FvH4_6g33081

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000743
Physical Location & Seq
Forward (+)
26178779 .. 26179324
546 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g33081.t1

Sequence Viewer

Length: 366 bp
ATGGGTCAGGCATATACGCTGTCCAGAGATCACAAACCTGATCTTGAGCTTGAGAAGGGAAGGATATTAAAAGTCTGTGGTTTTATCCATGCAGGACGAGTCAATGGCAGTCTGAATCTTGCAAGAACTTTAGTAAGCTTAGGGAGCAAACTGTCTGTGGTGGCTGAAAGAGTGTTGGATAAGTGTTTGACACCATCGACTACTGGATGTGATAACATGACCATGATCACTGAAGAGATTGTGCTAGTTGAATATGCGGATAAGCAGCATGAATCCTCAACAATTGGAGCACCACCACATGAAACAGATGCACGTTTAACTGAAAGTGAATCAAATTTGGGGACTGCTGCGTTCCTAAATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

122

Amino Acids

13.12

Weight (kDa)

5.68

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 2 - 44 1.1e-08 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 257
AcsI RAATTY 1 cut(s) 334
AcuI CTGAAG 1 cut(s) 252
AgsI TTSAA 1 cut(s) 251
AluBI AGCT 2 cut(s) 49, 138
AluI AGCT 2 cut(s) 49, 138
Alw21I GWGCWC 1 cut(s) 292
ApeKI GCWGC 2 cut(s) 265, 347
ApoI RAATTY 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 2 cut(s) 277, 334
BccI CCATC 1 cut(s) 202
BclI TGATCA 1 cut(s) 225
BfaI CTAG 1 cut(s) 245
BisI GCNGC 2 cut(s) 266, 348
BlsI GCNGC 2 cut(s) 267, 349
BmsI GCATC 1 cut(s) 298
Bpu10I CCTNAGC 1 cut(s) 139
BpuEI CTTGAG 2 cut(s) 65, 71
Bse1I ACTGG 1 cut(s) 208
BseGI GGATG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 208
BseXI GCAGC 2 cut(s) 277, 334
BsiHKAI GWGCWC 1 cut(s) 292
BslFI GGGAC 1 cut(s) 355
BsmFI GGGAC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 3 cut(s) 28, 40, 225
BspACI CCGC 1 cut(s) 257
BsrI ACTGG 1 cut(s) 208
BssMI GATC 3 cut(s) 28, 40, 225
Bst4CI ACNGT 1 cut(s) 153
Bst6I CTCTTC 1 cut(s) 228
BstDEI CTNAG 1 cut(s) 139
BstF5I GGATG 1 cut(s) 212
BstKTI GATC 3 cut(s) 31, 43, 228
BstMBI GATC 3 cut(s) 28, 40, 225
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 277, 334
BtsCI GGATG 1 cut(s) 212
BtsIMutI CAGTG 2 cut(s) 228, 361
CviAII CATG 5 cut(s) 89, 217, 223, 269, 299
CviJI RGCY 3 cut(s) 49, 138, 164
CviKI_1 RGCY 3 cut(s) 49, 138, 164
DdeI CTNAG 1 cut(s) 139
DpnI GATC 3 cut(s) 30, 42, 227
DpnII GATC 3 cut(s) 28, 40, 225
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 252
FaeI CATG 5 cut(s) 92, 220, 226, 272, 302
FaiI YATR 8 cut(s) 13, 15, 90, 218, 224, 255, 270, 300
FaqI GGGAC 1 cut(s) 355
FatI CATG 5 cut(s) 88, 216, 222, 268, 298
FbaI TGATCA 1 cut(s) 225
Fnu4HI GCNGC 2 cut(s) 266, 348
FokI GGATG 1 cut(s) 219
Fsp4HI GCNGC 2 cut(s) 266, 348
FspBI CTAG 1 cut(s) 245
GluI GCNGC 2 cut(s) 266, 348
Hin1II CATG 5 cut(s) 92, 220, 226, 272, 302
HindIII AAGCTT 1 cut(s) 136
HinfI GANTC 4 cut(s) 99, 115, 272, 329
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 2 cut(s) 24, 44
HpyAV CCTTC 2 cut(s) 49, 54
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 92, 122, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 1 cut(s) 139
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 5 cut(s) 92, 220, 226, 272, 302
Ksp22I TGATCA 1 cut(s) 225
Kzo9I GATC 3 cut(s) 28, 40, 225
LmnI GCTCC 2 cut(s) 144, 287
LpnPI CCDG 4 cut(s) 37, 51, 78, 189
Lsp1109I GCAGC 2 cut(s) 277, 334
LweI GCATC 1 cut(s) 298
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 313
MalI GATC 3 cut(s) 30, 42, 227
MboI GATC 3 cut(s) 28, 40, 225
MboII GAAGA 1 cut(s) 245
MfeI CAATTG 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 2 cut(s) 282, 334
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 1 cut(s) 286
MseI TTAA 2 cut(s) 68, 317
MslI CAYNNNNRTG 1 cut(s) 221
MunI CAATTG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 144
NdeII GATC 3 cut(s) 28, 40, 225
NlaIII CATG 5 cut(s) 92, 220, 226, 272, 302
PfeI GAWTC 3 cut(s) 115, 272, 329
PkrI GCNGC 2 cut(s) 267, 349
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 2 cut(s) 68, 317
SatI GCNGC 2 cut(s) 266, 348
Sau3AI GATC 3 cut(s) 28, 40, 225
SchI GAGTC 1 cut(s) 108
SduI GDGCHC 1 cut(s) 292
SetI ASST 4 cut(s) 40, 51, 140, 316
SfaNI GCATC 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 2 cut(s) 44, 50
SmoI CTYRAG 2 cut(s) 44, 50
Sse9I AATT 2 cut(s) 282, 334
SsiI CCGC 1 cut(s) 257
SspMI CTAG 1 cut(s) 245
TaaI ACNGT 1 cut(s) 153
TaiI ACGT 1 cut(s) 316
TaqI TCGA 1 cut(s) 197
TasI AATT 2 cut(s) 282, 334
TfiI GAWTC 3 cut(s) 115, 272, 329
Tru1I TTAA 2 cut(s) 68, 317
Tru9I TTAA 2 cut(s) 68, 317
TscAI CASTG 1 cut(s) 235
TseI GCWGC 2 cut(s) 265, 347
TspDTI ATGAA 2 cut(s) 285, 315
TspRI CASTG 1 cut(s) 235
XapI RAATTY 1 cut(s) 334
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.