MD08G1055500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000743
Physical Location & Seq
Forward (+)
4314152 .. 4314619
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1055500.v1.1.491

Sequence Viewer

Length: 468 bp
ATGCAATTCAATCATACATTGGGATGCAGAAACTTGGTTTCTCTGCTGATAACTACGCATTTCCCCTTCTGCTCAAAGCAGCAGGTAATACTTCTTTTGCGTATCGGGTTATCGCTCCATGGCCGGACAGTTAAAACCGGTTTCTGCGACCATCTTCTCGTCCAAACTGCTTTGCTGAAAGACTCCTCGTTAATGCTCGTAAGGTTCGATAAAATGCCCGAGAGAGATGTGGTAGCCTGGAACTCCATGCTAGATGTGTATGCTTCTAGCGGCCAGATGGATAACGCAATGGAAATTTTCGATACAATGCCGTCACAAGACCTTGCATCCTTCAATATAATGATCTCCGGGTTTGCAGGCGCAAGAAGCGTGACCTCTGCCAGAAGTATTTTCGATCGAATTCCTGAGAAAGATGTTGTCTCATGGAACGATGATATTGGCGTGCACGAACGGTGGAGACACGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

156

Amino Acids

17.52

Weight (kDa)

6.57

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 119 4.3e-09 PPR repeat family
PPR PF01535 78 - 105 4.7e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 73
AciI CCGC 1 cut(s) 270
AcoI YGGCCR 2 cut(s) 121, 271
AcsI RAATTY 2 cut(s) 294, 399
AgeI ACCGGT 1 cut(s) 137
AgsI TTSAA 2 cut(s) 10, 334
AjnI CCWGG 1 cut(s) 236
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 2 cut(s) 424, 451
Alw44I GTGCAC 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 2 cut(s) 121, 271
ApaLI GTGCAC 1 cut(s) 443
ApeKI GCWGC 1 cut(s) 79
ApoI RAATTY 2 cut(s) 294, 399
AsiGI ACCGGT 1 cut(s) 137
AspLEI GCGC 1 cut(s) 362
AsuC2I CCSGG 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 91
BccI CCATC 2 cut(s) 159, 271
BceAI ACGGC 1 cut(s) 295
BciT130I CCWGG 1 cut(s) 238
BcnI CCSGG 1 cut(s) 349
BcoDI GTCTC 2 cut(s) 424, 451
BfaI CTAG 2 cut(s) 251, 267
BfuAI ACCTGC 1 cut(s) 73
BisI GCNGC 2 cut(s) 80, 271
BlsI GCNGC 2 cut(s) 81, 272
Bme1390I CCNGG 2 cut(s) 238, 349
BmeT110I CYCGRG 1 cut(s) 218
BmrFI CCNGG 2 cut(s) 238, 349
BmsI GCATC 2 cut(s) 14, 335
BpuMI CCSGG 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 137
Bse118I RCCGGY 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 2 cut(s) 29, 326
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 175
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 123, 273
BshTI ACCGGT 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 3 cut(s) 124, 138, 348
BsmAI GTCTC 2 cut(s) 424, 451
BsnI GGCC 2 cut(s) 123, 273
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 2 cut(s) 342, 394
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 1 cut(s) 270
BspANI GGCC 2 cut(s) 123, 273
BspCNI CTCAG 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 2 cut(s) 342, 394
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 130, 453
BstC8I GCNNGC 2 cut(s) 358, 443
BstDEI CTNAG 1 cut(s) 405
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 2 cut(s) 29, 326
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 2 cut(s) 345, 397
BstMAI GTCTC 2 cut(s) 424, 451
BstMBI GATC 2 cut(s) 342, 394
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 1 cut(s) 366
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 236, 347
BstSLI GKGCMC 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 91
BsuRI GGCC 2 cut(s) 123, 273
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 29, 326
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 358, 443
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 137
CspAI ACCGGT 1 cut(s) 137
CviAII CATG 3 cut(s) 119, 247, 423
CviJI RGCY 4 cut(s) 123, 236, 273, 465
CviKI_1 RGCY 4 cut(s) 123, 236, 273, 465
DdeI CTNAG 1 cut(s) 405
DpnI GATC 2 cut(s) 344, 396
DpnII GATC 2 cut(s) 342, 394
EaeI YGGCCR 2 cut(s) 121, 271
Eco130I CCWWGG 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 218
EcoRI GAATTC 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 3 cut(s) 122, 250, 426
FaiI YATR 6 cut(s) 15, 120, 248, 261, 338, 424
FatI CATG 3 cut(s) 118, 246, 422
Fnu4HI GCNGC 2 cut(s) 80, 271
FokI GGATG 2 cut(s) 36, 313
Fsp4HI GCNGC 2 cut(s) 80, 271
FspBI CTAG 2 cut(s) 251, 267
GlaI GCGC 1 cut(s) 361
GluI GCNGC 2 cut(s) 80, 271
HaeIII GGCC 2 cut(s) 123, 273
HapII CCGG 3 cut(s) 124, 138, 348
HhaI GCGC 1 cut(s) 362
Hin1II CATG 3 cut(s) 122, 250, 426
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HinfI GANTC 1 cut(s) 182
HpaII CCGG 3 cut(s) 124, 138, 348
Hpy166II GTNNAC 1 cut(s) 445
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 1 cut(s) 445
HpyAV CCTTC 2 cut(s) 76, 340
HpyCH4III ACNGT 2 cut(s) 130, 453
HpyCH4V TGCA 5 cut(s) 4, 27, 326, 356, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 366
HpyF3I CTNAG 1 cut(s) 405
Hsp92II CATG 3 cut(s) 122, 250, 426
HspAI GCGC 1 cut(s) 360
Kzo9I GATC 2 cut(s) 342, 394
LmnI GCTCC 1 cut(s) 120
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 2 cut(s) 14, 335
MaeI CTAG 2 cut(s) 251, 267
MaeIII GTNAC 2 cut(s) 312, 370
MalI GATC 2 cut(s) 344, 396
MboI GATC 2 cut(s) 342, 394
MboII GAAGA 1 cut(s) 146
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 3 cut(s) 5, 294, 399
MlyI GAGTC 1 cut(s) 176
MnlI CCTC 2 cut(s) 196, 385
MseI TTAA 2 cut(s) 132, 191
MslI CAYNNNNRTG 1 cut(s) 22
MspI CCGG 3 cut(s) 124, 138, 348
MspR9I CCNGG 2 cut(s) 238, 349
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 366
NciI CCSGG 1 cut(s) 349
NcoI CCATGG 1 cut(s) 118
NdeII GATC 2 cut(s) 342, 394
NlaIII CATG 3 cut(s) 122, 250, 426
NmuCI GTSAC 2 cut(s) 312, 370
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PinAI ACCGGT 1 cut(s) 137
PkrI GCNGC 2 cut(s) 81, 272
Ple19I CGATCG 1 cut(s) 397
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PvuI CGATCG 1 cut(s) 397
RseI CAYNNNNRTG 1 cut(s) 22
SaqAI TTAA 2 cut(s) 132, 191
SatI GCNGC 2 cut(s) 80, 271
Sau3AI GATC 2 cut(s) 342, 394
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 2 cut(s) 238, 349
SduI GDGCHC 1 cut(s) 447
SetI ASST 4 cut(s) 87, 206, 324, 377
SfaNI GCATC 2 cut(s) 14, 335
SmiMI CAYNNNNRTG 1 cut(s) 22
Sse9I AATT 3 cut(s) 5, 294, 399
SsiI CCGC 1 cut(s) 270
SspMI CTAG 2 cut(s) 251, 267
StyD4I CCNGG 2 cut(s) 236, 347
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 2 cut(s) 130, 453
TaqI TCGA 4 cut(s) 207, 300, 393, 397
TasI AATT 3 cut(s) 5, 294, 399
TauI GCSGC 1 cut(s) 273
Tru1I TTAA 2 cut(s) 132, 191
Tru9I TTAA 2 cut(s) 132, 191
TseFI GTSAC 2 cut(s) 312, 370
TseI GCWGC 1 cut(s) 79
Tsp45I GTSAC 2 cut(s) 312, 370
VneI GTGCAC 1 cut(s) 443
XapI RAATTY 2 cut(s) 294, 399
XspI CTAG 2 cut(s) 251, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.