MD17G1224700.v1.1

protein phosphatase 2C 60

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000743
Physical Location & Seq
Reverse (-)
27310584 .. 27325324
14741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1224700.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGCAAGGGTGGCGTTCAACCATGGAAGATGCTCATGCTGCTTATCCAGATTTTGACGGTTCAACATCTTTCTTTGGTGTTTATGATGGCCATGGAGGTAAAGCAGTGGCAAATTTCTGTGCCAAATATCTCCACCAACAGGTGCTCAAGGATGAAGCATATCTAGCTGGTGATTTAGGCAGTTCTCTGCAGAAAGCTTTCCTCAGGATGGATGAGATGATGCGTGGACAAAGAGGCTGGAGAGAATTAGCCATACTGGGAGATAAGATAGACAAAGTTTCTGGTCTTATAGAAGGGTTCATTTTTTCTCCAAAGAGTGTTGAAGCTAAGAGTAGTGCATTCAATGACCATGTTGATCAGTGGTCTTCTGAGGAGGGGCCTCATTCTGATTTTGATGGACCAAATTCCGGAAGTACAGCTTGTGTTGCTATTATCCGAAACAAACAACTTCTTGTTGCCAATGCTGGTGATTCTCGCTGCGTAATATCCAGGAAGGGTGAGGCATATAATTTGTCTAAAGATCATAAGCCTGACATTGAGAATGAGAAAGAAAGAATTCTGAAGGCTGGTGGATTTATCCAAGTTGGAAGGGTCAATGGAAGTTTGAACTTGGCAAGAGCAATTGGGGATGCGGAATTCAAGCAGAACAAACAGTTGCCCGTTGAAAAGCAGATTGTGACTGCCAATCCTGACATAAATAGTGTTGAGCTTTGCGATGATGATGAGTTTCTGGTCATAGCTTGTGATGGGATATGGGACTGCATGTCAAGTCAACAACTTGTGGACTATGTCCGGGAACAATTAAAACATGAAAGTAAACTGTCAGTGGTTTGCGAGAGAGTGTTTGAAAGGTGCTTGGCGCCATCATCTGGTGGAGAGGGATGTGACAACATGACAATGATCTTGGTTCAGTTTAAGAAACCTGTTGCTTCAGCTGCATCTGTTGGGAACCAGCCCATCGCGTCTAATCCGCCATCCGAGAAGGAAAAAACCGCAGCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

335

Amino Acids

36.52

Weight (kDa)

5.21

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 1 - 76 4.9e-11 Protein phosphatase 2C
PP2C PF00481 114 - 290 1.9e-59 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 859
AccB7I CCANNNNNTGG 1 cut(s) 869
AccII CGCG 1 cut(s) 962
AccIII TCCGGA 1 cut(s) 407
AciI CCGC 3 cut(s) 632, 971, 993
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 4 cut(s) 112, 403, 555, 635
AcuI CTGAAG 2 cut(s) 581, 915
AcyI GRCGYC 1 cut(s) 860
AfaI GTAC 1 cut(s) 415
AfiI CCNNNNNNNGG 4 cut(s) 139, 208, 407, 869
AgsI TTSAA 8 cut(s) 18, 63, 323, 343, 607, 640, 665, 848
AhdI GACNNNNNGTC 1 cut(s) 763
AjnI CCWGG 1 cut(s) 488
AluBI AGCT 7 cut(s) 167, 197, 326, 419, 709, 740, 935
AluI AGCT 7 cut(s) 167, 197, 326, 419, 709, 740, 935
Alw21I GWGCWC 1 cut(s) 147
Aor13HI TCCGGA 1 cut(s) 407
AoxI GGCC 2 cut(s) 88, 377
ApeKI GCWGC 4 cut(s) 38, 477, 935, 995
ApoI RAATTY 4 cut(s) 112, 403, 555, 635
Asp700I GAANNNNTTC 2 cut(s) 197, 555
AspLEI GCGC 1 cut(s) 862
AspS9I GGNCC 2 cut(s) 377, 398
AsuC2I CCSGG 1 cut(s) 794
AsuHPI GGTGA 3 cut(s) 182, 479, 509
AvaII GGWCC 1 cut(s) 398
AxyI CCTNAGG 1 cut(s) 203
BalI TGGCCA 1 cut(s) 90
BanI GGYRCC 1 cut(s) 859
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 3 cut(s) 25, 464, 922
BccI CCATC 7 cut(s) 80, 202, 389, 740, 871, 965, 982
BciT130I CCWGG 1 cut(s) 490
BclI TGATCA 1 cut(s) 355
BcnI CCSGG 1 cut(s) 794
BfaI CTAG 1 cut(s) 164
BfmI CTRYAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 863
BisI GCNGC 4 cut(s) 39, 478, 936, 996
BlsI GCNGC 4 cut(s) 40, 479, 937, 997
Bme1390I CCNGG 2 cut(s) 490, 794
Bme18I GGWCC 1 cut(s) 398
BmeRI GACNNNNNGTC 1 cut(s) 763
BmgT120I GGNCC 2 cut(s) 377, 398
BmiI GGNNCC 3 cut(s) 378, 861, 950
BmrFI CCNGG 2 cut(s) 490, 794
BmrI ACTGGG 1 cut(s) 266
BmsI GCATC 4 cut(s) 19, 210, 619, 947
BmuI ACTGGG 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 794
BsaHI GRCGYC 1 cut(s) 860
BsaJI CCNNGG 2 cut(s) 21, 91
BsaWI WCCGGW 1 cut(s) 407
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 208, 407, 869
Bse1I ACTGG 1 cut(s) 261
Bse21I CCTNAGG 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 407
BseBI CCWGG 1 cut(s) 490
BseDI CCNNGG 2 cut(s) 21, 91
BseGI GGATG 6 cut(s) 157, 213, 217, 634, 887, 974
BseLI CCNNNNNNNGG 4 cut(s) 139, 208, 407, 869
BseMII CTCAG 2 cut(s) 217, 360
BseNI ACTGG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 386
BseXI GCAGC 3 cut(s) 25, 464, 922
Bsh1236I CGCG 1 cut(s) 962
BshFI GGCC 2 cut(s) 90, 379
BshNI GGYRCC 1 cut(s) 859
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 2 cut(s) 408, 793
BslFI GGGAC 1 cut(s) 770
BslI CCNNNNNNNGG 4 cut(s) 139, 208, 407, 869
BsmFI GGGAC 1 cut(s) 770
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 2 cut(s) 90, 379
Bsp1286I GDGCHC 1 cut(s) 147
Bsp13I TCCGGA 1 cut(s) 407
Bsp143I GATC 3 cut(s) 355, 520, 900
Bsp19I CCATGG 2 cut(s) 21, 91
BspACI CCGC 3 cut(s) 632, 971, 993
BspANI GGCC 2 cut(s) 90, 379
BspCNI CTCAG 2 cut(s) 216, 361
BspEI TCCGGA 1 cut(s) 407
BspFNI CGCG 1 cut(s) 962
BspLI GGNNCC 3 cut(s) 378, 861, 950
BspMAI CTGCAG 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 859
BsrI ACTGG 1 cut(s) 261
BssECI CCNNGG 2 cut(s) 21, 91
BssMI GATC 3 cut(s) 355, 520, 900
BssNI GRCGYC 1 cut(s) 860
BssT1I CCWWGG 2 cut(s) 21, 91
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 3 cut(s) 59, 654, 822
BstACI GRCGYC 1 cut(s) 860
BstDEI CTNAG 3 cut(s) 203, 327, 369
BstDSI CCRYGG 2 cut(s) 21, 91
BstF5I GGATG 6 cut(s) 157, 213, 217, 634, 887, 974
BstFNI CGCG 1 cut(s) 962
BstH2I RGCGCY 1 cut(s) 863
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 3 cut(s) 358, 523, 903
BstMBI GATC 3 cut(s) 355, 520, 900
BstMWI GCNNNNNNNGC 5 cut(s) 10, 38, 164, 425, 935
BstNI CCWGG 1 cut(s) 490
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 2 cut(s) 488, 792
BstSFI CTRYAG 1 cut(s) 188
BstUI CGCG 1 cut(s) 962
BstV1I GCAGC 3 cut(s) 25, 464, 922
BstV2I GAAGAC 1 cut(s) 357
Bsu36I CCTNAGG 1 cut(s) 203
BsuRI GGCC 2 cut(s) 90, 379
BtgI CCRYGG 2 cut(s) 21, 91
BtgZI GCGATG 2 cut(s) 729, 943
BtsCI GGATG 6 cut(s) 157, 213, 217, 634, 887, 974
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 3 cut(s) 111, 365, 831
CfoI GCGC 1 cut(s) 862
Cfr13I GGNCC 2 cut(s) 377, 398
CseI GACGC 1 cut(s) 951
Csp6I GTAC 1 cut(s) 414
CviAII CATG 7 cut(s) 22, 35, 92, 350, 763, 809, 892
CviQI GTAC 1 cut(s) 414
DdeI CTNAG 3 cut(s) 203, 327, 369
DinI GGCGCC 1 cut(s) 861
DpnI GATC 3 cut(s) 357, 522, 902
DpnII GATC 3 cut(s) 355, 520, 900
DriI GACNNNNNGTC 1 cut(s) 763
EaeI YGGCCR 1 cut(s) 88
Eam1105I GACNNNNNGTC 1 cut(s) 763
EciI GGCGGA 1 cut(s) 960
Eco130I CCWWGG 2 cut(s) 21, 91
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 2 cut(s) 581, 915
Eco81I CCTNAGG 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 377
EcoRI GAATTC 2 cut(s) 555, 635
EcoRII CCWGG 1 cut(s) 488
EcoT14I CCWWGG 2 cut(s) 21, 91
EgeI GGCGCC 1 cut(s) 861
EheI GGCGCC 1 cut(s) 861
ErhI CCWWGG 2 cut(s) 21, 91
FaeI CATG 7 cut(s) 25, 38, 95, 353, 766, 812, 895
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FaqI GGGAC 1 cut(s) 770
FatI CATG 7 cut(s) 21, 34, 91, 349, 762, 808, 891
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 4 cut(s) 39, 478, 936, 996
FokI GGATG 6 cut(s) 164, 220, 224, 641, 894, 961
Fsp4HI GCNGC 4 cut(s) 39, 478, 936, 996
FspBI CTAG 1 cut(s) 164
GlaI GCGC 1 cut(s) 861
GluI GCNGC 4 cut(s) 39, 478, 936, 996
GsuI CTGGAG 1 cut(s) 259
HaeII RGCGCY 1 cut(s) 863
HaeIII GGCC 2 cut(s) 90, 379
HapII CCGG 2 cut(s) 408, 793
HgaI GACGC 1 cut(s) 951
HhaI GCGC 1 cut(s) 862
Hin1I GRCGYC 1 cut(s) 860
Hin1II CATG 7 cut(s) 25, 38, 95, 353, 766, 812, 895
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HincII GTYRAC 1 cut(s) 773
HindII GTYRAC 1 cut(s) 773
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 1 cut(s) 470
HpaII CCGG 2 cut(s) 408, 793
HphI GGTGA 3 cut(s) 182, 479, 509
Hpy166II GTNNAC 4 cut(s) 227, 773, 784, 818
Hpy188I TCNGA 5 cut(s) 370, 388, 437, 561, 979
Hpy188III TCNNGA 4 cut(s) 47, 205, 408, 689
Hpy8I GTNNAC 4 cut(s) 227, 773, 784, 818
HpyAV CCTTC 5 cut(s) 287, 487, 556, 582, 976
HpyCH4III ACNGT 3 cut(s) 59, 654, 822
HpyCH4V TGCA 5 cut(s) 4, 190, 338, 762, 938
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 38, 164, 425, 935
HpyF3I CTNAG 3 cut(s) 203, 327, 369
Hsp92I GRCGYC 1 cut(s) 860
Hsp92II CATG 7 cut(s) 25, 38, 95, 353, 766, 812, 895
HspAI GCGC 1 cut(s) 860
KasI GGCGCC 1 cut(s) 859
Kpn2I TCCGGA 1 cut(s) 407
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 3 cut(s) 355, 520, 900
Lsp1109I GCAGC 3 cut(s) 25, 464, 922
LweI GCATC 4 cut(s) 19, 210, 619, 947
MaeI CTAG 1 cut(s) 164
MaeIII GTNAC 2 cut(s) 676, 884
MalI GATC 3 cut(s) 357, 522, 902
MboI GATC 3 cut(s) 355, 520, 900
MboII GAAGA 2 cut(s) 38, 357
MfeI CAATTG 1 cut(s) 621
MhlI GDGCHC 1 cut(s) 147
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 8 cut(s) 112, 245, 403, 508, 555, 621, 635, 800
MluNI TGGCCA 1 cut(s) 90
Mly113I GGCGCC 1 cut(s) 860
MmeI TCCRAC 1 cut(s) 565
MnlI CCTC 8 cut(s) 89, 212, 227, 364, 367, 390, 493, 871
Mox20I TGGCCA 1 cut(s) 90
MroI TCCGGA 1 cut(s) 407
MroXI GAANNNNTTC 2 cut(s) 197, 555
MscI TGGCCA 1 cut(s) 90
MseI TTAA 2 cut(s) 803, 915
MslI CAYNNNNRTG 1 cut(s) 896
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 935
MspI CCGG 2 cut(s) 408, 793
MspR9I CCNGG 2 cut(s) 490, 794
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 1 cut(s) 490
MvnI CGCG 1 cut(s) 962
MwoI GCNNNNNNNGC 5 cut(s) 10, 38, 164, 425, 935
NarI GGCGCC 1 cut(s) 860
NciI CCSGG 1 cut(s) 794
NcoI CCATGG 2 cut(s) 21, 91
NdeII GATC 3 cut(s) 355, 520, 900
NlaIII CATG 7 cut(s) 25, 38, 95, 353, 766, 812, 895
NlaIV GGNNCC 3 cut(s) 378, 861, 950
NmuCI GTSAC 2 cut(s) 676, 884
NspI RCATGY 1 cut(s) 766
PctI GAATGC 1 cut(s) 338
PdmI GAANNNNTTC 2 cut(s) 197, 555
PfeI GAWTC 1 cut(s) 470
PflFI GACNNNGTC 1 cut(s) 788
PflMI CCANNNNNTGG 1 cut(s) 869
PfoI TCCNGGA 2 cut(s) 488, 792
PkrI GCNGC 4 cut(s) 40, 479, 937, 997
PluTI GGCGCC 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 488
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 3 cut(s) 378, 861, 950
PspPI GGNCC 2 cut(s) 377, 398
PstI CTGCAG 1 cut(s) 192
PsyI GACNNNGTC 1 cut(s) 788
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 1 cut(s) 415
RsaNI GTAC 1 cut(s) 414
RseI CAYNNNNRTG 1 cut(s) 896
SaqAI TTAA 2 cut(s) 803, 915
SatI GCNGC 4 cut(s) 39, 478, 936, 996
Sau3AI GATC 3 cut(s) 355, 520, 900
Sau96I GGNCC 2 cut(s) 377, 398
ScrFI CCNGG 2 cut(s) 490, 794
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 4 cut(s) 19, 210, 619, 947
SfcI CTRYAG 1 cut(s) 188
SfoI GGCGCC 1 cut(s) 861
SinI GGWCC 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 896
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 8 cut(s) 112, 245, 403, 508, 555, 621, 635, 800
SsiI CCGC 3 cut(s) 632, 971, 993
SspDI GGCGCC 1 cut(s) 859
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 488, 792
StyI CCWWGG 2 cut(s) 21, 91
TaaI ACNGT 3 cut(s) 59, 654, 822
TasI AATT 8 cut(s) 112, 245, 403, 508, 555, 621, 635, 800
TatI WGTACW 1 cut(s) 413
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 2 cut(s) 803, 915
Tru9I TTAA 2 cut(s) 803, 915
TscAI CASTG 3 cut(s) 111, 365, 831
TseFI GTSAC 2 cut(s) 676, 884
TseI GCWGC 4 cut(s) 38, 477, 935, 995
Tsp45I GTSAC 2 cut(s) 676, 884
TspDTI ATGAA 3 cut(s) 168, 289, 825
TspRI CASTG 3 cut(s) 111, 365, 831
Tth111I GACNNNGTC 1 cut(s) 788
Van91I CCANNNNNTGG 1 cut(s) 869
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 4 cut(s) 112, 403, 555, 635
XceI RCATGY 1 cut(s) 766
XmnI GAANNNNTTC 2 cut(s) 197, 555
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.