Rw6G034900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000743
Physical Location & Seq
Forward (+)
59484858 .. 59486830
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034900.1

Sequence Viewer

Length: 1536 bp
ATGGCTCTTCTGAGCTTCAACTCCATTTGTTGCCTGTCGACTTCGCCGGATCTTCAAAAATGGAATAATATTATAAAGACCCAAGTGCTCGAGGGGAACGCCGGGCAGGCAATTCGGTCGTATGTAAAAATGCAAAGACTTGGTCTCTTTGCTGATAACTATACATTTCCGGTTTTGCTGAAAGCAGCAGGTAATGCTTCCTATTTGGGTATAGGGTTAGCACTTCACGGACAGATACTGAAAACTGGGTTTTGTGACCATCTTTTCGTCCAAACTGCTCTGCTGAAAATGTATTCTTCTCTTGGAATCGTAGGGGATGCCCGCAAAGCGTTTGATAAAATGCCTGTGAAAGATGTGGTAGCCTGGAACTCCATGTTATATGTGTATGCTTCTAGTGGGCAGATGGGGAATGCAGTGCAAGTCTTTGATACAATGCCATCACAGGACCTTTTGTCTTTCAATATTATGATCTCTGGGTTCGCAGGGACCGGAAGCAACATATCTGCCAGAAGCATTTTTGATAGAATTCCTGACAAGGACATTGTGTCTTGGAACTCAATGATATTGGCGTGCATGAACGCTGGAGATGTGGCTGAAGCGCATGAGTTGTTCGAGTCGATGCCTGAGAAAAATGTCATAACTTGGAACACAATGATAATGGGTTATCTGAACAATCAGGTTTACTCTAAGGCAATCGAATTCTTGGACAAAATGAAGATGGGAAATATCGAACCGGATCATCTGAGTGTGACTGGTGCTTTATCTGCCTGTGCTCATTTGGGGTCGCTTGAAACTGGCATAAAAATACATACATATGCCAAACTCTCCGGGCAGGCTTCAAGTCCCCATGTAGCAACAGCACTCATAGATATGTATGCCAAGTGCGGAAGCATACACAACTCTTTAGAGGTGTTCTACAAGTTCAAACAAAAAGATGTCTACTGTTGGAATGCCATAATTTCAGGACTTGCTCTTCACGGTTTTGGCGACGCAGCTCTGAAAATCTTCAACCAAATGAGAGCCGATTGTCGAGTAAAACCAGATGATATCACCTTCATTGGGGTTTTAAGTGCTTGCAGCCATGGAGGGTTGGTAGAACAAGGATGTGAATTGTTTGATTCAATGGAAAAGCAGTTCGGGATTACTCCAAAGGCAGAGCATTATGGGTGCATGGTCGACCTTCTGAGCAGAGCGGGTCTTTTCGACAGTGCTTTGACACTCATAGAGACAATGCCTTTTGAAGCAAGCGAGTCCATCTTGGGTGCTTTGCTGAGTGCTTGTGTGATTCATCAGGACCTTGAAAATGGGGAGAAAGCAATGAAGCTAATTGTTTCGACCAAGGCTGCTCGGAGCGATGGGGAGTACATGATGTTTGCAAACTTGTACGCGTCATGCGGTGAATGGGAAGAAGCACAGAGATGGAGAGATATGATGAATGAGTCCGGGATTGAAAAGACTGCAGGTTGCAGCTTAGTTGAGATTAATGGAAGGTTTCACAAGTTTATGGCTGGAGATGAGAATGGGATTGTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

511

Amino Acids

55.98

Weight (kDa)

5.57

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 117 - 161 1e-06 PPR repeat family
PPR_2 PF13041 179 - 212 3.2e-07 PPR repeat family
PPR PF01535 182 - 212 1.1e-06 PPR repeat
PPR_2 PF13041 210 - 259 1.8e-10 PPR repeat family
PPR_2 PF13041 311 - 360 2e-07 PPR repeat family
E_motif PF20431 430 - 493 5.2e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 74
Acc36I ACCTGC 2 cut(s) 179, 1451
AccBSI CCGCTC 1 cut(s) 1193
AccI GTMKAC 3 cut(s) 38, 939, 1176
AccII CGCG 1 cut(s) 1388
AciI CCGC 4 cut(s) 322, 885, 1193, 1395
AclWI GGATC 2 cut(s) 57, 744
AcsI RAATTY 2 cut(s) 525, 698
AcuI CTGAAG 1 cut(s) 615
AfaI GTAC 2 cut(s) 1364, 1385
AfiI CCNNNNNNNGG 1 cut(s) 1059
AflIII ACRYGT 1 cut(s) 1386
AhdI GACNNNNNGTC 2 cut(s) 451, 544
AjnI CCWGG 1 cut(s) 362
AloI GAACNNNNNNTCC 2 cut(s) 1118, 1150
AluBI AGCT 4 cut(s) 15, 995, 1324, 1470
AluI AGCT 4 cut(s) 15, 995, 1324, 1470
Alw21I GWGCWC 2 cut(s) 90, 775
Alw26I GTCTC 2 cut(s) 149, 1220
AlwI GGATC 2 cut(s) 57, 744
AlwNI CAGNNNCTG 1 cut(s) 238
Ama87I CYCGRG 1 cut(s) 89
ApeKI GCWGC 5 cut(s) 185, 992, 1077, 1343, 1467
ApoI RAATTY 2 cut(s) 525, 698
ArsI GACNNNNNNTTYG 4 cut(s) 127, 159, 248, 280
AseI ATTAAT 1 cut(s) 1482
Asp700I GAANNNNTTC 1 cut(s) 1004
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 3 cut(s) 445, 486, 1294
AsuC2I CCSGG 3 cut(s) 103, 829, 1444
AsuHPI GGTGA 2 cut(s) 1042, 1409
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 3 cut(s) 445, 486, 1294
Bbv12I GWGCWC 2 cut(s) 90, 775
BbvI GCAGC 5 cut(s) 197, 1004, 1089, 1330, 1479
BccI CCATC 7 cut(s) 267, 397, 445, 712, 1262, 1349, 1413
BcgI CGANNNNNNTGC 4 cut(s) 95, 99, 129, 133
BciT130I CCWGG 1 cut(s) 364
BcnI CCSGG 3 cut(s) 103, 829, 1444
BcoDI GTCTC 2 cut(s) 149, 1220
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 1 cut(s) 1458
BfuAI ACCTGC 2 cut(s) 179, 1451
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 5 cut(s) 186, 993, 1078, 1344, 1468
BlsI GCNGC 5 cut(s) 187, 994, 1079, 1345, 1469
Bme1390I CCNGG 4 cut(s) 103, 364, 829, 1444
Bme18I GGWCC 3 cut(s) 445, 486, 1294
BmeRI GACNNNNNGTC 2 cut(s) 451, 544
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 445, 486, 1294
BmiI GGNNCC 1 cut(s) 487
BmrFI CCNGG 4 cut(s) 103, 364, 829, 1444
BmrI ACTGGG 1 cut(s) 255
BmsI GCATC 2 cut(s) 307, 609
BmuI ACTGGG 1 cut(s) 255
BpmI CTGGAG 2 cut(s) 603, 1530
BpuMI CCSGG 3 cut(s) 103, 829, 1444
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 1081, 1338
BsaWI WCCGGW 3 cut(s) 169, 488, 733
BsaXI ACNNNNNCTCC 2 cut(s) 1077, 1107
Bsc4I CCNNNNNNNGG 1 cut(s) 1059
Bse1I ACTGG 3 cut(s) 250, 757, 799
Bse3DI GCAATG 1 cut(s) 1323
BseBI CCWGG 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 1081, 1338
BseGI GGATG 2 cut(s) 322, 1109
BseLI CCNNNNNNNGG 1 cut(s) 1059
BseMI GCAATG 1 cut(s) 1323
BseMII CTCAG 4 cut(s) 615, 734, 1175, 1262
BseNI ACTGG 3 cut(s) 250, 757, 799
BseXI GCAGC 5 cut(s) 197, 1004, 1089, 1330, 1479
Bsh1236I CGCG 1 cut(s) 1388
Bsh1285I CGRYCG 1 cut(s) 119
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 90, 775
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 7 cut(s) 47, 102, 170, 489, 734, 828, 1443
BslFI GGGAC 2 cut(s) 499, 828
BslI CCNNNNNNNGG 1 cut(s) 1059
BsmAI GTCTC 2 cut(s) 149, 1220
BsmFI GGGAC 2 cut(s) 499, 828
BsmI GAATGC 2 cut(s) 415, 955
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 90, 775
Bsp143I GATC 3 cut(s) 49, 468, 736
Bsp19I CCATGG 1 cut(s) 1081
BspACI CCGC 4 cut(s) 322, 885, 1193, 1395
BspCNI CTCAG 4 cut(s) 616, 735, 1176, 1263
BspFNI CGCG 1 cut(s) 1388
BspLI GGNNCC 1 cut(s) 487
BspMAI CTGCAG 1 cut(s) 1462
BspMI ACCTGC 2 cut(s) 179, 1451
BspPI GGATC 2 cut(s) 57, 744
BspQI GCTCTTC 2 cut(s) 12, 978
BspTNI GGTCTC 1 cut(s) 149
BsrBI CCGCTC 1 cut(s) 1193
BsrDI GCAATG 1 cut(s) 1323
BsrI ACTGG 3 cut(s) 250, 757, 799
BssECI CCNNGG 2 cut(s) 1081, 1338
BssMI GATC 3 cut(s) 49, 468, 736
BssT1I CCWWGG 2 cut(s) 1081, 1338
Bst2UI CCWGG 1 cut(s) 364
Bst4CI ACNGT 3 cut(s) 944, 980, 1208
Bst6I CTCTTC 2 cut(s) 12, 978
BstAPI GCANNNNNTGC 1 cut(s) 194
BstC8I GCNNGC 6 cut(s) 108, 322, 571, 834, 1075, 1246
BstDEI CTNAG 7 cut(s) 11, 624, 687, 743, 1184, 1271, 1471
BstDSI CCRYGG 1 cut(s) 1081
BstF5I GGATG 2 cut(s) 322, 1109
BstFNI CGCG 1 cut(s) 1388
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 3 cut(s) 52, 471, 739
BstMAI GTCTC 2 cut(s) 149, 1220
BstMBI GATC 3 cut(s) 49, 468, 736
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 4 cut(s) 107, 194, 326, 764
BstNI CCWGG 1 cut(s) 364
BstSCI CCNGG 4 cut(s) 101, 362, 827, 1442
BstSFI CTRYAG 1 cut(s) 1458
BstUI CGCG 1 cut(s) 1388
BstV1I GCAGC 5 cut(s) 197, 1004, 1089, 1330, 1479
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BtgI CCRYGG 1 cut(s) 1081
BtgZI GCGATG 1 cut(s) 1368
BtsCI GGATG 2 cut(s) 322, 1109
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 2 cut(s) 420, 1213
BveI ACCTGC 2 cut(s) 179, 1451
Cac8I GCNNGC 6 cut(s) 108, 322, 571, 834, 1075, 1246
CaiI CAGNNNCTG 1 cut(s) 238
CfoI GCGC 1 cut(s) 601
Cfr13I GGNCC 3 cut(s) 445, 486, 1294
CseI GACGC 2 cut(s) 998, 1377
Csp6I GTAC 2 cut(s) 1363, 1384
CviAII CATG 8 cut(s) 373, 574, 602, 848, 1082, 1171, 1366, 1392
CviQI GTAC 2 cut(s) 1363, 1384
DdeI CTNAG 7 cut(s) 11, 624, 687, 743, 1184, 1271, 1471
DpnI GATC 3 cut(s) 51, 470, 738
DpnII GATC 3 cut(s) 49, 468, 736
DriI GACNNNNNGTC 2 cut(s) 451, 544
Eam1104I CTCTTC 2 cut(s) 12, 978
Eam1105I GACNNNNNGTC 2 cut(s) 451, 544
EarI CTCTTC 2 cut(s) 12, 978
Eco130I CCWWGG 2 cut(s) 1081, 1338
Eco31I GGTCTC 1 cut(s) 149
Eco32I GATATC 1 cut(s) 1048
Eco47I GGWCC 3 cut(s) 445, 486, 1294
Eco57I CTGAAG 1 cut(s) 615
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 2 cut(s) 445, 1294
EcoRI GAATTC 2 cut(s) 525, 698
EcoRII CCWGG 1 cut(s) 362
EcoRV GATATC 1 cut(s) 1048
EcoT14I CCWWGG 2 cut(s) 1081, 1338
ErhI CCWWGG 2 cut(s) 1081, 1338
FaeI CATG 8 cut(s) 376, 577, 605, 851, 1085, 1174, 1369, 1395
FaqI GGGAC 2 cut(s) 499, 828
FatI CATG 8 cut(s) 372, 573, 601, 847, 1081, 1170, 1365, 1391
FauI CCCGC 2 cut(s) 329, 1186
FauNDI CATATG 1 cut(s) 814
FblI GTMKAC 3 cut(s) 38, 939, 1176
Fnu4HI GCNGC 5 cut(s) 186, 993, 1078, 1344, 1468
FokI GGATG 2 cut(s) 329, 1116
Fsp4HI GCNGC 5 cut(s) 186, 993, 1078, 1344, 1468
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 600
GluI GCNGC 5 cut(s) 186, 993, 1078, 1344, 1468
GsuI CTGGAG 2 cut(s) 603, 1530
HapII CCGG 7 cut(s) 47, 102, 170, 489, 734, 828, 1443
HgaI GACGC 2 cut(s) 998, 1377
HhaI GCGC 1 cut(s) 601
Hin1II CATG 8 cut(s) 376, 577, 605, 851, 1085, 1174, 1369, 1395
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HincII GTYRAC 2 cut(s) 39, 1177
HindII GTYRAC 2 cut(s) 39, 1177
HinfI GANTC 6 cut(s) 306, 614, 1118, 1250, 1285, 1439
HpaII CCGG 7 cut(s) 47, 102, 170, 489, 734, 828, 1443
HphI GGTGA 2 cut(s) 1042, 1409
Hpy166II GTNNAC 4 cut(s) 39, 682, 940, 1177
Hpy188I TCNGA 6 cut(s) 12, 669, 744, 999, 1185, 1350
Hpy188III TCNNGA 4 cut(s) 530, 963, 1138, 1292
Hpy8I GTNNAC 4 cut(s) 39, 682, 940, 1177
Hpy99I CGWCG 1 cut(s) 992
HpyAV CCTTC 3 cut(s) 1063, 1190, 1482
HpyCH4III ACNGT 3 cut(s) 944, 980, 1208
HpyCH4V TGCA 9 cut(s) 133, 413, 418, 573, 1077, 1170, 1376, 1460, 1467
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 194, 326, 764
HpyF3I CTNAG 7 cut(s) 11, 624, 687, 743, 1184, 1271, 1471
Hsp92II CATG 8 cut(s) 376, 577, 605, 851, 1085, 1174, 1369, 1395
HspAI GCGC 1 cut(s) 599
Kzo9I GATC 3 cut(s) 49, 468, 736
LguI GCTCTTC 2 cut(s) 12, 978
LmnI GCTCC 1 cut(s) 1350
Lsp1109I GCAGC 5 cut(s) 197, 1004, 1089, 1330, 1479
LweI GCATC 2 cut(s) 307, 609
MaeI CTAG 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 254, 748
MalI GATC 3 cut(s) 51, 470, 738
MbiI CCGCTC 1 cut(s) 1193
MboI GATC 3 cut(s) 49, 468, 736
MboII GAAGA 6 cut(s) 44, 288, 727, 965, 997, 1418
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 2 cut(s) 90, 775
MluCI AATT 6 cut(s) 111, 525, 698, 957, 1109, 1326
MluI ACGCGT 1 cut(s) 1386
MlyI GAGTC 3 cut(s) 623, 1259, 1448
MmeI TCCRAC 1 cut(s) 926
MnlI CCTC 3 cut(s) 85, 901, 1079
MroXI GAANNNNTTC 1 cut(s) 1004
MseI TTAA 2 cut(s) 1067, 1482
MslI CAYNNNNRTG 4 cut(s) 744, 813, 869, 1417
MspI CCGG 7 cut(s) 47, 102, 170, 489, 734, 828, 1443
MspR9I CCNGG 4 cut(s) 103, 364, 829, 1444
Mva1269I GAATGC 2 cut(s) 415, 955
MvaI CCWGG 1 cut(s) 364
MvnI CGCG 1 cut(s) 1388
MwoI GCNNNNNNNGC 4 cut(s) 107, 194, 326, 764
NciI CCSGG 3 cut(s) 103, 829, 1444
NcoI CCATGG 1 cut(s) 1081
NdeI CATATG 1 cut(s) 814
NdeII GATC 3 cut(s) 49, 468, 736
NlaIII CATG 8 cut(s) 376, 577, 605, 851, 1085, 1174, 1369, 1395
NlaIV GGNNCC 1 cut(s) 487
NmuCI GTSAC 2 cut(s) 254, 748
PaeR7I CTCGAG 1 cut(s) 89
PciSI GCTCTTC 2 cut(s) 12, 978
PcsI WCGNNNNNNNCGW 1 cut(s) 984
PctI GAATGC 2 cut(s) 415, 955
PdmI GAANNNNTTC 1 cut(s) 1004
PfeI GAWTC 3 cut(s) 306, 1118, 1285
PflFI GACNNNGTC 1 cut(s) 141
PfoI TCCNGGA 1 cut(s) 1442
PkrI GCNGC 5 cut(s) 187, 994, 1079, 1345, 1469
PleI GAGTC 3 cut(s) 622, 1258, 1447
PpsI GAGTC 3 cut(s) 622, 1258, 1447
PpuMI RGGWCCY 2 cut(s) 445, 1294
PshBI ATTAAT 1 cut(s) 1482
PsiI TTATAA 1 cut(s) 74
Psp5II RGGWCCY 2 cut(s) 445, 1294
Psp6I CCWGG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 362
PspN4I GGNNCC 1 cut(s) 487
PspPI GGNCC 3 cut(s) 445, 486, 1294
PspPPI RGGWCCY 2 cut(s) 445, 1294
PspXI VCTCGAGB 1 cut(s) 89
PstI CTGCAG 1 cut(s) 1462
PstNI CAGNNNCTG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 49
PsyI GACNNNGTC 1 cut(s) 141
RsaI GTAC 2 cut(s) 1364, 1385
RsaNI GTAC 2 cut(s) 1363, 1384
RseI CAYNNNNRTG 4 cut(s) 744, 813, 869, 1417
SalI GTCGAC 2 cut(s) 37, 1175
SapI GCTCTTC 2 cut(s) 12, 978
SaqAI TTAA 2 cut(s) 1067, 1482
SatI GCNGC 5 cut(s) 186, 993, 1078, 1344, 1468
Sau3AI GATC 3 cut(s) 49, 468, 736
Sau96I GGNCC 3 cut(s) 445, 486, 1294
SchI GAGTC 3 cut(s) 623, 1259, 1448
ScrFI CCNGG 4 cut(s) 103, 364, 829, 1444
SduI GDGCHC 2 cut(s) 90, 775
SfaNI GCATC 2 cut(s) 307, 609
SfcI CTRYAG 1 cut(s) 1458
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 3 cut(s) 445, 486, 1294
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 4 cut(s) 744, 813, 869, 1417
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 6 cut(s) 111, 525, 698, 957, 1109, 1326
SsiI CCGC 4 cut(s) 322, 885, 1193, 1395
SspI AATATT 2 cut(s) 70, 463
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 4 cut(s) 101, 362, 827, 1442
StyI CCWWGG 2 cut(s) 1081, 1338
TaaI ACNGT 3 cut(s) 944, 980, 1208
TaqII GACCGA 1 cut(s) 105
TasI AATT 6 cut(s) 111, 525, 698, 957, 1109, 1326
TatI WGTACW 1 cut(s) 1362
TfiI GAWTC 3 cut(s) 306, 1118, 1285
Tru1I TTAA 2 cut(s) 1067, 1482
Tru9I TTAA 2 cut(s) 1067, 1482
TscAI CASTG 2 cut(s) 420, 1213
TseFI GTSAC 2 cut(s) 254, 748
TseI GCWGC 5 cut(s) 185, 992, 1077, 1343, 1467
Tsp45I GTSAC 2 cut(s) 254, 748
TspDTI ATGAA 6 cut(s) 590, 728, 1045, 1277, 1334, 1448
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 2 cut(s) 420, 1213
Tth111I GACNNNGTC 1 cut(s) 141
VpaK11BI GGWCC 3 cut(s) 445, 486, 1294
VspI ATTAAT 1 cut(s) 1482
XapI RAATTY 2 cut(s) 525, 698
XhoI CTCGAG 1 cut(s) 89
XmiI GTMKAC 3 cut(s) 38, 939, 1176
XmnI GAANNNNTTC 1 cut(s) 1004
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.