AT2G32235

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002328
Physical Location & Seq
Reverse (-)
13682557 .. 13684248
1692 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32235.1

Sequence Viewer

Length: 933 bp
ATGGCGACGCTAAACATGGCGGAAACCCTAGAAACCCTAATCGCTAATTCCCAAGAAGTTGATTACTCCTACGATCCCGAAGAAGATGACATTGATCTCTCCCTCCTCCGTCTCAACAGCTTCGGAAACTCCTCTGATCGCCGCCGTGCGAATTCCTCTCCTCCGCAATTCAAATCGTACGGATCTTTCGGTTCCTCTTCAACCACCGCAACAACCAGTCCCGTGAAACGTCCATCGCCGGAATCGAAGCAAGGTGACGAGCCGAGACGCAAGAAGCTGTTTATTCCGAGACCTGAGGAAGAAGAAGACCCGAATCTCATGGGATATTCAAAGATTCCGCTTCCTGTGGTTGAATTCAATCCGACGCAAATCCGTTCTCCTCTTTACAAGCGATCTTTATCCGATACATTTGCTTCGCCCGTTGGATCTACATTCGGGTCGGGTGGGTCGGGTTATACCCGTAACAGCGTCGCTCAAGAAACATCACCTCCTTCGGGTAATGTCCCTTCTCTCCCGCCACGTCCACGGATGTTCCGGAGGTCCGTCTCTGATCTTTCGCCGGCACCGTCCTCCAAGTCTCTTCTTGGATCTTCTCGCTCCAATGCAATTCCTGAAGGTGATCTCGCGAATCCAGAAAGCTCTGATGCCAATAAGATGCTGTATATTATCAAGGATGGAGTTCGTGAATTGGATCAATGGTGTAACAAGCTTCTTAAGTACGGTGAAGCAGTCTCCTCTGGCTCTGTGAAACAAGACGACAGTCCTAAGGCAGTAGATGAGGTAGTACAACAAGAGGAGCAACCAAAAGAGTGCAAAGAAGGTGTGAAGGTCAACAGACTCGGTGAGGCCTTTGTGGTTGAGATCAACTGTCCATGTGGAAGAAACTACCAAACTCTCTTCTCAGGCCGTGACTGCTACTACAAGCTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

34.11

Weight (kDa)

5.29

Isoelectric Point (pI)

72.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 562
AccII CGCG 1 cut(s) 626
AccIII TCCGGA 1 cut(s) 534
AciI CCGC 6 cut(s) 20, 142, 164, 207, 338, 515
AclWI GGATC 5 cut(s) 68, 190, 433, 595, 699
AcsI RAATTY 2 cut(s) 151, 353
AcuI CTGAAG 1 cut(s) 633
AfaI GTAC 3 cut(s) 179, 719, 786
AfiI CCNNNNNNNGG 1 cut(s) 494
AflII CTTAAG 1 cut(s) 713
AgsI TTSAA 5 cut(s) 172, 201, 330, 353, 358
AjiI CACGTC 1 cut(s) 521
AluBI AGCT 5 cut(s) 120, 277, 639, 709, 925
AluI AGCT 5 cut(s) 120, 277, 639, 709, 925
Alw26I GTCTC 6 cut(s) 116, 259, 283, 550, 582, 736
AlwI GGATC 5 cut(s) 68, 190, 433, 595, 699
Aor13HI TCCGGA 1 cut(s) 534
AoxI GGCC 2 cut(s) 846, 904
ApoI RAATTY 2 cut(s) 151, 353
AspS9I GGNCC 1 cut(s) 540
AsuHPI GGTGA 5 cut(s) 266, 477, 629, 734, 854
AvaII GGWCC 1 cut(s) 540
AxyI CCTNAGG 2 cut(s) 294, 765
BanI GGYRCC 1 cut(s) 562
BarI GAAGNNNNNNTAC 2 cut(s) 490, 522
BbsI GAAGAC 1 cut(s) 312
BccI CCATC 2 cut(s) 241, 668
BceAI ACGGC 2 cut(s) 129, 891
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BcoDI GTCTC 6 cut(s) 116, 259, 283, 550, 582, 736
BfaI CTAG 1 cut(s) 29
BfrI CTTAAG 1 cut(s) 713
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
Bme18I GGWCC 1 cut(s) 540
BmgBI CACGTC 1 cut(s) 521
BmgT120I GGNCC 1 cut(s) 540
BmiI GGNNCC 2 cut(s) 193, 564
BmsI GCATC 2 cut(s) 634, 645
BoxI GACNNNNGTC 1 cut(s) 759
BpiI GAAGAC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 397
BsaI GGTCTC 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 524
BsaWI WCCGGW 1 cut(s) 534
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 1 cut(s) 216
Bse21I CCTNAGG 2 cut(s) 294, 765
Bse8I GATNNNNATC 1 cut(s) 397
BseAI TCCGGA 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 524
BseGI GGATG 2 cut(s) 534, 679
BseJI GATNNNNATC 1 cut(s) 397
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMII CTCAG 2 cut(s) 285, 915
BseNI ACTGG 1 cut(s) 216
BseRI GAGGAG 6 cut(s) 95, 121, 150, 369, 724, 809
Bsh1236I CGCG 1 cut(s) 626
BshFI GGCC 2 cut(s) 848, 906
BshNI GGYRCC 1 cut(s) 562
BsiSI CCGG 3 cut(s) 239, 535, 560
BsiWI CGTACG 1 cut(s) 177
BslFI GGGAC 2 cut(s) 204, 488
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 6 cut(s) 116, 259, 283, 550, 582, 736
BsmBI CGTCTC 3 cut(s) 116, 259, 550
BsmFI GGGAC 2 cut(s) 204, 488
BsnI GGCC 2 cut(s) 848, 906
Bso31I GGTCTC 1 cut(s) 283
Bsp13I TCCGGA 1 cut(s) 534
Bsp68I TCGCGA 1 cut(s) 626
BspACI CCGC 6 cut(s) 20, 142, 164, 207, 338, 515
BspANI GGCC 2 cut(s) 848, 906
BspCNI CTCAG 2 cut(s) 286, 914
BspEI TCCGGA 1 cut(s) 534
BspFNI CGCG 1 cut(s) 626
BspLI GGNNCC 2 cut(s) 193, 564
BspPI GGATC 5 cut(s) 68, 190, 433, 595, 699
BspT107I GGYRCC 1 cut(s) 562
BspTI CTTAAG 1 cut(s) 713
BspTNI GGTCTC 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 559
BsrI ACTGG 1 cut(s) 216
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 1 cut(s) 524
Bst4CI ACNGT 4 cut(s) 567, 722, 761, 869
Bst6I CTCTTC 3 cut(s) 202, 585, 902
BstAFI CTTAAG 1 cut(s) 713
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 3 cut(s) 294, 765, 901
BstDSI CCRYGG 1 cut(s) 524
BstF5I GGATG 2 cut(s) 534, 679
BstFNI CGCG 1 cut(s) 626
BstMAI GTCTC 6 cut(s) 116, 259, 283, 550, 582, 736
BstMWI GCNNNNNNNGC 1 cut(s) 912
BstPAI GACNNNNGTC 1 cut(s) 759
BstUI CGCG 1 cut(s) 626
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 3 cut(s) 182, 425, 587
BstYI RGATCY 3 cut(s) 182, 425, 587
Bsu36I CCTNAGG 2 cut(s) 294, 765
BsuRI GGCC 2 cut(s) 848, 906
BtgI CCRYGG 1 cut(s) 524
BtgZI GCGATG 1 cut(s) 219
BtrI CACGTC 1 cut(s) 521
BtsCI GGATG 2 cut(s) 534, 679
BtuMI TCGCGA 1 cut(s) 626
Cac8I GCNNGC 1 cut(s) 561
Cfr10I RCCGGY 1 cut(s) 559
Cfr13I GGNCC 1 cut(s) 540
CseI GACGC 4 cut(s) 16, 276, 373, 457
Csp6I GTAC 3 cut(s) 178, 718, 785
CviAII CATG 3 cut(s) 16, 319, 873
CviJI RGCY 9 cut(s) 120, 262, 277, 639, 709, 741, 848, 906, 925
CviKI_1 RGCY 9 cut(s) 120, 262, 277, 639, 709, 741, 848, 906, 925
CviQI GTAC 3 cut(s) 178, 718, 785
DdeI CTNAG 3 cut(s) 294, 765, 901
Eam1104I CTCTTC 3 cut(s) 202, 585, 902
EarI CTCTTC 3 cut(s) 202, 585, 902
EciI GGCGGA 1 cut(s) 35
Eco147I AGGCCT 1 cut(s) 848
Eco31I GGTCTC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 540
Eco57I CTGAAG 1 cut(s) 633
Eco81I CCTNAGG 2 cut(s) 294, 765
EcoRI GAATTC 2 cut(s) 151, 353
Esp3I CGTCTC 3 cut(s) 116, 259, 550
FaeI CATG 3 cut(s) 19, 322, 876
FaiI YATR 5 cut(s) 17, 320, 456, 663, 874
FaqI GGGAC 2 cut(s) 204, 488
FatI CATG 3 cut(s) 15, 318, 872
FauI CCCGC 1 cut(s) 522
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 2 cut(s) 541, 686
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 1 cut(s) 29
GluI GCNGC 1 cut(s) 142
HaeIII GGCC 2 cut(s) 848, 906
HapII CCGG 3 cut(s) 239, 535, 560
HgaI GACGC 4 cut(s) 16, 276, 373, 457
Hin1II CATG 3 cut(s) 19, 322, 876
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 5 cut(s) 242, 313, 334, 628, 837
HpaII CCGG 3 cut(s) 239, 535, 560
HphI GGTGA 5 cut(s) 266, 477, 629, 734, 854
Hpy166II GTNNAC 2 cut(s) 524, 832
Hpy188I TCNGA 7 cut(s) 125, 136, 288, 363, 403, 550, 643
Hpy188III TCNNGA 7 cut(s) 77, 476, 535, 611, 625, 632, 683
Hpy8I GTNNAC 2 cut(s) 524, 832
Hpy99I CGWCG 3 cut(s) 10, 367, 473
HpyAV CCTTC 5 cut(s) 501, 516, 608, 812, 820
HpyCH4III ACNGT 4 cut(s) 567, 722, 761, 869
HpyCH4IV ACGT 2 cut(s) 229, 520
HpyCH4V TGCA 2 cut(s) 605, 813
HpyF10VI GCNNNNNNNGC 1 cut(s) 912
HpyF3I CTNAG 3 cut(s) 294, 765, 901
HpySE526I ACGT 2 cut(s) 229, 520
Hsp92II CATG 3 cut(s) 19, 322, 876
Kpn2I TCCGGA 1 cut(s) 534
KroI GCCGGC 1 cut(s) 559
KroNI GCCGGC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 602, 796
LweI GCATC 2 cut(s) 634, 645
MaeI CTAG 1 cut(s) 29
MaeII ACGT 2 cut(s) 229, 520
MaeIII GTNAC 4 cut(s) 254, 461, 701, 908
MflI RGATCY 3 cut(s) 182, 425, 587
MluCI AATT 6 cut(s) 46, 151, 167, 353, 606, 686
MlyI GAGTC 1 cut(s) 831
MmeI TCCRAC 2 cut(s) 386, 403
MroI TCCGGA 1 cut(s) 534
MroNI GCCGGC 1 cut(s) 559
MseI TTAA 1 cut(s) 714
MspCI CTTAAG 1 cut(s) 713
MspI CCGG 3 cut(s) 239, 535, 560
MvnI CGCG 1 cut(s) 626
MwoI GCNNNNNNNGC 1 cut(s) 912
NaeI GCCGGC 1 cut(s) 561
NgoMIV GCCGGC 1 cut(s) 559
NlaIII CATG 3 cut(s) 19, 322, 876
NlaIV GGNNCC 2 cut(s) 193, 564
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 2 cut(s) 254, 908
NruI TCGCGA 1 cut(s) 626
PceI AGGCCT 1 cut(s) 848
PcsI WCGNNNNNNNCGW 2 cut(s) 242, 563
PdiI GCCGGC 1 cut(s) 561
PfeI GAWTC 4 cut(s) 242, 313, 334, 628
Pfl23II CGTACG 1 cut(s) 177
PkrI GCNGC 1 cut(s) 143
PleI GAGTC 1 cut(s) 831
PpsI GAGTC 1 cut(s) 831
PshAI GACNNNNGTC 1 cut(s) 759
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 2 cut(s) 193, 564
PspPI GGNCC 1 cut(s) 540
PsuI RGATCY 3 cut(s) 182, 425, 587
RruI TCGCGA 1 cut(s) 626
RsaI GTAC 3 cut(s) 179, 719, 786
RsaNI GTAC 3 cut(s) 178, 718, 785
SaqAI TTAA 1 cut(s) 714
SatI GCNGC 1 cut(s) 142
Sau96I GGNCC 1 cut(s) 540
SchI GAGTC 1 cut(s) 831
SfaNI GCATC 2 cut(s) 634, 645
SinI GGWCC 1 cut(s) 540
SmlI CTYRAG 2 cut(s) 474, 713
SmoI CTYRAG 2 cut(s) 474, 713
Sse9I AATT 6 cut(s) 46, 151, 167, 353, 606, 686
SseBI AGGCCT 1 cut(s) 848
SsiI CCGC 6 cut(s) 20, 142, 164, 207, 338, 515
SspMI CTAG 1 cut(s) 29
StuI AGGCCT 1 cut(s) 848
TaaI ACNGT 4 cut(s) 567, 722, 761, 869
TaiI ACGT 2 cut(s) 232, 523
TaqI TCGA 1 cut(s) 245
TasI AATT 6 cut(s) 46, 151, 167, 353, 606, 686
TatI WGTACW 1 cut(s) 784
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 4 cut(s) 242, 313, 334, 628
Tru1I TTAA 1 cut(s) 714
Tru9I TTAA 1 cut(s) 714
TseFI GTSAC 2 cut(s) 254, 908
Tsp45I GTSAC 2 cut(s) 254, 908
TspGWI ACGGA 5 cut(s) 98, 195, 362, 532, 541
Vha464I CTTAAG 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 540
XapI RAATTY 2 cut(s) 151, 353
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.