AT4G31860

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000743
Physical Location & Seq
Reverse (-)
15406439 .. 15409056
2618 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31860.1

Sequence Viewer

Length: 1074 bp
ATGGGTATATATCTAAGTACTCCAAAAACAGACAAGTTCTCAGAAGATGGCGAAAATCATAAACTCAGATATGGTTTATCCTCTATGCAAGGTTGGCGTGCGTCCATGGAAGATGCTCATGCTGCAATACTTGATCTCGATGATAACACTTCCTTCTTGGGTGTCTATGATGGTCATGGAGGTAAAGTTGTTTCTAAGTTCTGTGCCAAGTATCTACACCAGCAGGTTCTTAGTGATGAGGCGTATGCAGCTGGAGACGTAGGGACTTCTCTTCAAAAAGCATTTTTCAGAATGGATGAGATGATGCAAGGACAAAGAGGGTGGCGAGAGTTAGCAGTACTTGGTGACAAAATCAATAAGTTCAGTGGGATGATTGAAGGGCTTATATGGTCACCAAGAAGTGGGGACAGTGCTAATAAACCTGATGCTTGGGCGTTTGAGGAAGGTCCTCATTCTGATTTTGCTGGACCTAATTCTGGGAGCACGGCATGCGTAGCTGTTGTTAGAGACAAGCAGCTATTTGTTGCAAATGCAGGTGACTCACGTTGTGTGATATCCAGAAAGAATCAGGCTTATAATCTTTCTAGAGATCACAAACCAGATCTTGAAGCTGAGAAAGAAAGGATATTGAAAGCTGGTGGCTTTATACATGCAGGGCGAGTCAATGGAAGCTTAAATCTATCACGAGCTATCGGTGACATGGAATTCAAGCAGAATAAGTTTTTGCCATCTGAAAAGCAAATAGTTACCGCTAGTCCAGATGTTAACACTGTTGAACTCTGTGATGATGATGATTTCCTTGTTCTTGCCTGCGATGGAATTTGGGATTGCATGACAAGCCAACAACTCGTTGATTTCATACATGAACAATTGAATTCAGAGACCAAACTCTCGGTGGTATGTGAAAAAGTTCTCGATAGATGTCTGGCTCCAAACACTTCAGGTGGTGAAGGCTGTGATAACATGACCATGATATTGGTTCGATTCAAGAACCCTACTCCATCAGAGACCGAACTAAAACCAGAAGCAAGCCAGGCAGAAGGAAACCACGATGAGCCGAGCTCATCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

357

Amino Acids

39.2

Weight (kDa)

4.97

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 23 - 103 1.5e-10 Protein phosphatase 2C
PP2C PF00481 156 - 314 1.6e-61 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
AarI CACCTGC 1 cut(s) 524
Acc36I ACCTGC 2 cut(s) 214, 524
AccB7I CCANNNNNTGG 1 cut(s) 401
AciI CCGC 1 cut(s) 750
AcsI RAATTY 3 cut(s) 704, 819, 874
AcuI CTGAAG 1 cut(s) 924
AdeI CACNNNGTG 1 cut(s) 548
AfaI GTAC 2 cut(s) 19, 339
AfiI CCNNNNNNNGG 2 cut(s) 401, 476
AgsI TTSAA 8 cut(s) 275, 377, 608, 631, 709, 776, 874, 988
AjnI CCWGG 1 cut(s) 1032
AluBI AGCT 8 cut(s) 251, 497, 517, 611, 635, 672, 689, 1062
AluI AGCT 8 cut(s) 251, 497, 517, 611, 635, 672, 689, 1062
Alw21I GWGCWC 2 cut(s) 485, 1064
Alw26I GTCTC 4 cut(s) 249, 501, 875, 1001
ApeKI GCWGC 3 cut(s) 122, 248, 514
ApoI RAATTY 3 cut(s) 704, 819, 874
Asp700I GAANNNNTTC 1 cut(s) 909
AspS9I GGNCC 2 cut(s) 446, 467
AsuHPI GGTGA 5 cut(s) 356, 384, 548, 707, 959
AvaII GGWCC 2 cut(s) 446, 467
BanII GRGCYC 1 cut(s) 1064
BauI CACGAG 1 cut(s) 684
Bbv12I GWGCWC 2 cut(s) 485, 1064
BbvI GCAGC 3 cut(s) 109, 260, 526
BccI CCATC 5 cut(s) 41, 164, 736, 809, 1009
BceAI ACGGC 1 cut(s) 501
BciT130I CCWGG 1 cut(s) 1034
BcoDI GTCTC 4 cut(s) 249, 501, 875, 1001
BfaI CTAG 3 cut(s) 585, 753, 1072
BfuAI ACCTGC 2 cut(s) 214, 524
BglII AGATCT 1 cut(s) 601
BisI GCNGC 3 cut(s) 123, 249, 515
BlsI GCNGC 3 cut(s) 124, 250, 516
BmcAI AGTACT 2 cut(s) 19, 339
Bme1390I CCNGG 1 cut(s) 1034
Bme18I GGWCC 2 cut(s) 446, 467
BmgT120I GGNCC 2 cut(s) 446, 467
BmiI GGNNCC 1 cut(s) 930
BmrFI CCNGG 1 cut(s) 1034
BmsI GCATC 3 cut(s) 103, 294, 415
BplI GAGNNNNNCTC 1 cut(s) 1046
BpmI CTGGAG 1 cut(s) 273
BsaI GGTCTC 2 cut(s) 875, 1001
BsaJI CCNNGG 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 476
BseBI CCWGG 1 cut(s) 1034
BseDI CCNNGG 1 cut(s) 105
BseGI GGATG 2 cut(s) 301, 375
BseLI CCNNNNNNNGG 2 cut(s) 401, 476
BseMII CTCAG 3 cut(s) 54, 79, 603
BseXI GCAGC 3 cut(s) 109, 260, 526
BsiHKAI GWGCWC 2 cut(s) 485, 1064
BslFI GGGAC 2 cut(s) 277, 419
BslI CCNNNNNNNGG 2 cut(s) 401, 476
BsmAI GTCTC 4 cut(s) 249, 501, 875, 1001
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 2 cut(s) 277, 419
Bso31I GGTCTC 2 cut(s) 875, 1001
Bsp1286I GDGCHC 2 cut(s) 485, 1064
Bsp143I GATC 3 cut(s) 133, 589, 601
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 1 cut(s) 750
BspCNI CTCAG 3 cut(s) 53, 78, 604
BspLI GGNNCC 1 cut(s) 930
BspMI ACCTGC 2 cut(s) 214, 524
BspTNI GGTCTC 2 cut(s) 875, 1001
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 3 cut(s) 133, 589, 601
BssSI CACGAG 1 cut(s) 684
BssT1I CCWWGG 1 cut(s) 105
Bst2BI CACGAG 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 1034
Bst4CI ACNGT 2 cut(s) 410, 772
Bst6I CTCTTC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 99, 490, 811, 1030
BstDEI CTNAG 6 cut(s) 14, 40, 65, 195, 230, 612
BstDSI CCRYGG 1 cut(s) 105
BstEII GGTNACC 1 cut(s) 390
BstF5I GGATG 2 cut(s) 301, 375
BstKTI GATC 3 cut(s) 136, 592, 604
BstMAI GTCTC 4 cut(s) 249, 501, 875, 1001
BstMBI GATC 3 cut(s) 133, 589, 601
BstMWI GCNNNNNNNGC 7 cut(s) 94, 122, 248, 489, 494, 837, 1034
BstNI CCWGG 1 cut(s) 1034
BstNSI RCATGY 2 cut(s) 492, 653
BstPI GGTNACC 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 1032
BstV1I GCAGC 3 cut(s) 109, 260, 526
BstX2I RGATCY 1 cut(s) 601
BstXI CCANNNNNNTGG 1 cut(s) 976
BstYI RGATCY 1 cut(s) 601
BtgI CCRYGG 1 cut(s) 105
BtgZI GCGATG 1 cut(s) 828
BtsCI GGATG 2 cut(s) 301, 375
BtsIMutI CAGTG 3 cut(s) 370, 415, 768
BveI ACCTGC 2 cut(s) 214, 524
Cac8I GCNNGC 4 cut(s) 99, 490, 811, 1030
Cfr13I GGNCC 2 cut(s) 446, 467
CseI GACGC 1 cut(s) 90
Csp6I GTAC 2 cut(s) 18, 338
CspCI CAANNNNNGTGG 2 cut(s) 302, 337
CviQI GTAC 2 cut(s) 18, 338
DdeI CTNAG 6 cut(s) 14, 40, 65, 195, 230, 612
DpnI GATC 3 cut(s) 135, 591, 603
DpnII GATC 3 cut(s) 133, 589, 601
DraIII CACNNNGTG 1 cut(s) 548
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 1062
Eco130I CCWWGG 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 1064
Eco31I GGTCTC 2 cut(s) 875, 1001
Eco32I GATATC 1 cut(s) 555
Eco47I GGWCC 2 cut(s) 446, 467
Eco53kI GAGCTC 1 cut(s) 1062
Eco57I CTGAAG 1 cut(s) 924
Eco91I GGTNACC 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 1062
EcoO109I RGGNCCY 1 cut(s) 446
EcoO65I GGTNACC 1 cut(s) 390
EcoRI GAATTC 2 cut(s) 704, 874
EcoRII CCWGG 1 cut(s) 1032
EcoRV GATATC 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 1064
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 249
FaqI GGGAC 2 cut(s) 277, 419
Fnu4HI GCNGC 3 cut(s) 123, 249, 515
FokI GGATG 2 cut(s) 308, 382
FriOI GRGCYC 1 cut(s) 1064
Fsp4HI GCNGC 3 cut(s) 123, 249, 515
FspBI CTAG 3 cut(s) 585, 753, 1072
GluI GCNGC 3 cut(s) 123, 249, 515
GsuI CTGGAG 1 cut(s) 273
HgaI GACGC 1 cut(s) 90
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 4 cut(s) 539, 565, 660, 984
HpaI GTTAAC 1 cut(s) 766
HphI GGTGA 5 cut(s) 356, 384, 548, 707, 959
Hpy166II GTNNAC 1 cut(s) 766
Hpy188I TCNGA 7 cut(s) 43, 68, 290, 457, 733, 880, 1006
Hpy188III TCNNGA 8 cut(s) 137, 558, 585, 605, 684, 758, 914, 988
Hpy8I GTNNAC 1 cut(s) 766
HpyAV CCTTC 5 cut(s) 163, 371, 437, 944, 1034
HpyCH4III ACNGT 2 cut(s) 410, 772
HpyCH4IV ACGT 2 cut(s) 258, 544
HpyCH4V TGCA 8 cut(s) 88, 125, 248, 307, 527, 533, 653, 831
HpyF10VI GCNNNNNNNGC 7 cut(s) 94, 122, 248, 489, 494, 837, 1034
HpyF3I CTNAG 6 cut(s) 14, 40, 65, 195, 230, 612
HpySE526I ACGT 2 cut(s) 258, 544
KspAI GTTAAC 1 cut(s) 766
Kzo9I GATC 3 cut(s) 133, 589, 601
LmnI GCTCC 2 cut(s) 480, 934
Lsp1109I GCAGC 3 cut(s) 109, 260, 526
LweI GCATC 3 cut(s) 103, 294, 415
MaeI CTAG 3 cut(s) 585, 753, 1072
MaeII ACGT 2 cut(s) 258, 544
MaeIII GTNAC 5 cut(s) 344, 390, 536, 695, 745
MalI GATC 3 cut(s) 135, 591, 603
MboI GATC 3 cut(s) 133, 589, 601
MboII GAAGA 3 cut(s) 56, 122, 263
MfeI CAATTG 1 cut(s) 869
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 2 cut(s) 485, 1064
MluCI AATT 5 cut(s) 472, 704, 819, 869, 874
MlyI GAGTC 2 cut(s) 533, 669
MnlI CCTC 6 cut(s) 91, 173, 232, 311, 433, 459
MroXI GAANNNNTTC 1 cut(s) 909
MseI TTAA 2 cut(s) 674, 765
MslI CAYNNNNRTG 1 cut(s) 968
MspA1I CMGCKG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 1034
MunI CAATTG 1 cut(s) 869
MvaI CCWGG 1 cut(s) 1034
MwoI GCNNNNNNNGC 7 cut(s) 94, 122, 248, 489, 494, 837, 1034
NcoI CCATGG 1 cut(s) 105
NdeII GATC 3 cut(s) 133, 589, 601
NlaIV GGNNCC 1 cut(s) 930
NmuCI GTSAC 4 cut(s) 344, 390, 536, 695
NspI RCATGY 2 cut(s) 492, 653
PaeI GCATGC 1 cut(s) 492
PaqCI CACCTGC 1 cut(s) 524
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 2 cut(s) 565, 984
PflMI CCANNNNNTGG 1 cut(s) 401
PkrI GCNGC 3 cut(s) 124, 250, 516
PleI GAGTC 2 cut(s) 533, 668
PpsI GAGTC 2 cut(s) 533, 668
PpuMI RGGWCCY 1 cut(s) 446
PsiI TTATAA 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 1064
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 1032
PspEI GGTNACC 1 cut(s) 390
PspGI CCWGG 1 cut(s) 1032
PspN4I GGNNCC 1 cut(s) 930
PspPI GGNCC 2 cut(s) 446, 467
PspPPI RGGWCCY 1 cut(s) 446
PsuI RGATCY 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 2 cut(s) 19, 339
RsaNI GTAC 2 cut(s) 18, 338
RseI CAYNNNNRTG 1 cut(s) 968
SacI GAGCTC 1 cut(s) 1064
SaqAI TTAA 2 cut(s) 674, 765
SatI GCNGC 3 cut(s) 123, 249, 515
Sau3AI GATC 3 cut(s) 133, 589, 601
Sau96I GGNCC 2 cut(s) 446, 467
ScaI AGTACT 2 cut(s) 19, 339
SchI GAGTC 2 cut(s) 533, 669
ScrFI CCNGG 1 cut(s) 1034
SduI GDGCHC 2 cut(s) 485, 1064
SfaNI GCATC 3 cut(s) 103, 294, 415
SinI GGWCC 2 cut(s) 446, 467
SmiMI CAYNNNNRTG 1 cut(s) 968
SphI GCATGC 1 cut(s) 492
Sse9I AATT 5 cut(s) 472, 704, 819, 869, 874
SsiI CCGC 1 cut(s) 750
SspMI CTAG 3 cut(s) 585, 753, 1072
SstI GAGCTC 1 cut(s) 1064
StyD4I CCNGG 1 cut(s) 1032
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 410, 772
TaiI ACGT 2 cut(s) 261, 547
TaqI TCGA 3 cut(s) 138, 915, 982
TaqII GACCGA 1 cut(s) 1025
TasI AATT 5 cut(s) 472, 704, 819, 869, 874
TatI WGTACW 2 cut(s) 17, 337
TfiI GAWTC 2 cut(s) 565, 984
Tru1I TTAA 2 cut(s) 674, 765
Tru9I TTAA 2 cut(s) 674, 765
TscAI CASTG 3 cut(s) 370, 415, 775
TseFI GTSAC 4 cut(s) 344, 390, 536, 695
TseI GCWGC 3 cut(s) 122, 248, 514
Tsp45I GTSAC 4 cut(s) 344, 390, 536, 695
TspDTI ATGAA 2 cut(s) 847, 879
TspRI CASTG 3 cut(s) 370, 415, 775
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 2 cut(s) 446, 467
XapI RAATTY 3 cut(s) 704, 819, 874
XbaI TCTAGA 1 cut(s) 584
XceI RCATGY 2 cut(s) 492, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 3 cut(s) 585, 753, 1072
ZrmI AGTACT 2 cut(s) 19, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.