Prupe.3G100500_v2.0.a1

protein phosphatase 2C 60

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000743
Physical Location & Seq
Reverse (-)
7706230 .. 7713522
7293 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G100500.2

Sequence Viewer

Length: 1095 bp
ATGGGAATATATCTCAGTGCTCCCAAAACTGATAAGGTCTCAGAAGATGGTGAGAATGACAAACTTCGATACGGGTCATCCTCCATGCAAGGGTGGCGTTCGACCATGGAAGATGCTCATGCAGCTATTCCAGATTTTGACGGTTCAACATCTTTCTTTGCTGTTTATGATGGTCATGGAGGTAAAGCAGTGGCAAATTTCTGCGCCAAGTATCTCCACCAACAGGTGCTCAAGGATGAAGCATATCTAGCTGGCGATTTAGGCACTTCTCTACAGAAAGCTTTTCTCAGGATGGATGAGATGATGCGTGGACAAAGAGGCTGGAGAGAATTAGCCGTACTGGGAGATAAGATAGACAAAGTTTCTGGTCTTATAGAAGGATTCATTTTTTCTCCCAAGAGTGTTGAAGCTAAAAGTGGTGCATTTAATGACCATACTGATCAATGGTCTTCTGAAGAGGGGCCTCACTCTGATTTTGATGGACCGAATTCTGGAAGCACAGCTTGTGTTGCTATCATCCGAAACAATCAACTTGTTGTTGCCAATGCTGGTGATTCTCGTTGTGTTATATCCAGGAAGGGTCAGGCATATAATCTGTCAAAAGATCATAAGCCTGACATTGAGAATGAAAAAGAAAGAATTCTGAAGGCTGGTGGATTCATCCAAGTTGGAAGGGTCAATGGAAGTTTGAACTTGGCCAGAGCTATTGGGGATGCAGAATTCAAGCAGAACAAACATTTGCCTGTTGAAAGGCAGATCGTAACTGCCAATCCAGACGTAACTAGTGTTGAGATTTGTGATGATGATGAGTTTCTCGTCATAGCTTGTGATGGGATATGGGACTGCATGTCAAGCCAACAACTTGTGGACTACGTTCGGGAACAATTAAAACATGAAAGTAAACTCTCGGTGATTTGTGAGAGAGTGTTTGAAAGGTGCTTGGCACCATCATCTGGTGGAGAGGGGTGTGACAACATGACAATGATCTTGGTTCAGCTTAAGAAACCTGTTACTTCAGCTGCTTCTGTCGAGGACAAGCCTCTCTCGTCGAATCCATCATCTGAGAAGGATAAAAGCTCAGCAAAAGCTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

365

Amino Acids

39.76

Weight (kDa)

5.31

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 943
AccB7I CCANNNNNTGG 1 cut(s) 953
AcoI YGGCCR 1 cut(s) 696
AcsI RAATTY 4 cut(s) 196, 487, 639, 719
AcuI CTGAAG 3 cut(s) 474, 665, 999
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 4 cut(s) 90, 223, 491, 953
AflII CTTAAG 1 cut(s) 998
AgsI TTSAA 6 cut(s) 147, 407, 691, 724, 749, 932
AhdI GACNNNNNGTC 1 cut(s) 847
AhlI ACTAGT 1 cut(s) 782
AjnI CCWGG 1 cut(s) 572
Alw21I GWGCWC 2 cut(s) 22, 231
Alw26I GTCTC 1 cut(s) 43
AoxI GGCC 2 cut(s) 461, 696
ApeKI GCWGC 2 cut(s) 122, 1019
ApoI RAATTY 4 cut(s) 196, 487, 639, 719
Asp700I GAANNNNTTC 1 cut(s) 639
AspLEI GCGC 1 cut(s) 206
AspS9I GGNCC 2 cut(s) 461, 482
AsuHPI GGTGA 3 cut(s) 62, 563, 922
AvaII GGWCC 1 cut(s) 482
BalI TGGCCA 1 cut(s) 698
BanI GGYRCC 1 cut(s) 943
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 22, 231
BbvI GCAGC 2 cut(s) 134, 1006
BccI CCATC 7 cut(s) 41, 164, 286, 473, 824, 955, 1063
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 574
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 43
BcuI ACTAGT 1 cut(s) 782
BfaI CTAG 2 cut(s) 248, 783
BfmI CTRYAG 1 cut(s) 272
BfrI CTTAAG 1 cut(s) 998
BisI GCNGC 2 cut(s) 123, 1020
BlpI GCTNAGC 1 cut(s) 1078
BlsI GCNGC 2 cut(s) 124, 1021
Bme1390I CCNGG 1 cut(s) 574
Bme18I GGWCC 1 cut(s) 482
BmeRI GACNNNNNGTC 1 cut(s) 847
BmgT120I GGNCC 2 cut(s) 461, 482
BmiI GGNNCC 2 cut(s) 462, 945
BmrFI CCNGG 1 cut(s) 574
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 3 cut(s) 103, 294, 703
BmuI ACTGGG 1 cut(s) 350
BpiI GAAGAC 1 cut(s) 441
BpmI CTGGAG 1 cut(s) 343
Bpu1102I GCTNAGC 1 cut(s) 1078
BpuEI CTTGAG 1 cut(s) 215
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 1 cut(s) 105
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 223, 491, 953
Bse1I ACTGG 1 cut(s) 345
BseBI CCWGG 1 cut(s) 574
BseDI CCNNGG 1 cut(s) 105
BseGI GGATG 7 cut(s) 77, 241, 297, 301, 516, 660, 718
BseLI CCNNNNNNNGG 4 cut(s) 90, 223, 491, 953
BseMII CTCAG 5 cut(s) 28, 54, 301, 1053, 1092
BseNI ACTGG 1 cut(s) 345
BseXI GCAGC 2 cut(s) 134, 1006
BshFI GGCC 2 cut(s) 463, 698
BshNI GGYRCC 1 cut(s) 943
BsiHKAI GWGCWC 2 cut(s) 22, 231
BslFI GGGAC 1 cut(s) 854
BslI CCNNNNNNNGG 4 cut(s) 90, 223, 491, 953
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 854
BsnI GGCC 2 cut(s) 463, 698
Bso31I GGTCTC 1 cut(s) 43
Bsp1286I GDGCHC 2 cut(s) 22, 231
Bsp143I GATC 4 cut(s) 439, 604, 756, 984
Bsp1720I GCTNAGC 1 cut(s) 1078
Bsp19I CCATGG 1 cut(s) 105
BspANI GGCC 2 cut(s) 463, 698
BspCNI CTCAG 5 cut(s) 27, 53, 300, 1054, 1091
BspLI GGNNCC 2 cut(s) 462, 945
BspT107I GGYRCC 1 cut(s) 943
BspTI CTTAAG 1 cut(s) 998
BspTNI GGTCTC 1 cut(s) 43
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 4 cut(s) 439, 604, 756, 984
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 574
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 450
BstAFI CTTAAG 1 cut(s) 998
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 5 cut(s) 14, 40, 287, 1062, 1078
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 7 cut(s) 77, 241, 297, 301, 516, 660, 718
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 4 cut(s) 442, 607, 759, 987
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 4 cut(s) 439, 604, 756, 984
BstMWI GCNNNNNNNGC 6 cut(s) 94, 122, 248, 261, 509, 852
BstNI CCWGG 1 cut(s) 574
BstNSI RCATGY 1 cut(s) 850
BstSCI CCNGG 1 cut(s) 572
BstSFI CTRYAG 1 cut(s) 272
BstV1I GCAGC 2 cut(s) 134, 1006
BstV2I GAAGAC 1 cut(s) 441
BsuRI GGCC 2 cut(s) 463, 698
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 7 cut(s) 77, 241, 297, 301, 516, 660, 718
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 22, 195
Cac8I GCNNGC 1 cut(s) 253
CfoI GCGC 1 cut(s) 206
Cfr13I GGNCC 2 cut(s) 461, 482
Csp6I GTAC 1 cut(s) 338
CviAII CATG 7 cut(s) 85, 106, 119, 176, 847, 893, 976
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 5 cut(s) 14, 40, 287, 1062, 1078
DpnI GATC 4 cut(s) 441, 606, 758, 986
DpnII GATC 4 cut(s) 439, 604, 756, 984
DriI GACNNNNNGTC 1 cut(s) 847
EaeI YGGCCR 1 cut(s) 696
Eam1104I CTCTTC 1 cut(s) 450
Eam1105I GACNNNNNGTC 1 cut(s) 847
EarI CTCTTC 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 482
Eco57I CTGAAG 3 cut(s) 474, 665, 999
EcoO109I RGGNCCY 1 cut(s) 461
EcoRI GAATTC 3 cut(s) 487, 639, 719
EcoRII CCWGG 1 cut(s) 572
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 7 cut(s) 88, 109, 122, 179, 850, 896, 979
FalI AAGNNNNNCTT 2 cut(s) 487, 519
FaqI GGGAC 1 cut(s) 854
FatI CATG 7 cut(s) 84, 105, 118, 175, 846, 892, 975
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 2 cut(s) 123, 1020
FokI GGATG 7 cut(s) 64, 248, 304, 308, 503, 647, 725
Fsp4HI GCNGC 2 cut(s) 123, 1020
FspBI CTAG 2 cut(s) 248, 783
GlaI GCGC 1 cut(s) 205
GluI GCNGC 2 cut(s) 123, 1020
GsuI CTGGAG 1 cut(s) 343
HaeIII GGCC 2 cut(s) 463, 698
HhaI GCGC 1 cut(s) 206
Hin1II CATG 7 cut(s) 88, 109, 122, 179, 850, 896, 979
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 4 cut(s) 381, 554, 657, 1051
HphI GGTGA 3 cut(s) 62, 563, 922
Hpy166II GTNNAC 3 cut(s) 311, 868, 902
Hpy188I TCNGA 6 cut(s) 43, 454, 472, 521, 645, 1063
Hpy188III TCNNGA 5 cut(s) 131, 289, 492, 773, 878
Hpy8I GTNNAC 3 cut(s) 311, 868, 902
Hpy99I CGWCG 1 cut(s) 1051
HpyAV CCTTC 5 cut(s) 371, 571, 640, 666, 1060
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 2 cut(s) 777, 873
HpyCH4V TGCA 5 cut(s) 88, 122, 422, 716, 846
HpyF10VI GCNNNNNNNGC 6 cut(s) 94, 122, 248, 261, 509, 852
HpyF3I CTNAG 5 cut(s) 14, 40, 287, 1062, 1078
HpySE526I ACGT 2 cut(s) 777, 873
Hsp92II CATG 7 cut(s) 88, 109, 122, 179, 850, 896, 979
HspAI GCGC 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 4 cut(s) 439, 604, 756, 984
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 2 cut(s) 134, 1006
LweI GCATC 3 cut(s) 103, 294, 703
MaeI CTAG 2 cut(s) 248, 783
MaeII ACGT 2 cut(s) 777, 873
MaeIII GTNAC 4 cut(s) 760, 778, 968, 1009
MalI GATC 4 cut(s) 441, 606, 758, 986
MboI GATC 4 cut(s) 439, 604, 756, 984
MboII GAAGA 4 cut(s) 56, 122, 441, 467
MhlI GDGCHC 2 cut(s) 22, 231
MlsI TGGCCA 1 cut(s) 698
MluCI AATT 6 cut(s) 196, 329, 487, 639, 719, 884
MluNI TGGCCA 1 cut(s) 698
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 8 cut(s) 91, 173, 311, 451, 474, 955, 1024, 1050
Mox20I TGGCCA 1 cut(s) 698
MroXI GAANNNNTTC 1 cut(s) 639
MscI TGGCCA 1 cut(s) 698
MseI TTAA 3 cut(s) 426, 887, 999
MslI CAYNNNNRTG 1 cut(s) 980
Msp20I TGGCCA 1 cut(s) 698
MspA1I CMGCKG 1 cut(s) 1019
MspCI CTTAAG 1 cut(s) 998
MspR9I CCNGG 1 cut(s) 574
MvaI CCWGG 1 cut(s) 574
MwoI GCNNNNNNNGC 6 cut(s) 94, 122, 248, 261, 509, 852
NcoI CCATGG 1 cut(s) 105
NdeII GATC 4 cut(s) 439, 604, 756, 984
NlaIII CATG 7 cut(s) 88, 109, 122, 179, 850, 896, 979
NlaIV GGNNCC 2 cut(s) 462, 945
NmuCI GTSAC 1 cut(s) 968
NspI RCATGY 1 cut(s) 850
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 4 cut(s) 381, 554, 657, 1051
PflMI CCANNNNNTGG 1 cut(s) 953
PfoI TCCNGGA 1 cut(s) 572
PkrI GCNGC 2 cut(s) 124, 1021
Psp6I CCWGG 1 cut(s) 572
PspGI CCWGG 1 cut(s) 572
PspN4I GGNNCC 2 cut(s) 462, 945
PspPI GGNCC 2 cut(s) 461, 482
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
RseI CAYNNNNRTG 1 cut(s) 980
SaqAI TTAA 3 cut(s) 426, 887, 999
SatI GCNGC 2 cut(s) 123, 1020
Sau3AI GATC 4 cut(s) 439, 604, 756, 984
Sau96I GGNCC 2 cut(s) 461, 482
ScrFI CCNGG 1 cut(s) 574
SduI GDGCHC 2 cut(s) 22, 231
SfaNI GCATC 3 cut(s) 103, 294, 703
SfcI CTRYAG 1 cut(s) 272
SinI GGWCC 1 cut(s) 482
SmiMI CAYNNNNRTG 1 cut(s) 980
SmlI CTYRAG 2 cut(s) 230, 998
SmoI CTYRAG 2 cut(s) 230, 998
SpeI ACTAGT 1 cut(s) 782
Sse9I AATT 6 cut(s) 196, 329, 487, 639, 719, 884
SspMI CTAG 2 cut(s) 248, 783
StyD4I CCNGG 1 cut(s) 572
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 2 cut(s) 780, 876
TaqI TCGA 4 cut(s) 67, 101, 1029, 1049
TaqII GACCGA 1 cut(s) 499
TasI AATT 6 cut(s) 196, 329, 487, 639, 719, 884
TfiI GAWTC 4 cut(s) 381, 554, 657, 1051
Tru1I TTAA 3 cut(s) 426, 887, 999
Tru9I TTAA 3 cut(s) 426, 887, 999
TscAI CASTG 2 cut(s) 22, 195
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 2 cut(s) 122, 1019
Tsp45I GTSAC 1 cut(s) 968
TspDTI ATGAA 5 cut(s) 252, 373, 642, 649, 909
TspRI CASTG 2 cut(s) 22, 195
Van91I CCANNNNNTGG 1 cut(s) 953
Vha464I CTTAAG 1 cut(s) 998
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 4 cut(s) 196, 487, 639, 719
XceI RCATGY 1 cut(s) 850
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 2 cut(s) 248, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.