FvH4_2g41201

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000743
Physical Location & Seq
Reverse (-)
29190436 .. 29191962
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g41201.t1

Sequence Viewer

Length: 1527 bp
ATGGCCCTTGTGACCTGCAACTCCATTTCTTGCCAACAGTCGGCTTCGCTGGACCTTCAAAGATGGAATAATGTTATAAAGACTCAAGTGCTCGAGGGGAACGCAGGGCACGCAATTCGGTCGTATGTAAACATGCAAAGACATGGTCTCTTTGCTGATAACTATACATTTCCGGTTTTGCTGAAAGCAGCAGGTAATGCATCTTACTTGGGTGTAGGGTTAGCACTTCATGGACAGGCAGTGAAGACTGGGTTTTGTGATCATCTTTTCGTCCAAACTGCTCTGCTGAACATGTATTCTTCTCTTGGAATCGTAGTGGATGCCCGCAAAGCGTTTGATAAAATGCCTGTGAAAGATGTGGTAGCCTGGAACTCCATGTTATATGTGTATGCTTCTAGTGGGCAGATGGGGAATGCAGTGCAAGTCTTTGATACAATGCCATCGCATGACCTTTTGTCTTTCAATATTATGATCTCTGGGTTCGCAGGGGCTGGAAGCAAAACATGTGCCAGAAGCATTTTTGATAGAATTCCTGACAAGGACATTGTGTCTTGGAACTCGATGATATTGGCGTGCATGAATGCAGGAGATATGGCTGAAGCGCATAAGTTGTTCGAGTCGATGCCTGAGAAAAATGTCATAACTTGGAACACAATGATAATGGGTTATCTGAACAATCAGTTTTACTCCAAGGCAATCGAATTCTTGGACAAAATGAAGGTGGGAAATATCGAACCGGATCATCTGAGCGTGACTGGTGCTTTAGCTGCCTGTGCTCGTTTGGGGTTGCTTGAAACTGGCATAAAGATACACACATATGCCAAACTGTCGGGACAGGCTTCAAGCCCCCATGTAGTAACAGCACTTATAGATATGTATGCCAAGTGCGGAAACATCCACTACTCTTTAGAAGTGTTCTACAAGTTCAAACACAAAAACGTCTACTGTTGGAATGCCATCATTTCGGGACTCGCTCTTCACGGTTTTGGCAACGCAGCTCTAAAAATCTTCAACCAAATGAGAGCGGATTTTCAAGTAAAACCAGATGATATCACCTTCATTGGGGTCTTAAGTGCGTGCAGCCATGGAGGACTGGTGGAGGAAGGATGTGAATTGTTTGATTCAATGGAAAAGCAATTTGGGATAACTCCAAAGGCAGAGCATTATGGGTGCATGGTCGACCTTCTGAGCAGAGCGGGCCTTTTGGACAGTGCATTGCTACTCATAGAGACGATGCCGTTTGAAGCAGGAGAGTCCATATTGGGTGCATTGCTGAGTGCTTGTGTGATTCATTGGGACCTTGAAATTGGGGAGAAAGCAATGAAGCTAATGGCTGCTGGGAGCGATGGGGAGTATATGATGTTAGCAAACTTGTACGCGTCTTGCGGTAAATGGGAAGAAGCACAGAGATGGAGAGATATGATGAACGAGTCGGGGATTGAAAAGACTGCAGGTTGCAGCTTGGTTGAGGTTAATGGAAGGTTTCACAAGTTTATGGCCGGAGATCAGAATGGTACTGTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

509

Amino Acids

55.69

Weight (kDa)

5.97

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 118 - 162 8.7e-07 PPR repeat family
PPR_2 PF13041 180 - 213 1.2e-07 PPR repeat family
PPR PF01535 183 - 213 1.1e-06 PPR repeat
PPR_2 PF13041 211 - 260 1.3e-09 PPR repeat family
PPR_2 PF13041 312 - 361 2.9e-07 PPR repeat family
E_motif PF20431 431 - 490 1.8e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
Acc36I ACCTGC 3 cut(s) 23, 182, 1442
AccBSI CCGCTC 2 cut(s) 1025, 1196
AccI GTMKAC 2 cut(s) 942, 1179
AccII CGCG 1 cut(s) 1379
AciI CCGC 5 cut(s) 325, 888, 1025, 1196, 1386
AclWI GGATC 1 cut(s) 747
AcoI YGGCCR 1 cut(s) 1497
AcsI RAATTY 2 cut(s) 528, 701
AcuI CTGAAG 1 cut(s) 618
AfaI GTAC 2 cut(s) 1376, 1516
AfiI CCNNNNNNNGG 2 cut(s) 40, 1062
AflII CTTAAG 1 cut(s) 1069
AflIII ACRYGT 3 cut(s) 291, 503, 1377
AhdI GACNNNNNGTC 2 cut(s) 454, 547
AjnI CCWGG 1 cut(s) 365
AjuI GAANNNNNNNTTGG 2 cut(s) 1122, 1154
AluBI AGCT 4 cut(s) 767, 998, 1327, 1461
AluI AGCT 4 cut(s) 767, 998, 1327, 1461
Alw21I GWGCWC 2 cut(s) 93, 778
Alw26I GTCTC 2 cut(s) 152, 1223
AlwI GGATC 1 cut(s) 747
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 3 cut(s) 3, 1198, 1497
ApeKI GCWGC 6 cut(s) 188, 767, 995, 1080, 1334, 1458
ApoI RAATTY 2 cut(s) 528, 701
ArsI GACNNNNNNTTYG 2 cut(s) 130, 162
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 4 cut(s) 4, 52, 1198, 1297
AsuHPI GGTGA 1 cut(s) 1045
AvaI CYCGRG 1 cut(s) 92
AvaII GGWCC 2 cut(s) 52, 1297
BaeGI GKGCMC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 93, 778
BbvI GCAGC 6 cut(s) 200, 754, 1007, 1092, 1321, 1470
BccI CCATC 6 cut(s) 57, 400, 448, 965, 1340, 1404
BceAI ACGGC 1 cut(s) 1222
BcgI CGANNNNNNTGC 4 cut(s) 98, 102, 132, 136
BciT130I CCWGG 1 cut(s) 367
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 2 cut(s) 152, 1223
BfaI CTAG 1 cut(s) 396
BfmI CTRYAG 1 cut(s) 1449
BfrI CTTAAG 1 cut(s) 1069
BfuAI ACCTGC 3 cut(s) 23, 182, 1442
BisI GCNGC 6 cut(s) 189, 768, 996, 1081, 1335, 1459
BlsI GCNGC 6 cut(s) 190, 769, 997, 1082, 1336, 1460
Bme1390I CCNGG 1 cut(s) 367
Bme18I GGWCC 2 cut(s) 52, 1297
BmeRI GACNNNNNGTC 2 cut(s) 454, 547
BmeT110I CYCGRG 1 cut(s) 92
BmgT120I GGNCC 4 cut(s) 4, 52, 1198, 1297
BmiI GGNNCC 1 cut(s) 1298
BmrFI CCNGG 1 cut(s) 367
BmrI ACTGGG 1 cut(s) 258
BmsI GCATC 4 cut(s) 209, 310, 612, 1224
BmuI ACTGGG 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 690, 1084
BsaWI WCCGGW 2 cut(s) 172, 736
BsaXI ACNNNNNCTCC 2 cut(s) 1080, 1110
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 1062
Bse1I ACTGG 4 cut(s) 253, 760, 802, 1098
Bse3DI GCAATG 3 cut(s) 1214, 1268, 1326
BseBI CCWGG 1 cut(s) 367
BseDI CCNNGG 2 cut(s) 690, 1084
BseGI GGATG 3 cut(s) 325, 894, 1112
BseLI CCNNNNNNNGG 2 cut(s) 40, 1062
BseMI GCAATG 3 cut(s) 1214, 1268, 1326
BseMII CTCAG 4 cut(s) 618, 737, 1178, 1265
BseNI ACTGG 4 cut(s) 253, 760, 802, 1098
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 6 cut(s) 200, 754, 1007, 1092, 1321, 1470
BseYI CCCAGC 1 cut(s) 1337
BsgI GTGCAG 1 cut(s) 1099
Bsh1236I CGCG 1 cut(s) 1379
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 3 cut(s) 5, 1200, 1499
BsiEI CGRYCG 1 cut(s) 122
BsiHKAI GWGCWC 2 cut(s) 93, 778
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 3 cut(s) 173, 737, 1500
BslFI GGGAC 3 cut(s) 846, 981, 1310
BslI CCNNNNNNNGG 2 cut(s) 40, 1062
BsmAI GTCTC 2 cut(s) 152, 1223
BsmBI CGTCTC 1 cut(s) 1223
BsmFI GGGAC 3 cut(s) 846, 981, 1310
BsmI GAATGC 3 cut(s) 418, 586, 958
BsnI GGCC 3 cut(s) 5, 1200, 1499
Bso31I GGTCTC 1 cut(s) 152
BsoBI CYCGRG 1 cut(s) 92
Bsp1286I GDGCHC 3 cut(s) 93, 111, 778
Bsp143I GATC 4 cut(s) 259, 471, 739, 1504
Bsp19I CCATGG 1 cut(s) 1084
BspACI CCGC 5 cut(s) 325, 888, 1025, 1196, 1386
BspANI GGCC 3 cut(s) 5, 1200, 1499
BspCNI CTCAG 4 cut(s) 619, 738, 1179, 1266
BspFNI CGCG 1 cut(s) 1379
BspLI GGNNCC 1 cut(s) 1298
BspMAI CTGCAG 1 cut(s) 1453
BspMI ACCTGC 3 cut(s) 23, 182, 1442
BspPI GGATC 1 cut(s) 747
BspQI GCTCTTC 1 cut(s) 981
BspTI CTTAAG 1 cut(s) 1069
BspTNI GGTCTC 1 cut(s) 152
BsrBI CCGCTC 2 cut(s) 1025, 1196
BsrDI GCAATG 3 cut(s) 1214, 1268, 1326
BsrI ACTGG 4 cut(s) 253, 760, 802, 1098
BssECI CCNNGG 2 cut(s) 690, 1084
BssMI GATC 4 cut(s) 259, 471, 739, 1504
BssT1I CCWWGG 2 cut(s) 690, 1084
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 6 cut(s) 39, 828, 947, 983, 1211, 1519
Bst6I CTCTTC 1 cut(s) 981
BstAFI CTTAAG 1 cut(s) 1069
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 5 cut(s) 111, 325, 574, 1078, 1198
BstDEI CTNAG 4 cut(s) 627, 746, 1187, 1274
BstDSI CCRYGG 1 cut(s) 1084
BstF5I GGATG 3 cut(s) 325, 894, 1112
BstFNI CGCG 1 cut(s) 1379
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 4 cut(s) 262, 474, 742, 1507
BstMAI GTCTC 2 cut(s) 152, 1223
BstMBI GATC 4 cut(s) 259, 471, 739, 1504
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 6 cut(s) 110, 197, 329, 767, 773, 1197
BstNI CCWGG 1 cut(s) 367
BstNSI RCATGY 3 cut(s) 136, 295, 507
BstSCI CCNGG 1 cut(s) 365
BstSFI CTRYAG 1 cut(s) 1449
BstSLI GKGCMC 1 cut(s) 111
BstUI CGCG 1 cut(s) 1379
BstV1I GCAGC 6 cut(s) 200, 754, 1007, 1092, 1321, 1470
BstV2I GAAGAC 1 cut(s) 251
BsuRI GGCC 3 cut(s) 5, 1200, 1499
BtgI CCRYGG 1 cut(s) 1084
BtgZI GCGATG 2 cut(s) 426, 1359
BtsCI GGATG 3 cut(s) 325, 894, 1112
BtsI GCAGTG 2 cut(s) 246, 423
BtsIMutI CAGTG 3 cut(s) 246, 423, 1216
BveI ACCTGC 3 cut(s) 23, 182, 1442
Cac8I GCNNGC 5 cut(s) 111, 325, 574, 1078, 1198
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 1 cut(s) 604
Cfr13I GGNCC 4 cut(s) 4, 52, 1198, 1297
CseI GACGC 1 cut(s) 1368
Csp6I GTAC 2 cut(s) 1375, 1515
CviQI GTAC 2 cut(s) 1375, 1515
DdeI CTNAG 4 cut(s) 627, 746, 1187, 1274
DpnI GATC 4 cut(s) 261, 473, 741, 1506
DpnII GATC 4 cut(s) 259, 471, 739, 1504
DriI GACNNNNNGTC 2 cut(s) 454, 547
EaeI YGGCCR 1 cut(s) 1497
Eam1104I CTCTTC 1 cut(s) 981
Eam1105I GACNNNNNGTC 2 cut(s) 454, 547
EarI CTCTTC 1 cut(s) 981
Eco130I CCWWGG 2 cut(s) 690, 1084
Eco31I GGTCTC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 1051
Eco47I GGWCC 2 cut(s) 52, 1297
Eco57I CTGAAG 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 1297
EcoRI GAATTC 2 cut(s) 528, 701
EcoRII CCWGG 1 cut(s) 365
EcoRV GATATC 1 cut(s) 1051
EcoT14I CCWWGG 2 cut(s) 690, 1084
EcoT22I ATGCAT 1 cut(s) 202
ErhI CCWWGG 2 cut(s) 690, 1084
Esp3I CGTCTC 1 cut(s) 1223
FaqI GGGAC 3 cut(s) 846, 981, 1310
FauI CCCGC 2 cut(s) 332, 1189
FauNDI CATATG 1 cut(s) 817
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 2 cut(s) 942, 1179
Fnu4HI GCNGC 6 cut(s) 189, 768, 996, 1081, 1335, 1459
FokI GGATG 3 cut(s) 332, 881, 1119
Fsp4HI GCNGC 6 cut(s) 189, 768, 996, 1081, 1335, 1459
FspBI CTAG 1 cut(s) 396
GlaI GCGC 1 cut(s) 603
GluI GCNGC 6 cut(s) 189, 768, 996, 1081, 1335, 1459
GsaI CCCAGC 1 cut(s) 1341
HaeIII GGCC 3 cut(s) 5, 1200, 1499
HapII CCGG 3 cut(s) 173, 737, 1500
HgaI GACGC 1 cut(s) 1368
HhaI GCGC 1 cut(s) 604
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HincII GTYRAC 1 cut(s) 1180
HindII GTYRAC 1 cut(s) 1180
HinfI GANTC 8 cut(s) 82, 309, 617, 969, 1121, 1253, 1288, 1430
HpaII CCGG 3 cut(s) 173, 737, 1500
HphI GGTGA 1 cut(s) 1045
Hpy166II GTNNAC 3 cut(s) 130, 943, 1180
Hpy188I TCNGA 4 cut(s) 672, 747, 1188, 1509
Hpy188III TCNNGA 3 cut(s) 533, 831, 966
Hpy8I GTNNAC 3 cut(s) 130, 943, 1180
HpyAV CCTTC 6 cut(s) 65, 712, 1066, 1097, 1193, 1473
HpyCH4III ACNGT 6 cut(s) 39, 828, 947, 983, 1211, 1519
HpyCH4IV ACGT 1 cut(s) 939
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 197, 329, 767, 773, 1197
HpyF3I CTNAG 4 cut(s) 627, 746, 1187, 1274
HpySE526I ACGT 1 cut(s) 939
HspAI GCGC 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 4 cut(s) 259, 471, 739, 1504
LguI GCTCTTC 1 cut(s) 981
LmnI GCTCC 1 cut(s) 1341
Lsp1109I GCAGC 6 cut(s) 200, 754, 1007, 1092, 1321, 1470
LweI GCATC 4 cut(s) 209, 310, 612, 1224
MaeI CTAG 1 cut(s) 396
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 3 cut(s) 10, 751, 856
MalI GATC 4 cut(s) 261, 473, 741, 1506
MbiI CCGCTC 2 cut(s) 1025, 1196
MboI GATC 4 cut(s) 259, 471, 739, 1504
MboII GAAGA 5 cut(s) 256, 291, 968, 1000, 1409
MhlI GDGCHC 3 cut(s) 93, 111, 778
MluCI AATT 6 cut(s) 114, 528, 701, 1112, 1136, 1305
MluI ACGCGT 1 cut(s) 1377
MlyI GAGTC 5 cut(s) 76, 626, 963, 1262, 1439
MmeI TCCRAC 1 cut(s) 929
MnlI CCTC 4 cut(s) 88, 1082, 1093, 1462
Mph1103I ATGCAT 1 cut(s) 202
MseI TTAA 2 cut(s) 1070, 1473
MslI CAYNNNNRTG 2 cut(s) 816, 1408
MspCI CTTAAG 1 cut(s) 1069
MspI CCGG 3 cut(s) 173, 737, 1500
MspR9I CCNGG 1 cut(s) 367
Mva1269I GAATGC 3 cut(s) 418, 586, 958
MvaI CCWGG 1 cut(s) 367
MvnI CGCG 1 cut(s) 1379
MwoI GCNNNNNNNGC 6 cut(s) 110, 197, 329, 767, 773, 1197
NcoI CCATGG 1 cut(s) 1084
NdeI CATATG 1 cut(s) 817
NdeII GATC 4 cut(s) 259, 471, 739, 1504
NlaIV GGNNCC 1 cut(s) 1298
NmuCI GTSAC 2 cut(s) 10, 751
NsiI ATGCAT 1 cut(s) 202
NspI RCATGY 3 cut(s) 136, 295, 507
PaeR7I CTCGAG 1 cut(s) 92
PciI ACATGT 2 cut(s) 291, 503
PciSI GCTCTTC 1 cut(s) 981
PctI GAATGC 3 cut(s) 418, 586, 958
PfeI GAWTC 3 cut(s) 309, 1121, 1288
PflFI GACNNNGTC 1 cut(s) 144
PkrI GCNGC 6 cut(s) 190, 769, 997, 1082, 1336, 1460
PleI GAGTC 5 cut(s) 76, 625, 963, 1261, 1438
PpsI GAGTC 5 cut(s) 76, 625, 963, 1261, 1438
PpuMI RGGWCCY 1 cut(s) 1297
PscI ACATGT 2 cut(s) 291, 503
PsiI TTATAA 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 1297
Psp6I CCWGG 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 1337
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 1 cut(s) 1298
PspPI GGNCC 4 cut(s) 4, 52, 1198, 1297
PspPPI RGGWCCY 1 cut(s) 1297
PspXI VCTCGAGB 1 cut(s) 92
PstI CTGCAG 1 cut(s) 1453
PstNI CAGNNNCTG 1 cut(s) 491
PsyI GACNNNGTC 1 cut(s) 144
RsaI GTAC 2 cut(s) 1376, 1516
RsaNI GTAC 2 cut(s) 1375, 1515
RseI CAYNNNNRTG 2 cut(s) 816, 1408
SalI GTCGAC 1 cut(s) 1178
SapI GCTCTTC 1 cut(s) 981
SaqAI TTAA 2 cut(s) 1070, 1473
SatI GCNGC 6 cut(s) 189, 768, 996, 1081, 1335, 1459
Sau3AI GATC 4 cut(s) 259, 471, 739, 1504
Sau96I GGNCC 4 cut(s) 4, 52, 1198, 1297
SchI GAGTC 5 cut(s) 76, 626, 963, 1262, 1439
ScrFI CCNGG 1 cut(s) 367
SduI GDGCHC 3 cut(s) 93, 111, 778
SfaNI GCATC 4 cut(s) 209, 310, 612, 1224
SfcI CTRYAG 1 cut(s) 1449
Sfr274I CTCGAG 1 cut(s) 92
SinI GGWCC 2 cut(s) 52, 1297
SlaI CTCGAG 1 cut(s) 92
SmiMI CAYNNNNRTG 2 cut(s) 816, 1408
SmlI CTYRAG 3 cut(s) 84, 92, 1069
SmoI CTYRAG 3 cut(s) 84, 92, 1069
Sse9I AATT 6 cut(s) 114, 528, 701, 1112, 1136, 1305
SsiI CCGC 5 cut(s) 325, 888, 1025, 1196, 1386
SspI AATATT 1 cut(s) 466
SspMI CTAG 1 cut(s) 396
StyD4I CCNGG 1 cut(s) 365
StyI CCWWGG 2 cut(s) 690, 1084
TaaI ACNGT 6 cut(s) 39, 828, 947, 983, 1211, 1519
TaiI ACGT 1 cut(s) 942
TaqI TCGA 7 cut(s) 93, 560, 615, 620, 699, 732, 1179
TaqII GACCGA 1 cut(s) 108
TasI AATT 6 cut(s) 114, 528, 701, 1112, 1136, 1305
TfiI GAWTC 3 cut(s) 309, 1121, 1288
Tru1I TTAA 2 cut(s) 1070, 1473
Tru9I TTAA 2 cut(s) 1070, 1473
TscAI CASTG 3 cut(s) 246, 423, 1216
TseFI GTSAC 2 cut(s) 10, 751
TseI GCWGC 6 cut(s) 188, 767, 995, 1080, 1334, 1458
Tsp45I GTSAC 2 cut(s) 10, 751
TspDTI ATGAA 7 cut(s) 218, 593, 731, 1048, 1280, 1337, 1439
TspRI CASTG 3 cut(s) 246, 423, 1216
Tth111I GACNNNGTC 1 cut(s) 144
Vha464I CTTAAG 1 cut(s) 1069
VpaK11BI GGWCC 2 cut(s) 52, 1297
XapI RAATTY 2 cut(s) 528, 701
XceI RCATGY 3 cut(s) 136, 295, 507
XhoI CTCGAG 1 cut(s) 92
XmiI GTMKAC 2 cut(s) 942, 1179
XspI CTAG 1 cut(s) 396
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.