RchiOBHm_Chr6g0299331

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000743
Physical Location & Seq
Forward (+)
60458554 .. 60464016
5463 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26877

Sequence Viewer

Length: 1098 bp
ATGGGTATATATCTCAGCACGCCCAAAACTGACAAGTTCTCTGAAGATGGAGAGAATGGCTGGGTCAGATATGGTCTATCGTCCATGCAAGGATGGCGTGCAACTATGGAGGATGCTCATGCAGCATATCCCGATCTAGATGCATCCACCTCGTTCTTTGGTGTTTATGATGGCCATGGAGGAAAGGTAGTTGCTAAATTCTGTGCCAAATATCTTCACCGACAAATGCTCAAGAATGACGCATATTCAGCTGGAGACATAGGAACTTCTGTTCAAAAAGCTTTTTTCAGAATGGATGAAATGATGCGTGGACAAAGGGGTTGGAGAGAGTTAGCTGTTCTGGGAGATAAGATAAACAAATTTACTGGCATGATAGAAGGGTTAATCTGGTCTCCGAGGAGCAGTGAGGGTAATGATCAAGCTGATGATTGGGCTTTTGAGGAGGGGCCTCACTCTGATTTCAGTGGTCCAACTTCTGGGAGCACAGCTTGTGTTGCAATTATTAGAAACAACCAACTTGTTGTTGCAAATGCTGGTGATTCTCGTTGTGTGATATCTAGGAAGGGTCAGGCATATAACCTGTCCAGAGATCACAAACCTGAACTTGAGCTTGAGAAGGAAAGGATTTTAAAAGCTGGTGGTTTTATCCATGCAGGACGAGTCAATGGCAGTTTGAATCTTGCAAGAGCTATAGGTGACATGGAATTCAAGCAGAATAAATTCTTGCCAGTGGAAAAGCAAATTGTCACTGCGAGTCCTGATATAAATACTGTTGAACTTTGTGATGATGATGAGTTTATGGTCTTAGCATGTGATGGTATCTGGGATTGCATGTCAAGCCAGCAACTGGTAGATTTTGTGCATGAACAGCTAAGCTCGGAAAGCAAACTGTCTGTGGTGGTTGAAAGAGTCCTTGACAAATGTTTGGCACCATCGACTGCTGGTGGGGAGGGATGCGATAACATGACCATGATCTTGGTGCAATTCAAGAAGCCCATTGTGCCAGTTGAATCTGCGGGTAAGCAGCAGGAATCCTCAACAACCGAAGAAGAAGGACCTGGAACGGATGCAAATCCAAGTGAAAGTGAATCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

365

Amino Acids

40.01

Weight (kDa)

4.86

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 23 - 104 1.1e-10 Protein phosphatase 2C
PP2C PF00481 153 - 313 2.3e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 928
AccB7I CCANNNNNTGG 2 cut(s) 476, 847
AciI CCGC 1 cut(s) 1016
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 4 cut(s) 197, 359, 704, 719
AcuI CTGAAG 1 cut(s) 63
AfiI CCNNNNNNNGG 2 cut(s) 476, 847
AgsI TTSAA 7 cut(s) 275, 676, 709, 776, 905, 988, 1010
AjnI CCWGG 1 cut(s) 1057
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
Alw21I GWGCWC 1 cut(s) 485
Alw26I GTCTC 2 cut(s) 249, 396
AlwNI CAGNNNCTG 1 cut(s) 847
AoxI GGCC 2 cut(s) 172, 446
ApeKI GCWGC 2 cut(s) 122, 1024
ApoI RAATTY 4 cut(s) 197, 359, 704, 719
Asp700I GAANNNNTTC 1 cut(s) 719
AspS9I GGNCC 3 cut(s) 446, 467, 1055
AsuHPI GGTGA 3 cut(s) 209, 548, 707
AvaII GGWCC 2 cut(s) 467, 1055
BalI TGGCCA 1 cut(s) 174
BanI GGYRCC 1 cut(s) 928
Bbv12I GWGCWC 1 cut(s) 485
BbvI GCAGC 2 cut(s) 134, 1036
BccI CCATC 5 cut(s) 41, 87, 164, 809, 940
BcgI CGANNNNNNTGC 4 cut(s) 122, 132, 156, 166
BciT130I CCWGG 1 cut(s) 1059
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 2 cut(s) 249, 396
BfaI CTAG 2 cut(s) 137, 558
BfmI CTRYAG 1 cut(s) 690
BisI GCNGC 2 cut(s) 123, 1025
BlpI GCTNAGC 1 cut(s) 872
BlsI GCNGC 2 cut(s) 124, 1026
Bme1390I CCNGG 1 cut(s) 1059
Bme18I GGWCC 2 cut(s) 467, 1055
BmgT120I GGNCC 3 cut(s) 446, 467, 1055
BmiI GGNNCC 2 cut(s) 447, 930
BmrFI CCNGG 1 cut(s) 1059
BmsI GCATC 6 cut(s) 103, 130, 152, 294, 944, 1057
BpmI CTGGAG 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 872
BpuEI CTTGAG 3 cut(s) 215, 626, 632
BsaBI GATNNNNATC 1 cut(s) 1071
BsaI GGTCTC 1 cut(s) 396
BsaJI CCNNGG 2 cut(s) 175, 395
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 847
Bse1I ACTGG 4 cut(s) 370, 728, 852, 1004
Bse8I GATNNNNATC 1 cut(s) 1071
BseBI CCWGG 1 cut(s) 1059
BseDI CCNNGG 2 cut(s) 175, 395
BseGI GGATG 6 cut(s) 98, 118, 143, 301, 959, 1072
BseJI GATNNNNATC 1 cut(s) 1071
BseLI CCNNNNNNNGG 2 cut(s) 476, 847
BseMII CTCAG 1 cut(s) 28
BseNI ACTGG 4 cut(s) 370, 728, 852, 1004
BseRI GAGGAG 2 cut(s) 412, 455
BseXI GCAGC 2 cut(s) 134, 1036
BseYI CCCAGC 1 cut(s) 60
BshFI GGCC 2 cut(s) 174, 448
BshNI GGYRCC 1 cut(s) 928
BsiHKAI GWGCWC 1 cut(s) 485
BslI CCNNNNNNNGG 2 cut(s) 476, 847
BsmAI GTCTC 2 cut(s) 249, 396
BsnI GGCC 2 cut(s) 174, 448
Bso31I GGTCTC 1 cut(s) 396
Bsp1286I GDGCHC 1 cut(s) 485
Bsp143I GATC 4 cut(s) 133, 415, 589, 972
Bsp1720I GCTNAGC 1 cut(s) 872
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 1016
BspANI GGCC 2 cut(s) 174, 448
BspCNI CTCAG 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 447, 930
BspT107I GGYRCC 1 cut(s) 928
BspTNI GGTCTC 1 cut(s) 396
BsrI ACTGG 4 cut(s) 370, 728, 852, 1004
BssECI CCNNGG 2 cut(s) 175, 395
BssMI GATC 4 cut(s) 133, 415, 589, 972
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 1 cut(s) 1059
Bst4CI ACNGT 2 cut(s) 772, 891
BstC8I GCNNGC 3 cut(s) 20, 99, 842
BstDEI CTNAG 3 cut(s) 14, 805, 872
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 6 cut(s) 98, 118, 143, 301, 959, 1072
BstKTI GATC 4 cut(s) 136, 418, 592, 975
BstMAI GTCTC 2 cut(s) 249, 396
BstMBI GATC 4 cut(s) 133, 415, 589, 972
BstMWI GCNNNNNNNGC 8 cut(s) 94, 122, 248, 494, 837, 868, 882, 1000
BstNI CCWGG 1 cut(s) 1059
BstNSI RCATGY 2 cut(s) 813, 835
BstSCI CCNGG 1 cut(s) 1057
BstSFI CTRYAG 1 cut(s) 690
BstV1I GCAGC 2 cut(s) 134, 1036
BstXI CCANNNNNNTGG 1 cut(s) 976
BsuRI GGCC 2 cut(s) 174, 448
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 6 cut(s) 98, 118, 143, 301, 959, 1072
BtsI GCAGTG 2 cut(s) 409, 747
BtsIMutI CAGTG 4 cut(s) 409, 469, 735, 747
Cac8I GCNNGC 3 cut(s) 20, 99, 842
CaiI CAGNNNCTG 1 cut(s) 847
Cfr13I GGNCC 3 cut(s) 446, 467, 1055
CseI GACGC 1 cut(s) 248
DdeI CTNAG 3 cut(s) 14, 805, 872
DpnI GATC 4 cut(s) 135, 417, 591, 974
DpnII GATC 4 cut(s) 133, 415, 589, 972
DraI TTTAAA 1 cut(s) 630
EaeI YGGCCR 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 396
Eco32I GATATC 1 cut(s) 555
Eco47I GGWCC 2 cut(s) 467, 1055
Eco57I CTGAAG 1 cut(s) 63
EcoO109I RGGNCCY 2 cut(s) 446, 1055
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 1 cut(s) 1057
EcoRV GATATC 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 175
EcoT22I ATGCAT 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 175
FauI CCCGC 1 cut(s) 1009
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 2 cut(s) 123, 1025
FokI GGATG 6 cut(s) 105, 125, 130, 308, 966, 1079
Fsp4HI GCNGC 2 cut(s) 123, 1025
FspBI CTAG 2 cut(s) 137, 558
GluI GCNGC 2 cut(s) 123, 1025
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 2 cut(s) 174, 448
HgaI GACGC 1 cut(s) 248
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 8 cut(s) 539, 660, 676, 754, 909, 1010, 1031, 1088
HphI GGTGA 3 cut(s) 209, 548, 707
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 6 cut(s) 43, 68, 290, 396, 457, 880
Hpy188III TCNNGA 6 cut(s) 131, 137, 232, 585, 758, 988
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 4 cut(s) 371, 556, 610, 1046
HpyCH4III ACNGT 2 cut(s) 772, 891
HpyF10VI GCNNNNNNNGC 8 cut(s) 94, 122, 248, 494, 837, 868, 882, 1000
HpyF3I CTNAG 3 cut(s) 14, 805, 872
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 4 cut(s) 133, 415, 589, 972
LmnI GCTCC 2 cut(s) 399, 480
Lsp1109I GCAGC 2 cut(s) 134, 1036
LweI GCATC 6 cut(s) 103, 130, 152, 294, 944, 1057
MaeI CTAG 2 cut(s) 137, 558
MaeIII GTNAC 2 cut(s) 695, 745
MalI GATC 4 cut(s) 135, 417, 591, 974
MboI GATC 4 cut(s) 133, 415, 589, 972
MboII GAAGA 4 cut(s) 56, 206, 1058, 1061
MhlI GDGCHC 1 cut(s) 485
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 8 cut(s) 197, 359, 498, 704, 719, 741, 983, 1093
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 3 cut(s) 669, 763, 918
MmeI TCCRAC 2 cut(s) 302, 494
Mox20I TGGCCA 1 cut(s) 174
Mph1103I ATGCAT 1 cut(s) 145
MroXI GAANNNNTTC 1 cut(s) 719
MscI TGGCCA 1 cut(s) 174
MseI TTAA 2 cut(s) 383, 629
MslI CAYNNNNRTG 1 cut(s) 968
Msp20I TGGCCA 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 1059
MvaI CCWGG 1 cut(s) 1059
MwoI GCNNNNNNNGC 8 cut(s) 94, 122, 248, 494, 837, 868, 882, 1000
NcoI CCATGG 1 cut(s) 175
NdeII GATC 4 cut(s) 133, 415, 589, 972
NlaIV GGNNCC 2 cut(s) 447, 930
NmuCI GTSAC 2 cut(s) 695, 745
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 2 cut(s) 813, 835
PdmI GAANNNNTTC 1 cut(s) 719
PfeI GAWTC 5 cut(s) 539, 676, 1010, 1031, 1088
PflMI CCANNNNNTGG 2 cut(s) 476, 847
PkrI GCNGC 2 cut(s) 124, 1026
PleI GAGTC 3 cut(s) 668, 762, 917
PpsI GAGTC 3 cut(s) 668, 762, 917
PpuMI RGGWCCY 1 cut(s) 1055
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 1 cut(s) 1057
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 1 cut(s) 1057
PspN4I GGNNCC 2 cut(s) 447, 930
PspPI GGNCC 3 cut(s) 446, 467, 1055
PspPPI RGGWCCY 1 cut(s) 1055
PstNI CAGNNNCTG 1 cut(s) 847
PvuII CAGCTG 1 cut(s) 251
RseI CAYNNNNRTG 1 cut(s) 968
SaqAI TTAA 2 cut(s) 383, 629
SatI GCNGC 2 cut(s) 123, 1025
Sau3AI GATC 4 cut(s) 133, 415, 589, 972
Sau96I GGNCC 3 cut(s) 446, 467, 1055
SchI GAGTC 3 cut(s) 669, 763, 918
ScrFI CCNGG 1 cut(s) 1059
SduI GDGCHC 1 cut(s) 485
SfaNI GCATC 6 cut(s) 103, 130, 152, 294, 944, 1057
SfcI CTRYAG 1 cut(s) 690
SinI GGWCC 2 cut(s) 467, 1055
SmiMI CAYNNNNRTG 1 cut(s) 968
SmlI CTYRAG 3 cut(s) 230, 605, 611
SmoI CTYRAG 3 cut(s) 230, 605, 611
Sse9I AATT 8 cut(s) 197, 359, 498, 704, 719, 741, 983, 1093
SsiI CCGC 1 cut(s) 1016
SspMI CTAG 2 cut(s) 137, 558
StyD4I CCNGG 1 cut(s) 1057
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 2 cut(s) 772, 891
TaqI TCGA 1 cut(s) 935
TasI AATT 8 cut(s) 197, 359, 498, 704, 719, 741, 983, 1093
TfiI GAWTC 5 cut(s) 539, 676, 1010, 1031, 1088
Tru1I TTAA 2 cut(s) 383, 629
Tru9I TTAA 2 cut(s) 383, 629
TscAI CASTG 4 cut(s) 409, 469, 735, 754
TseFI GTSAC 2 cut(s) 695, 745
TseI GCWGC 2 cut(s) 122, 1024
Tsp45I GTSAC 2 cut(s) 695, 745
TspDTI ATGAA 2 cut(s) 312, 879
TspGWI ACGGA 1 cut(s) 1079
TspRI CASTG 4 cut(s) 409, 469, 735, 754
Van91I CCANNNNNTGG 2 cut(s) 476, 847
VpaK11BI GGWCC 2 cut(s) 467, 1055
XapI RAATTY 4 cut(s) 197, 359, 704, 719
XbaI TCTAGA 1 cut(s) 136
XceI RCATGY 2 cut(s) 813, 835
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 2 cut(s) 137, 558
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.