Rroxscaffold_1G00006860

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001622
Physical Location & Seq
Forward (+)
8857293 .. 8860985
3693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006860.1

Sequence Viewer

Length: 771 bp
ATGTCTAGTGTCTCTTCTATAATGAGGTTTGGATACGATGGTTGTGGCATTGTTGATGAAATGGAAGATGTTCATGATGATGTGGGTGGCGAACTTCGTGGTAGAGAATTGGCTAGCTTGGAGTCTGATGCTCATGAATCGGACTATTTGCGTAACTTGGTCTGGGCTACATCAAAGCATGATGTCTACCTTATGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCACCACATCCGAGGTTCTAAATGTTTCAGGACATGTCGCACCATCAGAGCATACTTCTCTGAGTCCCGATCGTAAACTTCTTGTGGTAGTTGGTGACAATCCGGATGGGATGTTGGTCGATGCTCAAACTGGAAAGGTGGCGCTGACGCCTTTATGCGGACACTTGGATTCCTCGTTTGCATCAGCTTGGCATCCTGATGGTGTCACCTTTGCTACTGGGAACCAGGACAAAACCTGTCGGGTTTGGGATATTCGAAACCTATCCAAGTCATCAGTTGCTGTTTTAAAGGGCAACCTTGGAGCTATTAGGTCAATCCGTTATAGTTCTGATGGTCAGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAGTGCGGGTACGAGAAGGAGCAGGAAATTGATTTCTTTGGTGCGATATCTGGCATATCATTCAGTCCAGACACAGAGTCGCTTTTCATTGGAGTACGGGATCTTGCGTATGGTGGTCTTCTCGAGTATGGAAGACAAGAGTGTGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

256

Amino Acids

27.97

Weight (kDa)

4.76

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 49 - 235 6.8e-12 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 54 - 207 2.9e-15 Prp19 WD40 domain
WDR55 PF24796 72 - 207 2.9e-07 WDR55
Beta-prop_WDR3_1st PF25173 80 - 240 2.5e-09 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 81 - 200 8.4e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 82 - 237 5.3e-12 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 127 - 237 8.5e-14 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 140 - 240 1.9e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 186
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 2 cut(s) 390, 624
AclWI GGATC 1 cut(s) 726
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 2 cut(s) 629, 714
AfiI CCNNNNNNNGG 1 cut(s) 389
AflIII ACRYGT 1 cut(s) 265
AhdI GACNNNNNGTC 1 cut(s) 694
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 3 cut(s) 117, 419, 536
AluI AGCT 3 cut(s) 117, 419, 536
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 1 cut(s) 726
AlwNI CAGNNNCTG 1 cut(s) 512
Ama87I CYCGRG 1 cut(s) 740
Aor13HI TCCGGA 1 cut(s) 334
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 338, 430
AsuII TTCGAA 1 cut(s) 487
AsuNHI GCTAGC 1 cut(s) 113
AvaI CYCGRG 1 cut(s) 740
BaeI ACNNNNGTAYC 2 cut(s) 25, 58
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BbsI GAAGAC 3 cut(s) 213, 728, 757
BccI CCATC 6 cut(s) 32, 283, 332, 425, 557, 574
BciT130I CCWGG 1 cut(s) 458
BciVI GTATCC 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 16
BfaI CTAG 2 cut(s) 6, 114
BfoI RGCGCY 1 cut(s) 377
BfuI GTATCC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 458
BmeRI GACNNNNNGTC 1 cut(s) 694
BmeT110I CYCGRG 1 cut(s) 740
BmiI GGNNCC 1 cut(s) 455
BmrFI CCNGG 1 cut(s) 458
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 4 cut(s) 118, 343, 422, 433
BmtI GCTAGC 1 cut(s) 117
BmuI ACTGGG 1 cut(s) 459
BpiI GAAGAC 3 cut(s) 213, 728, 757
Bpu14I TTCGAA 1 cut(s) 487
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 243, 529
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 2 cut(s) 367, 454
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 243, 529
BseGI GGATG 4 cut(s) 239, 343, 348, 424
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 367, 454
Bsh1285I CGRYCG 1 cut(s) 304
BsiEI CGRYCG 1 cut(s) 304
BsiHKCI CYCGRG 1 cut(s) 740
BsiSI CCGG 1 cut(s) 335
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 282
BsoBI CYCGRG 1 cut(s) 740
Bsp119I TTCGAA 1 cut(s) 487
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 2 cut(s) 301, 718
BspACI CCGC 2 cut(s) 390, 624
BspCNI CTCAG 1 cut(s) 285
BspEI TCCGGA 1 cut(s) 334
BspHI TCATGA 2 cut(s) 73, 133
BspLI GGNNCC 1 cut(s) 455
BspOI GCTAGC 1 cut(s) 117
BspPI GGATC 1 cut(s) 726
BspT104I TTCGAA 1 cut(s) 487
BsrI ACTGG 2 cut(s) 367, 454
BssECI CCNNGG 2 cut(s) 243, 529
BssMI GATC 2 cut(s) 301, 718
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 529
Bst2UI CCWGG 1 cut(s) 458
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 380
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 2 cut(s) 115, 591
BstDEI CTNAG 1 cut(s) 293
BstF5I GGATG 4 cut(s) 239, 343, 348, 424
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 2 cut(s) 304, 721
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 2 cut(s) 301, 718
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 1 cut(s) 590
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 2 cut(s) 269, 608
BstSCI CCNGG 1 cut(s) 456
BstV2I GAAGAC 3 cut(s) 213, 728, 757
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BsuI GTATCC 1 cut(s) 26
BtsCI GGATG 4 cut(s) 239, 343, 348, 424
Cac8I GCNNGC 2 cut(s) 115, 591
CaiI CAGNNNCTG 1 cut(s) 512
CciI TCATGA 2 cut(s) 73, 133
CfoI GCGC 1 cut(s) 376
CseI GACGC 1 cut(s) 388
Csp6I GTAC 2 cut(s) 628, 713
CviAII CATG 5 cut(s) 74, 134, 179, 266, 605
CviJI RGCY 6 cut(s) 113, 117, 167, 419, 536, 589
CviKI_1 RGCY 6 cut(s) 113, 117, 167, 419, 536, 589
CviQI GTAC 2 cut(s) 628, 713
DdeI CTNAG 1 cut(s) 293
DpnI GATC 2 cut(s) 303, 720
DpnII GATC 2 cut(s) 301, 718
DraI TTTAAA 1 cut(s) 519
DriI GACNNNNNGTC 1 cut(s) 694
Eam1104I CTCTTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 529
Eco32I GATATC 1 cut(s) 666
Eco88I CYCGRG 1 cut(s) 740
EcoRII CCWGG 1 cut(s) 456
EcoRV GATATC 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 529
FaeI CATG 5 cut(s) 77, 137, 182, 269, 608
FaqI GGGAC 1 cut(s) 282
FatI CATG 5 cut(s) 73, 133, 178, 265, 604
FauI CCCGC 1 cut(s) 617
FblI GTMKAC 1 cut(s) 186
FokI GGATG 4 cut(s) 226, 350, 355, 411
FspBI CTAG 2 cut(s) 6, 114
GlaI GCGC 1 cut(s) 375
HaeII RGCGCY 1 cut(s) 377
HapII CCGG 1 cut(s) 335
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 5 cut(s) 77, 137, 182, 269, 608
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HinfI GANTC 5 cut(s) 122, 137, 295, 401, 695
HpaII CCGG 1 cut(s) 335
HphI GGTGA 2 cut(s) 338, 430
Hpy166II GTNNAC 2 cut(s) 187, 308
Hpy188I TCNGA 6 cut(s) 127, 142, 244, 280, 294, 562
Hpy188III TCNNGA 8 cut(s) 74, 134, 261, 299, 335, 428, 686, 740
Hpy8I GTNNAC 2 cut(s) 187, 308
HpyAV CCTTC 1 cut(s) 628
HpyCH4V TGCA 3 cut(s) 234, 413, 604
HpyF10VI GCNNNNNNNGC 1 cut(s) 590
HpyF3I CTNAG 1 cut(s) 293
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 5 cut(s) 77, 137, 182, 269, 608
HspAI GCGC 1 cut(s) 374
Kpn2I TCCGGA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 301, 718
LmnI GCTCC 2 cut(s) 533, 637
LweI GCATC 4 cut(s) 118, 343, 422, 433
MaeI CTAG 2 cut(s) 6, 114
MaeIII GTNAC 4 cut(s) 152, 195, 326, 436
MalI GATC 2 cut(s) 303, 720
MboI GATC 2 cut(s) 301, 718
MboII GAAGA 5 cut(s) 6, 77, 213, 728, 762
MflI RGATCY 1 cut(s) 718
MluCI AATT 2 cut(s) 107, 645
MlyI GAGTC 3 cut(s) 131, 304, 704
MnlI CCTC 3 cut(s) 18, 238, 415
MroI TCCGGA 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 3 cut(s) 227, 518, 769
MslI CAYNNNNRTG 3 cut(s) 78, 429, 609
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 458
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 1 cut(s) 590
NdeII GATC 2 cut(s) 301, 718
NheI GCTAGC 1 cut(s) 113
NlaIII CATG 5 cut(s) 77, 137, 182, 269, 608
NlaIV GGNNCC 1 cut(s) 455
NmuCI GTSAC 3 cut(s) 195, 326, 436
NspI RCATGY 2 cut(s) 269, 608
NspV TTCGAA 1 cut(s) 487
PaeR7I CTCGAG 1 cut(s) 740
PagI TCATGA 2 cut(s) 73, 133
PciI ACATGT 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 137, 401
Ple19I CGATCG 1 cut(s) 304
PleI GAGTC 3 cut(s) 130, 303, 703
PpsI GAGTC 3 cut(s) 130, 303, 703
PscI ACATGT 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 1 cut(s) 455
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 1 cut(s) 718
PvuI CGATCG 1 cut(s) 304
RsaI GTAC 2 cut(s) 629, 714
RsaNI GTAC 2 cut(s) 628, 713
RseI CAYNNNNRTG 3 cut(s) 78, 429, 609
SaqAI TTAA 3 cut(s) 227, 518, 769
Sau3AI GATC 2 cut(s) 301, 718
SchI GAGTC 3 cut(s) 131, 304, 704
ScrFI CCNGG 1 cut(s) 458
SfaNI GCATC 4 cut(s) 118, 343, 422, 433
Sfr274I CTCGAG 1 cut(s) 740
SfuI TTCGAA 1 cut(s) 487
SlaI CTCGAG 1 cut(s) 740
SmiMI CAYNNNNRTG 3 cut(s) 78, 429, 609
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 2 cut(s) 107, 645
SsiI CCGC 2 cut(s) 390, 624
SspMI CTAG 2 cut(s) 6, 114
StyD4I CCNGG 1 cut(s) 456
StyI CCWWGG 1 cut(s) 529
TaqI TCGA 3 cut(s) 351, 487, 741
TasI AATT 2 cut(s) 107, 645
TfiI GAWTC 2 cut(s) 137, 401
Tru1I TTAA 3 cut(s) 227, 518, 769
Tru9I TTAA 3 cut(s) 227, 518, 769
TseFI GTSAC 3 cut(s) 195, 326, 436
Tsp45I GTSAC 3 cut(s) 195, 326, 436
TspDTI ATGAA 6 cut(s) 62, 72, 150, 203, 213, 694
TspGWI ACGGA 1 cut(s) 539
XceI RCATGY 2 cut(s) 269, 608
XhoI CTCGAG 1 cut(s) 740
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 2 cut(s) 6, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.