FvH4_6g28470

protein phosphatase 2C 60

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000743
Physical Location & Seq
Forward (+)
21934484 .. 21942662
8179 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28470.t6

Sequence Viewer

Length: 1464 bp
ATGTGCATGATTGATTGGATTATTAGATTGCTGGATATGGGAATATATTTGAGCGCTCCGAAAACCGATAAGGTATCAGAAGATGGTGAGAATGATAGACTTCGATTCGGGGCTTCGTCTATGCAGGGGTGGCGTTCCACCATGGAAGATGCTCATGCTGCTTTGTTAGACCTCGACAATTCAACATCTTTCTTTGGTGTTTATGATGGTCATGGAGGTAAAGCTGTGGCAAATTTCTGCGCCAAGTATCTCCACTTACAAGTGCTTAAACAGGAAGCATATCTAACTGGCGATTTAGGCACTTCCTTACAGAAAGCTTTTCTCAGGATGGATGAGATGATGCGTGGACAGAGAGGATGGAGAGAACTAGCTGTATTGGGTGATAAGATAGATAAAGTTTCGGGCCTTATTGAAGGGTTCATCTTTACTCCCAAGAGTGCTGAAGCCAAGAGAAGTGCATTCAATGATCAAATGGATCAGTGGTCATCTGAGGAGGGGCCTCACTCTGATTTTGATGGACCAACCTCTGGAAGCACAGCTTGTGTCGCTATCATCCGAAACAATCAACTTGTTGTTGCCAATGCTGGTGATTCGCGTTGTGTAATATCCAGAAAGGGTCAGGCATATCCTTTGTCTAAAGATCATAAGCCTGACATTGAGAATGAGAAGGAAAGAATTCTGAAGGCTGGTGGATTTATCCAAGTTGGACGGGTCAATGGAAGTTTAAACTTGGCAAGAGCTATTGGTGATGTGGAATTCAAGCAAAACAAACACTTGCCTGTTGAAAAGCAGATTGTAACTGCCAATCCAGATGTACAAAGTGTTGAGATTTGTGATGATGATGAGTTTCTTGTCATAGCTTGTGATGGAATATGGGACTGCATGTCAAGCCAACAACTTGTGGATTATGTTCGGGAACAATTGAAGCATGAAAATAAACTGTCGGTGGTTTGTGAGCGAGTGTTTGAAAGGTGCTTGGCGCCCTCAACAGGCGGAGAAGGATGTGACAACATGACAATGATCTTGGTTCAGTTTAAGAATCCTGTTAATTCAACTGCTTGTATTGGAAACAACCCTATGTCATCTGATCCATCACCTGAGAAGGCTTCTACTGGGAACCAGCCTCTCTTGTCTGATCCATCACTAGAGAAGGTATCTACTGGGAACAAGCCTCTCTCGTCTGATCCATCACCTGAGAAGGCTTCTACAGGGAACAAGTTTCCGTCTGATCCATCACCGGAGAAGGCTTCTACAGGGAACAAGCCTCTCTCGTCTGATACATCACCCGAGAAGGCTTCTACAGGGAACAAGCCTCTCTCGTCTGATCCATCACCCGAGAAGGCTTCCACTGGGAACAAGCCTCTCTCGTCTGATCCATCACTTGAGAAGACATACACTGGGAATAAGCCTCATTCATCTGATCCATCACCTGAGAAGGACAAAAGCTTGGGAAAAGCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

488

Amino Acids

52.86

Weight (kDa)

5.32

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 42 - 123 2.4e-12 Protein phosphatase 2C
PP2C PF00481 156 - 337 4.8e-61 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 979
AccB7I CCANNNNNTGG 1 cut(s) 527
AccII CGCG 1 cut(s) 595
AciI CCGC 1 cut(s) 993
AclWI GGATC 8 cut(s) 483, 1082, 1130, 1178, 1223, 1319, 1367, 1415
AcsI RAATTY 3 cut(s) 232, 675, 755
AcuI CTGAAG 2 cut(s) 462, 701
AcyI GRCGYC 1 cut(s) 980
AfaI GTAC 1 cut(s) 816
AfeI AGCGCT 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 527, 989
AgsI TTSAA 8 cut(s) 183, 413, 463, 760, 785, 925, 968, 1053
AhdI GACNNNNNGTC 1 cut(s) 883
AluBI AGCT 8 cut(s) 224, 317, 371, 539, 740, 860, 1446, 1457
AluI AGCT 8 cut(s) 224, 317, 371, 539, 740, 860, 1446, 1457
AlwI GGATC 8 cut(s) 483, 1082, 1130, 1178, 1223, 1319, 1367, 1415
Ama87I CYCGRG 2 cut(s) 1286, 1334
Aor51HI AGCGCT 1 cut(s) 55
AoxI GGCC 2 cut(s) 403, 497
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 3 cut(s) 232, 675, 755
Asp700I GAANNNNTTC 1 cut(s) 675
AspLEI GCGC 3 cut(s) 56, 242, 982
AspS9I GGNCC 3 cut(s) 403, 497, 518
AvaI CYCGRG 2 cut(s) 1286, 1334
AvaII GGWCC 1 cut(s) 518
BanI GGYRCC 1 cut(s) 979
BbsI GAAGAC 1 cut(s) 1394
BbvI GCAGC 1 cut(s) 145
BclI TGATCA 1 cut(s) 466
BfaI CTAG 3 cut(s) 368, 1145, 1458
BfmI CTRYAG 3 cut(s) 1206, 1251, 1299
BfoI RGCGCY 2 cut(s) 57, 983
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 518
BmeRI GACNNNNNGTC 1 cut(s) 883
BmeT110I CYCGRG 2 cut(s) 1286, 1334
BmgT120I GGNCC 3 cut(s) 403, 497, 518
BmiI GGNNCC 3 cut(s) 498, 981, 1118
BmrI ACTGGG 4 cut(s) 1122, 1170, 1359, 1407
BmsI GCATC 2 cut(s) 139, 330
BmuI ACTGGG 4 cut(s) 1122, 1170, 1359, 1407
BpiI GAAGAC 1 cut(s) 1394
BpuEI CTTGAG 1 cut(s) 1403
BsaHI GRCGYC 1 cut(s) 980
BsaJI CCNNGG 1 cut(s) 141
BsaWI WCCGGW 1 cut(s) 1237
Bsc4I CCNNNNNNNGG 2 cut(s) 527, 989
Bse1I ACTGG 5 cut(s) 292, 1117, 1165, 1354, 1402
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 5 cut(s) 333, 337, 362, 552, 1007
BseLI CCNNNNNNNGG 2 cut(s) 527, 989
BseMII CTCAG 5 cut(s) 337, 480, 1089, 1185, 1422
BseNI ACTGG 5 cut(s) 292, 1117, 1165, 1354, 1402
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 2 cut(s) 405, 499
BshNI GGYRCC 1 cut(s) 979
BsiHKCI CYCGRG 2 cut(s) 1286, 1334
BsiSI CCGG 1 cut(s) 1238
BslFI GGGAC 1 cut(s) 890
BslI CCNNNNNNNGG 2 cut(s) 527, 989
BsmFI GGGAC 1 cut(s) 890
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 2 cut(s) 405, 499
BsoBI CYCGRG 2 cut(s) 1286, 1334
Bsp1407I TGTACA 1 cut(s) 814
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 1 cut(s) 993
BspANI GGCC 2 cut(s) 405, 499
BspCNI CTCAG 5 cut(s) 336, 481, 1090, 1186, 1423
BspFNI CGCG 1 cut(s) 595
BspLI GGNNCC 3 cut(s) 498, 981, 1118
BspPI GGATC 8 cut(s) 483, 1082, 1130, 1178, 1223, 1319, 1367, 1415
BspT107I GGYRCC 1 cut(s) 979
BsrGI TGTACA 1 cut(s) 814
BsrI ACTGG 5 cut(s) 292, 1117, 1165, 1354, 1402
BssECI CCNNGG 1 cut(s) 141
BssNI GRCGYC 1 cut(s) 980
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 942
BstACI GRCGYC 1 cut(s) 980
BstAUI TGTACA 1 cut(s) 814
BstDEI CTNAG 5 cut(s) 323, 489, 1098, 1194, 1431
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 5 cut(s) 333, 337, 362, 552, 1007
BstFNI CGCG 1 cut(s) 595
BstH2I RGCGCY 2 cut(s) 57, 983
BstHHI GCGC 3 cut(s) 56, 242, 982
BstMWI GCNNNNNNNGC 5 cut(s) 130, 158, 297, 545, 888
BstNSI RCATGY 1 cut(s) 886
BstSFI CTRYAG 3 cut(s) 1206, 1251, 1299
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 1 cut(s) 1394
BsuRI GGCC 2 cut(s) 405, 499
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 5 cut(s) 333, 337, 362, 552, 1007
BtsIMutI CAGTG 3 cut(s) 485, 1347, 1395
CfoI GCGC 3 cut(s) 56, 242, 982
Cfr13I GGNCC 3 cut(s) 403, 497, 518
Csp6I GTAC 1 cut(s) 815
CviAII CATG 7 cut(s) 7, 142, 155, 212, 883, 929, 1012
CviQI GTAC 1 cut(s) 815
DdeI CTNAG 5 cut(s) 323, 489, 1098, 1194, 1431
DinI GGCGCC 1 cut(s) 981
DraI TTTAAA 1 cut(s) 726
DriI GACNNNNNGTC 1 cut(s) 883
Eam1105I GACNNNNNGTC 1 cut(s) 883
EciI GGCGGA 1 cut(s) 1008
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 518
Eco47III AGCGCT 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 462, 701
Eco88I CYCGRG 2 cut(s) 1286, 1334
EcoO109I RGGNCCY 1 cut(s) 497
EcoRI GAATTC 2 cut(s) 675, 755
EcoT14I CCWWGG 1 cut(s) 141
EgeI GGCGCC 1 cut(s) 981
EheI GGCGCC 1 cut(s) 981
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 7 cut(s) 10, 145, 158, 215, 886, 932, 1015
FalI AAGNNNNNCTT 2 cut(s) 523, 555
FaqI GGGAC 1 cut(s) 890
FatI CATG 7 cut(s) 6, 141, 154, 211, 882, 928, 1011
FbaI TGATCA 1 cut(s) 466
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 5 cut(s) 340, 344, 369, 539, 1014
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 3 cut(s) 368, 1145, 1458
GlaI GCGC 3 cut(s) 55, 241, 981
GluI GCNGC 1 cut(s) 159
HaeII RGCGCY 2 cut(s) 57, 983
HaeIII GGCC 2 cut(s) 405, 499
HapII CCGG 1 cut(s) 1238
HhaI GCGC 3 cut(s) 56, 242, 982
Hin1I GRCGYC 1 cut(s) 980
Hin1II CATG 7 cut(s) 10, 145, 158, 215, 886, 932, 1015
Hin6I GCGC 3 cut(s) 54, 240, 980
HinP1I GCGC 3 cut(s) 54, 240, 980
HindIII AAGCTT 2 cut(s) 315, 1444
HinfI GANTC 3 cut(s) 105, 590, 1039
HpaII CCGG 1 cut(s) 1238
Hpy166II GTNNAC 1 cut(s) 347
Hpy188III TCNNGA 5 cut(s) 325, 528, 609, 809, 914
Hpy8I GTNNAC 1 cut(s) 347
HpyCH4III ACNGT 1 cut(s) 942
HpyCH4V TGCA 4 cut(s) 6, 124, 458, 882
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 158, 297, 545, 888
HpyF3I CTNAG 5 cut(s) 323, 489, 1098, 1194, 1431
Hsp92I GRCGYC 1 cut(s) 980
Hsp92II CATG 7 cut(s) 10, 145, 158, 215, 886, 932, 1015
HspAI GCGC 3 cut(s) 54, 240, 980
KasI GGCGCC 1 cut(s) 979
Ksp22I TGATCA 1 cut(s) 466
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 2 cut(s) 139, 330
MaeI CTAG 3 cut(s) 368, 1145, 1458
MaeIII GTNAC 2 cut(s) 796, 1004
MboII GAAGA 3 cut(s) 92, 158, 1399
MfeI CAATTG 1 cut(s) 920
MluCI AATT 6 cut(s) 178, 232, 675, 755, 920, 1048
Mly113I GGCGCC 1 cut(s) 980
MmeI TCCRAC 1 cut(s) 685
MroXI GAANNNNTTC 1 cut(s) 675
MseI TTAA 4 cut(s) 267, 725, 1035, 1047
MslI CAYNNNNRTG 1 cut(s) 1016
MspI CCGG 1 cut(s) 1238
MssI GTTTAAAC 1 cut(s) 726
MunI CAATTG 1 cut(s) 920
Mva1269I GAATGC 1 cut(s) 458
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 5 cut(s) 130, 158, 297, 545, 888
NarI GGCGCC 1 cut(s) 980
NcoI CCATGG 1 cut(s) 141
NlaIII CATG 7 cut(s) 10, 145, 158, 215, 886, 932, 1015
NlaIV GGNNCC 3 cut(s) 498, 981, 1118
NmuCI GTSAC 1 cut(s) 1004
NspI RCATGY 1 cut(s) 886
PctI GAATGC 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 675
PfeI GAWTC 3 cut(s) 105, 590, 1039
PflMI CCANNNNNTGG 1 cut(s) 527
PkrI GCNGC 1 cut(s) 160
PluTI GGCGCC 1 cut(s) 983
PmeI GTTTAAAC 1 cut(s) 726
PspN4I GGNNCC 3 cut(s) 498, 981, 1118
PspPI GGNCC 3 cut(s) 403, 497, 518
RsaI GTAC 1 cut(s) 816
RsaNI GTAC 1 cut(s) 815
RseI CAYNNNNRTG 1 cut(s) 1016
SaqAI TTAA 4 cut(s) 267, 725, 1035, 1047
SatI GCNGC 1 cut(s) 159
Sau96I GGNCC 3 cut(s) 403, 497, 518
SfaNI GCATC 2 cut(s) 139, 330
SfcI CTRYAG 3 cut(s) 1206, 1251, 1299
SfoI GGCGCC 1 cut(s) 981
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 1016
SmlI CTYRAG 1 cut(s) 1382
SmoI CTYRAG 1 cut(s) 1382
Sse9I AATT 6 cut(s) 178, 232, 675, 755, 920, 1048
SsiI CCGC 1 cut(s) 993
SspDI GGCGCC 1 cut(s) 979
SspMI CTAG 3 cut(s) 368, 1145, 1458
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 1 cut(s) 942
TaqI TCGA 2 cut(s) 103, 174
TasI AATT 6 cut(s) 178, 232, 675, 755, 920, 1048
TatI WGTACW 1 cut(s) 814
TfiI GAWTC 3 cut(s) 105, 590, 1039
Tru1I TTAA 4 cut(s) 267, 725, 1035, 1047
Tru9I TTAA 4 cut(s) 267, 725, 1035, 1047
TscAI CASTG 3 cut(s) 485, 1354, 1402
TseFI GTSAC 1 cut(s) 1004
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 1004
TspDTI ATGAA 3 cut(s) 409, 945, 1404
TspGWI ACGGA 1 cut(s) 1212
TspRI CASTG 3 cut(s) 485, 1354, 1402
Van91I CCANNNNNTGG 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 518
XapI RAATTY 3 cut(s) 232, 675, 755
XceI RCATGY 1 cut(s) 886
XmnI GAANNNNTTC 1 cut(s) 675
XspI CTAG 3 cut(s) 368, 1145, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.