MD15G1041900.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000743
Physical Location & Seq
Reverse (-)
2928357 .. 2933287
4931 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1041900.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGCAAGGATGGCGTGCCACAATGGAGGATGCTCATGCAGCATATCCTGATCTGGATGCATCCACCTCATTCTTTGGTGTTTATGATGGCCATGGAGGCAAGGTAGTTGCTAAGTTCTGTGCAAAATATCTGCACCAACAAGTGCTCAAGAATGAAGCATATGCGGCTGGAGACATAGGAACGTCTGTTCAAAAAGCTTTTTTCAGAATGGATGAAATGATGCGCGGACAAAGGGGTTGGAGAGAGTTAGCTGTTTTGGGAGATAAGATAAACAAGTTTACTGGCATGATAGAAGGGTTGATTTGGTCTCCGAGGAGCAGTGATAGTAACGATCAAGCTGATGATTGGGCTTTTGAGGAGGGGCCTCACTCTGATTTTACTGGTCCAACTTCTGGGTGTACAGCCTGTGTTGCAATTCTTCGAAACAAGCAACTTGTTGTGGCAAATGCTGGTGATTCACGCTGTGTGATATCAAGGAAGGGTCAGGCATATAATCTGTCAAGGGACCACAAACCCGATCTTGAGCTTGAGAAGGAGAGGATTTTAAAAGCTGGTGGTTTTATCCATGCAGGACGAGTCAATGGAAGTTTGAATCTTGCAAGAGCTATAGGTGACATGGAATTTAAGCAGAATAAATTTTTGCCAGCTGAAAAACAAATTGTAACTGCCAGTCCGGACATAAACACTGTATGTGTTGAGCTTTGCGATGACGACGAATTTATTGTGCTAGCATGTGATGGCATCTGGGATTGTATGTCTAGCCAGCAAGTGGTAGATTTTGTACATGAACAACTACTCTCGGAAAGCAAGCTATCTGTGGTGTGCGAGAGAGCCCTTGATAGGTGCTTGGCGCCATCGACTGCTGATGGTGAGGGATGCGATAACATGACCATGATCGTGGTGCAGTTCAAGAAGCCTGAACAGTCGACTGAATCCGAGGGTGGGCAATCCTCACCAACTGAAGTAGCAGCCGGAAGTTATTCAAAGGGGAAGTTATTCAAAGGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

337

Amino Acids

36.76

Weight (kDa)

5.13

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 1 - 76 3.3e-09 Protein phosphatase 2C
PP2C PF00481 112 - 289 2.7e-59 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 850
AccB7I CCANNNNNTGG 2 cut(s) 392, 769
AccI GTMKAC 1 cut(s) 926
AccII CGCG 1 cut(s) 225
AccIII TCCGGA 1 cut(s) 673
AciI CCGC 2 cut(s) 164, 225
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 3 cut(s) 620, 635, 716
AcuI CTGAAG 1 cut(s) 981
AcyI GRCGYC 1 cut(s) 851
AdeI CACNNNGTG 1 cut(s) 464
AfaI GTAC 2 cut(s) 400, 783
AfiI CCNNNNNNNGG 4 cut(s) 392, 769, 840, 942
AgsI TTSAA 5 cut(s) 191, 592, 910, 984, 1000
AloI GAACNNNNNNTCC 2 cut(s) 171, 203
AluBI AGCT 9 cut(s) 197, 251, 338, 526, 551, 605, 647, 700, 811
AluI AGCT 9 cut(s) 197, 251, 338, 526, 551, 605, 647, 700, 811
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 2 cut(s) 165, 312
Aor13HI TCCGGA 1 cut(s) 673
AoxI GGCC 2 cut(s) 88, 362
ApeKI GCWGC 2 cut(s) 38, 968
ApoI RAATTY 3 cut(s) 620, 635, 716
Asp700I GAANNNNTTC 2 cut(s) 979, 995
AspLEI GCGC 2 cut(s) 225, 853
AspS9I GGNCC 3 cut(s) 362, 383, 505
AsuHPI GGTGA 4 cut(s) 464, 623, 881, 945
AsuII TTCGAA 1 cut(s) 421
AsuNHI GCTAGC 1 cut(s) 727
AvaII GGWCC 2 cut(s) 383, 505
BalI TGGCCA 1 cut(s) 90
BanI GGYRCC 1 cut(s) 850
BanII GRGCYC 1 cut(s) 835
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 2 cut(s) 50, 980
BccI CCATC 5 cut(s) 3, 80, 731, 860, 862
BcoDI GTCTC 2 cut(s) 165, 312
BfaI CTAG 2 cut(s) 728, 759
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 854
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 3 cut(s) 39, 165, 969
BlsI GCNGC 3 cut(s) 40, 166, 970
Bme18I GGWCC 2 cut(s) 383, 505
BmgT120I GGNCC 3 cut(s) 362, 383, 505
BmiI GGNNCC 3 cut(s) 363, 506, 852
BmsI GCATC 6 cut(s) 19, 46, 68, 210, 750, 866
BmtI GCTAGC 1 cut(s) 731
BpmI CTGGAG 1 cut(s) 189
Bpu14I TTCGAA 1 cut(s) 421
BpuEI CTTGAG 3 cut(s) 131, 542, 548
BsaHI GRCGYC 1 cut(s) 851
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 3 cut(s) 91, 311, 936
BsaWI WCCGGW 1 cut(s) 673
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 4 cut(s) 392, 769, 840, 942
Bse1I ACTGG 3 cut(s) 286, 385, 669
BseAI TCCGGA 1 cut(s) 673
BseDI CCNNGG 3 cut(s) 91, 311, 936
BseGI GGATG 6 cut(s) 14, 34, 59, 61, 217, 881
BseLI CCNNNNNNNGG 4 cut(s) 392, 769, 840, 942
BseNI ACTGG 3 cut(s) 286, 385, 669
BseRI GAGGAG 2 cut(s) 328, 371
BseXI GCAGC 2 cut(s) 50, 980
BsgI GTGCAG 2 cut(s) 116, 923
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 2 cut(s) 90, 364
BshNI GGYRCC 1 cut(s) 850
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 2 cut(s) 674, 972
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 4 cut(s) 392, 769, 840, 942
BsmAI GTCTC 2 cut(s) 165, 312
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 2 cut(s) 90, 364
Bso31I GGTCTC 1 cut(s) 312
Bsp119I TTCGAA 1 cut(s) 421
Bsp1286I GDGCHC 2 cut(s) 147, 835
Bsp13I TCCGGA 1 cut(s) 673
Bsp1407I TGTACA 2 cut(s) 398, 781
Bsp143I GATC 4 cut(s) 49, 331, 517, 894
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 2 cut(s) 164, 225
BspANI GGCC 2 cut(s) 90, 364
BspEI TCCGGA 1 cut(s) 673
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 3 cut(s) 363, 506, 852
BspOI GCTAGC 1 cut(s) 731
BspT104I TTCGAA 1 cut(s) 421
BspT107I GGYRCC 1 cut(s) 850
BspTNI GGTCTC 1 cut(s) 312
BsrGI TGTACA 2 cut(s) 398, 781
BsrI ACTGG 3 cut(s) 286, 385, 669
BssECI CCNNGG 3 cut(s) 91, 311, 936
BssMI GATC 4 cut(s) 49, 331, 517, 894
BssNI GRCGYC 1 cut(s) 851
BssT1I CCWWGG 1 cut(s) 91
Bst4CI ACNGT 2 cut(s) 688, 924
BstACI GRCGYC 1 cut(s) 851
BstAUI TGTACA 2 cut(s) 398, 781
BstBI TTCGAA 1 cut(s) 421
BstC8I GCNNGC 5 cut(s) 15, 645, 729, 764, 809
BstDEI CTNAG 1 cut(s) 111
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 6 cut(s) 14, 34, 59, 61, 217, 881
BstFNI CGCG 1 cut(s) 225
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 2 cut(s) 225, 853
BstKTI GATC 4 cut(s) 52, 334, 520, 897
BstMAI GTCTC 2 cut(s) 165, 312
BstMBI GATC 4 cut(s) 49, 331, 517, 894
BstMWI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
BstNSI RCATGY 1 cut(s) 735
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 2 cut(s) 50, 980
BstXI CCANNNNNNTGG 1 cut(s) 898
BsuRI GGCC 2 cut(s) 90, 364
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 6 cut(s) 14, 34, 59, 61, 217, 881
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 325, 684
Cac8I GCNNGC 5 cut(s) 15, 645, 729, 764, 809
CfoI GCGC 2 cut(s) 225, 853
Cfr13I GGNCC 3 cut(s) 362, 383, 505
Csp6I GTAC 2 cut(s) 399, 782
CviAII CATG 9 cut(s) 35, 92, 286, 566, 616, 732, 785, 886, 892
CviQI GTAC 2 cut(s) 399, 782
DdeI CTNAG 1 cut(s) 111
DinI GGCGCC 1 cut(s) 852
DpnI GATC 4 cut(s) 51, 333, 519, 896
DpnII GATC 4 cut(s) 49, 331, 517, 894
DraI TTTAAA 1 cut(s) 546
DraIII CACNNNGTG 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 835
Eco31I GGTCTC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 383, 505
Eco57I CTGAAG 1 cut(s) 981
EcoO109I RGGNCCY 1 cut(s) 362
EcoRV GATATC 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 835
EgeI GGCGCC 1 cut(s) 852
EheI GGCGCC 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 9 cut(s) 38, 95, 289, 569, 619, 735, 788, 889, 895
FaqI GGGAC 1 cut(s) 518
FatI CATG 9 cut(s) 34, 91, 285, 565, 615, 731, 784, 885, 891
FauNDI CATATG 2 cut(s) 160, 1007
FblI GTMKAC 1 cut(s) 926
Fnu4HI GCNGC 3 cut(s) 39, 165, 969
FokI GGATG 6 cut(s) 21, 41, 46, 68, 224, 888
FriOI GRGCYC 1 cut(s) 835
Fsp4HI GCNGC 3 cut(s) 39, 165, 969
FspBI CTAG 2 cut(s) 728, 759
GlaI GCGC 2 cut(s) 224, 852
GluI GCNGC 3 cut(s) 39, 165, 969
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 2 cut(s) 90, 364
HapII CCGG 2 cut(s) 674, 972
HhaI GCGC 2 cut(s) 225, 853
Hin1I GRCGYC 1 cut(s) 851
Hin1II CATG 9 cut(s) 38, 95, 289, 569, 619, 735, 788, 889, 895
Hin6I GCGC 2 cut(s) 223, 851
HinP1I GCGC 2 cut(s) 223, 851
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 4 cut(s) 455, 576, 592, 932
HpaII CCGG 2 cut(s) 674, 972
HphI GGTGA 4 cut(s) 464, 623, 881, 945
Hpy166II GTNNAC 3 cut(s) 279, 399, 927
Hpy188I TCNGA 5 cut(s) 206, 312, 373, 802, 937
Hpy188III TCNNGA 6 cut(s) 47, 53, 148, 521, 674, 910
Hpy8I GTNNAC 3 cut(s) 279, 399, 927
Hpy99I CGWCG 1 cut(s) 716
HpyAV CCTTC 3 cut(s) 287, 472, 526
HpyCH4III ACNGT 2 cut(s) 688, 924
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 9 cut(s) 4, 38, 59, 122, 133, 413, 569, 599, 904
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
HpyF3I CTNAG 1 cut(s) 111
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 851
Hsp92II CATG 9 cut(s) 38, 95, 289, 569, 619, 735, 788, 889, 895
HspAI GCGC 2 cut(s) 223, 851
KasI GGCGCC 1 cut(s) 850
Kpn2I TCCGGA 1 cut(s) 673
Kzo9I GATC 4 cut(s) 49, 331, 517, 894
LmnI GCTCC 1 cut(s) 315
Lsp1109I GCAGC 2 cut(s) 50, 980
LweI GCATC 6 cut(s) 19, 46, 68, 210, 750, 866
MaeI CTAG 2 cut(s) 728, 759
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 3 cut(s) 326, 611, 661
MalI GATC 4 cut(s) 51, 333, 519, 896
MboI GATC 4 cut(s) 49, 331, 517, 894
MboII GAAGA 1 cut(s) 410
MhlI GDGCHC 2 cut(s) 147, 835
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 5 cut(s) 414, 620, 635, 657, 716
MluNI TGGCCA 1 cut(s) 90
Mly113I GGCGCC 1 cut(s) 851
MlyI GAGTC 1 cut(s) 585
MmeI TCCRAC 2 cut(s) 218, 410
Mox20I TGGCCA 1 cut(s) 90
Mph1103I ATGCAT 1 cut(s) 61
MroI TCCGGA 1 cut(s) 673
MroXI GAANNNNTTC 2 cut(s) 979, 995
MscI TGGCCA 1 cut(s) 90
MseI TTAA 2 cut(s) 545, 624
MslI CAYNNNNRTG 2 cut(s) 890, 896
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 2 cut(s) 674, 972
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
NarI GGCGCC 1 cut(s) 851
NcoI CCATGG 1 cut(s) 91
NdeI CATATG 2 cut(s) 160, 1007
NdeII GATC 4 cut(s) 49, 331, 517, 894
NheI GCTAGC 1 cut(s) 727
NlaIII CATG 9 cut(s) 38, 95, 289, 569, 619, 735, 788, 889, 895
NlaIV GGNNCC 3 cut(s) 363, 506, 852
NmuCI GTSAC 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 1 cut(s) 735
NspV TTCGAA 1 cut(s) 421
PdmI GAANNNNTTC 2 cut(s) 979, 995
PfeI GAWTC 3 cut(s) 455, 592, 932
PflMI CCANNNNNTGG 2 cut(s) 392, 769
PkrI GCNGC 3 cut(s) 40, 166, 970
PleI GAGTC 1 cut(s) 584
PluTI GGCGCC 1 cut(s) 854
PpsI GAGTC 1 cut(s) 584
PspN4I GGNNCC 3 cut(s) 363, 506, 852
PspPI GGNCC 3 cut(s) 362, 383, 505
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 2 cut(s) 400, 783
RsaNI GTAC 2 cut(s) 399, 782
RseI CAYNNNNRTG 2 cut(s) 890, 896
SalI GTCGAC 1 cut(s) 925
SaqAI TTAA 2 cut(s) 545, 624
SatI GCNGC 3 cut(s) 39, 165, 969
Sau3AI GATC 4 cut(s) 49, 331, 517, 894
Sau96I GGNCC 3 cut(s) 362, 383, 505
SchI GAGTC 1 cut(s) 585
SduI GDGCHC 2 cut(s) 147, 835
SfaNI GCATC 6 cut(s) 19, 46, 68, 210, 750, 866
SfcI CTRYAG 1 cut(s) 606
SfoI GGCGCC 1 cut(s) 852
SfuI TTCGAA 1 cut(s) 421
SinI GGWCC 2 cut(s) 383, 505
SmiMI CAYNNNNRTG 2 cut(s) 890, 896
SmlI CTYRAG 3 cut(s) 146, 521, 527
SmoI CTYRAG 3 cut(s) 146, 521, 527
Sse9I AATT 5 cut(s) 414, 620, 635, 657, 716
SsiI CCGC 2 cut(s) 164, 225
SspDI GGCGCC 1 cut(s) 850
SspMI CTAG 2 cut(s) 728, 759
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 2 cut(s) 688, 924
TaiI ACGT 1 cut(s) 185
TaqI TCGA 3 cut(s) 421, 857, 926
TasI AATT 5 cut(s) 414, 620, 635, 657, 716
TatI WGTACW 2 cut(s) 398, 781
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 3 cut(s) 455, 592, 932
Tru1I TTAA 2 cut(s) 545, 624
Tru9I TTAA 2 cut(s) 545, 624
TscAI CASTG 2 cut(s) 325, 691
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 2 cut(s) 38, 968
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 3 cut(s) 168, 228, 801
TspRI CASTG 2 cut(s) 325, 691
Van91I CCANNNNNTGG 2 cut(s) 392, 769
VpaK11BI GGWCC 2 cut(s) 383, 505
XapI RAATTY 3 cut(s) 620, 635, 716
XceI RCATGY 1 cut(s) 735
XmiI GTMKAC 1 cut(s) 926
XmnI GAANNNNTTC 2 cut(s) 979, 995
XspI CTAG 2 cut(s) 728, 759
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.