AT3G13340

WD-40 repeat family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001622
Physical Location & Seq
Forward (+)
4331531 .. 4335092
3562 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G13340.1

Sequence Viewer

Length: 1344 bp
ATGTCTAATTATCGAGGAGATGATGCTGAGTACATGGAAGACGTAGATGATGAGATGGAAGATGTTGAAGATGATATGGATGAGGAGTTTCGTGGTGATGATCTTGGTGCTTCAGATTCTGACGTTGAAGAATTTGACTATTCGAACAATAAAGTAGCTGATACCACGGCTGAGCAAGCTCGGAAAGGGAAAGATATCCAGGGGATTCCGTGGGACAGACTTAATATTTCTAGAGAGAAATACAGACAAACTAGATTAGAACAGTATAAGAATTATGAAAATGTCCCTAATTCTGGAGAATCATCAGAAAAGGTTTGCAAGGTCACACAGAAAGGAGGACTATTTTATGACTTCTGGCGGAATTCACGATCTATTAAGTCTACTATACTACATTTCCAGCTGAGAAATTTGGTATGGGCAACTTCTAAGCACGACGTGTATCTTATGTCAAATTTTCTGGTTACACATTATTCTTCTCTAACGTCTGGAAAGCATGAAGTTCTCAATGTCCGTGGTCATGTTGCGCCATCTGAGAAACATCCTGGAAGTTTACTTGAAGGATTTACGCAGACTCAAGTAAGTACACTTGCAGTAAAAGATGACTTTCTGGTTGCTGGCGGGTTTCAAGGAGAACTTATTTGCAAGCATCTTGATAGACCGGGTGTCAGCTTTTGCTCTCGTATGACTTATGATGATAATGCTATAACCAATGCTATTGAGATCTACAACAAACCCAGTGGTGCACTTCATTTTACTGCGTCAAATAATGACTGTGGAGTTAGAGACTTTGACATGGAGAGATACCAGCTTGTTAAGCATTTCCGTTTTCCTTGGCCAGTGAATCACGCATCTTTGAGTCCTGATGGTAAATTACTGGCTATTGTTGGAGACAACCCGGAGGGCCTTATTGTAGACCCCAACACAGGAAAGACGTTGGAAACACTATCAGGACACTTGGACTTTTCCTTTGCCTCTGCGTGGCATCCGGATGGAGTCACTTTCTCTACAGGAAACCAAGATAAGACTTGTCGTGTATGGGACATCCGTAACCTATCTCAGTCTGTTACTGTCTTGAAGGGTAACCTGGGAGCAATCCGGTCAATCCGCTACACATCAGATGGAAAATACATGGCCATGGCTGAACCGGCTGACTTTGTCCATGTCTATGATGTCTCAAACGGGTATGAAACAGAGCAGGAAATCGACTTCTTTGGGGAAATCTCTGGAATATCTTTCAGCCCTGACACAGAGGCGCTATTTATTGGGGTATGGGACCGCACTTATGGTAGCCTCCTTGAGTTTGGTCGGCGCAGGAACTATTCCTACCTTGACTCGTATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

447

Amino Acids

50.62

Weight (kDa)

4.86

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 219 - 394 3.7e-15 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 244 - 331 1.2e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 245 - 427 1.3e-14 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 253 - 389 8.9e-08 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 275 - 425 3.5e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 280 - 426 2.4e-10 WDR3 first beta-propeller domain
WDR55 PF24796 282 - 393 5.7e-07 WDR55
WD40_CDC20-Fz PF24807 284 - 419 3.2e-10 CDC20/Fizzy WD40 domain
WD40 PF00400 309 - 347 1e-05 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 326 - 425 2.1e-12 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 326 - 426 2.6e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 380, 912
AccIII TCCGGA 1 cut(s) 985
AciI CCGC 4 cut(s) 358, 618, 1105, 1276
AcoI YGGCCR 2 cut(s) 833, 1131
AcsI RAATTY 4 cut(s) 131, 361, 406, 451
AcuI CTGAAG 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 436
AfaI GTAC 2 cut(s) 32, 583
AfiI CCNNNNNNNGG 3 cut(s) 293, 923, 978
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 5 cut(s) 68, 128, 557, 626, 1075
AhdI GACNNNNNGTC 1 cut(s) 662
AjiI CACGTC 1 cut(s) 436
AjnI CCWGG 3 cut(s) 198, 541, 1083
AluBI AGCT 5 cut(s) 158, 179, 400, 669, 808
AluI AGCT 5 cut(s) 158, 179, 400, 669, 808
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 3 cut(s) 777, 882, 1177
Alw44I GTGCAC 1 cut(s) 741
AlwNI CAGNNNCTG 1 cut(s) 119
Aor13HI TCCGGA 1 cut(s) 985
AoxI GGCC 3 cut(s) 833, 901, 1131
ApaLI GTGCAC 1 cut(s) 741
ApoI RAATTY 4 cut(s) 131, 361, 406, 451
ArsI GACNNNNNNTTYG 2 cut(s) 950, 982
AspLEI GCGC 3 cut(s) 526, 1255, 1311
AspS9I GGNCC 2 cut(s) 901, 1273
AsuC2I CCSGG 2 cut(s) 660, 896
AsuHPI GGTGA 1 cut(s) 107
AsuII TTCGAA 1 cut(s) 143
AvaII GGWCC 1 cut(s) 1273
BaeGI GKGCMC 1 cut(s) 745
BalI TGGCCA 2 cut(s) 835, 1133
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 745
BccI CCATC 5 cut(s) 49, 535, 857, 983, 1112
BceAI ACGGC 1 cut(s) 183
BciT130I CCWGG 3 cut(s) 200, 543, 1085
BcnI CCSGG 2 cut(s) 660, 896
BcoDI GTCTC 3 cut(s) 777, 882, 1177
BfaI CTAG 2 cut(s) 231, 252
BfmI CTRYAG 1 cut(s) 1005
BfoI RGCGCY 1 cut(s) 1256
BglII AGATCT 1 cut(s) 720
BlpI GCTNAGC 1 cut(s) 171
Bme1390I CCNGG 5 cut(s) 200, 543, 660, 896, 1085
Bme18I GGWCC 1 cut(s) 1273
BmeRI GACNNNNNGTC 1 cut(s) 662
BmgBI CACGTC 1 cut(s) 436
BmgT120I GGNCC 2 cut(s) 901, 1273
BmiI GGNNCC 1 cut(s) 1274
BmrFI CCNGG 5 cut(s) 200, 543, 660, 896, 1085
BmrI ACTGGG 1 cut(s) 729
BmsI GCATC 4 cut(s) 13, 655, 857, 991
BmuI ACTGGG 1 cut(s) 729
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 143
BpuEI CTTGAG 2 cut(s) 558, 1316
BpuMI CCSGG 2 cut(s) 660, 896
BsaJI CCNNGG 7 cut(s) 165, 199, 209, 511, 830, 1084, 1134
BsaWI WCCGGW 2 cut(s) 985, 1095
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 923, 978
Bse118I RCCGGY 1 cut(s) 1144
Bse1I ACTGG 3 cut(s) 735, 836, 879
BseAI TCCGGA 1 cut(s) 985
BseBI CCWGG 3 cut(s) 200, 543, 1085
BseDI CCNNGG 7 cut(s) 165, 199, 209, 511, 830, 1084, 1134
BseGI GGATG 5 cut(s) 85, 538, 982, 994, 1041
BseLI CCNNNNNNNGG 3 cut(s) 293, 923, 978
BseMII CTCAG 5 cut(s) 18, 162, 392, 522, 1070
BseNI ACTGG 3 cut(s) 735, 836, 879
BseRI GAGGAG 2 cut(s) 30, 98
BseSI GKGCMC 1 cut(s) 745
BshFI GGCC 3 cut(s) 835, 903, 1133
BsiHKAI GWGCWC 1 cut(s) 745
BsiSI CCGG 5 cut(s) 659, 896, 986, 1096, 1145
BslFI GGGAC 4 cut(s) 227, 269, 1052, 1286
BslI CCNNNNNNNGG 3 cut(s) 293, 923, 978
BsmAI GTCTC 3 cut(s) 777, 882, 1177
BsmFI GGGAC 4 cut(s) 227, 269, 1052, 1286
BsnI GGCC 3 cut(s) 835, 903, 1133
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 745
Bsp13I TCCGGA 1 cut(s) 985
Bsp143I GATC 3 cut(s) 100, 368, 720
Bsp1720I GCTNAGC 1 cut(s) 171
Bsp19I CCATGG 1 cut(s) 1134
BspACI CCGC 4 cut(s) 358, 618, 1105, 1276
BspANI GGCC 3 cut(s) 835, 903, 1133
BspCNI CTCAG 5 cut(s) 19, 163, 393, 523, 1069
BspEI TCCGGA 1 cut(s) 985
BspLI GGNNCC 1 cut(s) 1274
BspT104I TTCGAA 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 1144
BsrI ACTGG 3 cut(s) 735, 836, 879
BssAI RCCGGY 1 cut(s) 1144
BssECI CCNNGG 7 cut(s) 165, 199, 209, 511, 830, 1084, 1134
BssMI GATC 3 cut(s) 100, 368, 720
BssT1I CCWWGG 2 cut(s) 830, 1134
Bst2UI CCWGG 3 cut(s) 200, 543, 1085
Bst4CI ACNGT 3 cut(s) 264, 773, 1069
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 3 cut(s) 177, 616, 644
BstDEI CTNAG 6 cut(s) 27, 171, 401, 426, 531, 1056
BstDSI CCRYGG 4 cut(s) 165, 209, 511, 1134
BstEII GGTNACC 1 cut(s) 1079
BstF5I GGATG 5 cut(s) 85, 538, 982, 994, 1041
BstH2I RGCGCY 1 cut(s) 1256
BstHHI GCGC 3 cut(s) 526, 1255, 1311
BstKTI GATC 3 cut(s) 103, 371, 723
BstMAI GTCTC 3 cut(s) 777, 882, 1177
BstMBI GATC 3 cut(s) 100, 368, 720
BstMWI GCNNNNNNNGC 3 cut(s) 176, 814, 1145
BstNI CCWGG 3 cut(s) 200, 543, 1085
BstPI GGTNACC 1 cut(s) 1079
BstSCI CCNGG 5 cut(s) 198, 541, 658, 894, 1083
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 1 cut(s) 745
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 720
BstYI RGATCY 1 cut(s) 720
BsuRI GGCC 3 cut(s) 835, 903, 1133
BtgI CCRYGG 4 cut(s) 165, 209, 511, 1134
BtrI CACGTC 1 cut(s) 436
BtsCI GGATG 5 cut(s) 85, 538, 982, 994, 1041
BtsIMutI CAGTG 2 cut(s) 742, 843
Cac8I GCNNGC 3 cut(s) 177, 616, 644
CaiI CAGNNNCTG 1 cut(s) 119
CfoI GCGC 3 cut(s) 526, 1255, 1311
Cfr10I RCCGGY 1 cut(s) 1144
Cfr13I GGNCC 2 cut(s) 901, 1273
CseI GACGC 1 cut(s) 747
Csp6I GTAC 2 cut(s) 31, 582
CspCI CAANNNNNGTGG 4 cut(s) 493, 528, 718, 753
CviAII CATG 7 cut(s) 34, 494, 518, 793, 1129, 1135, 1160
CviQI GTAC 2 cut(s) 31, 582
DdeI CTNAG 6 cut(s) 27, 171, 401, 426, 531, 1056
DpnI GATC 3 cut(s) 102, 370, 722
DpnII GATC 3 cut(s) 100, 368, 720
DraIII CACNNNGTG 1 cut(s) 436
DriI GACNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 2 cut(s) 833, 1131
Eam1105I GACNNNNNGTC 1 cut(s) 662
EciI GGCGGA 1 cut(s) 373
Eco130I CCWWGG 2 cut(s) 830, 1134
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 1273
Eco57I CTGAAG 1 cut(s) 96
Eco91I GGTNACC 1 cut(s) 1079
EcoO109I RGGNCCY 1 cut(s) 901
EcoO65I GGTNACC 1 cut(s) 1079
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 198, 541, 1083
EcoRV GATATC 1 cut(s) 196
EcoT14I CCWWGG 2 cut(s) 830, 1134
ErhI CCWWGG 2 cut(s) 830, 1134
FaeI CATG 7 cut(s) 37, 497, 521, 796, 1132, 1138, 1163
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 4 cut(s) 227, 269, 1052, 1286
FatI CATG 7 cut(s) 33, 493, 517, 792, 1128, 1134, 1159
FauI CCCGC 1 cut(s) 611
FblI GTMKAC 2 cut(s) 380, 912
FokI GGATG 5 cut(s) 92, 525, 969, 1001, 1028
FspBI CTAG 2 cut(s) 231, 252
GlaI GCGC 3 cut(s) 525, 1254, 1310
GsuI CTGGAG 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 1256
HaeIII GGCC 3 cut(s) 835, 903, 1133
HapII CCGG 5 cut(s) 659, 896, 986, 1096, 1145
HgaI GACGC 1 cut(s) 747
HhaI GCGC 3 cut(s) 526, 1255, 1311
Hin1II CATG 7 cut(s) 37, 497, 521, 796, 1132, 1138, 1163
Hin6I GCGC 3 cut(s) 524, 1253, 1309
HinP1I GCGC 3 cut(s) 524, 1253, 1309
HinfI GANTC 8 cut(s) 116, 205, 299, 571, 841, 856, 993, 1331
HpaII CCGG 5 cut(s) 659, 896, 986, 1096, 1145
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 5 cut(s) 381, 551, 584, 743, 913
Hpy188I TCNGA 6 cut(s) 115, 121, 183, 307, 532, 1117
Hpy8I GTNNAC 5 cut(s) 381, 551, 584, 743, 913
Hpy99I CGWCG 1 cut(s) 437
HpyAV CCTTC 2 cut(s) 551, 1069
HpyCH4III ACNGT 3 cut(s) 264, 773, 1069
HpyCH4IV ACGT 5 cut(s) 42, 123, 435, 482, 932
HpyCH4V TGCA 4 cut(s) 318, 590, 642, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 814, 1145
HpyF3I CTNAG 6 cut(s) 27, 171, 401, 426, 531, 1056
HpySE526I ACGT 5 cut(s) 42, 123, 435, 482, 932
Hsp92II CATG 7 cut(s) 37, 497, 521, 796, 1132, 1138, 1163
HspAI GCGC 3 cut(s) 524, 1253, 1309
Kpn2I TCCGGA 1 cut(s) 985
Kzo9I GATC 3 cut(s) 100, 368, 720
LmnI GCTCC 1 cut(s) 1088
LweI GCATC 4 cut(s) 13, 655, 857, 991
MaeI CTAG 2 cut(s) 231, 252
MaeII ACGT 5 cut(s) 42, 123, 435, 482, 932
MaeIII GTNAC 6 cut(s) 322, 460, 994, 1046, 1063, 1079
MalI GATC 3 cut(s) 102, 370, 722
MboI GATC 3 cut(s) 100, 368, 720
MboII GAAGA 5 cut(s) 50, 71, 80, 140, 465
MflI RGATCY 1 cut(s) 720
MhlI GDGCHC 1 cut(s) 745
MlsI TGGCCA 2 cut(s) 835, 1133
MluCI AATT 8 cut(s) 7, 131, 271, 289, 361, 406, 451, 869
MluNI TGGCCA 2 cut(s) 835, 1133
MlyI GAGTC 4 cut(s) 565, 865, 1002, 1325
MmeI TCCRAC 2 cut(s) 865, 915
MnlI CCTC 7 cut(s) 8, 76, 329, 892, 982, 1243, 1301
Mox20I TGGCCA 2 cut(s) 835, 1133
MroI TCCGGA 1 cut(s) 985
MscI TGGCCA 2 cut(s) 835, 1133
MseI TTAA 4 cut(s) 222, 375, 813, 1342
MslI CAYNNNNRTG 3 cut(s) 987, 1133, 1164
Msp20I TGGCCA 2 cut(s) 835, 1133
MspA1I CMGCKG 1 cut(s) 400
MspI CCGG 5 cut(s) 659, 896, 986, 1096, 1145
MspR9I CCNGG 5 cut(s) 200, 543, 660, 896, 1085
MvaI CCWGG 3 cut(s) 200, 543, 1085
MwoI GCNNNNNNNGC 3 cut(s) 176, 814, 1145
NciI CCSGG 2 cut(s) 660, 896
NcoI CCATGG 1 cut(s) 1134
NdeII GATC 3 cut(s) 100, 368, 720
NlaIII CATG 7 cut(s) 37, 497, 521, 796, 1132, 1138, 1163
NlaIV GGNNCC 1 cut(s) 1274
NmuCI GTSAC 2 cut(s) 322, 994
NspV TTCGAA 1 cut(s) 143
PfeI GAWTC 4 cut(s) 116, 205, 299, 841
PflFI GACNNNGTC 1 cut(s) 1154
PfoI TCCNGGA 1 cut(s) 541
PleI GAGTC 4 cut(s) 565, 864, 1001, 1325
PpsI GAGTC 4 cut(s) 565, 864, 1001, 1325
Psp6I CCWGG 3 cut(s) 198, 541, 1083
PspEI GGTNACC 1 cut(s) 1079
PspGI CCWGG 3 cut(s) 198, 541, 1083
PspN4I GGNNCC 1 cut(s) 1274
PspPI GGNCC 2 cut(s) 901, 1273
PsrI GAACNNNNNNTAC 2 cut(s) 1307, 1339
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 720
PsyI GACNNNGTC 1 cut(s) 1154
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 2 cut(s) 32, 583
RsaNI GTAC 2 cut(s) 31, 582
RseI CAYNNNNRTG 3 cut(s) 987, 1133, 1164
SaqAI TTAA 4 cut(s) 222, 375, 813, 1342
Sau3AI GATC 3 cut(s) 100, 368, 720
Sau96I GGNCC 2 cut(s) 901, 1273
SchI GAGTC 4 cut(s) 565, 865, 1002, 1325
ScrFI CCNGG 5 cut(s) 200, 543, 660, 896, 1085
SduI GDGCHC 1 cut(s) 745
SfaNI GCATC 4 cut(s) 13, 655, 857, 991
SfcI CTRYAG 1 cut(s) 1005
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 1 cut(s) 1273
SmiMI CAYNNNNRTG 3 cut(s) 987, 1133, 1164
SmlI CTYRAG 2 cut(s) 573, 1295
SmoI CTYRAG 2 cut(s) 573, 1295
Sse9I AATT 8 cut(s) 7, 131, 271, 289, 361, 406, 451, 869
SsiI CCGC 4 cut(s) 358, 618, 1105, 1276
SspI AATATT 1 cut(s) 226
SspMI CTAG 2 cut(s) 231, 252
StyD4I CCNGG 5 cut(s) 198, 541, 658, 894, 1083
StyI CCWWGG 2 cut(s) 830, 1134
TaaI ACNGT 3 cut(s) 264, 773, 1069
TaiI ACGT 5 cut(s) 45, 126, 438, 485, 935
TaqI TCGA 3 cut(s) 13, 143, 1203
TasI AATT 8 cut(s) 7, 131, 271, 289, 361, 406, 451, 869
TatI WGTACW 2 cut(s) 30, 581
TfiI GAWTC 4 cut(s) 116, 205, 299, 841
Tru1I TTAA 4 cut(s) 222, 375, 813, 1342
Tru9I TTAA 4 cut(s) 222, 375, 813, 1342
TscAI CASTG 2 cut(s) 742, 843
TseFI GTSAC 2 cut(s) 322, 994
Tsp45I GTSAC 2 cut(s) 322, 994
TspDTI ATGAA 4 cut(s) 291, 510, 737, 1200
TspGWI ACGGA 4 cut(s) 198, 500, 812, 1034
TspRI CASTG 2 cut(s) 742, 843
Tth111I GACNNNGTC 1 cut(s) 1154
VneI GTGCAC 1 cut(s) 741
VpaK11BI GGWCC 1 cut(s) 1273
XapI RAATTY 4 cut(s) 131, 361, 406, 451
XbaI TCTAGA 1 cut(s) 230
XmiI GTMKAC 2 cut(s) 380, 912
XspI CTAG 2 cut(s) 231, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.