pycom15g03860

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000743
Physical Location & Seq
Reverse (-)
2369102 .. 2372516
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g03860.9

Sequence Viewer

Length: 1005 bp
ATGCAAGGATGGCGTGCCACAATGGAGGATGCTCATGCAGCATATCCTGATCTGGATGCATCCACCTCATTCTTTGGTGTTTATGATGGCCATGGAGGCAAGGTAGTTGCTAAGTTCTGTGCAAAATATCTGCACCAACAAGTGCTCAAGAATGAAGCATATGCGGCTGGAGACATAGGAACTTCGGTTCAAAAAGCTTTTTTCAGAATGGATGAAATGATGCGCGGACAAAAGGGTTGGAGAGAGTTAGCTGTTTTGGGAGATAAGATAAACAAGTTTACTGGCATGATAGAAGGGTTGATTTGGTCTCCGAGGAGCAGTGATGGTAACGATCAAGCTGATGATTGGGCTTTTGAGGAGGGGCCTCACTCTGATTTTACTGGTCCAACTTCTGGGTGCACAGCCTGTGTTGCAATTCTTAGAAACAAGCAACTTGTTGTGGCAAATGCTGGTGATTCGCGCTGTGTGATATCAAGGAAGGGTCAGGCTTATAATCTGTCAAGGGACCACAAACCCGATCTTGAGCTTGAGAAGGAGAGGATTTTAAAAGCTGGTGGTTTTATCCATGCAGGACGAGTCAATGGAAGTTTGAATCTTGCAAGAGCTATAGGTGACATGGAATTTAAGCAGAATAAATTTTTGCCAGCTGAAAAACAAATTGTAACTGCCAGTCCGGACATAAACACTGTTGAGCTGTGCGACGACGACGAATTTATTGTGCTAGCATGTGATGGCATCTGGGACTGTATGTCTAGCCAGCAAGTGGTAGATTTTGTACATGAACAACTGCTCTCGGAAAGCAAGCTATCTGTGGTGTGCGAGAGAGCCCTTGATAGGTGCTTGGCGCCATCAACTGCTGATGGTGAGGGATGCGATAACATGACCATGATCGTGGTGCAGTTCAAGAAGCCTGAACAGTCGACTGAATCCGAGGGTGGGCAATCCTCACCAACTGAAGTAGCAGCCGGAAGTTATTCAAAGGGGAAGTCATTCAAAGGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

335

Amino Acids

36.47

Weight (kDa)

5.13

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 844
AccB7I CCANNNNNTGG 2 cut(s) 392, 763
AccI GTMKAC 1 cut(s) 920
AccII CGCG 2 cut(s) 225, 460
AccIII TCCGGA 1 cut(s) 673
AciI CCGC 2 cut(s) 164, 225
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 3 cut(s) 620, 635, 710
AcuI CTGAAG 1 cut(s) 975
AcyI GRCGYC 1 cut(s) 845
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 4 cut(s) 392, 763, 834, 936
AgsI TTSAA 5 cut(s) 191, 592, 904, 978, 994
AhdI GACNNNNNGTC 1 cut(s) 748
AloI GAACNNNNNNTCC 2 cut(s) 171, 203
AluBI AGCT 9 cut(s) 197, 251, 338, 526, 551, 605, 647, 694, 805
AluI AGCT 9 cut(s) 197, 251, 338, 526, 551, 605, 647, 694, 805
Alw21I GWGCWC 2 cut(s) 147, 401
Alw26I GTCTC 2 cut(s) 165, 312
Alw44I GTGCAC 1 cut(s) 397
Aor13HI TCCGGA 1 cut(s) 673
AoxI GGCC 2 cut(s) 88, 362
ApaLI GTGCAC 1 cut(s) 397
ApeKI GCWGC 2 cut(s) 38, 962
ApoI RAATTY 3 cut(s) 620, 635, 710
ArsI GACNNNNNNTTYG 2 cut(s) 971, 1003
Asp700I GAANNNNTTC 2 cut(s) 973, 989
AspLEI GCGC 3 cut(s) 225, 462, 847
AspS9I GGNCC 3 cut(s) 362, 383, 505
AsuHPI GGTGA 4 cut(s) 464, 623, 875, 939
AsuNHI GCTAGC 1 cut(s) 721
AvaII GGWCC 2 cut(s) 383, 505
BaeGI GKGCMC 1 cut(s) 401
BalI TGGCCA 1 cut(s) 90
BanI GGYRCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 829
Bbv12I GWGCWC 2 cut(s) 147, 401
BbvI GCAGC 2 cut(s) 50, 974
BccI CCATC 6 cut(s) 3, 80, 317, 725, 854, 856
BcoDI GTCTC 2 cut(s) 165, 312
BfaI CTAG 2 cut(s) 722, 753
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 848
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 3 cut(s) 39, 165, 963
BlsI GCNGC 3 cut(s) 40, 166, 964
Bme18I GGWCC 2 cut(s) 383, 505
BmeRI GACNNNNNGTC 1 cut(s) 748
BmgT120I GGNCC 3 cut(s) 362, 383, 505
BmiI GGNNCC 3 cut(s) 363, 506, 846
BmsI GCATC 6 cut(s) 19, 46, 68, 210, 744, 860
BmtI GCTAGC 1 cut(s) 725
BpmI CTGGAG 1 cut(s) 189
BpuEI CTTGAG 3 cut(s) 131, 542, 548
BsaHI GRCGYC 1 cut(s) 845
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 3 cut(s) 91, 311, 930
BsaWI WCCGGW 1 cut(s) 673
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 4 cut(s) 392, 763, 834, 936
Bse1I ACTGG 3 cut(s) 286, 385, 669
BseAI TCCGGA 1 cut(s) 673
BseDI CCNNGG 3 cut(s) 91, 311, 930
BseGI GGATG 6 cut(s) 14, 34, 59, 61, 217, 875
BseLI CCNNNNNNNGG 4 cut(s) 392, 763, 834, 936
BseNI ACTGG 3 cut(s) 286, 385, 669
BseRI GAGGAG 2 cut(s) 328, 371
BseSI GKGCMC 1 cut(s) 401
BseXI GCAGC 2 cut(s) 50, 974
BsgI GTGCAG 2 cut(s) 116, 917
Bsh1236I CGCG 2 cut(s) 225, 460
BshFI GGCC 2 cut(s) 90, 364
BshNI GGYRCC 1 cut(s) 844
BsiHKAI GWGCWC 2 cut(s) 147, 401
BsiSI CCGG 2 cut(s) 674, 966
BslFI GGGAC 2 cut(s) 518, 755
BslI CCNNNNNNNGG 4 cut(s) 392, 763, 834, 936
BsmAI GTCTC 2 cut(s) 165, 312
BsmFI GGGAC 2 cut(s) 518, 755
BsnI GGCC 2 cut(s) 90, 364
Bso31I GGTCTC 1 cut(s) 312
Bsp1286I GDGCHC 3 cut(s) 147, 401, 829
Bsp13I TCCGGA 1 cut(s) 673
Bsp1407I TGTACA 1 cut(s) 775
Bsp143I GATC 4 cut(s) 49, 331, 517, 888
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 2 cut(s) 164, 225
BspANI GGCC 2 cut(s) 90, 364
BspEI TCCGGA 1 cut(s) 673
BspFNI CGCG 2 cut(s) 225, 460
BspLI GGNNCC 3 cut(s) 363, 506, 846
BspOI GCTAGC 1 cut(s) 725
BspT107I GGYRCC 1 cut(s) 844
BspTNI GGTCTC 1 cut(s) 312
BsrGI TGTACA 1 cut(s) 775
BsrI ACTGG 3 cut(s) 286, 385, 669
BssECI CCNNGG 3 cut(s) 91, 311, 930
BssMI GATC 4 cut(s) 49, 331, 517, 888
BssNI GRCGYC 1 cut(s) 845
BssT1I CCWWGG 1 cut(s) 91
Bst4CI ACNGT 3 cut(s) 688, 746, 918
BstACI GRCGYC 1 cut(s) 845
BstAUI TGTACA 1 cut(s) 775
BstC8I GCNNGC 5 cut(s) 15, 645, 723, 758, 803
BstDEI CTNAG 2 cut(s) 111, 419
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 6 cut(s) 14, 34, 59, 61, 217, 875
BstFNI CGCG 2 cut(s) 225, 460
BstH2I RGCGCY 1 cut(s) 848
BstHHI GCGC 3 cut(s) 225, 462, 847
BstKTI GATC 4 cut(s) 52, 334, 520, 891
BstMAI GTCTC 2 cut(s) 165, 312
BstMBI GATC 4 cut(s) 49, 331, 517, 888
BstMWI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
BstNSI RCATGY 1 cut(s) 729
BstSFI CTRYAG 1 cut(s) 606
BstSLI GKGCMC 1 cut(s) 401
BstUI CGCG 2 cut(s) 225, 460
BstV1I GCAGC 2 cut(s) 50, 974
BstXI CCANNNNNNTGG 1 cut(s) 892
BsuRI GGCC 2 cut(s) 90, 364
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 6 cut(s) 14, 34, 59, 61, 217, 875
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 325, 684
Cac8I GCNNGC 5 cut(s) 15, 645, 723, 758, 803
CfoI GCGC 3 cut(s) 225, 462, 847
Cfr13I GGNCC 3 cut(s) 362, 383, 505
Csp6I GTAC 1 cut(s) 776
CviAII CATG 9 cut(s) 35, 92, 286, 566, 616, 726, 779, 880, 886
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 2 cut(s) 111, 419
DinI GGCGCC 1 cut(s) 846
DpnI GATC 4 cut(s) 51, 333, 519, 890
DpnII GATC 4 cut(s) 49, 331, 517, 888
DraI TTTAAA 1 cut(s) 546
DriI GACNNNNNGTC 1 cut(s) 748
EaeI YGGCCR 1 cut(s) 88
Eam1105I GACNNNNNGTC 1 cut(s) 748
Eco130I CCWWGG 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 829
Eco31I GGTCTC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 383, 505
Eco57I CTGAAG 1 cut(s) 975
EcoO109I RGGNCCY 1 cut(s) 362
EcoRV GATATC 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 829
EgeI GGCGCC 1 cut(s) 846
EheI GGCGCC 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 9 cut(s) 38, 95, 289, 569, 619, 729, 782, 883, 889
FaqI GGGAC 2 cut(s) 518, 755
FatI CATG 9 cut(s) 34, 91, 285, 565, 615, 725, 778, 879, 885
FauNDI CATATG 2 cut(s) 160, 1001
FblI GTMKAC 1 cut(s) 920
Fnu4HI GCNGC 3 cut(s) 39, 165, 963
FokI GGATG 6 cut(s) 21, 41, 46, 68, 224, 882
FriOI GRGCYC 1 cut(s) 829
Fsp4HI GCNGC 3 cut(s) 39, 165, 963
FspBI CTAG 2 cut(s) 722, 753
GlaI GCGC 3 cut(s) 224, 461, 846
GluI GCNGC 3 cut(s) 39, 165, 963
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 848
HaeIII GGCC 2 cut(s) 90, 364
HapII CCGG 2 cut(s) 674, 966
HhaI GCGC 3 cut(s) 225, 462, 847
Hin1I GRCGYC 1 cut(s) 845
Hin1II CATG 9 cut(s) 38, 95, 289, 569, 619, 729, 782, 883, 889
Hin6I GCGC 3 cut(s) 223, 460, 845
HinP1I GCGC 3 cut(s) 223, 460, 845
HincII GTYRAC 1 cut(s) 921
HindII GTYRAC 1 cut(s) 921
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 4 cut(s) 455, 576, 592, 926
HpaII CCGG 2 cut(s) 674, 966
HphI GGTGA 4 cut(s) 464, 623, 875, 939
Hpy166II GTNNAC 3 cut(s) 279, 399, 921
Hpy188I TCNGA 5 cut(s) 206, 312, 373, 796, 931
Hpy188III TCNNGA 6 cut(s) 47, 53, 148, 521, 674, 904
Hpy8I GTNNAC 3 cut(s) 279, 399, 921
Hpy99I CGWCG 3 cut(s) 704, 707, 710
HpyAV CCTTC 3 cut(s) 287, 472, 526
HpyCH4III ACNGT 3 cut(s) 688, 746, 918
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
HpyF3I CTNAG 2 cut(s) 111, 419
Hsp92I GRCGYC 1 cut(s) 845
Hsp92II CATG 9 cut(s) 38, 95, 289, 569, 619, 729, 782, 883, 889
HspAI GCGC 3 cut(s) 223, 460, 845
KasI GGCGCC 1 cut(s) 844
Kpn2I TCCGGA 1 cut(s) 673
Kzo9I GATC 4 cut(s) 49, 331, 517, 888
LmnI GCTCC 1 cut(s) 315
Lsp1109I GCAGC 2 cut(s) 50, 974
LweI GCATC 6 cut(s) 19, 46, 68, 210, 744, 860
MaeI CTAG 2 cut(s) 722, 753
MaeIII GTNAC 3 cut(s) 326, 611, 661
MalI GATC 4 cut(s) 51, 333, 519, 890
MboI GATC 4 cut(s) 49, 331, 517, 888
MhlI GDGCHC 3 cut(s) 147, 401, 829
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 5 cut(s) 414, 620, 635, 657, 710
MluNI TGGCCA 1 cut(s) 90
Mly113I GGCGCC 1 cut(s) 845
MlyI GAGTC 1 cut(s) 585
MmeI TCCRAC 2 cut(s) 218, 410
Mox20I TGGCCA 1 cut(s) 90
Mph1103I ATGCAT 1 cut(s) 61
MroI TCCGGA 1 cut(s) 673
MroXI GAANNNNTTC 2 cut(s) 973, 989
MscI TGGCCA 1 cut(s) 90
MseI TTAA 2 cut(s) 545, 624
MslI CAYNNNNRTG 2 cut(s) 884, 890
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 2 cut(s) 674, 966
MvnI CGCG 2 cut(s) 225, 460
MwoI GCNNNNNNNGC 5 cut(s) 10, 38, 96, 164, 410
NarI GGCGCC 1 cut(s) 845
NcoI CCATGG 1 cut(s) 91
NdeI CATATG 2 cut(s) 160, 1001
NdeII GATC 4 cut(s) 49, 331, 517, 888
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 9 cut(s) 38, 95, 289, 569, 619, 729, 782, 883, 889
NlaIV GGNNCC 3 cut(s) 363, 506, 846
NmuCI GTSAC 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 1 cut(s) 729
PdmI GAANNNNTTC 2 cut(s) 973, 989
PfeI GAWTC 3 cut(s) 455, 592, 926
PflMI CCANNNNNTGG 2 cut(s) 392, 763
PkrI GCNGC 3 cut(s) 40, 166, 964
PleI GAGTC 1 cut(s) 584
PluTI GGCGCC 1 cut(s) 848
PpsI GAGTC 1 cut(s) 584
PsiI TTATAA 1 cut(s) 492
PspN4I GGNNCC 3 cut(s) 363, 506, 846
PspPI GGNCC 3 cut(s) 362, 383, 505
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
RseI CAYNNNNRTG 2 cut(s) 884, 890
SalI GTCGAC 1 cut(s) 919
SaqAI TTAA 2 cut(s) 545, 624
SatI GCNGC 3 cut(s) 39, 165, 963
Sau3AI GATC 4 cut(s) 49, 331, 517, 888
Sau96I GGNCC 3 cut(s) 362, 383, 505
SchI GAGTC 1 cut(s) 585
SduI GDGCHC 3 cut(s) 147, 401, 829
SfaNI GCATC 6 cut(s) 19, 46, 68, 210, 744, 860
SfcI CTRYAG 1 cut(s) 606
SfoI GGCGCC 1 cut(s) 846
SinI GGWCC 2 cut(s) 383, 505
SmiMI CAYNNNNRTG 2 cut(s) 884, 890
SmlI CTYRAG 3 cut(s) 146, 521, 527
SmoI CTYRAG 3 cut(s) 146, 521, 527
Sse9I AATT 5 cut(s) 414, 620, 635, 657, 710
SsiI CCGC 2 cut(s) 164, 225
SspDI GGCGCC 1 cut(s) 844
SspMI CTAG 2 cut(s) 722, 753
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 3 cut(s) 688, 746, 918
TaqI TCGA 1 cut(s) 920
TasI AATT 5 cut(s) 414, 620, 635, 657, 710
TatI WGTACW 1 cut(s) 775
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 3 cut(s) 455, 592, 926
Tru1I TTAA 2 cut(s) 545, 624
Tru9I TTAA 2 cut(s) 545, 624
TscAI CASTG 2 cut(s) 325, 691
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 2 cut(s) 38, 962
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 3 cut(s) 168, 228, 795
TspRI CASTG 2 cut(s) 325, 691
Van91I CCANNNNNTGG 2 cut(s) 392, 763
VneI GTGCAC 1 cut(s) 397
VpaK11BI GGWCC 2 cut(s) 383, 505
XapI RAATTY 3 cut(s) 620, 635, 710
XceI RCATGY 1 cut(s) 729
XmiI GTMKAC 1 cut(s) 920
XmnI GAANNNNTTC 2 cut(s) 973, 989
XspI CTAG 2 cut(s) 722, 753
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.