtsI GCAGTG 2 cut(s) 130, 1092
BtsIMutI CAGTG 2 cut(s) 130, 1092
BtuMI TCGCGA 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 1083
CfoI GCGC 5 cut(s) 156, 246, 640, 1055, 1109
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 2 cut(s) 336, 372
CseI GACGC 2 cut(s) 178, 1120
Csp6I GTAC 4 cut(s) 79, 292, 759, 1057
CviAII CATG 4 cut(s) 136, 238, 319, 977
CviQI GTAC 4 cut(s) 79, 292, 759, 1057
DdeI CTNAG 2 cut(s) 207, 1110
DpnI GATC 3 cut(s) 860, 993, 1066
DpnII GATC 3 cut(s) 858, 991, 1064
DraIII CACNNNGTG 1 cut(s) 313
Eam1104I CTCTTC 3 cut(s) 464, 713, 798
EarI CTCTTC 3 cut(s) 464, 713, 798
EciI GGCGGA 2 cut(s) 506, 905
Eco130I CCWWGG 2 cut(s) 27, 54
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 241
Eco72I CACGTG 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 27, 54
ErhI CCWWGG 2 cut(s) 27, 54
Esp3I CGTCTC 1 cut(s) 1001
FaeI CATG 4 cut(s) 139, 241, 322, 980
FalI AAGNNNNNCTT 1 cut(s) 31
FatI CATG 4 cut(s) 135, 237, 318, 976
FbaI TGATCA 1 cut(s) 991
Fnu4HI GCNGC 4 cut(s) 62, 247, 262, 270
FokI GGATG 1 cut(s) 722
Fsp4HI GCNGC 4 cut(s) 62, 247, 262, 270
GlaI GCGC 5 cut(s) 155, 245, 639, 1054, 1108
GluI GCNGC 4 cut(s) 62, 247, 262, 270
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 2 cut(s) 338, 482
HapII CCGG 3 cut(s) 73, 375, 738
HgaI GACGC 2 cut(s) 178, 1120
HhaI GCGC 5 cut(s) 156, 246, 640, 1055, 1109
Hin1I GRCGYC 1 cut(s) 1131
Hin1II CATG 4 cut(s) 139, 241, 322, 980
Hin6I GCGC 5 cut(s) 154, 244, 638, 1053, 1107
HinP1I GCGC 5 cut(s) 154, 244, 638, 1053, 1107
HindIII AAGCTT 1 cut(s) 882
HinfI GANTC 4 cut(s) 45, 434, 442, 1015
HpaII CCGG 3 cut(s) 73, 375, 738
HphI GGTGA 3 cut(s) 596, 604, 1024
Hpy166II GTNNAC 3 cut(s) 316, 1090, 1181
Hpy188I TCNGA 2 cut(s) 208, 439
Hpy188III TCNNGA 3 cut(s) 37, 217, 982
Hpy8I GTNNAC 3 cut(s) 316, 1090, 1181
Hpy99I CGWCG 3 cut(s) 194, 197, 331
HpyAV CCTTC 6 cut(s) 397, 472, 655, 829, 889, 1120
HpyCH4III ACNGT 4 cut(s) 119, 277, 581, 1012
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 7 cut(s) 64, 135, 316, 560, 770, 1117, 1187
HpyF10VI GCNNNNNNNGC 9 cut(s) 67, 90, 153, 160, 241, 252, 261, 488, 916
HpyF3I CTNAG 2 cut(s) 207, 1110
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 1131
Hsp92II CATG 4 cut(s) 139, 241, 322, 980
HspAI GCGC 5 cut(s) 154, 244, 638, 1053, 1107
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 3 cut(s) 858, 991, 1064
LmnI GCTCC 3 cut(s) 75, 232, 1000
Lsp1109I GCAGC 2 cut(s) 48, 258
LweI GCATC 2 cut(s) 150, 273
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 3 cut(s) 107, 575, 899
MalI GATC 3 cut(s) 860, 993, 1066
MboI GATC 3 cut(s) 858, 991, 1064
MhlI GDGCHC 2 cut(s) 237, 318
MluCI AATT 3 cut(s) 686, 933, 1023
MlyI GAGTC 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 4 cut(s) 450, 519, 828, 1152
MslI CAYNNNNRTG 1 cut(s) 582
MspI CCGG 3 cut(s) 73, 375, 738
MspR9I CCNGG 1 cut(s) 739
Mva1269I GAATGC 2 cut(s) 562, 1075
MvnI CGCG 3 cut(s) 38, 189, 1055
MwoI GCNNNNNNNGC 9 cut(s) 67, 90, 153, 160, 241, 252, 261, 488, 916
NciI CCSGG 1 cut(s) 739
NdeII GATC 3 cut(s) 858, 991, 1064
NlaIII CATG 4 cut(s) 139, 241, 322, 980
NlaIV GGNNCC 1 cut(s) 1031
NmuCI GTSAC 2 cut(s) 107, 575
NruI TCGCGA 1 cut(s) 38
NspI RCATGY 1 cut(s) 980
OliI CACNNNNGTG 1 cut(s) 582
PciI ACATGT 1 cut(s) 976
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PctI GAATGC 2 cut(s) 562, 1075
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 3 cut(s) 45, 434, 1015
PkrI GCNGC 4 cut(s) 63, 248, 263, 271
PleI GAGTC 1 cut(s) 436
PmaCI CACGTG 1 cut(s) 313
PmlI CACGTG 1 cut(s) 313
PpsI GAGTC 1 cut(s) 436
Ppu21I YACGTR 1 cut(s) 313
PscI ACATGT 1 cut(s) 976
PspCI CACGTG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 1031
PspPI GGNCC 2 cut(s) 336, 372
PstI CTGCAG 1 cut(s) 66
RruI TCGCGA 1 cut(s) 38
RsaI GTAC 4 cut(s) 80, 293, 760, 1058
RsaNI GTAC 4 cut(s) 79, 292, 759, 1057
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 4 cut(s) 450, 519, 828, 1152
SatI GCNGC 4 cut(s) 62, 247, 262, 270
Sau3AI GATC 3 cut(s) 858, 991, 1064
Sau96I GGNCC 2 cut(s) 336, 372
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 1 cut(s) 739
SduI GDGCHC 2 cut(s) 237, 318
SfaNI GCATC 2 cut(s) 150, 273
SfcI CTRYAG 1 cut(s) 62
SinI GGWCC 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 982, 1004
SmoI CTYRAG 2 cut(s) 982, 1004
Sse9I AATT 3 cut(s) 686, 933, 1023
SsiI CCGC 7 cut(s) 187, 253, 262, 270, 304, 491, 890
StyD4I CCNGG 1 cut(s) 737
StyI CCWWGG 2 cut(s) 27, 54
TaaI ACNGT 4 cut(s) 119, 277, 581, 1012
TaiI ACGT 1 cut(s) 315
TaqI TCGA 5 cut(s) 195, 216, 326, 731, 1067
TasI AATT 3 cut(s) 686, 933, 1023
TatI WGTACW 1 cut(s) 291
TauI GCSGC 2 cut(s) 264, 272
TfiI GAWTC 3 cut(s) 45, 434, 1015
Tru1I TTAA 4 cut(s) 450, 519, 828, 1152
Tru9I TTAA 4 cut(s) 450, 519, 828, 1152
TscAI CASTG 2 cut(s) 137, 1092
TseFI GTSAC 2 cut(s) 107, 575
TseI GCWGC 2 cut(s) 61, 246
Tsp45I GTSAC 2 cut(s) 107, 575
TspDTI ATGAA 4 cut(s) 254, 335, 403, 482
TspGWI ACGGA 3 cut(s) 344, 362, 1109
TspRI CASTG 2 cut(s) 137, 1092
VneI GTGCAC 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 372
XceI RCATGY 1 cut(s) 980
XmnI GAANNNNTTC 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.