Rmu_ssc0000204.1_g000047

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000204.1
OG0002328
Physical Location & Seq
Forward (+)
209432 .. 209986
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000204.1_g000047.1.cds

Sequence Viewer

Length: 555 bp
atggcagactcaaagacccaaccttccagcctcctcgacttcgccgtcaacaaccacttctccatgctccgcctcgactcctcctccaaaccccattcctccgccgccttgaaacgcccctctccgcctccctcccaccaacccaaccccaagaaagagaagctctctcctccccccctcttccccggcttttccaaaattacccctctccctcctctccccaaatcccactcgggtcgaacccgaacccgcaacccgctcctccgccgctgcatttccgaccccttcacccctcctcgccttgattctctctccgctcctcccgagaacgagaacgacgtcgttcccaattccgccgtcactgccatcacttccccgccgtccgtgatctccacgcgtccgccaccccgccccacgctccaccgcacagtctccgaccccacctcttcccccgccgccaaatccgcctcccgcgcttcctccagctccggcgacggcgacgacgatacccccaataccaaggtacgtaacactagttcaaaaattgaatcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

19.65

Weight (kDa)

10.22

Isoelectric Point (pI)

65.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AatII GACGTC 1 cut(s) 342
AccBSI CCGCTC 2 cut(s) 259, 317
AccII CGCG 2 cut(s) 397, 474
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 1 cut(s) 525
AflIII ACRYGT 1 cut(s) 395
AgsI TTSAA 3 cut(s) 112, 540, 548
AhlI ACTAGT 1 cut(s) 533
AluBI AGCT 2 cut(s) 163, 486
AluI AGCT 2 cut(s) 163, 486
Alw26I GTCTC 1 cut(s) 436
Ama87I CYCGRG 2 cut(s) 232, 323
ApeKI GCWGC 1 cut(s) 270
AspLEI GCGC 1 cut(s) 476
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 2 cut(s) 232, 323
BbvI GCAGC 1 cut(s) 257
BccI CCATC 1 cut(s) 374
BceAI ACGGC 4 cut(s) 29, 341, 364, 511
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 436
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 1 cut(s) 534
BisI GCNGC 4 cut(s) 105, 268, 271, 456
BlsI GCNGC 4 cut(s) 106, 269, 272, 457
Bme1390I CCNGG 1 cut(s) 186
BmeT110I CYCGRG 2 cut(s) 232, 323
BmrFI CCNGG 1 cut(s) 186
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 466
BpuMI CCSGG 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 527
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 184, 519
BsaXI ACNNNNNCTCC 4 cut(s) 44, 68, 74, 98
BseDI CCNNGG 2 cut(s) 184, 519
BseRI GAGGAG 8 cut(s) 23, 70, 73, 159, 204, 251, 285, 309
BseXI GCAGC 1 cut(s) 257
Bsh1236I CGCG 2 cut(s) 397, 474
BsiHKCI CYCGRG 2 cut(s) 232, 323
BsiSI CCGG 2 cut(s) 186, 489
BsmAI GTCTC 1 cut(s) 436
BsoBI CYCGRG 2 cut(s) 232, 323
Bsp143I GATC 1 cut(s) 387
BspFNI CGCG 2 cut(s) 397, 474
BsrBI CCGCTC 2 cut(s) 259, 317
BssECI CCNNGG 2 cut(s) 184, 519
BssMI GATC 1 cut(s) 387
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 519
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 2 cut(s) 185, 451
BstACI GRCGYC 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 527
BstFNI CGCG 2 cut(s) 397, 474
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 1 cut(s) 390
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 1 cut(s) 387
BstMWI GCNNNNNNNGC 3 cut(s) 362, 464, 473
BstSCI CCNGG 1 cut(s) 184
BstSNI TACGTA 1 cut(s) 527
BstUI CGCG 2 cut(s) 397, 474
BstV1I GCAGC 1 cut(s) 257
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 1 cut(s) 360
CfoI GCGC 1 cut(s) 476
CseI GACGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 524
CviAII CATG 1 cut(s) 64
CviJI RGCY 4 cut(s) 30, 163, 189, 486
CviKI_1 RGCY 4 cut(s) 30, 163, 189, 486
CviQI GTAC 1 cut(s) 524
DpnI GATC 1 cut(s) 389
DpnII GATC 1 cut(s) 387
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
Eam1104I CTCTTC 2 cut(s) 185, 451
EarI CTCTTC 2 cut(s) 185, 451
EciI GGCGGA 7 cut(s) 59, 91, 114, 254, 343, 390, 454
Eco105I TACGTA 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 519
Eco88I CYCGRG 2 cut(s) 232, 323
EcoT14I CCWWGG 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 1 cut(s) 67
FaiI YATR 1 cut(s) 65
FatI CATG 1 cut(s) 63
FauI CCCGC 6 cut(s) 257, 264, 384, 416, 460, 479
Fnu4HI GCNGC 4 cut(s) 105, 268, 271, 456
Fsp4HI GCNGC 4 cut(s) 105, 268, 271, 456
FspBI CTAG 1 cut(s) 534
GlaI GCGC 1 cut(s) 475
GluI GCNGC 4 cut(s) 105, 268, 271, 456
GsuI CTGGAG 1 cut(s) 466
HapII CCGG 2 cut(s) 186, 489
HgaI GACGC 1 cut(s) 386
HhaI GCGC 1 cut(s) 476
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 1 cut(s) 67
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 4 cut(s) 8, 77, 305, 548
HpaII CCGG 2 cut(s) 186, 489
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 2 cut(s) 280, 436
Hpy188III TCNNGA 2 cut(s) 323, 552
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 5 cut(s) 341, 344, 497, 503, 506
HpyAV CCTTC 2 cut(s) 33, 295
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 2 cut(s) 339, 526
HpyCH4V TGCA 1 cut(s) 273
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 464, 473
HpySE526I ACGT 2 cut(s) 339, 526
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 1 cut(s) 67
HspAI GCGC 1 cut(s) 474
Kzo9I GATC 1 cut(s) 387
LmnI GCTCC 5 cut(s) 72, 264, 322, 423, 491
LpnPI CCDG 4 cut(s) 40, 199, 496, 502
Lsp1109I GCAGC 1 cut(s) 257
MaeI CTAG 1 cut(s) 534
MaeII ACGT 2 cut(s) 339, 526
MaeIII GTNAC 2 cut(s) 358, 527
MalI GATC 1 cut(s) 389
MbiI CCGCTC 2 cut(s) 259, 317
MboI GATC 1 cut(s) 387
MboII GAAGA 2 cut(s) 172, 438
MluCI AATT 3 cut(s) 198, 349, 543
MluI ACGCGT 1 cut(s) 395
MlyI GAGTC 2 cut(s) 2, 71
MmeI TCCRAC 2 cut(s) 303, 459
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 2 cut(s) 186, 489
MspR9I CCNGG 1 cut(s) 186
MvnI CGCG 2 cut(s) 397, 474
MwoI GCNNNNNNNGC 3 cut(s) 362, 464, 473
NciI CCSGG 1 cut(s) 186
NdeII GATC 1 cut(s) 387
NlaIII CATG 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 358
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 336, 501
PfeI GAWTC 2 cut(s) 305, 548
PkrI GCNGC 4 cut(s) 106, 269, 272, 457
PleI GAGTC 2 cut(s) 2, 71
PpsI GAGTC 2 cut(s) 2, 71
Ppu21I YACGTR 1 cut(s) 527
PsrI GAACNNNNNNTAC 2 cut(s) 520, 552
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
SatI GCNGC 4 cut(s) 105, 268, 271, 456
Sau3AI GATC 1 cut(s) 387
SchI GAGTC 2 cut(s) 2, 71
ScrFI CCNGG 1 cut(s) 186
SetI ASST 7 cut(s) 25, 165, 342, 446, 488, 525, 529
SnaBI TACGTA 1 cut(s) 527
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 3 cut(s) 198, 349, 543
SspMI CTAG 1 cut(s) 534
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 2 cut(s) 342, 529
TaqI TCGA 3 cut(s) 36, 75, 238
TasI AATT 3 cut(s) 198, 349, 543
TauI GCSGC 3 cut(s) 107, 270, 458
TfiI GAWTC 2 cut(s) 305, 548
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 1 cut(s) 270
Tsp45I GTSAC 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 1 cut(s) 367
XspI CTAG 1 cut(s) 534
ZraI GACGTC 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.