Rw2G029230

protein phosphatase 2C 60

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000743
Physical Location & Seq
Forward (+)
47272190 .. 47280083
7894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029230.1

Sequence Viewer

Length: 1104 bp
ATGCTGGAGATGGGAATATATCTGAGCGCTCCGAAAACCGATAAGGTGTCAGAAGATGGTGAGAATCATAGGCTGCGATTTGGGGCTTCCTCCATGCAGGGGTGGCGTTCAACCATGGAAGATGCTCATGCTGCTTTTCCAGATCTCGACAATTCAACATCTTTCTTTGGTGTTTATGATGGTCATGGAGGTAAAGCGGTGGCAAATTTCTGTGCCAAGTATCTCCACTTACAAGTGCTCAACCAGGAAGCATATCTAGCTGGTGATTTAGGCACTTCCCTGCAGAAAGCTTTTCTCAGGATGGATGAGATGATGCGTGGACAGAGAGGATGGAGAGAACTAGCTGTATTGGGAGATAAGATAGACAAAGTTTCAGGCCTTATTGAAGGGTTCATCTTTTCTCCTAAGAGTGTTGAAGCTAAGAGAAGTGCATTCAATGATCAAATTGATCAGTGGTCTTCTGAGGAGGGGCCTCACTCTGATTTTGATGGACCAAACTCTGGAAGCACAGCTTGTGTTGCTATCATCCGAAACAATCAACTTGTTGTTGCCAATGCTGGTGATTCGCGTTGTGTAATATCGAGGAAGGGTCAGGCATATCCTTTGTCTAAAGATCATAAGCCTGACATTGAGAATGAGAAGGATAGAATTCTGAAGGCCGGTGGATTTATCCAAGTTGGACGGGTCAATGGAAGTTTGAACTTGGCAAGAGCGATTGGAGATGTGGAATTCAAGCAAAACAAACAATTGCCTGTTGAAAAGCAGATTGTAACTGCCAATCCAGATGTAACAAGTGTTGAGATTTGTGATGATGATGAGTTTCTTGTCATCGCTTGTGATGGGATATGGGACTGCATGTCAAACCAACAACTTGTGGATTATGTTGGGGAACAATTGAAACATGAAAGTAAACTCTCGGTGGTTTGTGAGAGAGTGTTTGAAAGGTGCTTGGCACCCTCGACAGGTGGTGAAGGCTGTGACAACATGACAATGATCTTGGTTCAGTTTAAGAAGCCTATTATTTCAACTGCTTCTACTGGGAACAAGCCTCTTTCGTCTAATCCATCACTTGAGAAGGACAAAAGCTCGGGAAAAGCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

367

Amino Acids

40.17

Weight (kDa)

5.31

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 26 - 107 4e-12 Protein phosphatase 2C
PP2C PF00481 139 - 321 2.8e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 952
AccB7I CCANNNNNTGG 1 cut(s) 500
AccII CGCG 1 cut(s) 568
AciI CCGC 1 cut(s) 197
AcsI RAATTY 3 cut(s) 205, 648, 728
AcuI CTGAAG 1 cut(s) 674
AfeI AGCGCT 1 cut(s) 28
AfiI CCNNNNNNNGG 3 cut(s) 99, 500, 962
AhdI GACNNNNNGTC 1 cut(s) 856
AjnI CCWGG 1 cut(s) 243
AluBI AGCT 7 cut(s) 260, 290, 344, 419, 512, 1086, 1097
AluI AGCT 7 cut(s) 260, 290, 344, 419, 512, 1086, 1097
Alw21I GWGCWC 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 1087
Aor51HI AGCGCT 1 cut(s) 28
AoxI GGCC 3 cut(s) 376, 470, 657
ApeKI GCWGC 2 cut(s) 73, 131
ApoI RAATTY 3 cut(s) 205, 648, 728
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 470, 491
AsuHPI GGTGA 4 cut(s) 71, 275, 572, 980
AvaI CYCGRG 1 cut(s) 1087
AvaII GGWCC 1 cut(s) 491
BanI GGYRCC 1 cut(s) 952
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 60, 118
BccI CCATC 8 cut(s) 4, 50, 173, 295, 324, 482, 833, 1072
BciT130I CCWGG 1 cut(s) 245
BclI TGATCA 2 cut(s) 439, 448
BfaI CTAG 2 cut(s) 257, 341
BfmI CTRYAG 1 cut(s) 281
BfoI RGCGCY 1 cut(s) 30
BglII AGATCT 1 cut(s) 142
BisI GCNGC 2 cut(s) 74, 132
BlsI GCNGC 2 cut(s) 75, 133
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 491
BmeRI GACNNNNNGTC 1 cut(s) 856
BmeT110I CYCGRG 1 cut(s) 1087
BmgT120I GGNCC 2 cut(s) 470, 491
BmiI GGNNCC 2 cut(s) 471, 954
BmrFI CCNGG 1 cut(s) 245
BmrI ACTGGG 1 cut(s) 1047
BmsI GCATC 2 cut(s) 112, 303
BmuI ACTGGG 1 cut(s) 1047
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 1091
BsaJI CCNNGG 1 cut(s) 114
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 500, 962
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 1042
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 4 cut(s) 306, 310, 335, 525
BseLI CCNNNNNNNGG 3 cut(s) 99, 500, 962
BseMII CTCAG 3 cut(s) 14, 310, 453
BseNI ACTGG 1 cut(s) 1042
BseRI GAGGAG 1 cut(s) 479
BseXI GCAGC 2 cut(s) 60, 118
Bsh1236I CGCG 1 cut(s) 568
BshFI GGCC 3 cut(s) 378, 472, 659
BshNI GGYRCC 1 cut(s) 952
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 1087
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 1 cut(s) 863
BslI CCNNNNNNNGG 3 cut(s) 99, 500, 962
BsmFI GGGAC 1 cut(s) 863
BsmI GAATGC 1 cut(s) 431
BsnI GGCC 3 cut(s) 378, 472, 659
BsoBI CYCGRG 1 cut(s) 1087
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 5 cut(s) 142, 439, 448, 613, 993
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 1 cut(s) 197
BspANI GGCC 3 cut(s) 378, 472, 659
BspCNI CTCAG 3 cut(s) 15, 309, 454
BspFNI CGCG 1 cut(s) 568
BspLI GGNNCC 2 cut(s) 471, 954
BspMAI CTGCAG 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 952
BsrFI RCCGGY 1 cut(s) 659
BsrI ACTGG 1 cut(s) 1042
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 5 cut(s) 142, 439, 448, 613, 993
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 245
BstDEI CTNAG 5 cut(s) 23, 296, 405, 420, 462
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 4 cut(s) 306, 310, 335, 525
BstFNI CGCG 1 cut(s) 568
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 5 cut(s) 145, 442, 451, 616, 996
BstMBI GATC 5 cut(s) 142, 439, 448, 613, 993
BstMWI GCNNNNNNNGC 4 cut(s) 103, 131, 257, 518
BstNI CCWGG 1 cut(s) 245
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 281
BstUI CGCG 1 cut(s) 568
BstV1I GCAGC 2 cut(s) 60, 118
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuRI GGCC 3 cut(s) 378, 472, 659
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 814
BtsCI GGATG 4 cut(s) 306, 310, 335, 525
BtsIMutI CAGTG 1 cut(s) 458
CfoI GCGC 1 cut(s) 29
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 2 cut(s) 470, 491
CviAII CATG 7 cut(s) 94, 115, 128, 185, 856, 902, 985
DdeI CTNAG 5 cut(s) 23, 296, 405, 420, 462
DpnI GATC 5 cut(s) 144, 441, 450, 615, 995
DpnII GATC 5 cut(s) 142, 439, 448, 613, 993
DriI GACNNNNNGTC 1 cut(s) 856
Eam1105I GACNNNNNGTC 1 cut(s) 856
Eco130I CCWWGG 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 378
Eco47I GGWCC 1 cut(s) 491
Eco47III AGCGCT 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 674
Eco88I CYCGRG 1 cut(s) 1087
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 2 cut(s) 648, 728
EcoRII CCWGG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 7 cut(s) 97, 118, 131, 188, 859, 905, 988
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FaqI GGGAC 1 cut(s) 863
FatI CATG 7 cut(s) 93, 114, 127, 184, 855, 901, 984
FbaI TGATCA 2 cut(s) 439, 448
Fnu4HI GCNGC 2 cut(s) 74, 132
FokI GGATG 4 cut(s) 313, 317, 342, 512
Fsp4HI GCNGC 2 cut(s) 74, 132
FspBI CTAG 2 cut(s) 257, 341
GlaI GCGC 1 cut(s) 28
GluI GCNGC 2 cut(s) 74, 132
GsuI CTGGAG 1 cut(s) 26
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 3 cut(s) 378, 472, 659
HapII CCGG 1 cut(s) 660
HhaI GCGC 1 cut(s) 29
Hin1II CATG 7 cut(s) 97, 118, 131, 188, 859, 905, 988
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 2 cut(s) 64, 563
HpaII CCGG 1 cut(s) 660
HphI GGTGA 4 cut(s) 71, 275, 572, 980
Hpy166II GTNNAC 2 cut(s) 320, 911
Hpy188I TCNGA 7 cut(s) 24, 33, 52, 463, 481, 530, 654
Hpy188III TCNNGA 6 cut(s) 140, 146, 298, 501, 782, 1089
Hpy8I GTNNAC 2 cut(s) 320, 911
HpyAV CCTTC 6 cut(s) 380, 580, 634, 649, 965, 1069
HpyCH4V TGCA 4 cut(s) 97, 283, 431, 855
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 131, 257, 518
HpyF3I CTNAG 5 cut(s) 23, 296, 405, 420, 462
Hsp92II CATG 7 cut(s) 97, 118, 131, 188, 859, 905, 988
HspAI GCGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 439, 448
Kzo9I GATC 5 cut(s) 142, 439, 448, 613, 993
LmnI GCTCC 1 cut(s) 34
Lsp1109I GCAGC 2 cut(s) 60, 118
LweI GCATC 2 cut(s) 112, 303
MaeI CTAG 2 cut(s) 257, 341
MaeIII GTNAC 3 cut(s) 769, 787, 977
MalI GATC 5 cut(s) 144, 441, 450, 615, 995
MboI GATC 5 cut(s) 142, 439, 448, 613, 993
MboII GAAGA 3 cut(s) 65, 131, 450
MfeI CAATTG 2 cut(s) 746, 893
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 7 cut(s) 151, 205, 444, 648, 728, 746, 893
MmeI TCCRAC 1 cut(s) 658
MnlI CCTC 9 cut(s) 100, 182, 320, 457, 460, 483, 576, 967, 1059
MseI TTAA 1 cut(s) 1008
MslI CAYNNNNRTG 1 cut(s) 989
MspI CCGG 1 cut(s) 660
MspR9I CCNGG 1 cut(s) 245
MunI CAATTG 2 cut(s) 746, 893
Mva1269I GAATGC 1 cut(s) 431
MvaI CCWGG 1 cut(s) 245
MvnI CGCG 1 cut(s) 568
MwoI GCNNNNNNNGC 4 cut(s) 103, 131, 257, 518
NcoI CCATGG 1 cut(s) 114
NdeII GATC 5 cut(s) 142, 439, 448, 613, 993
NlaIII CATG 7 cut(s) 97, 118, 131, 188, 859, 905, 988
NlaIV GGNNCC 2 cut(s) 471, 954
NmuCI GTSAC 1 cut(s) 977
NspI RCATGY 1 cut(s) 859
PceI AGGCCT 1 cut(s) 378
PctI GAATGC 1 cut(s) 431
PfeI GAWTC 2 cut(s) 64, 563
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 2 cut(s) 75, 133
Psp6I CCWGG 1 cut(s) 243
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 471, 954
PspPI GGNCC 2 cut(s) 470, 491
PstI CTGCAG 1 cut(s) 285
PsuI RGATCY 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 989
SaqAI TTAA 1 cut(s) 1008
SatI GCNGC 2 cut(s) 74, 132
Sau3AI GATC 5 cut(s) 142, 439, 448, 613, 993
Sau96I GGNCC 2 cut(s) 470, 491
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 240
SfaNI GCATC 2 cut(s) 112, 303
SfcI CTRYAG 1 cut(s) 281
SinI GGWCC 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 989
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
Sse9I AATT 7 cut(s) 151, 205, 444, 648, 728, 746, 893
SseBI AGGCCT 1 cut(s) 378
SsiI CCGC 1 cut(s) 197
SspMI CTAG 2 cut(s) 257, 341
StuI AGGCCT 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 114
TaqI TCGA 3 cut(s) 147, 581, 959
TasI AATT 7 cut(s) 151, 205, 444, 648, 728, 746, 893
TfiI GAWTC 2 cut(s) 64, 563
Tru1I TTAA 1 cut(s) 1008
Tru9I TTAA 1 cut(s) 1008
TscAI CASTG 1 cut(s) 458
TseFI GTSAC 1 cut(s) 977
TseI GCWGC 2 cut(s) 73, 131
Tsp45I GTSAC 1 cut(s) 977
TspDTI ATGAA 2 cut(s) 382, 918
TspRI CASTG 1 cut(s) 458
Van91I CCANNNNNTGG 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 491
XapI RAATTY 3 cut(s) 205, 648, 728
XceI RCATGY 1 cut(s) 859
XspI CTAG 2 cut(s) 257, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.