pycom08g04560

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000743
Physical Location & Seq
Forward (+)
3451262 .. 3452332
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04560.1

Sequence Viewer

Length: 888 bp
ATGGCTTCTGTGACCTGCAACTTCACTTCCCTCTCGCCATCATCCGGTGGTTCGGACCAGCGAAGTTGGAACCAAATTGCAAGGAATCAGGTGGTGAAGGGGAACGTGGAGCATGCATTTCAATCATACATTGAGATGCAGAAACTTGGTTTCTCTGCTGATAACTACACATTTCCCCTTCTGCTCAATGCAGCAGGTAATACTTCTTTGCGTATCGGGTTAACGCTCCATGGCCGGACAGTTAAAACCGGTTTCTGCGACCATCTTTTCGTCCAAACTGCTTTGCTGAAAGACTCCTTGTTAATGCTCGTAAGGTTCGATAAAATGCCCGAGAGAGATGTGGGAGCCTGGAACTCCATGCTAGATGTGTATGCTTCTAGCGGCCAGATGGATAACGCAATGGAAATTTTCGATACAATGCCGTCACAAGACCTTGCATCCTTCAATATAATGATCGATCCGGGTTTGCAGGCGCACAAAGCGTATCCTCTGCCAGAAGTATTTTCGATCGAATTCCTGAGAAAGATGTTGTCTCATGGAACGATGATATTGGCGTGCATGAACGGTGGAGACACGGCTGAAGCGGGAATGCCAGAGAGAAATGTCGCATCGAGTCCCCATCTGATAACCGCGCTGATAGACATGTATGCTAAGTGTGGAAGCATACACAACTCCTTAGAGGTGTCCTACAAGTCGAAAAACAAGGACATCTACTGCTGGACTGCCCTAATTTCCGGGCTCGCGCTTCATGGTTTTGGGTACGTTGTTCTGAAGCTCTTCGACCAAATGAGAGAGAACTGCATTAAACCAGATGACATCACCTTCATTGGGGTTTTAAGTGCTTGCAGCCATGCAGGTTTGGTGAAGGAGGGATGTGAAATCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

296

Amino Acids

32.46

Weight (kDa)

5.82

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 113 - 153 4.9e-06 PPR repeat family
PPR PF01535 116 - 142 4e-06 PPR repeat
PPR_2 PF13041 235 - 283 3e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 23, 185, 845
AccII CGCG 2 cut(s) 632, 743
AciI CCGC 3 cut(s) 381, 584, 630
AclWI GGATC 1 cut(s) 452
AcoI YGGCCR 2 cut(s) 232, 382
AcsI RAATTY 2 cut(s) 405, 512
AcuI CTGAAG 2 cut(s) 600, 791
AfaI GTAC 1 cut(s) 761
AfiI CCNNNNNNNGG 2 cut(s) 44, 828
AflIII ACRYGT 1 cut(s) 642
AgeI ACCGGT 1 cut(s) 248
AgsI TTSAA 2 cut(s) 122, 445
AjnI CCWGG 1 cut(s) 347
AluBI AGCT 1 cut(s) 775
AluI AGCT 1 cut(s) 775
Alw26I GTCTC 2 cut(s) 537, 564
AlwI GGATC 1 cut(s) 452
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 2 cut(s) 232, 382
ApeKI GCWGC 2 cut(s) 191, 846
ApoI RAATTY 2 cut(s) 405, 512
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AsiGI ACCGGT 1 cut(s) 248
Asp700I GAANNNNTTC 1 cut(s) 776
AspLEI GCGC 3 cut(s) 475, 634, 745
AspS9I GGNCC 1 cut(s) 55
AsuC2I CCSGG 2 cut(s) 462, 736
AsuHPI GGTGA 3 cut(s) 106, 811, 874
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 1 cut(s) 55
BanII GRGCYC 1 cut(s) 741
BbvI GCAGC 2 cut(s) 203, 858
BccI CCATC 4 cut(s) 46, 270, 382, 627
BceAI ACGGC 2 cut(s) 406, 591
BciT130I CCWGG 1 cut(s) 349
BciVI GTATCC 1 cut(s) 495
BcnI CCSGG 2 cut(s) 462, 736
BcoDI GTCTC 2 cut(s) 537, 564
BfaI CTAG 2 cut(s) 362, 378
BfuAI ACCTGC 3 cut(s) 23, 185, 845
BfuI GTATCC 1 cut(s) 495
BisI GCNGC 3 cut(s) 192, 382, 847
BlsI GCNGC 3 cut(s) 193, 383, 848
Bme1390I CCNGG 3 cut(s) 349, 462, 736
Bme18I GGWCC 1 cut(s) 55
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 2 cut(s) 71, 346
BmrFI CCNGG 3 cut(s) 349, 462, 736
BmsI GCATC 3 cut(s) 126, 446, 617
BpuMI CCSGG 2 cut(s) 462, 736
Bsa29I ATCGAT 1 cut(s) 456
BsaJI CCNNGG 1 cut(s) 229
BsaWI WCCGGW 2 cut(s) 44, 248
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 828
Bse118I RCCGGY 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 1 cut(s) 349
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 3 cut(s) 41, 437, 878
BseLI CCNNNNNNNGG 2 cut(s) 44, 828
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 509
BseXI GCAGC 2 cut(s) 203, 858
Bsh1236I CGCG 2 cut(s) 632, 743
Bsh1285I CGRYCG 1 cut(s) 510
BshFI GGCC 2 cut(s) 234, 384
BshTI ACCGGT 1 cut(s) 248
BshVI ATCGAT 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 5 cut(s) 45, 235, 249, 461, 735
BslFI GGGAC 1 cut(s) 600
BslI CCNNNNNNNGG 2 cut(s) 44, 828
BsmAI GTCTC 2 cut(s) 537, 564
BsmFI GGGAC 1 cut(s) 600
BsmI GAATGC 1 cut(s) 594
BsnI GGCC 2 cut(s) 234, 384
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 741
Bsp143I GATC 3 cut(s) 453, 457, 507
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 3 cut(s) 381, 584, 630
BspANI GGCC 2 cut(s) 234, 384
BspCNI CTCAG 1 cut(s) 510
BspDI ATCGAT 1 cut(s) 456
BspFNI CGCG 2 cut(s) 632, 743
BspLI GGNNCC 2 cut(s) 71, 346
BspMI ACCTGC 3 cut(s) 23, 185, 845
BspPI GGATC 1 cut(s) 452
BspQI GCTCTTC 1 cut(s) 782
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 248
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 3 cut(s) 453, 457, 507
BssT1I CCWWGG 1 cut(s) 229
Bst2UI CCWGG 1 cut(s) 349
Bst4CI ACNGT 2 cut(s) 241, 566
Bst6I CTCTTC 1 cut(s) 782
BstC8I GCNNGC 5 cut(s) 114, 471, 556, 741, 844
BstDEI CTNAG 3 cut(s) 518, 651, 676
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 3 cut(s) 41, 437, 878
BstFNI CGCG 2 cut(s) 632, 743
BstHHI GCGC 3 cut(s) 475, 634, 745
BstKTI GATC 3 cut(s) 456, 460, 510
BstMAI GTCTC 2 cut(s) 537, 564
BstMBI GATC 3 cut(s) 453, 457, 507
BstMCI CGRYCG 1 cut(s) 510
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 349
BstNSI RCATGY 2 cut(s) 116, 646
BstSCI CCNGG 3 cut(s) 347, 460, 734
BstUI CGCG 2 cut(s) 632, 743
BstV1I GCAGC 2 cut(s) 203, 858
Bsu15I ATCGAT 1 cut(s) 456
BsuI GTATCC 1 cut(s) 495
BsuRI GGCC 2 cut(s) 234, 384
BsuTUI ATCGAT 1 cut(s) 456
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 3 cut(s) 41, 437, 878
BveI ACCTGC 3 cut(s) 23, 185, 845
Cac8I GCNNGC 5 cut(s) 114, 471, 556, 741, 844
CfoI GCGC 3 cut(s) 475, 634, 745
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 55
ClaI ATCGAT 1 cut(s) 456
Csp6I GTAC 1 cut(s) 760
CspAI ACCGGT 1 cut(s) 248
CviAII CATG 8 cut(s) 113, 230, 358, 536, 559, 643, 749, 851
CviJI RGCY 8 cut(s) 5, 234, 347, 384, 578, 739, 775, 849
CviKI_1 RGCY 8 cut(s) 5, 234, 347, 384, 578, 739, 775, 849
CviQI GTAC 1 cut(s) 760
DdeI CTNAG 3 cut(s) 518, 651, 676
DpnI GATC 3 cut(s) 455, 459, 509
DpnII GATC 3 cut(s) 453, 457, 507
EaeI YGGCCR 2 cut(s) 232, 382
Eam1104I CTCTTC 1 cut(s) 782
EarI CTCTTC 1 cut(s) 782
Eco130I CCWWGG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 741
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 600, 791
Eco88I CYCGRG 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 347
EcoT14I CCWWGG 1 cut(s) 229
EcoT22I ATGCAT 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 741
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 8 cut(s) 116, 233, 361, 539, 562, 646, 752, 854
FaqI GGGAC 1 cut(s) 600
FatI CATG 8 cut(s) 112, 229, 357, 535, 558, 642, 748, 850
FauI CCCGC 1 cut(s) 577
Fnu4HI GCNGC 3 cut(s) 192, 382, 847
FokI GGATG 2 cut(s) 28, 424
FriOI GRGCYC 1 cut(s) 741
Fsp4HI GCNGC 3 cut(s) 192, 382, 847
FspBI CTAG 2 cut(s) 362, 378
GlaI GCGC 3 cut(s) 474, 633, 744
GluI GCNGC 3 cut(s) 192, 382, 847
HaeIII GGCC 2 cut(s) 234, 384
HapII CCGG 5 cut(s) 45, 235, 249, 461, 735
HhaI GCGC 3 cut(s) 475, 634, 745
Hin1II CATG 8 cut(s) 116, 233, 361, 539, 562, 646, 752, 854
Hin6I GCGC 3 cut(s) 473, 632, 743
HinP1I GCGC 3 cut(s) 473, 632, 743
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 3 cut(s) 85, 293, 613
HpaI GTTAAC 1 cut(s) 222
HpaII CCGG 5 cut(s) 45, 235, 249, 461, 735
HphI GGTGA 3 cut(s) 106, 811, 874
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 3 cut(s) 55, 624, 771
Hpy188III TCNNGA 1 cut(s) 517
Hpy8I GTNNAC 1 cut(s) 222
HpyAV CCTTC 5 cut(s) 91, 188, 451, 832, 859
HpyCH4III ACNGT 2 cut(s) 241, 566
HpyCH4IV ACGT 2 cut(s) 105, 762
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 3 cut(s) 518, 651, 676
HpySE526I ACGT 2 cut(s) 105, 762
Hsp92II CATG 8 cut(s) 116, 233, 361, 539, 562, 646, 752, 854
HspAI GCGC 3 cut(s) 473, 632, 743
KspAI GTTAAC 1 cut(s) 222
Kzo9I GATC 3 cut(s) 453, 457, 507
LguI GCTCTTC 1 cut(s) 782
LmnI GCTCC 3 cut(s) 109, 231, 344
Lsp1109I GCAGC 2 cut(s) 203, 858
LweI GCATC 3 cut(s) 126, 446, 617
MaeI CTAG 2 cut(s) 362, 378
MaeII ACGT 2 cut(s) 105, 762
MaeIII GTNAC 2 cut(s) 10, 423
MalI GATC 3 cut(s) 455, 459, 509
MboI GATC 3 cut(s) 453, 457, 507
MboII GAAGA 1 cut(s) 769
MhlI GDGCHC 1 cut(s) 741
MluCI AATT 4 cut(s) 75, 405, 512, 729
MlyI GAGTC 2 cut(s) 287, 622
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 4 cut(s) 41, 498, 673, 862
Mph1103I ATGCAT 1 cut(s) 118
MroXI GAANNNNTTC 1 cut(s) 776
MseI TTAA 6 cut(s) 221, 243, 302, 804, 836, 886
MslI CAYNNNNRTG 1 cut(s) 134
MspI CCGG 5 cut(s) 45, 235, 249, 461, 735
MspR9I CCNGG 3 cut(s) 349, 462, 736
Mva1269I GAATGC 1 cut(s) 594
MvaI CCWGG 1 cut(s) 349
MvnI CGCG 2 cut(s) 632, 743
MwoI GCNNNNNNNGC 1 cut(s) 479
NciI CCSGG 2 cut(s) 462, 736
NcoI CCATGG 1 cut(s) 229
NdeII GATC 3 cut(s) 453, 457, 507
NlaIII CATG 8 cut(s) 116, 233, 361, 539, 562, 646, 752, 854
NlaIV GGNNCC 2 cut(s) 71, 346
NmuCI GTSAC 2 cut(s) 10, 423
NsiI ATGCAT 1 cut(s) 118
NspI RCATGY 2 cut(s) 116, 646
PaeI GCATGC 1 cut(s) 116
PciI ACATGT 1 cut(s) 642
PciSI GCTCTTC 1 cut(s) 782
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 594
PdmI GAANNNNTTC 1 cut(s) 776
PfeI GAWTC 1 cut(s) 85
PinAI ACCGGT 1 cut(s) 248
PkrI GCNGC 3 cut(s) 193, 383, 848
Ple19I CGATCG 1 cut(s) 510
PleI GAGTC 2 cut(s) 287, 621
PpsI GAGTC 2 cut(s) 287, 621
PscI ACATGT 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 347
PspGI CCWGG 1 cut(s) 347
PspN4I GGNNCC 2 cut(s) 71, 346
PspPI GGNCC 1 cut(s) 55
PvuI CGATCG 1 cut(s) 510
RsaI GTAC 1 cut(s) 761
RsaNI GTAC 1 cut(s) 760
RseI CAYNNNNRTG 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 782
SaqAI TTAA 6 cut(s) 221, 243, 302, 804, 836, 886
SatI GCNGC 3 cut(s) 192, 382, 847
Sau3AI GATC 3 cut(s) 453, 457, 507
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 2 cut(s) 287, 622
ScrFI CCNGG 3 cut(s) 349, 462, 736
SduI GDGCHC 1 cut(s) 741
SfaNI GCATC 3 cut(s) 126, 446, 617
SinI GGWCC 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 134
SphI GCATGC 1 cut(s) 116
Sse9I AATT 4 cut(s) 75, 405, 512, 729
SsiI CCGC 3 cut(s) 381, 584, 630
SspMI CTAG 2 cut(s) 362, 378
StyD4I CCNGG 3 cut(s) 347, 460, 734
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 2 cut(s) 241, 566
TaiI ACGT 2 cut(s) 108, 765
TaqI TCGA 8 cut(s) 318, 411, 456, 506, 510, 611, 695, 780
TasI AATT 4 cut(s) 75, 405, 512, 729
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 6 cut(s) 221, 243, 302, 804, 836, 886
Tru9I TTAA 6 cut(s) 221, 243, 302, 804, 836, 886
TseFI GTSAC 2 cut(s) 10, 423
TseI GCWGC 2 cut(s) 191, 846
Tsp45I GTSAC 2 cut(s) 10, 423
TspDTI ATGAA 3 cut(s) 575, 737, 814
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 2 cut(s) 405, 512
XceI RCATGY 2 cut(s) 116, 646
XmnI GAANNNNTTC 1 cut(s) 776
XspI CTAG 2 cut(s) 362, 378
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.