pycom17g22840

protein phosphatase 2C 60

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000743
Physical Location & Seq
Reverse (-)
21231296 .. 21234577
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22840.5

Sequence Viewer

Length: 657 bp
ATGCTCGATCAGTGGTCTTCTGAGGAGGGACCTCATTCTGATTTTGATGGACCAAATTCTGGAAGTACAGCTTGTGTTGCTATTATCCGAAACAAACAACTTCTTGTTGCCAATGCTGGTGATTCTCGCTGCGTAATATCCAGGAAGGGTGAGGCTTATAATTTGTCTAAAGATCATAAGCCTGACATTGAGAATGAGAAAGAAAGAATTTTGAAGGCTGGTGGATTTATCCAAGTTGGAAGGGTCAATGGAAGTTTGAACCTGGCAAGAGCAATTGGGGATGCAGAATTCAAGCAGAACAAACAGTTGCCCGTTGAAAAGCAGATTGTGACTGCCAATCCAGACATAAATAGCGTTGAGCTTTGCGATGATGATGAGTTTCTGGTCATAGCTTGCGATGGGATATGGGACTGCATGTCAAGTCAACAACTTGTGGACTATGTCCGGGAACAATTAAAACATGAAAGTAAACTGTCAGTGGTTTGCGAGAGAGTGTTTGAAAGGTGCTTGGCGCCATCATCTGGTGGAGAGGGATGTGACAACATGACAATGATCTTGGTTCAGTTTAACAAACCTGTTGCTTCAGCTGCAGCTGTTGGGAACCAGCCCGTCGTGTCTAATCTGCCATCCGAGAAGGAAAAAACCGCAGCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

219

Amino Acids

23.72

Weight (kDa)

4.91

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 511
AccB7I CCANNNNNTGG 2 cut(s) 59, 521
AciI CCGC 1 cut(s) 645
AcsI RAATTY 3 cut(s) 55, 207, 287
AcuI CTGAAG 1 cut(s) 567
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 2 cut(s) 59, 521
AgsI TTSAA 5 cut(s) 214, 259, 292, 317, 500
AhdI GACNNNNNGTC 1 cut(s) 415
AjnI CCWGG 2 cut(s) 140, 261
AluBI AGCT 5 cut(s) 71, 361, 392, 587, 593
AluI AGCT 5 cut(s) 71, 361, 392, 587, 593
ApeKI GCWGC 4 cut(s) 129, 587, 590, 647
ApoI RAATTY 3 cut(s) 55, 207, 287
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 2 cut(s) 29, 50
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 2 cut(s) 131, 161
AvaII GGWCC 2 cut(s) 29, 50
BanI GGYRCC 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 9
BbvI GCAGC 3 cut(s) 116, 574, 602
BccI CCATC 4 cut(s) 41, 392, 523, 634
BciT130I CCWGG 2 cut(s) 142, 263
BcnI CCSGG 1 cut(s) 446
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 515
BisI GCNGC 4 cut(s) 130, 588, 591, 648
BlsI GCNGC 4 cut(s) 131, 589, 592, 649
Bme1390I CCNGG 3 cut(s) 142, 263, 446
Bme18I GGWCC 2 cut(s) 29, 50
BmeRI GACNNNNNGTC 1 cut(s) 415
BmgT120I GGNCC 2 cut(s) 29, 50
BmiI GGNNCC 3 cut(s) 30, 513, 602
BmrFI CCNGG 3 cut(s) 142, 263, 446
BmsI GCATC 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 9
BpuMI CCSGG 1 cut(s) 446
BsaHI GRCGYC 1 cut(s) 512
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 521
BseBI CCWGG 2 cut(s) 142, 263
BseGI GGATG 3 cut(s) 286, 539, 626
BseLI CCNNNNNNNGG 2 cut(s) 59, 521
BseMII CTCAG 1 cut(s) 12
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 3 cut(s) 116, 574, 602
BshNI GGYRCC 1 cut(s) 511
BsiSI CCGG 1 cut(s) 445
BslFI GGGAC 2 cut(s) 42, 422
BslI CCNNNNNNNGG 2 cut(s) 59, 521
BsmFI GGGAC 2 cut(s) 42, 422
Bsp143I GATC 3 cut(s) 7, 172, 552
BspACI CCGC 1 cut(s) 645
BspCNI CTCAG 1 cut(s) 13
BspLI GGNNCC 3 cut(s) 30, 513, 602
BspMAI CTGCAG 1 cut(s) 592
BspT107I GGYRCC 1 cut(s) 511
BssMI GATC 3 cut(s) 7, 172, 552
BssNI GRCGYC 1 cut(s) 512
Bst2UI CCWGG 2 cut(s) 142, 263
Bst4CI ACNGT 2 cut(s) 306, 474
BstACI GRCGYC 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 1 cut(s) 21
BstF5I GGATG 3 cut(s) 286, 539, 626
BstH2I RGCGCY 1 cut(s) 515
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 3 cut(s) 10, 175, 555
BstMBI GATC 3 cut(s) 7, 172, 552
BstMWI GCNNNNNNNGC 2 cut(s) 77, 587
BstNI CCWGG 2 cut(s) 142, 263
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 3 cut(s) 140, 261, 444
BstSFI CTRYAG 1 cut(s) 588
BstV1I GCAGC 3 cut(s) 116, 574, 602
BstV2I GAAGAC 1 cut(s) 9
BtgZI GCGATG 2 cut(s) 381, 411
BtsCI GGATG 3 cut(s) 286, 539, 626
BtsIMutI CAGTG 2 cut(s) 17, 483
Cac8I GCNNGC 1 cut(s) 394
CfoI GCGC 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 29, 50
Csp6I GTAC 1 cut(s) 66
CviAII CATG 3 cut(s) 415, 461, 544
CviJI RGCY 9 cut(s) 71, 155, 181, 218, 361, 392, 587, 593, 607
CviKI_1 RGCY 9 cut(s) 71, 155, 181, 218, 361, 392, 587, 593, 607
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 1 cut(s) 21
DinI GGCGCC 1 cut(s) 513
DpnI GATC 3 cut(s) 9, 174, 554
DpnII GATC 3 cut(s) 7, 172, 552
DriI GACNNNNNGTC 1 cut(s) 415
Eam1105I GACNNNNNGTC 1 cut(s) 415
Eco47I GGWCC 2 cut(s) 29, 50
Eco57I CTGAAG 1 cut(s) 567
EcoO109I RGGNCCY 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 140, 261
EgeI GGCGCC 1 cut(s) 513
EheI GGCGCC 1 cut(s) 513
FaeI CATG 3 cut(s) 418, 464, 547
FaiI YATR 9 cut(s) 159, 177, 347, 389, 406, 416, 441, 462, 545
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 2 cut(s) 42, 422
FatI CATG 3 cut(s) 414, 460, 543
Fnu4HI GCNGC 4 cut(s) 130, 588, 591, 648
FokI GGATG 3 cut(s) 293, 546, 613
Fsp4HI GCNGC 4 cut(s) 130, 588, 591, 648
GlaI GCGC 1 cut(s) 513
GluI GCNGC 4 cut(s) 130, 588, 591, 648
HaeII RGCGCY 1 cut(s) 515
HapII CCGG 1 cut(s) 445
HhaI GCGC 1 cut(s) 514
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 3 cut(s) 418, 464, 547
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HincII GTYRAC 1 cut(s) 425
HindII GTYRAC 1 cut(s) 425
HinfI GANTC 1 cut(s) 122
HpaII CCGG 1 cut(s) 445
HphI GGTGA 2 cut(s) 131, 161
Hpy166II GTNNAC 3 cut(s) 425, 436, 470
Hpy188I TCNGA 4 cut(s) 22, 40, 89, 631
Hpy188III TCNNGA 2 cut(s) 60, 341
Hpy8I GTNNAC 3 cut(s) 425, 436, 470
Hpy99I CGWCG 1 cut(s) 614
HpyAV CCTTC 4 cut(s) 139, 208, 234, 628
HpyCH4III ACNGT 2 cut(s) 306, 474
HpyCH4V TGCA 3 cut(s) 284, 414, 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 587
HpyF3I CTNAG 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 3 cut(s) 418, 464, 547
HspAI GCGC 1 cut(s) 512
KasI GGCGCC 1 cut(s) 511
Kzo9I GATC 3 cut(s) 7, 172, 552
Lsp1109I GCAGC 3 cut(s) 116, 574, 602
LweI GCATC 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 328, 536
MalI GATC 3 cut(s) 9, 174, 554
MboI GATC 3 cut(s) 7, 172, 552
MboII GAAGA 1 cut(s) 9
MfeI CAATTG 1 cut(s) 273
MluCI AATT 6 cut(s) 55, 160, 207, 273, 287, 452
Mly113I GGCGCC 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 5 cut(s) 16, 19, 42, 145, 523
MseI TTAA 2 cut(s) 455, 567
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 2 cut(s) 587, 593
MspI CCGG 1 cut(s) 445
MspR9I CCNGG 3 cut(s) 142, 263, 446
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 2 cut(s) 142, 263
MwoI GCNNNNNNNGC 2 cut(s) 77, 587
NarI GGCGCC 1 cut(s) 512
NciI CCSGG 1 cut(s) 446
NdeII GATC 3 cut(s) 7, 172, 552
NlaIII CATG 3 cut(s) 418, 464, 547
NlaIV GGNNCC 3 cut(s) 30, 513, 602
NmuCI GTSAC 2 cut(s) 328, 536
NspI RCATGY 1 cut(s) 418
PfeI GAWTC 1 cut(s) 122
PflFI GACNNNGTC 1 cut(s) 440
PflMI CCANNNNNTGG 2 cut(s) 59, 521
PfoI TCCNGGA 2 cut(s) 140, 444
PkrI GCNGC 4 cut(s) 131, 589, 592, 649
PluTI GGCGCC 1 cut(s) 515
PpuMI RGGWCCY 1 cut(s) 29
PsiI TTATAA 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 140, 261
PspGI CCWGG 2 cut(s) 140, 261
PspN4I GGNNCC 3 cut(s) 30, 513, 602
PspPI GGNCC 2 cut(s) 29, 50
PspPPI RGGWCCY 1 cut(s) 29
PstI CTGCAG 1 cut(s) 592
PsyI GACNNNGTC 1 cut(s) 440
PvuII CAGCTG 2 cut(s) 587, 593
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 2 cut(s) 455, 567
SatI GCNGC 4 cut(s) 130, 588, 591, 648
Sau3AI GATC 3 cut(s) 7, 172, 552
Sau96I GGNCC 2 cut(s) 29, 50
ScrFI CCNGG 3 cut(s) 142, 263, 446
SetI ASST 9 cut(s) 34, 73, 264, 363, 394, 506, 577, 589, 595
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 1 cut(s) 588
SfoI GGCGCC 1 cut(s) 513
SinI GGWCC 2 cut(s) 29, 50
SmiMI CAYNNNNRTG 1 cut(s) 548
Sse9I AATT 6 cut(s) 55, 160, 207, 273, 287, 452
SsiI CCGC 1 cut(s) 645
SspDI GGCGCC 1 cut(s) 511
StyD4I CCNGG 3 cut(s) 140, 261, 444
TaaI ACNGT 2 cut(s) 306, 474
TaqI TCGA 1 cut(s) 6
TasI AATT 6 cut(s) 55, 160, 207, 273, 287, 452
TatI WGTACW 1 cut(s) 65
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 2 cut(s) 455, 567
Tru9I TTAA 2 cut(s) 455, 567
TscAI CASTG 2 cut(s) 17, 483
TseFI GTSAC 2 cut(s) 328, 536
TseI GCWGC 4 cut(s) 129, 587, 590, 647
Tsp45I GTSAC 2 cut(s) 328, 536
TspDTI ATGAA 1 cut(s) 477
TspRI CASTG 2 cut(s) 17, 483
Tth111I GACNNNGTC 1 cut(s) 440
Van91I CCANNNNNTGG 2 cut(s) 59, 521
VpaK11BI GGWCC 2 cut(s) 29, 50
XapI RAATTY 3 cut(s) 55, 207, 287
XceI RCATGY 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.