Rorug06G0287200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000743
Physical Location & Seq
Forward (+)
43723321 .. 43724942
1622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0287200.1

Sequence Viewer

Length: 1407 bp
ATGTTGCTACAGAATGAACGAGTAGGAGAGGATCCTATTCGTCAATCAGTGCACCGCAGGCCTCCTCGATATTCATCCATTGAAGAAAGATCAGAATCAAAATTAATTCCTATGAATATTGATGATCTGCCTGGCCTTGTATTGGTTGAGATTCTTTGTAGACTTCCTTGCTATAAACTTGTTTTTCAGTCCAAGTGTGTCTCCAAGCGTTGGTGCGCTCTCATCTCAGATCCTTACTTTATTGGCCGCTTTATATGCCATCTCCGAAGTAGTGATATGGAAGCGCCACCAATAATCCGTACTCTGATAAACCGCAAAGGGGAGGAACTGTCATGGTCACCATCGTGGTGGTCGTCCAATTTGCTAGCACCGGTATTCAGAAAACTCATGAGTTTCCATGGATTGAAATATGAGCCAATTGTGAAAGCGACTTATAATGACTTGGTTTTGTGCTGCGAAGACGGGATGGACTTCTACTACATTTGCAATCCATCCACTGTGGAATGGGTTGCTCTTCCTCCCTCTCCTAGGTCCTATACTTGGCCACAGAGGAAAGCACCAGCAGCAATCATCTGTGATCGTAAGTACTATGATTACACGAAGGAAGGAAGAGGACATATCACCATCCTGCTTAATGCTGATTATAGGTGCAAGGTTGTCAGAATACTTCCATTTGTAGGAGAGGATCCACATTATCAACCCTCCAGGTTTACTGTGGAGATCTTTTCTTCTGAGTCTGGTGACTGGAAAGAGTCGGTCGTAGCTTCCCCTGTACCTTTGAATTTGAGCACAAGCCGTATTAGCTTTGCTTACAATGGAATGTTGTATTGGTGGGATTCTTTTACCAACCGATATGTTGGGATGGATCCTTTTATGATCAACAGTAATGGTAATGGTGATCATTATAAACTTCGTTTCATGAAATTTGATGATGAAGTTTTAGATCACCTTGGCACCATTGACTGCTTTGGTGTGTGCGGAGGGTATCTACGTATGTGTTACTACCAGGAGGCAGACGATACTCTATATGTTTGGGACTGGAAAGAAGAACAAGATTGTGATCAGAATGAAGCTGGCAGCAGTGAATTGTGTTGGAAACAGACTGGAGTCTATTCACTGGGATTGAGAATGGCTTCTGTTGGGGAATTGTTAGCTTTTGATCCAAATAATGATGACTTGTTGTATCTTGATGTTGATGAAGAAATTTGCCTGTGCAACATTCGAACAGGAAATTTGTCAGAGCTCGTTGGAAAGAGTCACGTTAATGTTAATGGCGATTTCTTTCCATTAGTGCTCCCATGGTGGCCGACACCAGTACCTAGACTACTAAAGCCTTCATGGCTCTCAGAGTTGATGAAGAAGTTGAGGATAGTAGCTAACTATTTAGTTTTCACTTGCTTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

468

Amino Acids

54.18

Weight (kDa)

5.44

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 139 - 413 3.3e-14 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 435, 906
AccB1I GGYRCC 1 cut(s) 953
AccB7I CCANNNNNTGG 1 cut(s) 210
AccI GTMKAC 1 cut(s) 160
AciI CCGC 4 cut(s) 55, 247, 313, 978
AclWI GGATC 8 cut(s) 26, 39, 224, 680, 693, 860, 873, 1154
AcoI YGGCCR 3 cut(s) 244, 542, 1304
AcsI RAATTY 4 cut(s) 781, 923, 1203, 1231
AfaI GTAC 4 cut(s) 301, 587, 774, 1317
AfiI CCNNNNNNNGG 6 cut(s) 142, 210, 319, 528, 540, 677
AgeI ACCGGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 83, 406, 781
AjnI CCWGG 3 cut(s) 130, 704, 1005
AjuI GAANNNNNNNTTGG 2 cut(s) 811, 843
AleI CACNNNNGTG 1 cut(s) 343
AluBI AGCT 6 cut(s) 764, 804, 1073, 1154, 1243, 1376
AluI AGCT 6 cut(s) 764, 804, 1073, 1154, 1243, 1376
Alw21I GWGCWC 4 cut(s) 54, 791, 1245, 1296
Alw26I GTCTC 1 cut(s) 205
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 8 cut(s) 26, 39, 224, 680, 693, 860, 873, 1154
AoxI GGCC 5 cut(s) 59, 133, 244, 542, 1304
ApaLI GTGCAC 1 cut(s) 50
ApeKI GCWGC 3 cut(s) 453, 563, 1077
ApoI RAATTY 4 cut(s) 781, 923, 1203, 1231
AseI ATTAAT 1 cut(s) 104
AsiGI ACCGGT 1 cut(s) 370
Asp700I GAANNNNTTC 1 cut(s) 1132
AspA2I CCTAGG 1 cut(s) 527
AspLEI GCGC 2 cut(s) 218, 286
AspS9I GGNCC 1 cut(s) 531
AsuHPI GGTGA 5 cut(s) 330, 613, 752, 908, 938
AsuII TTCGAA 1 cut(s) 1222
AsuNHI GCTAGC 1 cut(s) 364
AvaII GGWCC 1 cut(s) 531
AvrII CCTAGG 1 cut(s) 527
BaeGI GKGCMC 1 cut(s) 54
BaeI ACNNNNGTAYC 2 cut(s) 1299, 1332
BalI TGGCCA 1 cut(s) 544
BamHI GGATCC 3 cut(s) 31, 685, 865
BanI GGYRCC 1 cut(s) 953
BanII GRGCYC 1 cut(s) 1245
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 4 cut(s) 54, 791, 1245, 1296
BbvI GCAGC 3 cut(s) 440, 575, 1089
BccI CCATC 6 cut(s) 267, 349, 460, 499, 632, 856
BceAI ACGGC 1 cut(s) 780
BciT130I CCWGG 3 cut(s) 132, 706, 1007
BclI TGATCA 3 cut(s) 876, 898, 1060
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 3 cut(s) 365, 528, 1320
BfmI CTRYAG 1 cut(s) 8
BfoI RGCGCY 1 cut(s) 287
BglI GCCNNNNNGGC 1 cut(s) 1339
BglII AGATCT 1 cut(s) 720
BisI GCNGC 4 cut(s) 247, 454, 564, 1078
BlnI CCTAGG 1 cut(s) 527
BlsI GCNGC 4 cut(s) 248, 455, 565, 1079
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 3 cut(s) 132, 706, 1007
Bme18I GGWCC 1 cut(s) 531
BmgT120I GGNCC 1 cut(s) 531
BmiI GGNNCC 4 cut(s) 33, 687, 867, 955
BmrFI CCNGG 3 cut(s) 132, 706, 1007
BmrI ACTGGG 1 cut(s) 1127
BmtI GCTAGC 1 cut(s) 368
BmuI ACTGGG 1 cut(s) 1127
BoxI GACNNNNGTC 1 cut(s) 1106
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 2 cut(s) 688, 1125
Bpu14I TTCGAA 1 cut(s) 1222
BsaAI YACGTR 1 cut(s) 992
BsaBI GATNNNNATC 3 cut(s) 73, 94, 1059
BsaJI CCNNGG 4 cut(s) 397, 527, 949, 1298
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 6 cut(s) 142, 210, 319, 528, 540, 677
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 5 cut(s) 749, 1043, 1108, 1122, 1313
Bse8I GATNNNNATC 3 cut(s) 73, 94, 1059
BseBI CCWGG 3 cut(s) 132, 706, 1007
BseDI CCNNGG 4 cut(s) 397, 527, 949, 1298
BseGI GGATG 5 cut(s) 74, 471, 491, 624, 867
BseJI GATNNNNATC 3 cut(s) 73, 94, 1059
BseLI CCNNNNNNNGG 6 cut(s) 142, 210, 319, 528, 540, 677
BseMII CTCAG 3 cut(s) 240, 723, 1359
BseNI ACTGG 5 cut(s) 749, 1043, 1108, 1122, 1313
BseRI GAGGAG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 3 cut(s) 440, 575, 1089
Bsh1285I CGRYCG 1 cut(s) 759
BshFI GGCC 5 cut(s) 61, 135, 246, 544, 1306
BshNI GGYRCC 1 cut(s) 953
BshTI ACCGGT 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 759
BsiHKAI GWGCWC 4 cut(s) 54, 791, 1245, 1296
BsiSI CCGG 1 cut(s) 371
BslFI GGGAC 1 cut(s) 1049
BslI CCNNNNNNNGG 6 cut(s) 142, 210, 319, 528, 540, 677
BsmAI GTCTC 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 1049
BsnI GGCC 5 cut(s) 61, 135, 246, 544, 1306
Bsp119I TTCGAA 1 cut(s) 1222
Bsp1286I GDGCHC 4 cut(s) 54, 791, 1245, 1296
Bsp19I CCATGG 2 cut(s) 397, 1298
BspACI CCGC 4 cut(s) 55, 247, 313, 978
BspANI GGCC 5 cut(s) 61, 135, 246, 544, 1306
BspCNI CTCAG 3 cut(s) 239, 724, 1358
BspHI TCATGA 2 cut(s) 387, 918
BspLI GGNNCC 4 cut(s) 33, 687, 867, 955
BspOI GCTAGC 1 cut(s) 368
BspPI GGATC 8 cut(s) 26, 39, 224, 680, 693, 860, 873, 1154
BspQI GCTCTTC 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 1222
BspT107I GGYRCC 1 cut(s) 953
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 5 cut(s) 749, 1043, 1108, 1122, 1313
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 4 cut(s) 397, 527, 949, 1298
BssT1I CCWWGG 4 cut(s) 397, 527, 949, 1298
Bst2UI CCWGG 3 cut(s) 132, 706, 1007
Bst4CI ACNGT 4 cut(s) 330, 499, 715, 884
Bst6I CTCTTC 2 cut(s) 519, 604
BstBAI YACGTR 1 cut(s) 992
BstBI TTCGAA 1 cut(s) 1222
BstC8I GCNNGC 3 cut(s) 59, 366, 1075
BstDEI CTNAG 3 cut(s) 226, 732, 1345
BstDSI CCRYGG 2 cut(s) 397, 1298
BstEII GGTNACC 1 cut(s) 336
BstENI CCTNNNNNAGG 1 cut(s) 526
BstF5I GGATG 5 cut(s) 74, 471, 491, 624, 867
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 2 cut(s) 218, 286
BstMAI GTCTC 1 cut(s) 205
BstMCI CGRYCG 1 cut(s) 759
BstMWI GCNNNNNNNGC 5 cut(s) 58, 255, 563, 801, 1339
BstNI CCWGG 3 cut(s) 132, 706, 1007
BstPAI GACNNNNGTC 1 cut(s) 1106
BstPI GGTNACC 1 cut(s) 336
BstSCI CCNGG 3 cut(s) 130, 704, 1005
BstSFI CTRYAG 1 cut(s) 8
BstSLI GKGCMC 1 cut(s) 54
BstSNI TACGTA 1 cut(s) 992
BstV1I GCAGC 3 cut(s) 440, 575, 1089
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 5 cut(s) 31, 229, 685, 720, 865
BstXI CCANNNNNNTGG 1 cut(s) 348
BstYI RGATCY 5 cut(s) 31, 229, 685, 720, 865
BsuRI GGCC 5 cut(s) 61, 135, 246, 544, 1306
BtgI CCRYGG 2 cut(s) 397, 1298
BtsCI GGATG 5 cut(s) 74, 471, 491, 624, 867
BtsI GCAGTG 1 cut(s) 1087
BtsIMutI CAGTG 4 cut(s) 54, 495, 1087, 1115
Cac8I GCNNGC 3 cut(s) 59, 366, 1075
CciI TCATGA 2 cut(s) 387, 918
CfoI GCGC 2 cut(s) 218, 286
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 1 cut(s) 531
Csp6I GTAC 4 cut(s) 300, 586, 773, 1316
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 6 cut(s) 333, 388, 398, 919, 1299, 1338
CviQI GTAC 4 cut(s) 300, 586, 773, 1316
DdeI CTNAG 3 cut(s) 226, 732, 1345
EaeI YGGCCR 3 cut(s) 244, 542, 1304
Eam1104I CTCTTC 2 cut(s) 519, 604
EarI CTCTTC 2 cut(s) 519, 604
Ecl136II GAGCTC 1 cut(s) 1243
Eco105I TACGTA 1 cut(s) 992
Eco130I CCWWGG 4 cut(s) 397, 527, 949, 1298
Eco147I AGGCCT 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 1245
Eco47I GGWCC 1 cut(s) 531
Eco53kI GAGCTC 1 cut(s) 1243
Eco91I GGTNACC 1 cut(s) 336
EcoICRI GAGCTC 1 cut(s) 1243
EcoNI CCTNNNNNAGG 1 cut(s) 526
EcoO109I RGGNCCY 1 cut(s) 531
EcoO65I GGTNACC 1 cut(s) 336
EcoRII CCWGG 3 cut(s) 130, 704, 1005
EcoT14I CCWWGG 4 cut(s) 397, 527, 949, 1298
EcoT38I GRGCYC 1 cut(s) 1245
ErhI CCWWGG 4 cut(s) 397, 527, 949, 1298
FaeI CATG 6 cut(s) 336, 391, 401, 922, 1302, 1341
FaqI GGGAC 1 cut(s) 1049
FatI CATG 6 cut(s) 332, 387, 397, 918, 1298, 1337
FbaI TGATCA 3 cut(s) 876, 898, 1060
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 4 cut(s) 247, 454, 564, 1078
FokI GGATG 5 cut(s) 61, 478, 478, 611, 874
FriOI GRGCYC 1 cut(s) 1245
Fsp4HI GCNGC 4 cut(s) 247, 454, 564, 1078
FspBI CTAG 3 cut(s) 365, 528, 1320
GlaI GCGC 2 cut(s) 217, 285
GluI GCNGC 4 cut(s) 247, 454, 564, 1078
GsuI CTGGAG 2 cut(s) 688, 1125
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 5 cut(s) 61, 135, 246, 544, 1306
HapII CCGG 1 cut(s) 371
HhaI GCGC 2 cut(s) 218, 286
Hin1II CATG 6 cut(s) 336, 391, 401, 922, 1302, 1341
Hin6I GCGC 2 cut(s) 216, 284
HinP1I GCGC 2 cut(s) 216, 284
HinfI GANTC 7 cut(s) 95, 151, 734, 752, 836, 1107, 1255
HpaII CCGG 1 cut(s) 371
HphI GGTGA 5 cut(s) 330, 613, 752, 908, 938
Hpy166II GTNNAC 3 cut(s) 52, 161, 711
Hpy188III TCNNGA 3 cut(s) 388, 919, 1187
Hpy8I GTNNAC 3 cut(s) 52, 161, 711
HpyAV CCTTC 3 cut(s) 595, 599, 1344
HpyCH4III ACNGT 4 cut(s) 330, 499, 715, 884
HpyCH4IV ACGT 2 cut(s) 991, 1260
HpyCH4V TGCA 4 cut(s) 52, 486, 651, 1215
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 255, 563, 801, 1339
HpyF3I CTNAG 3 cut(s) 226, 732, 1345
HpySE526I ACGT 2 cut(s) 991, 1260
Hsp92II CATG 6 cut(s) 336, 391, 401, 922, 1302, 1341
HspAI GCGC 2 cut(s) 216, 284
Ksp22I TGATCA 3 cut(s) 876, 898, 1060
LguI GCTCTTC 1 cut(s) 519
LmnI GCTCC 1 cut(s) 1299
Lsp1109I GCAGC 3 cut(s) 440, 575, 1089
MaeI CTAG 3 cut(s) 365, 528, 1320
MaeII ACGT 2 cut(s) 991, 1260
MaeIII GTNAC 4 cut(s) 336, 740, 998, 1256
MboII GAAGA 8 cut(s) 95, 470, 506, 621, 720, 1058, 1211, 1369
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 5 cut(s) 31, 229, 685, 720, 865
MhlI GDGCHC 4 cut(s) 54, 791, 1245, 1296
MlsI TGGCCA 1 cut(s) 544
MluNI TGGCCA 1 cut(s) 544
MlyI GAGTC 4 cut(s) 743, 761, 1116, 1264
MmeI TCCRAC 2 cut(s) 1073, 1228
Mox20I TGGCCA 1 cut(s) 544
MroXI GAANNNNTTC 1 cut(s) 1132
MscI TGGCCA 1 cut(s) 544
MseI TTAA 4 cut(s) 104, 633, 1263, 1269
MslI CAYNNNNRTG 3 cut(s) 343, 346, 1263
Msp20I TGGCCA 1 cut(s) 544
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 3 cut(s) 132, 706, 1007
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 3 cut(s) 132, 706, 1007
MwoI GCNNNNNNNGC 5 cut(s) 58, 255, 563, 801, 1339
NcoI CCATGG 2 cut(s) 397, 1298
NheI GCTAGC 1 cut(s) 364
NlaIII CATG 6 cut(s) 336, 391, 401, 922, 1302, 1341
NlaIV GGNNCC 4 cut(s) 33, 687, 867, 955
NmuCI GTSAC 3 cut(s) 336, 740, 1256
NspV TTCGAA 1 cut(s) 1222
OliI CACNNNNGTG 1 cut(s) 343
PagI TCATGA 2 cut(s) 387, 918
PceI AGGCCT 1 cut(s) 61
PciSI GCTCTTC 1 cut(s) 519
PcsI WCGNNNNNNNCGW 1 cut(s) 350
PdmI GAANNNNTTC 1 cut(s) 1132
PfeI GAWTC 3 cut(s) 95, 151, 836
PflMI CCANNNNNTGG 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 4 cut(s) 248, 455, 565, 1079
PleI GAGTC 4 cut(s) 742, 760, 1115, 1263
PpsI GAGTC 4 cut(s) 742, 760, 1115, 1263
Ppu21I YACGTR 1 cut(s) 992
PpuMI RGGWCCY 1 cut(s) 531
PshAI GACNNNNGTC 1 cut(s) 1106
PshBI ATTAAT 1 cut(s) 104
PsiI TTATAA 2 cut(s) 435, 906
Psp124BI GAGCTC 1 cut(s) 1245
Psp5II RGGWCCY 1 cut(s) 531
Psp6I CCWGG 3 cut(s) 130, 704, 1005
PspEI GGTNACC 1 cut(s) 336
PspGI CCWGG 3 cut(s) 130, 704, 1005
PspN4I GGNNCC 4 cut(s) 33, 687, 867, 955
PspPI GGNCC 1 cut(s) 531
PspPPI RGGWCCY 1 cut(s) 531
PsuI RGATCY 5 cut(s) 31, 229, 685, 720, 865
RsaI GTAC 4 cut(s) 301, 587, 774, 1317
RsaNI GTAC 4 cut(s) 300, 586, 773, 1316
RseI CAYNNNNRTG 3 cut(s) 343, 346, 1263
SacI GAGCTC 1 cut(s) 1245
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 4 cut(s) 104, 633, 1263, 1269
SatI GCNGC 4 cut(s) 247, 454, 564, 1078
Sau96I GGNCC 1 cut(s) 531
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 4 cut(s) 743, 761, 1116, 1264
ScrFI CCNGG 3 cut(s) 132, 706, 1007
SduI GDGCHC 4 cut(s) 54, 791, 1245, 1296
SfcI CTRYAG 1 cut(s) 8
SfuI TTCGAA 1 cut(s) 1222
SinI GGWCC 1 cut(s) 531
SmiMI CAYNNNNRTG 3 cut(s) 343, 346, 1263
SnaBI TACGTA 1 cut(s) 992
SseBI AGGCCT 1 cut(s) 61
SsiI CCGC 4 cut(s) 55, 247, 313, 978
SspI AATATT 1 cut(s) 118
SspMI CTAG 3 cut(s) 365, 528, 1320
SstI GAGCTC 1 cut(s) 1245
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 3 cut(s) 130, 704, 1005
StyI CCWWGG 4 cut(s) 397, 527, 949, 1298
TaaI ACNGT 4 cut(s) 330, 499, 715, 884
TaiI ACGT 2 cut(s) 994, 1263
TaqI TCGA 2 cut(s) 67, 1222
TaqII GACCGA 1 cut(s) 745
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 3 cut(s) 95, 151, 836
Tru1I TTAA 4 cut(s) 104, 633, 1263, 1269
Tru9I TTAA 4 cut(s) 104, 633, 1263, 1269
TscAI CASTG 4 cut(s) 54, 502, 1087, 1122
TseFI GTSAC 3 cut(s) 336, 740, 1256
TseI GCWGC 3 cut(s) 453, 563, 1077
Tsp45I GTSAC 3 cut(s) 336, 740, 1256
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 4 cut(s) 54, 502, 1087, 1122
Van91I CCANNNNNTGG 1 cut(s) 210
VneI GTGCAC 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 531
VspI ATTAAT 1 cut(s) 104
XagI CCTNNNNNAGG 1 cut(s) 526
XapI RAATTY 4 cut(s) 781, 923, 1203, 1231
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XmaJI CCTAGG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 1132
XspI CTAG 3 cut(s) 365, 528, 1320
ZrmI AGTACT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.