MD08G1055600.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000743
Physical Location & Seq
Forward (+)
4321611 .. 4326929
5319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1055600.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGCAAGGATGGCGTACCACAATGGAGGATGCTCATGCAGCATATCCTGATCTGGATGCATCCACCTCATTCTTTGGTGTTTATGATGGCCATGGAGGCAAGGTAGTTGCTAAGTTCTGTGCAAAATATCTTCACCAACAAGTACTCAAGAATGAAGCATATGCGGCTGGAGACATAGGAACTTCTGTTCAGAAAGCTTTTTTCAGAATGGATGAAATGATGCGTGGACAAAGGGGTTGGAGAGAGTTGGCTGTTTTGGGAGATAAGATAAACAAGTTTACTGGCATGATAGAAGGGTTAATTTGGTCTCCAAGGGGCAGTAATGGTAATGATCAAGCTGATGATTGGGCTTTTGAGGAGGGGCCTCACTCTGATTTTACTGGTCCAACTTCTGGGAGCACTGCCTGTGTTGCAATTCTTCGAAACAAGCAACTTTTTGTGGCGAATGCTGGTGATTCTCGTTGTGTGATATCAAGGAAGGGTCAGGCATATAATCTGTCAAGGGACCACAAACCTGATCTTGAGCTTGAGAAGGAGAGGATTTTAAAAGCTGGTGGTTTCATCCATGCAGGACGAGTCAATGGAAGTTTGAACCTTGCAAGAGCTATAGGTGACATGGAATTCAAGCAGAATAAATTTTTGCCAGATGAAAAACAAATCATAACTGCATGTCCGGACATAAACACTGTTGAGCTTTGTGATGACGATGAATTTATTGTGCTAGCATGCGATGGCATCTGGGACTGTATGTCTAGCCAGCAAGTGGTAGATTTTGTACGTGAACAACTACTTTCTGAAAGCAAGCTATCTGTGGTATGCGAGAGAGCCCTTGATAGGTGTCTGGCGCCATCGACTGCTGGTGGTGAGGGATGCGATAACATGACCATGATCGTGGTGCAGTTCAAGAAGCCCAAACAGTCGACTGAGTCTGAGGGTGGGCAATCCTCGTCGACTGAAGAAGCAGCTGGCAGCGATTCAAACGGAAGTGATTCAAAGGCAGATGAAAGTGAATCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

340

Amino Acids

37.03

Weight (kDa)

4.92

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 1 - 76 2.5e-09 Protein phosphatase 2C
PP2C PF00481 127 - 285 2.2e-59 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 844
AccB7I CCANNNNNTGG 2 cut(s) 392, 763
AccI GTMKAC 2 cut(s) 920, 950
AccIII TCCGGA 1 cut(s) 673
AciI CCGC 1 cut(s) 164
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 3 cut(s) 620, 635, 710
AcuI CTGAAG 1 cut(s) 975
AcyI GRCGYC 1 cut(s) 845
AfaI GTAC 3 cut(s) 16, 144, 777
AfiI CCNNNNNNNGG 3 cut(s) 392, 763, 834
AgsI TTSAA 5 cut(s) 592, 625, 904, 978, 993
AhdI GACNNNNNGTC 1 cut(s) 748
AloI GAACNNNNNNTCC 2 cut(s) 171, 203
AluBI AGCT 8 cut(s) 197, 338, 526, 551, 605, 694, 805, 965
AluI AGCT 8 cut(s) 197, 338, 526, 551, 605, 694, 805, 965
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 2 cut(s) 165, 312
Aor13HI TCCGGA 1 cut(s) 673
AoxI GGCC 2 cut(s) 88, 362
ApeKI GCWGC 3 cut(s) 38, 962, 969
ApoI RAATTY 3 cut(s) 620, 635, 710
Asp700I GAANNNNTTC 1 cut(s) 988
AspLEI GCGC 1 cut(s) 847
AspS9I GGNCC 3 cut(s) 362, 383, 505
AsuHPI GGTGA 4 cut(s) 125, 464, 623, 875
AsuII TTCGAA 1 cut(s) 421
AsuNHI GCTAGC 1 cut(s) 721
AvaII GGWCC 2 cut(s) 383, 505
BalI TGGCCA 1 cut(s) 90
BanI GGYRCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 829
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 3 cut(s) 50, 974, 981
BccI CCATC 4 cut(s) 3, 80, 725, 856
BclI TGATCA 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 165, 312
BfaI CTAG 2 cut(s) 722, 753
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 848
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 4 cut(s) 39, 165, 963, 970
BlsI GCNGC 4 cut(s) 40, 166, 964, 971
BmcAI AGTACT 1 cut(s) 144
Bme18I GGWCC 2 cut(s) 383, 505
BmeRI GACNNNNNGTC 1 cut(s) 748
BmgT120I GGNCC 3 cut(s) 362, 383, 505
BmiI GGNNCC 3 cut(s) 363, 506, 846
BmsI GCATC 6 cut(s) 19, 46, 68, 210, 744, 860
BmtI GCTAGC 1 cut(s) 725
BpmI CTGGAG 1 cut(s) 189
Bpu14I TTCGAA 1 cut(s) 421
BpuEI CTTGAG 3 cut(s) 131, 542, 548
BsaAI YACGTR 1 cut(s) 779
BsaHI GRCGYC 1 cut(s) 845
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 91, 311
BsaWI WCCGGW 1 cut(s) 673
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 3 cut(s) 392, 763, 834
Bse1I ACTGG 2 cut(s) 286, 385
BseAI TCCGGA 1 cut(s) 673
BseDI CCNNGG 2 cut(s) 91, 311
BseGI GGATG 7 cut(s) 14, 34, 59, 61, 217, 561, 875
BseLI CCNNNNNNNGG 3 cut(s) 392, 763, 834
BseMII CTCAG 2 cut(s) 915, 921
BseNI ACTGG 2 cut(s) 286, 385
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 3 cut(s) 50, 974, 981
BsgI GTGCAG 1 cut(s) 917
BshFI GGCC 2 cut(s) 90, 364
BshNI GGYRCC 1 cut(s) 844
BsiHKAI GWGCWC 1 cut(s) 401
BsiSI CCGG 1 cut(s) 674
BslFI GGGAC 2 cut(s) 518, 755
BslI CCNNNNNNNGG 3 cut(s) 392, 763, 834
BsmAI GTCTC 2 cut(s) 165, 312
BsmFI GGGAC 2 cut(s) 518, 755
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 2 cut(s) 90, 364
Bso31I GGTCTC 1 cut(s) 312
Bsp119I TTCGAA 1 cut(s) 421
Bsp1286I GDGCHC 2 cut(s) 401, 829
Bsp13I TCCGGA 1 cut(s) 673
Bsp143I GATC 4 cut(s) 49, 331, 517, 888
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 1 cut(s) 164
BspANI GGCC 2 cut(s) 90, 364
BspCNI CTCAG 2 cut(s) 916, 922
BspEI TCCGGA 1 cut(s) 673
BspLI GGNNCC 3 cut(s) 363, 506, 846
BspOI GCTAGC 1 cut(s) 725
BspT104I TTCGAA 1 cut(s) 421
BspT107I GGYRCC 1 cut(s) 844
BspTNI GGTCTC 1 cut(s) 312
BsrI ACTGG 2 cut(s) 286, 385
BssECI CCNNGG 2 cut(s) 91, 311
BssMI GATC 4 cut(s) 49, 331, 517, 888
BssNI GRCGYC 1 cut(s) 845
BssT1I CCWWGG 2 cut(s) 91, 311
Bst4CI ACNGT 3 cut(s) 688, 746, 918
BstACI GRCGYC 1 cut(s) 845
BstBAI YACGTR 1 cut(s) 779
BstBI TTCGAA 1 cut(s) 421
BstC8I GCNNGC 5 cut(s) 723, 727, 758, 803, 967
BstDEI CTNAG 3 cut(s) 111, 924, 930
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 7 cut(s) 14, 34, 59, 61, 217, 561, 875
BstH2I RGCGCY 1 cut(s) 848
BstHHI GCGC 1 cut(s) 847
BstKTI GATC 4 cut(s) 52, 334, 520, 891
BstMAI GTCTC 2 cut(s) 165, 312
BstMBI GATC 4 cut(s) 49, 331, 517, 888
BstMWI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
BstNSI RCATGY 2 cut(s) 672, 729
BstSFI CTRYAG 1 cut(s) 606
BstV1I GCAGC 3 cut(s) 50, 974, 981
BstXI CCANNNNNNTGG 1 cut(s) 892
BsuRI GGCC 2 cut(s) 90, 364
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 744
BtsCI GGATG 7 cut(s) 14, 34, 59, 61, 217, 561, 875
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 399, 684
Cac8I GCNNGC 5 cut(s) 723, 727, 758, 803, 967
CfoI GCGC 1 cut(s) 847
Cfr13I GGNCC 3 cut(s) 362, 383, 505
Csp6I GTAC 3 cut(s) 15, 143, 776
CviAII CATG 9 cut(s) 35, 92, 286, 566, 616, 669, 726, 880, 886
CviQI GTAC 3 cut(s) 15, 143, 776
DdeI CTNAG 3 cut(s) 111, 924, 930
DinI GGCGCC 1 cut(s) 846
DpnI GATC 4 cut(s) 51, 333, 519, 890
DpnII GATC 4 cut(s) 49, 331, 517, 888
DraI TTTAAA 1 cut(s) 546
DriI GACNNNNNGTC 1 cut(s) 748
EaeI YGGCCR 1 cut(s) 88
Eam1105I GACNNNNNGTC 1 cut(s) 748
Eco130I CCWWGG 2 cut(s) 91, 311
Eco24I GRGCYC 1 cut(s) 829
Eco31I GGTCTC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 383, 505
Eco57I CTGAAG 1 cut(s) 975
EcoO109I RGGNCCY 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 620
EcoRV GATATC 1 cut(s) 471
EcoT14I CCWWGG 2 cut(s) 91, 311
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 829
EgeI GGCGCC 1 cut(s) 846
EheI GGCGCC 1 cut(s) 846
ErhI CCWWGG 2 cut(s) 91, 311
FaeI CATG 9 cut(s) 38, 95, 289, 569, 619, 672, 729, 883, 889
FaqI GGGAC 2 cut(s) 518, 755
FatI CATG 9 cut(s) 34, 91, 285, 565, 615, 668, 725, 879, 885
FauNDI CATATG 1 cut(s) 160
FbaI TGATCA 1 cut(s) 331
FblI GTMKAC 2 cut(s) 920, 950
Fnu4HI GCNGC 4 cut(s) 39, 165, 963, 970
FokI GGATG 7 cut(s) 21, 41, 46, 68, 224, 548, 882
FriOI GRGCYC 1 cut(s) 829
Fsp4HI GCNGC 4 cut(s) 39, 165, 963, 970
FspBI CTAG 2 cut(s) 722, 753
GlaI GCGC 1 cut(s) 846
GluI GCNGC 4 cut(s) 39, 165, 963, 970
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 848
HaeIII GGCC 2 cut(s) 90, 364
HapII CCGG 1 cut(s) 674
HhaI GCGC 1 cut(s) 847
Hin1I GRCGYC 1 cut(s) 845
Hin1II CATG 9 cut(s) 38, 95, 289, 569, 619, 672, 729, 883, 889
Hin6I GCGC 1 cut(s) 845
HinP1I GCGC 1 cut(s) 845
HincII GTYRAC 2 cut(s) 921, 951
HindII GTYRAC 2 cut(s) 921, 951
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 6 cut(s) 455, 576, 926, 974, 989, 1010
HpaII CCGG 1 cut(s) 674
HphI GGTGA 4 cut(s) 125, 464, 623, 875
Hpy166II GTNNAC 5 cut(s) 227, 279, 782, 921, 951
Hpy188I TCNGA 5 cut(s) 192, 206, 373, 796, 931
Hpy188III TCNNGA 6 cut(s) 47, 53, 148, 521, 674, 904
Hpy8I GTNNAC 5 cut(s) 227, 279, 782, 921, 951
Hpy99I CGWCG 1 cut(s) 952
HpyAV CCTTC 3 cut(s) 287, 472, 526
HpyCH4III ACNGT 3 cut(s) 688, 746, 918
HpyCH4IV ACGT 1 cut(s) 778
HpyCH4V TGCA 9 cut(s) 4, 38, 59, 122, 413, 569, 599, 668, 898
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
HpyF3I CTNAG 3 cut(s) 111, 924, 930
HpySE526I ACGT 1 cut(s) 778
Hsp92I GRCGYC 1 cut(s) 845
Hsp92II CATG 9 cut(s) 38, 95, 289, 569, 619, 672, 729, 883, 889
HspAI GCGC 1 cut(s) 845
KasI GGCGCC 1 cut(s) 844
Kpn2I TCCGGA 1 cut(s) 673
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 4 cut(s) 49, 331, 517, 888
LmnI GCTCC 1 cut(s) 396
Lsp1109I GCAGC 3 cut(s) 50, 974, 981
LweI GCATC 6 cut(s) 19, 46, 68, 210, 744, 860
MaeI CTAG 2 cut(s) 722, 753
MaeII ACGT 1 cut(s) 778
MaeIII GTNAC 1 cut(s) 611
MalI GATC 4 cut(s) 51, 333, 519, 890
MboI GATC 4 cut(s) 49, 331, 517, 888
MboII GAAGA 3 cut(s) 122, 410, 968
MhlI GDGCHC 2 cut(s) 401, 829
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 5 cut(s) 300, 414, 620, 635, 710
MluNI TGGCCA 1 cut(s) 90
Mly113I GGCGCC 1 cut(s) 845
MlyI GAGTC 2 cut(s) 585, 935
MmeI TCCRAC 2 cut(s) 218, 410
Mox20I TGGCCA 1 cut(s) 90
Mph1103I ATGCAT 1 cut(s) 61
MroI TCCGGA 1 cut(s) 673
MroXI GAANNNNTTC 1 cut(s) 988
MscI TGGCCA 1 cut(s) 90
MseI TTAA 2 cut(s) 299, 545
MslI CAYNNNNRTG 2 cut(s) 884, 890
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 965
MspI CCGG 1 cut(s) 674
Mva1269I GAATGC 1 cut(s) 451
MwoI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
NarI GGCGCC 1 cut(s) 845
NcoI CCATGG 1 cut(s) 91
NdeI CATATG 1 cut(s) 160
NdeII GATC 4 cut(s) 49, 331, 517, 888
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 9 cut(s) 38, 95, 289, 569, 619, 672, 729, 883, 889
NlaIV GGNNCC 3 cut(s) 363, 506, 846
NmuCI GTSAC 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 2 cut(s) 672, 729
NspV TTCGAA 1 cut(s) 421
PaeI GCATGC 1 cut(s) 729
PctI GAATGC 1 cut(s) 451
PdmI GAANNNNTTC 1 cut(s) 988
PfeI GAWTC 4 cut(s) 455, 974, 989, 1010
PflFI GACNNNGTC 1 cut(s) 925
PflMI CCANNNNNTGG 2 cut(s) 392, 763
PkrI GCNGC 4 cut(s) 40, 166, 964, 971
PleI GAGTC 2 cut(s) 584, 934
PluTI GGCGCC 1 cut(s) 848
PpsI GAGTC 2 cut(s) 584, 934
Ppu21I YACGTR 1 cut(s) 779
PspN4I GGNNCC 3 cut(s) 363, 506, 846
PspPI GGNCC 3 cut(s) 362, 383, 505
PsyI GACNNNGTC 1 cut(s) 925
PvuII CAGCTG 1 cut(s) 965
RsaI GTAC 3 cut(s) 16, 144, 777
RsaNI GTAC 3 cut(s) 15, 143, 776
RseI CAYNNNNRTG 2 cut(s) 884, 890
SalI GTCGAC 2 cut(s) 919, 949
SaqAI TTAA 2 cut(s) 299, 545
SatI GCNGC 4 cut(s) 39, 165, 963, 970
Sau3AI GATC 4 cut(s) 49, 331, 517, 888
Sau96I GGNCC 3 cut(s) 362, 383, 505
ScaI AGTACT 1 cut(s) 144
SchI GAGTC 2 cut(s) 585, 935
SduI GDGCHC 2 cut(s) 401, 829
SfaNI GCATC 6 cut(s) 19, 46, 68, 210, 744, 860
SfcI CTRYAG 1 cut(s) 606
SfoI GGCGCC 1 cut(s) 846
SfuI TTCGAA 1 cut(s) 421
SinI GGWCC 2 cut(s) 383, 505
SmiMI CAYNNNNRTG 2 cut(s) 884, 890
SmlI CTYRAG 3 cut(s) 146, 521, 527
SmoI CTYRAG 3 cut(s) 146, 521, 527
SphI GCATGC 1 cut(s) 729
Sse9I AATT 5 cut(s) 300, 414, 620, 635, 710
SsiI CCGC 1 cut(s) 164
SspDI GGCGCC 1 cut(s) 844
SspMI CTAG 2 cut(s) 722, 753
StyI CCWWGG 2 cut(s) 91, 311
TaaI ACNGT 3 cut(s) 688, 746, 918
TaiI ACGT 1 cut(s) 781
TaqI TCGA 5 cut(s) 421, 851, 920, 950, 1013
TasI AATT 5 cut(s) 300, 414, 620, 635, 710
TatI WGTACW 1 cut(s) 142
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 4 cut(s) 455, 974, 989, 1010
Tru1I TTAA 2 cut(s) 299, 545
Tru9I TTAA 2 cut(s) 299, 545
TscAI CASTG 2 cut(s) 406, 691
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 3 cut(s) 38, 962, 969
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 6 cut(s) 168, 228, 550, 663, 723, 1017
TspGWI ACGGA 1 cut(s) 996
TspRI CASTG 2 cut(s) 406, 691
Tth111I GACNNNGTC 1 cut(s) 925
Van91I CCANNNNNTGG 2 cut(s) 392, 763
VpaK11BI GGWCC 2 cut(s) 383, 505
XapI RAATTY 3 cut(s) 620, 635, 710
XceI RCATGY 2 cut(s) 672, 729
XmiI GTMKAC 2 cut(s) 920, 950
XmnI GAANNNNTTC 1 cut(s) 988
XspI CTAG 2 cut(s) 722, 753
ZrmI AGTACT 1 cut(s) 144
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.