Rh6CG413100

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000743
Physical Location & Seq
Forward (+)
59811665 .. 59821689
10025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG413100.1

Sequence Viewer

Length: 1800 bp
ATGGCTTTTCTGAGCTTCAACTCCATTTGTTGCCTGTCGACTTCGCCGGATCTTCAAAAATGGAATAATATTATAAAGACTCAAGTGCTCGAGGGGAACGCCGGGCAGGCAATTCGGTCGTATGTAAAAATGCAAAGACTTGGTCTCTTTGCTGATAACTATACATTTCCGGTTTTGCTGAAAGCAGCAGGTAATGCTTCCTATTTGGGTATAGGGTTAGCACTTCACGGACAGATACTGAAAACTGGGTTTTGTGACCATCTTTTCGTCCAAACTGCTCTGCTGAAAATGTATTCTTCTCTTGGAATCGTAGCGGATGCCCGCAAAGCGTTTGATAAAATGCCTGTGAAAGATGTGGTAGCCTGGAACTCCATGTTATATGTGTATGCTTCTAGTGGGCAGATGGGGAATGCAGTGCAAGTTTACTCTAAGGCAATCGAATTCTTGGACAAAATGAAGATGGGAAATATCGAACCGGATCATCTGAGTGTGACTGCTGCTTTATCTGCCTGTGCTCATTTGGGGTCGCTTGAAACTGGCATAAAAATACATACATATGCCAAACTCTCCGGGCAGGCTTCAAGTCCCCATGTAGCAACAGCACTCATAGATATCTCTGGTTTCCTTGTCTCCGATTTCGATCTTGAGCTCTTCTCTGCGGCCTCGCCTCGCCTCGCCTCGACTCTCATATCATATTGGAATATGGGTATATATCTCAGCACTCCCAAAACTGACAAGTTCTCTGAAGATGGAGAGAATGGCTGGGTCAGATATGGTCTGTCGTCCATGCAAGGATGGCGTGCAACTATGGAGGATGCTCATGCAGCATATCCCGATCTAGATGCATCCACCTCGTTCTTTGGTGTTTATGATGGCCATGGAGGAAAGGTAGTTGCTAAATTCTGTGCCAAATATCTTCACCGACAAATGCTCAAGAATGAAGCATATTCAGCTGGAGACATAGGAACTTCTGTTCAAAAAGCTTTTTTCAGAATGGATGAAATGATGCGTGGACAAAGGGGTTGGAGAGAGTTAGCTGTTCTGGGAGATAAGATAAACAAATTTACTGGCATGATAGAAGGGTTAATCTGGTCTCCGAGGAGCAGTGAGGGTAATGATCAAGCTGATGATTGGGCTTTTGAGGAGGGGCCTCACTCTGATTTCAGTGGTCCAACTTCTGGGAGCACAGCTTGTGTTGCAATTATTAGAAACAACCAACTTGTTGTTGCAAATGCTGGTGATTCTCGTTGTGTGATATCTAGGAAGGGTCAGGCATATAACCTGTCCAGAGATCACAAACCTGAACTTGAGCTTGAGAAGGAAAGGATTTTAAAAGCTGGTGGTTTTATCCATGCAGGACGAGTCAATGGCAGTTTGAATCTTGCAAGAGCTATAGGTGACATGGAATTCAAGCAGAATAAATTCTTGCCAGTGGAAAAGCAAATTGTCACTGCGAGTCCTGATATAAATACTGTTGAACTTTGTGATGATGATGAGTTTATGGTCTTAGCATGTGATGGTATCTGGGATTGCATGTCAAGCCAGCAACTGGTAGATTTTGTGCATGAACAGCTAAGCTCGGAAAGCAAACTGTCTGTGGTGGTTGAAAGAGTCCTTGACAAATGTTTGGCACCATCAACTGCTGGTGGGGAGGGATGCGATAACATGACCATGATCTTGGTGCAATTCAAGAAGCCCATTGTGCCAGTTGAATCTGCGGGTAAGCAGCAGGAATCCTCAACAACCGAAGAAGAAGGACCTGGAACGGATGCAAATCCGAGTGAAAGTGAATCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

599

Amino Acids

65.35

Weight (kDa)

5.36

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 257 - 338 2e-10 Protein phosphatase 2C
PP2C PF00481 387 - 547 6.6e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 1630
AccB7I CCANNNNNTGG 2 cut(s) 1178, 1549
AccI GTMKAC 1 cut(s) 38
AciI CCGC 4 cut(s) 314, 322, 659, 1718
AclWI GGATC 2 cut(s) 57, 486
AcoI YGGCCR 1 cut(s) 874
AcsI RAATTY 5 cut(s) 440, 899, 1061, 1406, 1421
AcuI CTGAAG 1 cut(s) 765
AfiI CCNNNNNNNGG 2 cut(s) 1178, 1549
AjnI CCWGG 2 cut(s) 362, 1759
AloI GAACNNNNNNTCC 2 cut(s) 957, 989
Alw21I GWGCWC 4 cut(s) 90, 517, 651, 1187
Alw26I GTCTC 4 cut(s) 149, 634, 951, 1098
AlwI GGATC 2 cut(s) 57, 486
AlwNI CAGNNNCTG 2 cut(s) 238, 1549
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 3 cut(s) 660, 874, 1148
ApeKI GCWGC 4 cut(s) 185, 497, 824, 1726
ApoI RAATTY 5 cut(s) 440, 899, 1061, 1406, 1421
ArsI GACNNNNNNTTYG 4 cut(s) 127, 159, 248, 280
Asp700I GAANNNNTTC 1 cut(s) 1421
AspS9I GGNCC 3 cut(s) 1148, 1169, 1757
AsuC2I CCSGG 2 cut(s) 103, 571
AsuHPI GGTGA 3 cut(s) 911, 1250, 1409
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 1169, 1757
BalI TGGCCA 1 cut(s) 876
BanI GGYRCC 1 cut(s) 1630
BanII GRGCYC 1 cut(s) 651
Bbv12I GWGCWC 4 cut(s) 90, 517, 651, 1187
BbvI GCAGC 4 cut(s) 197, 484, 836, 1738
BccI CCATC 8 cut(s) 267, 397, 454, 743, 789, 866, 1511, 1642
BcgI CGANNNNNNTGC 8 cut(s) 95, 99, 129, 133, 824, 834, 858, 868
BciT130I CCWGG 2 cut(s) 364, 1761
BclI TGATCA 1 cut(s) 1117
BcnI CCSGG 2 cut(s) 103, 571
BcoDI GTCTC 4 cut(s) 149, 634, 951, 1098
BfaI CTAG 3 cut(s) 393, 839, 1260
BfmI CTRYAG 1 cut(s) 1392
BfuAI ACCTGC 1 cut(s) 179
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 5 cut(s) 186, 498, 660, 825, 1727
BlpI GCTNAGC 1 cut(s) 1574
BlsI GCNGC 5 cut(s) 187, 499, 661, 826, 1728
Bme1390I CCNGG 4 cut(s) 103, 364, 571, 1761
Bme18I GGWCC 2 cut(s) 1169, 1757
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 1148, 1169, 1757
BmiI GGNNCC 2 cut(s) 1149, 1632
BmrFI CCNGG 4 cut(s) 103, 364, 571, 1761
BmrI ACTGGG 1 cut(s) 255
BmsI GCATC 7 cut(s) 307, 805, 832, 854, 996, 1646, 1759
BmuI ACTGGG 1 cut(s) 255
BplI GAGNNNNNCTC 2 cut(s) 638, 670
BpmI CTGGAG 1 cut(s) 975
Bpu1102I GCTNAGC 1 cut(s) 1574
BpuEI CTTGAG 5 cut(s) 66, 665, 917, 1328, 1334
BpuMI CCSGG 2 cut(s) 103, 571
BsaBI GATNNNNATC 2 cut(s) 639, 1773
BsaI GGTCTC 2 cut(s) 149, 1098
BsaJI CCNNGG 2 cut(s) 877, 1097
BsaWI WCCGGW 2 cut(s) 169, 475
BsaXI ACNNNNNCTCC 2 cut(s) 873, 903
Bsc4I CCNNNNNNNGG 2 cut(s) 1178, 1549
Bse1I ACTGG 6 cut(s) 250, 541, 1072, 1430, 1554, 1706
Bse8I GATNNNNATC 2 cut(s) 639, 1773
BseBI CCWGG 2 cut(s) 364, 1761
BseDI CCNNGG 2 cut(s) 877, 1097
BseGI GGATG 7 cut(s) 322, 800, 820, 845, 1003, 1661, 1774
BseJI GATNNNNATC 2 cut(s) 639, 1773
BseLI CCNNNNNNNGG 2 cut(s) 1178, 1549
BseMII CTCAG 2 cut(s) 476, 730
BseNI ACTGG 6 cut(s) 250, 541, 1072, 1430, 1554, 1706
BseRI GAGGAG 2 cut(s) 1114, 1157
BseXI GCAGC 4 cut(s) 197, 484, 836, 1738
BseYI CCCAGC 1 cut(s) 762
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 662, 876, 1150
BshNI GGYRCC 1 cut(s) 1630
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 4 cut(s) 90, 517, 651, 1187
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 5 cut(s) 47, 102, 170, 476, 570
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 2 cut(s) 1178, 1549
BsmAI GTCTC 4 cut(s) 149, 634, 951, 1098
BsmFI GGGAC 1 cut(s) 570
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 3 cut(s) 662, 876, 1150
Bso31I GGTCTC 2 cut(s) 149, 1098
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 4 cut(s) 90, 517, 651, 1187
Bsp143I GATC 7 cut(s) 49, 478, 640, 835, 1117, 1291, 1674
Bsp1720I GCTNAGC 1 cut(s) 1574
Bsp19I CCATGG 1 cut(s) 877
BspACI CCGC 4 cut(s) 314, 322, 659, 1718
BspANI GGCC 3 cut(s) 662, 876, 1150
BspCNI CTCAG 2 cut(s) 477, 729
BspLI GGNNCC 2 cut(s) 1149, 1632
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 2 cut(s) 57, 486
BspQI GCTCTTC 1 cut(s) 656
BspT107I GGYRCC 1 cut(s) 1630
BspTNI GGTCTC 2 cut(s) 149, 1098
BsrI ACTGG 6 cut(s) 250, 541, 1072, 1430, 1554, 1706
BssECI CCNNGG 2 cut(s) 877, 1097
BssMI GATC 7 cut(s) 49, 478, 640, 835, 1117, 1291, 1674
BssT1I CCWWGG 1 cut(s) 877
Bst2UI CCWGG 2 cut(s) 364, 1761
Bst4CI ACNGT 2 cut(s) 1474, 1593
Bst6I CTCTTC 1 cut(s) 656
BstAPI GCANNNNNTGC 1 cut(s) 194
BstC8I GCNNGC 5 cut(s) 108, 322, 576, 801, 1544
BstDEI CTNAG 6 cut(s) 11, 429, 485, 716, 1507, 1574
BstDSI CCRYGG 1 cut(s) 877
BstF5I GGATG 7 cut(s) 322, 800, 820, 845, 1003, 1661, 1774
BstKTI GATC 7 cut(s) 52, 481, 643, 838, 1120, 1294, 1677
BstMAI GTCTC 4 cut(s) 149, 634, 951, 1098
BstMBI GATC 7 cut(s) 49, 478, 640, 835, 1117, 1291, 1674
BstMCI CGRYCG 1 cut(s) 119
BstNI CCWGG 2 cut(s) 364, 1761
BstNSI RCATGY 2 cut(s) 1515, 1537
BstSCI CCNGG 4 cut(s) 101, 362, 569, 1759
BstSFI CTRYAG 1 cut(s) 1392
BstV1I GCAGC 4 cut(s) 197, 484, 836, 1738
BstX2I RGATCY 1 cut(s) 49
BstXI CCANNNNNNTGG 1 cut(s) 1678
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 3 cut(s) 662, 876, 1150
BtgI CCRYGG 1 cut(s) 877
BtsCI GGATG 7 cut(s) 322, 800, 820, 845, 1003, 1661, 1774
BtsI GCAGTG 3 cut(s) 420, 1111, 1449
BtsIMutI CAGTG 5 cut(s) 420, 1111, 1171, 1437, 1449
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 5 cut(s) 108, 322, 576, 801, 1544
CaiI CAGNNNCTG 2 cut(s) 238, 1549
Cfr13I GGNCC 3 cut(s) 1148, 1169, 1757
DdeI CTNAG 6 cut(s) 11, 429, 485, 716, 1507, 1574
DpnI GATC 7 cut(s) 51, 480, 642, 837, 1119, 1293, 1676
DpnII GATC 7 cut(s) 49, 478, 640, 835, 1117, 1291, 1674
DraI TTTAAA 1 cut(s) 1332
EaeI YGGCCR 1 cut(s) 874
Eam1104I CTCTTC 1 cut(s) 656
EarI CTCTTC 1 cut(s) 656
Ecl136II GAGCTC 1 cut(s) 649
Eco130I CCWWGG 1 cut(s) 877
Eco24I GRGCYC 1 cut(s) 651
Eco31I GGTCTC 2 cut(s) 149, 1098
Eco32I GATATC 2 cut(s) 613, 1257
Eco47I GGWCC 2 cut(s) 1169, 1757
Eco53kI GAGCTC 1 cut(s) 649
Eco57I CTGAAG 1 cut(s) 765
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 649
EcoO109I RGGNCCY 2 cut(s) 1148, 1757
EcoRI GAATTC 2 cut(s) 440, 1406
EcoRII CCWGG 2 cut(s) 362, 1759
EcoRV GATATC 2 cut(s) 613, 1257
EcoT14I CCWWGG 1 cut(s) 877
EcoT22I ATGCAT 1 cut(s) 847
EcoT38I GRGCYC 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 877
FaqI GGGAC 1 cut(s) 570
FauI CCCGC 2 cut(s) 329, 1711
FauNDI CATATG 1 cut(s) 556
FbaI TGATCA 1 cut(s) 1117
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 5 cut(s) 186, 498, 660, 825, 1727
FokI GGATG 7 cut(s) 329, 807, 827, 832, 1010, 1668, 1781
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 5 cut(s) 186, 498, 660, 825, 1727
FspBI CTAG 3 cut(s) 393, 839, 1260
GluI GCNGC 5 cut(s) 186, 498, 660, 825, 1727
GsaI CCCAGC 1 cut(s) 766
GsuI CTGGAG 1 cut(s) 975
HaeIII GGCC 3 cut(s) 662, 876, 1150
HapII CCGG 5 cut(s) 47, 102, 170, 476, 570
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HindIII AAGCTT 1 cut(s) 981
HpaII CCGG 5 cut(s) 47, 102, 170, 476, 570
HphI GGTGA 3 cut(s) 911, 1250, 1409
Hpy166II GTNNAC 3 cut(s) 39, 424, 1013
Hpy188III TCNNGA 7 cut(s) 644, 833, 839, 934, 1287, 1460, 1690
Hpy8I GTNNAC 3 cut(s) 39, 424, 1013
HpyAV CCTTC 4 cut(s) 1073, 1258, 1312, 1748
HpyCH4III ACNGT 2 cut(s) 1474, 1593
HpyF3I CTNAG 6 cut(s) 11, 429, 485, 716, 1507, 1574
Ksp22I TGATCA 1 cut(s) 1117
Kzo9I GATC 7 cut(s) 49, 478, 640, 835, 1117, 1291, 1674
LguI GCTCTTC 1 cut(s) 656
LmnI GCTCC 2 cut(s) 1101, 1182
Lsp1109I GCAGC 4 cut(s) 197, 484, 836, 1738
LweI GCATC 7 cut(s) 307, 805, 832, 854, 996, 1646, 1759
MaeI CTAG 3 cut(s) 393, 839, 1260
MaeIII GTNAC 4 cut(s) 254, 490, 1397, 1447
MalI GATC 7 cut(s) 51, 480, 642, 837, 1119, 1293, 1676
MboI GATC 7 cut(s) 49, 478, 640, 835, 1117, 1291, 1674
MboII GAAGA 8 cut(s) 44, 288, 469, 643, 758, 908, 1760, 1763
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 4 cut(s) 90, 517, 651, 1187
MlsI TGGCCA 1 cut(s) 876
MluNI TGGCCA 1 cut(s) 876
MlyI GAGTC 5 cut(s) 73, 676, 1371, 1465, 1620
MmeI TCCRAC 2 cut(s) 1004, 1196
Mox20I TGGCCA 1 cut(s) 876
Mph1103I ATGCAT 1 cut(s) 847
MroXI GAANNNNTTC 1 cut(s) 1421
MscI TGGCCA 1 cut(s) 876
MseI TTAA 2 cut(s) 1085, 1331
MslI CAYNNNNRTG 3 cut(s) 486, 555, 1670
Msp20I TGGCCA 1 cut(s) 876
MspA1I CMGCKG 1 cut(s) 953
MspI CCGG 5 cut(s) 47, 102, 170, 476, 570
MspR9I CCNGG 4 cut(s) 103, 364, 571, 1761
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 2 cut(s) 364, 1761
NciI CCSGG 2 cut(s) 103, 571
NcoI CCATGG 1 cut(s) 877
NdeI CATATG 1 cut(s) 556
NdeII GATC 7 cut(s) 49, 478, 640, 835, 1117, 1291, 1674
NlaIV GGNNCC 2 cut(s) 1149, 1632
NmuCI GTSAC 4 cut(s) 254, 490, 1397, 1447
NsiI ATGCAT 1 cut(s) 847
NspI RCATGY 2 cut(s) 1515, 1537
PaeR7I CTCGAG 1 cut(s) 89
PciSI GCTCTTC 1 cut(s) 656
PctI GAATGC 1 cut(s) 415
PdmI GAANNNNTTC 1 cut(s) 1421
PfeI GAWTC 6 cut(s) 306, 1241, 1378, 1712, 1733, 1790
PflFI GACNNNGTC 1 cut(s) 141
PflMI CCANNNNNTGG 2 cut(s) 1178, 1549
PkrI GCNGC 5 cut(s) 187, 499, 661, 826, 1728
PleI GAGTC 5 cut(s) 73, 676, 1370, 1464, 1619
PpsI GAGTC 5 cut(s) 73, 676, 1370, 1464, 1619
PpuMI RGGWCCY 1 cut(s) 1757
PsiI TTATAA 1 cut(s) 74
Psp124BI GAGCTC 1 cut(s) 651
Psp5II RGGWCCY 1 cut(s) 1757
Psp6I CCWGG 2 cut(s) 362, 1759
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 2 cut(s) 362, 1759
PspN4I GGNNCC 2 cut(s) 1149, 1632
PspPI GGNCC 3 cut(s) 1148, 1169, 1757
PspPPI RGGWCCY 1 cut(s) 1757
PspXI VCTCGAGB 1 cut(s) 89
PstNI CAGNNNCTG 2 cut(s) 238, 1549
PsuI RGATCY 1 cut(s) 49
PsyI GACNNNGTC 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 953
RseI CAYNNNNRTG 3 cut(s) 486, 555, 1670
SacI GAGCTC 1 cut(s) 651
SalI GTCGAC 1 cut(s) 37
SapI GCTCTTC 1 cut(s) 656
SaqAI TTAA 2 cut(s) 1085, 1331
SatI GCNGC 5 cut(s) 186, 498, 660, 825, 1727
Sau3AI GATC 7 cut(s) 49, 478, 640, 835, 1117, 1291, 1674
Sau96I GGNCC 3 cut(s) 1148, 1169, 1757
SchI GAGTC 5 cut(s) 73, 676, 1371, 1465, 1620
ScrFI CCNGG 4 cut(s) 103, 364, 571, 1761
SduI GDGCHC 4 cut(s) 90, 517, 651, 1187
SfaNI GCATC 7 cut(s) 307, 805, 832, 854, 996, 1646, 1759
SfcI CTRYAG 1 cut(s) 1392
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 2 cut(s) 1169, 1757
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 3 cut(s) 486, 555, 1670
SmlI CTYRAG 6 cut(s) 81, 89, 644, 932, 1307, 1313
SmoI CTYRAG 6 cut(s) 81, 89, 644, 932, 1307, 1313
SsiI CCGC 4 cut(s) 314, 322, 659, 1718
SspI AATATT 1 cut(s) 70
SspMI CTAG 3 cut(s) 393, 839, 1260
SstI GAGCTC 1 cut(s) 651
StyD4I CCNGG 4 cut(s) 101, 362, 569, 1759
StyI CCWWGG 1 cut(s) 877
TaaI ACNGT 2 cut(s) 1474, 1593
TaqI TCGA 6 cut(s) 38, 90, 438, 471, 639, 680
TaqII GACCGA 1 cut(s) 105
TauI GCSGC 1 cut(s) 662
TfiI GAWTC 6 cut(s) 306, 1241, 1378, 1712, 1733, 1790
Tru1I TTAA 2 cut(s) 1085, 1331
Tru9I TTAA 2 cut(s) 1085, 1331
TscAI CASTG 5 cut(s) 420, 1111, 1171, 1437, 1456
TseFI GTSAC 4 cut(s) 254, 490, 1397, 1447
TseI GCWGC 4 cut(s) 185, 497, 824, 1726
Tsp45I GTSAC 4 cut(s) 254, 490, 1397, 1447
TspDTI ATGAA 4 cut(s) 470, 954, 1014, 1581
TspGWI ACGGA 2 cut(s) 243, 1781
TspRI CASTG 5 cut(s) 420, 1111, 1171, 1437, 1456
Tth111I GACNNNGTC 1 cut(s) 141
Van91I CCANNNNNTGG 2 cut(s) 1178, 1549
VpaK11BI GGWCC 2 cut(s) 1169, 1757
XapI RAATTY 5 cut(s) 440, 899, 1061, 1406, 1421
XbaI TCTAGA 1 cut(s) 838
XceI RCATGY 2 cut(s) 1515, 1537
XhoI CTCGAG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 38
XmnI GAANNNNTTC 1 cut(s) 1421
XspI CTAG 3 cut(s) 393, 839, 1260
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.