Rorug02G0306200

protein phosphatase 2C 60

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000743
Physical Location & Seq
Forward (+)
34646321 .. 34648572
2252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0306200.1

Sequence Viewer

Length: 1494 bp
ATGGATACATTACTCAAAACACATAACAAGCTCGAGTTTCTGCACCCACTTCATGGGTTTTCAGAGAAAGTGAGTAATTGGAACTCTTCGAGGCAGGAGCTTCGATGTGGTCTCAGGAAGTCCTATAGAACTGGTCGTGTTAAGGCTAGTAGTAGTGCCCTTCTGGAGCTTGTGCCTGAAACCAAGAAGGAGAATCTCGAGTTTGAGCTTCCCTTGTACGACCCATCAAAGGGTCTTGTGGTGGACCTTGCAGTCGTGGGAGGCGGCCCTGCAGGGCTTGCGGTGGCGCAGCAAGTCTCGGAGGCAGGTCTCTCCGTTTGCTCGATTGATCCCTCTCCCAAGTTAATCTGGCCCAATAATTATGGTGTTTGGGTGGATGAGTTTGAGGCCATGGATTTGCTTGATTGCCTCGACACTACCTGGTCTGGTGCTGTTGTGTTCATAGATGAACAATCGAAGAAGGATCTTCATAGGCCATATGGGAGGGTTAATAGGAAGCAGCTCAAGTCCAAAATGCTGCAGAAATGCATTTCAAATGGTGTCAAGTTTCATCAAGCTAAAGTCAATAACGTTATTCACGAGGAGGAAAAATCACTCTTGATTTGCAATGATGGCGTCACAATTCAAGCTTCTGTGGTTCTTGATGCAACAGGTTTTTCAAGATGCCTTGTACAGTATGATAAGCCTTACAATCCAGGTTACCAAGTGGCATATGGGATTTTGGCTGAGGTTGAAGAACATCCATTTGATGTGGATAAGATGGTATTCATGGATTGGAGAGATTCGCATTTGAAGAATAGTCCTGAACTGAAAGAGAGAAATAGTAAGATCCCTACTTTTCTATATGCAATGCCTTTTTCATCCAACAGGATATTTCTTGAAGAAACCTCCCTCGTTGCTCGGCCTGGACTACCCATGAAAGATATCCAGGAAAGGATGGTTGCTAGATTGAGGCACCTAGGCATAAAAGTTAAGAGCATTGAAGAGGATGAGCATTGTGTCATTCCGATGGGCGGGCCCCTCCCAGTGCTCCCTCAAAGAGTTGTTGGAATTGGCGGTACAGCTGGGATGGTGCACCCTTCAACAGGATATATGGTTGCGAGGACTCTGGCAGCAGCTCCTATTGTTGCAAATGCCATAGTTCAGTACCTTGGTTCTGATAGTAGTCTTTCAGGAAATGAACTTTCAGCAGAAGTTTGGAAAGATTTATGGCCCATACAGAGGAGGAGACAAAGGGAGTTCTTCTGTTTCGGCATGTCCATTCTGCTCAAGCTGGATTTGAATGCCACAAGAAGGTTTTTCAATGCATTTTTTGATCTAGAACCTCGTTATTGGCATGGATTCTTGTCATCTCGACTGTTTCTTCCCGATCTTGTATTTTTCGGGCTTTCACTGTTTTCTCATGCTTCAAATGCTAGTAGATTAGAAATAATGGGAAAGGGAACTATTCCTTTGGTAAAGATGATCAACAACTTGATACAGGATAGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

497

Amino Acids

55.91

Weight (kDa)

7.63

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lycopene_cycl PF05834 82 - 474 5.2e-149 Lycopene cyclase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 954
AccB7I CCANNNNNTGG 1 cut(s) 53
AciI CCGC 4 cut(s) 264, 281, 1014, 1056
AclI AACGTT 1 cut(s) 570
AclWI GGATC 3 cut(s) 323, 471, 823
AcyI GRCGYC 1 cut(s) 615
AfaI GTAC 4 cut(s) 218, 672, 1060, 1148
AfiI CCNNNNNNNGG 7 cut(s) 53, 229, 230, 1013, 1085, 1221, 1293
AjnI CCWGG 4 cut(s) 419, 694, 904, 927
Alw21I GWGCWC 2 cut(s) 1032, 1077
Alw26I GTCTC 4 cut(s) 116, 301, 314, 1222
Alw44I GTGCAC 1 cut(s) 1073
AlwI GGATC 3 cut(s) 323, 471, 823
Ama87I CYCGRG 2 cut(s) 32, 197
AoxI GGCC 7 cut(s) 265, 350, 387, 473, 902, 1016, 1211
ApaI GGGCCC 1 cut(s) 1020
ApaLI GTGCAC 1 cut(s) 1073
ApeKI GCWGC 5 cut(s) 289, 499, 517, 1112, 1115
AspA2I CCTAGG 1 cut(s) 958
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
AvaI CYCGRG 2 cut(s) 32, 197
AvaII GGWCC 1 cut(s) 244
AvrII CCTAGG 1 cut(s) 958
BaeGI GKGCMC 3 cut(s) 160, 1020, 1077
BanI GGYRCC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 1020
BauI CACGAG 1 cut(s) 578
Bbv12I GWGCWC 2 cut(s) 1032, 1077
BbvCI CCTCAGC 1 cut(s) 726
BbvI GCAGC 5 cut(s) 301, 504, 511, 1124, 1127
BccI CCATC 6 cut(s) 232, 605, 754, 931, 1003, 1063
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 4 cut(s) 421, 696, 906, 929
BclI TGATCA 1 cut(s) 1464
BcoDI GTCTC 4 cut(s) 116, 301, 314, 1222
BfaI CTAG 5 cut(s) 147, 945, 959, 1319, 1416
BfmI CTRYAG 3 cut(s) 124, 270, 518
BfuAI ACCTGC 1 cut(s) 296
BisI GCNGC 6 cut(s) 265, 290, 500, 518, 1113, 1116
BlnI CCTAGG 1 cut(s) 958
BlsI GCNGC 6 cut(s) 266, 291, 501, 519, 1114, 1117
Bme1390I CCNGG 4 cut(s) 421, 696, 906, 929
Bme18I GGWCC 1 cut(s) 244
BmeT110I CYCGRG 2 cut(s) 32, 197
BmgT120I GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
BmiI GGNNCC 3 cut(s) 956, 1018, 1019
BmrFI CCNGG 4 cut(s) 421, 696, 906, 929
BmrI ACTGGG 1 cut(s) 1019
BmsI GCATC 2 cut(s) 634, 653
BmuI ACTGGG 1 cut(s) 1019
BpmI CTGGAG 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 726
BpuEI CTTGAG 2 cut(s) 488, 1253
BsaHI GRCGYC 1 cut(s) 615
BsaI GGTCTC 2 cut(s) 116, 314
BsaJI CCNNGG 3 cut(s) 390, 958, 1150
Bsc4I CCNNNNNNNGG 7 cut(s) 53, 229, 230, 1013, 1085, 1221, 1293
Bse1I ACTGG 2 cut(s) 136, 1025
Bse3DI GCAATG 2 cut(s) 613, 855
BseBI CCWGG 4 cut(s) 421, 696, 906, 929
BseDI CCNNGG 3 cut(s) 390, 958, 1150
BseGI GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BseLI CCNNNNNNNGG 7 cut(s) 53, 229, 230, 1013, 1085, 1221, 1293
BseMI GCAATG 2 cut(s) 613, 855
BseMII CTCAG 2 cut(s) 127, 717
BseNI ACTGG 2 cut(s) 136, 1025
BseRI GAGGAG 3 cut(s) 596, 1237, 1240
BseSI GKGCMC 3 cut(s) 160, 1020, 1077
BseXI GCAGC 5 cut(s) 301, 504, 511, 1124, 1127
BseYI CCCAGC 1 cut(s) 1064
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
BshNI GGYRCC 1 cut(s) 954
BsiHKAI GWGCWC 2 cut(s) 1032, 1077
BsiHKCI CYCGRG 2 cut(s) 32, 197
BslI CCNNNNNNNGG 7 cut(s) 53, 229, 230, 1013, 1085, 1221, 1293
BsmAI GTCTC 4 cut(s) 116, 301, 314, 1222
BsmI GAATGC 1 cut(s) 1288
BsnI GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
Bso31I GGTCTC 2 cut(s) 116, 314
BsoBI CYCGRG 2 cut(s) 32, 197
Bsp120I GGGCCC 1 cut(s) 1016
Bsp1286I GDGCHC 4 cut(s) 160, 1020, 1032, 1077
Bsp1407I TGTACA 1 cut(s) 670
Bsp143I GATC 6 cut(s) 328, 463, 828, 1315, 1369, 1464
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 4 cut(s) 264, 281, 1014, 1056
BspANI GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
BspCNI CTCAG 2 cut(s) 126, 718
BspLI GGNNCC 3 cut(s) 956, 1018, 1019
BspMAI CTGCAG 2 cut(s) 274, 522
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 3 cut(s) 323, 471, 823
BspT107I GGYRCC 1 cut(s) 954
BspTNI GGTCTC 2 cut(s) 116, 314
BsrDI GCAATG 2 cut(s) 613, 855
BsrGI TGTACA 1 cut(s) 670
BsrI ACTGG 2 cut(s) 136, 1025
BssECI CCNNGG 3 cut(s) 390, 958, 1150
BssMI GATC 6 cut(s) 328, 463, 828, 1315, 1369, 1464
BssNI GRCGYC 1 cut(s) 615
BssSI CACGAG 1 cut(s) 578
BssT1I CCWWGG 3 cut(s) 390, 958, 1150
Bst2BI CACGAG 1 cut(s) 578
Bst2UI CCWGG 4 cut(s) 421, 696, 906, 929
Bst4CI ACNGT 3 cut(s) 675, 1359, 1395
Bst6I CTCTTC 2 cut(s) 91, 978
BstACI GRCGYC 1 cut(s) 615
BstAPI GCANNNNNTGC 1 cut(s) 278
BstAUI TGTACA 1 cut(s) 670
BstC8I GCNNGC 2 cut(s) 279, 1016
BstDEI CTNAG 2 cut(s) 113, 726
BstDSI CCRYGG 1 cut(s) 390
BstEII GGTNACC 1 cut(s) 698
BstENI CCTNNNNNAGG 1 cut(s) 1083
BstF5I GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 6 cut(s) 331, 466, 831, 1318, 1372, 1467
BstMAI GTCTC 4 cut(s) 116, 301, 314, 1222
BstMBI GATC 6 cut(s) 328, 463, 828, 1315, 1369, 1464
BstMWI GCNNNNNNNGC 3 cut(s) 278, 612, 1412
BstNI CCWGG 4 cut(s) 421, 696, 906, 929
BstNSI RCATGY 1 cut(s) 1258
BstPI GGTNACC 1 cut(s) 698
BstSCI CCNGG 4 cut(s) 419, 694, 904, 927
BstSFI CTRYAG 3 cut(s) 124, 270, 518
BstSLI GKGCMC 3 cut(s) 160, 1020, 1077
BstV1I GCAGC 5 cut(s) 301, 504, 511, 1124, 1127
BstX2I RGATCY 2 cut(s) 463, 828
BstYI RGATCY 2 cut(s) 463, 828
BsuRI GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BtsIMutI CAGTG 2 cut(s) 1032, 1391
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 2 cut(s) 279, 1016
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
CseI GACGC 1 cut(s) 604
CsiI ACCWGGT 1 cut(s) 419
Csp6I GTAC 4 cut(s) 217, 671, 1059, 1147
CviAII CATG 7 cut(s) 53, 391, 769, 916, 1255, 1337, 1403
CviQI GTAC 4 cut(s) 217, 671, 1059, 1147
DdeI CTNAG 2 cut(s) 113, 726
DpnI GATC 6 cut(s) 330, 465, 830, 1317, 1371, 1466
DpnII GATC 6 cut(s) 328, 463, 828, 1315, 1369, 1464
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 2 cut(s) 91, 978
EarI CTCTTC 2 cut(s) 91, 978
Eco130I CCWWGG 3 cut(s) 390, 958, 1150
Eco24I GRGCYC 1 cut(s) 1020
Eco31I GGTCTC 2 cut(s) 116, 314
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 1 cut(s) 244
Eco88I CYCGRG 2 cut(s) 32, 197
Eco91I GGTNACC 1 cut(s) 698
EcoNI CCTNNNNNAGG 1 cut(s) 1083
EcoO109I RGGNCCY 1 cut(s) 1017
EcoO65I GGTNACC 1 cut(s) 698
EcoRII CCWGG 4 cut(s) 419, 694, 904, 927
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 3 cut(s) 390, 958, 1150
EcoT22I ATGCAT 2 cut(s) 530, 1309
EcoT38I GRGCYC 1 cut(s) 1020
ErhI CCWWGG 3 cut(s) 390, 958, 1150
FaeI CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
FatI CATG 7 cut(s) 52, 390, 768, 915, 1254, 1336, 1402
FauI CCCGC 1 cut(s) 1007
FauNDI CATATG 2 cut(s) 478, 712
FbaI TGATCA 1 cut(s) 1464
Fnu4HI GCNGC 6 cut(s) 265, 290, 500, 518, 1113, 1116
FokI GGATG 6 cut(s) 389, 726, 847, 949, 1001, 1081
FriOI GRGCYC 1 cut(s) 1020
Fsp4HI GCNGC 6 cut(s) 265, 290, 500, 518, 1113, 1116
FspBI CTAG 5 cut(s) 147, 945, 959, 1319, 1416
GlaI GCGC 1 cut(s) 288
GluI GCNGC 6 cut(s) 265, 290, 500, 518, 1113, 1116
GsaI CCCAGC 1 cut(s) 1068
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
HgaI GACGC 1 cut(s) 604
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 1 cut(s) 615
Hin1II CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 627
HinfI GANTC 4 cut(s) 193, 782, 1105, 1341
Hpy166II GTNNAC 2 cut(s) 244, 1075
Hpy188I TCNGA 4 cut(s) 64, 301, 1008, 1159
Hpy8I GTNNAC 2 cut(s) 244, 1075
HpyAV CCTTC 5 cut(s) 170, 181, 454, 1089, 1287
HpyCH4III ACNGT 3 cut(s) 675, 1359, 1395
HpyCH4IV ACGT 1 cut(s) 570
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 612, 1412
HpyF3I CTNAG 2 cut(s) 113, 726
HpySE526I ACGT 1 cut(s) 570
Hsp92I GRCGYC 1 cut(s) 615
Hsp92II CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
HspAI GCGC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 1464
Kzo9I GATC 6 cut(s) 328, 463, 828, 1315, 1369, 1464
LmnI GCTCC 4 cut(s) 97, 166, 1035, 1123
Lsp1109I GCAGC 5 cut(s) 301, 504, 511, 1124, 1127
LweI GCATC 2 cut(s) 634, 653
MabI ACCWGGT 1 cut(s) 419
MaeI CTAG 5 cut(s) 147, 945, 959, 1319, 1416
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 2 cut(s) 616, 698
MalI GATC 6 cut(s) 330, 465, 830, 1317, 1371, 1466
MboI GATC 6 cut(s) 328, 463, 828, 1315, 1369, 1464
MboII GAAGA 9 cut(s) 78, 458, 469, 746, 805, 893, 995, 1234, 1355
MflI RGATCY 2 cut(s) 463, 828
MhlI GDGCHC 4 cut(s) 160, 1020, 1032, 1077
MluCI AATT 4 cut(s) 76, 358, 621, 1050
MlyI GAGTC 1 cut(s) 1099
MmeI TCCRAC 2 cut(s) 888, 1027
Mph1103I ATGCAT 2 cut(s) 530, 1309
MseI TTAA 4 cut(s) 141, 344, 489, 972
MslI CAYNNNNRTG 1 cut(s) 1007
MspA1I CMGCKG 1 cut(s) 1064
MspR9I CCNGG 4 cut(s) 421, 696, 906, 929
Mva1269I GAATGC 1 cut(s) 1288
MvaI CCWGG 4 cut(s) 421, 696, 906, 929
MwoI GCNNNNNNNGC 3 cut(s) 278, 612, 1412
NcoI CCATGG 1 cut(s) 390
NdeI CATATG 2 cut(s) 478, 712
NdeII GATC 6 cut(s) 328, 463, 828, 1315, 1369, 1464
NlaIII CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
NlaIV GGNNCC 3 cut(s) 956, 1018, 1019
NmeAIII GCCGAG 1 cut(s) 880
NmuCI GTSAC 1 cut(s) 616
NsiI ATGCAT 2 cut(s) 530, 1309
NspI RCATGY 1 cut(s) 1258
PaeR7I CTCGAG 2 cut(s) 32, 197
PcsI WCGNNNNNNNCGW 1 cut(s) 576
PctI GAATGC 1 cut(s) 1288
PfeI GAWTC 3 cut(s) 193, 782, 1341
PflMI CCANNNNNTGG 1 cut(s) 53
PfoI TCCNGGA 1 cut(s) 927
PkrI GCNGC 6 cut(s) 266, 291, 501, 519, 1114, 1117
PleI GAGTC 1 cut(s) 1099
PpsI GAGTC 1 cut(s) 1099
Psp1406I AACGTT 1 cut(s) 570
Psp6I CCWGG 4 cut(s) 419, 694, 904, 927
PspEI GGTNACC 1 cut(s) 698
PspFI CCCAGC 1 cut(s) 1064
PspGI CCWGG 4 cut(s) 419, 694, 904, 927
PspN4I GGNNCC 3 cut(s) 956, 1018, 1019
PspOMI GGGCCC 1 cut(s) 1016
PspPI GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 2 cut(s) 274, 522
PsuI RGATCY 2 cut(s) 463, 828
PvuII CAGCTG 1 cut(s) 1064
RsaI GTAC 4 cut(s) 218, 672, 1060, 1148
RsaNI GTAC 4 cut(s) 217, 671, 1059, 1147
RseI CAYNNNNRTG 1 cut(s) 1007
SaqAI TTAA 4 cut(s) 141, 344, 489, 972
SatI GCNGC 6 cut(s) 265, 290, 500, 518, 1113, 1116
Sau3AI GATC 6 cut(s) 328, 463, 828, 1315, 1369, 1464
Sau96I GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
SbfI CCTGCAGG 1 cut(s) 274
SchI GAGTC 1 cut(s) 1099
ScrFI CCNGG 4 cut(s) 421, 696, 906, 929
SdaI CCTGCAGG 1 cut(s) 274
SduI GDGCHC 4 cut(s) 160, 1020, 1032, 1077
SexAI ACCWGGT 1 cut(s) 419
SfaNI GCATC 2 cut(s) 634, 653
SfcI CTRYAG 3 cut(s) 124, 270, 518
Sfr274I CTCGAG 2 cut(s) 32, 197
SinI GGWCC 1 cut(s) 244
SlaI CTCGAG 2 cut(s) 32, 197
SmiMI CAYNNNNRTG 1 cut(s) 1007
SmlI CTYRAG 4 cut(s) 32, 197, 503, 1268
SmoI CTYRAG 4 cut(s) 32, 197, 503, 1268
Sse8387I CCTGCAGG 1 cut(s) 274
Sse9I AATT 4 cut(s) 76, 358, 621, 1050
SsiI CCGC 4 cut(s) 264, 281, 1014, 1056
SspMI CTAG 5 cut(s) 147, 945, 959, 1319, 1416
StyD4I CCNGG 4 cut(s) 419, 694, 904, 927
StyI CCWWGG 3 cut(s) 390, 958, 1150
TaaI ACNGT 3 cut(s) 675, 1359, 1395
TaiI ACGT 1 cut(s) 573
TaqI TCGA 8 cut(s) 33, 89, 103, 198, 323, 411, 455, 1354
TasI AATT 4 cut(s) 76, 358, 621, 1050
TatI WGTACW 1 cut(s) 670
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 3 cut(s) 193, 782, 1341
Tru1I TTAA 4 cut(s) 141, 344, 489, 972
Tru9I TTAA 4 cut(s) 141, 344, 489, 972
TscAI CASTG 2 cut(s) 1032, 1398
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 5 cut(s) 289, 499, 517, 1112, 1115
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 9 cut(s) 41, 430, 458, 462, 539, 757, 849, 932, 1194
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 2 cut(s) 1032, 1398
Van91I CCANNNNNTGG 1 cut(s) 53
VneI GTGCAC 1 cut(s) 1073
VpaK11BI GGWCC 1 cut(s) 244
XagI CCTNNNNNAGG 1 cut(s) 1083
XbaI TCTAGA 1 cut(s) 1318
XceI RCATGY 1 cut(s) 1258
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XhoI CTCGAG 2 cut(s) 32, 197
XmaJI CCTAGG 1 cut(s) 958
XspI CTAG 5 cut(s) 147, 945, 959, 1319, 1416
Zsp2I ATGCAT 2 cut(s) 530, 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.