FvH4_3g41630

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001622
Physical Location & Seq
Reverse (-)
34940810 .. 34945029
4220 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41630.t1

Sequence Viewer

Length: 1329 bp
ATGTCCCACTACGCGGGTTACGTGGAAGACGAATATGAAATGGAGGATGTAGATGATGATATGGATGACGAATTTCGTGGAAGAGACCTGGCTGGCTCTGAGTCTGATGTCGATGAATTTGACTACGCGAATAGTAAAGTAGCTGATACAACTGCTGCTCAAGCAAGAAGAGGAAAAGACATCCAAGGCATCCCTTGGGATAGACTTAGCATTACTAGAGAGAAATACCGGCAAACTAGGTTAGAACAATACAAGAATTATGAAAACATTCCTAATTCCGGAGAAGGGTCTGGGAAGGATTGCGACATTACTAAGAAGGGATCTACATATTATGAGTTTAGACGTAACGCAAGATCAGTGAAATCAACTATTCTTCATTTTCAGTTGCGTAACTTGGTCTGGGCTACTTCAAAGCATGATGTCTACCTTATGTCACATTTTTCTGTCATTCATTGGTCTTCGATAAGTTGCAGCAAATCTGAGGTTCTAAATGTTTCAGGACATGTGGCACCATCAGAGAAACATCCCGGGAGTCTCTTGGAGGGATTTACACAGACTCAAGTGAGTACTCTTGCAGTAAAAGACAAATTGCTAGTTGCTGGAGGATTCCAGGGAGAACTTATTTGTAAGCATCTTGATCGGCCTGGAGTTAGCTTTTGTTCAAGGACAACATACGATGAAAATGCCATTACGAATGCTGTTGACATTTATGACAGTCCCAGCGGTGCAGTTCACTTTACAGCATCCAATAATGATTGTGGAGTCAGGGACTTCGACATGGAGAAATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGTTAATCATACTTCTTTGAGTCCCAATGGCAAACTTCTTGTGATTGTTGGTGACAATCCAGATGGGATGTTGGTGGATTCTCAGACTGGAAAGACTGTGAGGGCTTTATCTGGGCACTTGGATTTCTCGTTTGCATCAGCCTGGCATCCTGATGGTGTTACCTTTGCTACTGGGAACCAGGACAAAACTTGTCGGGTTTGGGATGTTCGAAACCTATCCAAGTCAGTTGCTGTTCTAAAGGGAAATCTTGGAGCTATTCGGTCCATTCGTTATACTTCTGATGGACGGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTATGAGAAGGAGCAGGAAATTGACTTCTTTGGTGAGATATCTGGTATATCATTCAGTCCAGACACGGAGTCGCTTTTCATTGGAGTGTGGGATCGTACGTATGGTAGTCTTCTCGAGTATGGACGACGCCGGGATTACTCTTATTTAGATTCTCTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

443

Amino Acids

49.77

Weight (kDa)

5.35

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 192 - 389 8.3e-16 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 239 - 414 3.5e-19 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 239 - 422 1.4e-15 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 240 - 391 2.8e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 243 - 348 3.3e-06 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 247 - 345 1.6e-07 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 256 - 386 5.9e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 275 - 421 7.8e-10 WDR3 first beta-propeller domain
WDR55 PF24796 276 - 389 1.6e-08 WDR55
WD40_CDC20-Fz PF24807 277 - 414 1.1e-08 CDC20/Fizzy WD40 domain
WD40 PF00400 304 - 342 4.2e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 321 - 420 6.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 423
AccII CGCG 2 cut(s) 14, 128
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 2 cut(s) 14, 723
AclWI GGATC 2 cut(s) 328, 1266
AcoI YGGCCR 1 cut(s) 818
AcsI RAATTY 3 cut(s) 71, 116, 785
AcyI GRCGYC 1 cut(s) 1294
AfaI GTAC 2 cut(s) 568, 1264
AfiI CCNNNNNNNGG 3 cut(s) 13, 278, 285
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 2 cut(s) 411, 663
AhdI GACNNNNNGTC 1 cut(s) 1234
AjnI CCWGG 5 cut(s) 87, 609, 643, 962, 999
AluBI AGCT 4 cut(s) 143, 654, 793, 1076
AluI AGCT 4 cut(s) 143, 654, 793, 1076
Alw26I GTCTC 2 cut(s) 78, 539
AlwI GGATC 2 cut(s) 328, 1266
AlwNI CAGNNNCTG 1 cut(s) 1052
Ama87I CYCGRG 2 cut(s) 527, 1280
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 2 cut(s) 641, 818
ApeKI GCWGC 2 cut(s) 155, 471
ApoI RAATTY 3 cut(s) 71, 116, 785
Asp700I GAANNNNTTC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 1083
AsuC2I CCSGG 3 cut(s) 528, 529, 1298
AsuHPI GGTGA 2 cut(s) 884, 1211
AsuII TTCGAA 1 cut(s) 1030
AvaI CYCGRG 2 cut(s) 527, 1280
AvaII GGWCC 1 cut(s) 1083
BaeGI GKGCMC 1 cut(s) 939
BalI TGGCCA 1 cut(s) 820
BanI GGYRCC 1 cut(s) 508
BbsI GAAGAC 3 cut(s) 33, 450, 1268
BbvI GCAGC 2 cut(s) 142, 483
BccI CCATC 5 cut(s) 520, 878, 968, 1097, 1114
BcgI CGANNNNNNTGC 4 cut(s) 620, 654, 665, 699
BciT130I CCWGG 5 cut(s) 89, 611, 645, 964, 1001
BcnI CCSGG 3 cut(s) 528, 529, 1298
BcoDI GTCTC 2 cut(s) 78, 539
BfaI CTAG 3 cut(s) 216, 237, 593
BisI GCNGC 2 cut(s) 156, 472
BlsI GCNGC 2 cut(s) 157, 473
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 8 cut(s) 89, 528, 529, 611, 645, 964, 1001, 1298
Bme18I GGWCC 1 cut(s) 1083
BmeRI GACNNNNNGTC 1 cut(s) 1234
BmeT110I CYCGRG 2 cut(s) 527, 1280
BmgT120I GGNCC 1 cut(s) 1083
BmiI GGNNCC 2 cut(s) 510, 998
BmrFI CCNGG 8 cut(s) 89, 528, 529, 611, 645, 964, 1001, 1298
BmrI ACTGGG 1 cut(s) 1002
BmsI GCATC 5 cut(s) 198, 640, 752, 965, 976
BmuI ACTGGG 1 cut(s) 1002
BpiI GAAGAC 3 cut(s) 33, 450, 1268
BpmI CTGGAG 2 cut(s) 621, 666
Bpu14I TTCGAA 1 cut(s) 1030
BpuEI CTTGAG 2 cut(s) 144, 543
BpuMI CCSGG 3 cut(s) 528, 529, 1298
BsaAI YACGTR 2 cut(s) 22, 1266
BsaHI GRCGYC 1 cut(s) 1294
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 5 cut(s) 184, 194, 527, 610, 815
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 278, 285
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 3 cut(s) 821, 913, 997
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 5 cut(s) 89, 611, 645, 964, 1001
BseDI CCNNGG 5 cut(s) 184, 194, 527, 610, 815
BseGI GGATG 9 cut(s) 52, 70, 180, 189, 523, 743, 894, 967, 1030
BseLI CCNNNNNNNGG 3 cut(s) 13, 278, 285
BseMII CTCAG 3 cut(s) 90, 471, 917
BseNI ACTGG 3 cut(s) 821, 913, 997
BseSI GKGCMC 1 cut(s) 939
BseXI GCAGC 2 cut(s) 142, 483
BseYI CCCAGC 1 cut(s) 719
BsgI GTGCAG 1 cut(s) 747
Bsh1236I CGCG 2 cut(s) 14, 128
BshFI GGCC 2 cut(s) 643, 820
BshNI GGYRCC 1 cut(s) 508
BsiHKCI CYCGRG 2 cut(s) 527, 1280
BsiSI CCGG 4 cut(s) 229, 279, 528, 1297
BsiWI CGTACG 1 cut(s) 1262
BslFI GGGAC 3 cut(s) 702, 782, 828
BslI CCNNNNNNNGG 3 cut(s) 13, 278, 285
BsmAI GTCTC 2 cut(s) 78, 539
BsmFI GGGAC 3 cut(s) 702, 782, 828
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 2 cut(s) 643, 820
Bso31I GGTCTC 1 cut(s) 78
BsoBI CYCGRG 2 cut(s) 527, 1280
Bsp119I TTCGAA 1 cut(s) 1030
Bsp1286I GDGCHC 1 cut(s) 939
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 4 cut(s) 320, 353, 637, 1258
BspACI CCGC 2 cut(s) 14, 723
BspANI GGCC 2 cut(s) 643, 820
BspCNI CTCAG 3 cut(s) 91, 472, 916
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 2 cut(s) 14, 128
BspLI GGNNCC 2 cut(s) 510, 998
BspPI GGATC 2 cut(s) 328, 1266
BspT104I TTCGAA 1 cut(s) 1030
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 3 cut(s) 821, 913, 997
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 5 cut(s) 184, 194, 527, 610, 815
BssMI GATC 4 cut(s) 320, 353, 637, 1258
BssNI GRCGYC 1 cut(s) 1294
BssT1I CCWWGG 3 cut(s) 184, 194, 815
Bst2UI CCWGG 5 cut(s) 89, 611, 645, 964, 1001
Bst4CI ACNGT 3 cut(s) 716, 919, 1110
Bst6I CTCTTC 2 cut(s) 76, 163
BstACI GRCGYC 1 cut(s) 1294
BstBAI YACGTR 2 cut(s) 22, 1266
BstBI TTCGAA 1 cut(s) 1030
BstC8I GCNNGC 2 cut(s) 94, 1131
BstDEI CTNAG 6 cut(s) 99, 206, 312, 480, 798, 903
BstF5I GGATG 9 cut(s) 52, 70, 180, 189, 523, 743, 894, 967, 1030
BstFNI CGCG 2 cut(s) 14, 128
BstKTI GATC 4 cut(s) 323, 356, 640, 1261
BstMAI GTCTC 2 cut(s) 78, 539
BstMBI GATC 4 cut(s) 320, 353, 637, 1258
BstMWI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
BstNI CCWGG 5 cut(s) 89, 611, 645, 964, 1001
BstNSI RCATGY 2 cut(s) 506, 1148
BstSCI CCNGG 8 cut(s) 87, 526, 527, 609, 643, 962, 999, 1296
BstSLI GKGCMC 1 cut(s) 939
BstSNI TACGTA 1 cut(s) 1266
BstUI CGCG 2 cut(s) 14, 128
BstV1I GCAGC 2 cut(s) 142, 483
BstV2I GAAGAC 3 cut(s) 33, 450, 1268
BstX2I RGATCY 1 cut(s) 320
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 2 cut(s) 643, 820
BtsCI GGATG 9 cut(s) 52, 70, 180, 189, 523, 743, 894, 967, 1030
BtsIMutI CAGTG 1 cut(s) 363
Cac8I GCNNGC 2 cut(s) 94, 1131
CaiI CAGNNNCTG 1 cut(s) 1052
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 1083
Cfr9I CCCGGG 1 cut(s) 527
CseI GACGC 1 cut(s) 1302
Csp6I GTAC 2 cut(s) 567, 1263
CviAII CATG 4 cut(s) 416, 503, 778, 1145
CviQI GTAC 2 cut(s) 567, 1263
DdeI CTNAG 6 cut(s) 99, 206, 312, 480, 798, 903
DpnI GATC 4 cut(s) 322, 355, 639, 1260
DpnII GATC 4 cut(s) 320, 353, 637, 1258
DriI GACNNNNNGTC 1 cut(s) 1234
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 2 cut(s) 76, 163
Eam1105I GACNNNNNGTC 1 cut(s) 1234
EarI CTCTTC 2 cut(s) 76, 163
Eco105I TACGTA 1 cut(s) 1266
Eco130I CCWWGG 3 cut(s) 184, 194, 815
Eco31I GGTCTC 1 cut(s) 78
Eco32I GATATC 1 cut(s) 1206
Eco47I GGWCC 1 cut(s) 1083
Eco88I CYCGRG 2 cut(s) 527, 1280
EcoRII CCWGG 5 cut(s) 87, 609, 643, 962, 999
EcoRV GATATC 1 cut(s) 1206
EcoT14I CCWWGG 3 cut(s) 184, 194, 815
ErhI CCWWGG 3 cut(s) 184, 194, 815
FaeI CATG 4 cut(s) 419, 506, 781, 1148
FaqI GGGAC 3 cut(s) 702, 782, 828
FatI CATG 4 cut(s) 415, 502, 777, 1144
FauI CCCGC 1 cut(s) 7
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 156, 472
FokI GGATG 9 cut(s) 59, 77, 167, 176, 510, 730, 901, 954, 1037
Fsp4HI GCNGC 2 cut(s) 156, 472
FspBI CTAG 3 cut(s) 216, 237, 593
GluI GCNGC 2 cut(s) 156, 472
GsaI CCCAGC 1 cut(s) 723
GsuI CTGGAG 2 cut(s) 621, 666
HaeIII GGCC 2 cut(s) 643, 820
HapII CCGG 4 cut(s) 229, 279, 528, 1297
HgaI GACGC 1 cut(s) 1302
Hin1I GRCGYC 1 cut(s) 1294
Hin1II CATG 4 cut(s) 419, 506, 781, 1148
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HinfI GANTC 9 cut(s) 101, 532, 556, 606, 762, 841, 899, 1235, 1316
HpaII CCGG 4 cut(s) 229, 279, 528, 1297
HphI GGTGA 2 cut(s) 884, 1211
Hpy166II GTNNAC 3 cut(s) 424, 703, 733
Hpy188I TCNGA 6 cut(s) 100, 106, 481, 517, 906, 1102
Hpy188III TCNNGA 7 cut(s) 279, 498, 635, 881, 971, 1226, 1280
Hpy8I GTNNAC 3 cut(s) 424, 703, 733
Hpy99I CGWCG 1 cut(s) 1296
HpyAV CCTTC 4 cut(s) 278, 289, 310, 1168
HpyCH4III ACNGT 3 cut(s) 716, 919, 1110
HpyCH4IV ACGT 3 cut(s) 21, 343, 1265
HpyCH4V TGCA 5 cut(s) 471, 575, 728, 956, 1144
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
HpyF3I CTNAG 6 cut(s) 99, 206, 312, 480, 798, 903
HpySE526I ACGT 3 cut(s) 21, 343, 1265
Hsp92I GRCGYC 1 cut(s) 1294
Hsp92II CATG 4 cut(s) 419, 506, 781, 1148
Kpn2I TCCGGA 1 cut(s) 278
Kzo9I GATC 4 cut(s) 320, 353, 637, 1258
LmnI GCTCC 2 cut(s) 1073, 1177
Lsp1109I GCAGC 2 cut(s) 142, 483
LweI GCATC 5 cut(s) 198, 640, 752, 965, 976
MaeI CTAG 3 cut(s) 216, 237, 593
MaeII ACGT 3 cut(s) 21, 343, 1265
MaeIII GTNAC 6 cut(s) 17, 344, 389, 432, 872, 979
MalI GATC 4 cut(s) 322, 355, 639, 1260
MboI GATC 4 cut(s) 320, 353, 637, 1258
MboII GAAGA 6 cut(s) 38, 93, 180, 365, 450, 1268
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 1 cut(s) 939
MlsI TGGCCA 1 cut(s) 820
MluCI AATT 7 cut(s) 71, 116, 256, 274, 587, 785, 1185
MluNI TGGCCA 1 cut(s) 820
MlyI GAGTC 6 cut(s) 110, 541, 550, 771, 850, 1244
MnlI CCTC 6 cut(s) 37, 164, 475, 535, 596, 915
Mox20I TGGCCA 1 cut(s) 820
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 267
MscI TGGCCA 1 cut(s) 820
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 3 cut(s) 972, 1149, 1250
Msp20I TGGCCA 1 cut(s) 820
MspA1I CMGCKG 1 cut(s) 723
MspI CCGG 4 cut(s) 229, 279, 528, 1297
MspR9I CCNGG 8 cut(s) 89, 528, 529, 611, 645, 964, 1001, 1298
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 5 cut(s) 89, 611, 645, 964, 1001
MvnI CGCG 2 cut(s) 14, 128
MwoI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
NciI CCSGG 3 cut(s) 528, 529, 1298
NdeII GATC 4 cut(s) 320, 353, 637, 1258
NlaIII CATG 4 cut(s) 419, 506, 781, 1148
NlaIV GGNNCC 2 cut(s) 510, 998
NmuCI GTSAC 2 cut(s) 432, 872
NspI RCATGY 2 cut(s) 506, 1148
NspV TTCGAA 1 cut(s) 1030
PaeR7I CTCGAG 1 cut(s) 1280
PciI ACATGT 1 cut(s) 502
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 27, 1087
PctI GAATGC 1 cut(s) 700
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 3 cut(s) 606, 899, 1316
Pfl23II CGTACG 1 cut(s) 1262
PkrI GCNGC 2 cut(s) 157, 473
PleI GAGTC 6 cut(s) 109, 540, 550, 770, 849, 1243
PpsI GAGTC 6 cut(s) 109, 540, 550, 770, 849, 1243
Ppu21I YACGTR 2 cut(s) 22, 1266
PscI ACATGT 1 cut(s) 502
Psp6I CCWGG 5 cut(s) 87, 609, 643, 962, 999
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 5 cut(s) 87, 609, 643, 962, 999
PspLI CGTACG 1 cut(s) 1262
PspN4I GGNNCC 2 cut(s) 510, 998
PspPI GGNCC 1 cut(s) 1083
PstNI CAGNNNCTG 1 cut(s) 1052
PsuI RGATCY 1 cut(s) 320
RsaI GTAC 2 cut(s) 568, 1264
RsaNI GTAC 2 cut(s) 567, 1263
RseI CAYNNNNRTG 3 cut(s) 972, 1149, 1250
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 2 cut(s) 156, 472
Sau3AI GATC 4 cut(s) 320, 353, 637, 1258
Sau96I GGNCC 1 cut(s) 1083
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 6 cut(s) 110, 541, 550, 771, 850, 1244
ScrFI CCNGG 8 cut(s) 89, 528, 529, 611, 645, 964, 1001, 1298
SduI GDGCHC 1 cut(s) 939
SfaNI GCATC 5 cut(s) 198, 640, 752, 965, 976
Sfr274I CTCGAG 1 cut(s) 1280
SfuI TTCGAA 1 cut(s) 1030
SinI GGWCC 1 cut(s) 1083
SlaI CTCGAG 1 cut(s) 1280
SmaI CCCGGG 1 cut(s) 529
SmiMI CAYNNNNRTG 3 cut(s) 972, 1149, 1250
SmlI CTYRAG 3 cut(s) 159, 558, 1280
SmoI CTYRAG 3 cut(s) 159, 558, 1280
SnaBI TACGTA 1 cut(s) 1266
Sse9I AATT 7 cut(s) 71, 116, 256, 274, 587, 785, 1185
SsiI CCGC 2 cut(s) 14, 723
SspMI CTAG 3 cut(s) 216, 237, 593
StyD4I CCNGG 8 cut(s) 87, 526, 527, 609, 643, 962, 999, 1296
StyI CCWWGG 3 cut(s) 184, 194, 815
TaaI ACNGT 3 cut(s) 716, 919, 1110
TaiI ACGT 3 cut(s) 24, 346, 1268
TaqI TCGA 5 cut(s) 111, 461, 774, 1030, 1281
TaqII GACCGA 1 cut(s) 1071
TasI AATT 7 cut(s) 71, 116, 256, 274, 587, 785, 1185
TatI WGTACW 1 cut(s) 566
TfiI GAWTC 3 cut(s) 606, 899, 1316
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 1 cut(s) 363
TseFI GTSAC 2 cut(s) 432, 872
TseI GCWGC 2 cut(s) 155, 471
Tsp45I GTSAC 2 cut(s) 432, 872
TspDTI ATGAA 7 cut(s) 51, 129, 276, 365, 440, 693, 1234
TspGWI ACGGA 2 cut(s) 797, 1247
TspMI CCCGGG 1 cut(s) 527
TspRI CASTG 1 cut(s) 363
VpaK11BI GGWCC 1 cut(s) 1083
XapI RAATTY 3 cut(s) 71, 116, 785
XceI RCATGY 2 cut(s) 506, 1148
XhoI CTCGAG 1 cut(s) 1280
XmaI CCCGGG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 3 cut(s) 216, 237, 593
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.