Rw5G045490

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001622
Physical Location & Seq
Reverse (-)
79502281 .. 79506043
3763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045490.1

Sequence Viewer

Length: 1224 bp
ATGGACAGCGAGGAGTTTCAGGATCATGTCGTCAAGCTTATGGAGGAAGTCGAAGGGCATTCCCGTTCAAAGGGAGAGAATAGAGGAGAAGACATCCAAGGTATTCCTTGGGATAGACTTAGCATTACTAGAGAGAAATACCGGCAAACTAGGTTAGAACAATACAAGAATTATGAAGACATCCCTAATTCCGGAGAAGGGACAGGGAAGGACTGCAACATTACAGAAAAAGGATCTTTATATTATGAGTTTCAATGCAACACAAGATCAGTGAAACCAACTATCCTTCATTTTCAGTTGCGTAACTTGGTCTGGGCTACATCAAAGCATGATGTCTACCTTCTGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCGAAGCATCTGAGGTTCTAAATGTTTCAGGACATGTCACACCATCAGAGAAACATCCTGGAAGTCTATTGGAGGGACATACACAGACTCAAGTTAGTAGTCTTGCAGTGAAAGATAAGTTGCTAGTTTCTGGAGGATTCCAAGGAGAACTTATTTGCAAGCATCTTGATCGACCTGGAGTTAGCTTTTGTTCCAGGACAACTGATGATGATAATGCCATTACAAATGCTATTGAGATTTATGACAATCCCAGTGGTGCAGCTCACTTTACAGCCTCAAGTAATGATTGTGGAGTCAGAGACTTTGACATGGAGACATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGTGAATCATACTTCTCTGAGTCCCGATGGTAAACTTCTTGTGATAGTTGGTGACAATCCAGATGGGATGTTGGTCGACTCTCAAACTGGAAAGACGGTGATGTCTTTATGTGGACACTTGGATTTCTCGTTTGCATCAGCGTGGCATCCTGATGGTGTCACCTTTGCTACTGGGAACCAGGATAAGACCTGTCGGGTTTGGGATGTTCGAAACCTATCCAAGTCAGTTGCTGTTTTAAAGGGAAACCTTGGAGCTATTCGGTCAATCCGTTATACTTCTGATGGTCAGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTACGAGAAGGAGCAGGAAATCGATTTGTTTGGAGAGATATCTGGCATATCATTCAGTCCAGACACAGAGTCGCTTTTCATTGGAGTATGGGATCGTACATATGGTAGTCTTCTCGAGTATGGAAGACAAAAGTGTGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

407

Amino Acids

45.55

Weight (kDa)

5.31

Isoelectric Point (pI)

26.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 156 - 360 5.6e-16 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 211 - 298 1.3e-06 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 214 - 318 3.2e-06 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 215 - 363 3.4e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 216 - 406 1.6e-16 WDR5 beta-propeller domain
WD40_Gbeta PF25391 219 - 356 9.7e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 219 - 315 2.1e-06 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 248 - 359 4.1e-08 WDR55
WD40_CDC20-Fz PF24807 249 - 385 5.7e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 250 - 392 5.7e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 274 - 391 4e-14 THOC3 beta-propeller domain
WD40 PF00400 275 - 313 1e-05 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 292 - 392 3.8e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 835
AccI GTMKAC 2 cut(s) 336, 810
AccIII TCCGGA 1 cut(s) 191
AclWI GGATC 3 cut(s) 30, 241, 1179
AcoI YGGCCR 1 cut(s) 731
AfaI GTAC 2 cut(s) 1082, 1177
AfiI CCNNNNNNNGG 3 cut(s) 70, 191, 198
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 2 cut(s) 69, 254
AhdI GACNNNNNGTC 1 cut(s) 1147
AjnI CCWGG 4 cut(s) 439, 556, 575, 912
AluBI AGCT 5 cut(s) 37, 567, 644, 706, 989
AluI AGCT 5 cut(s) 37, 567, 644, 706, 989
Alw26I GTCTC 2 cut(s) 675, 689
AlwI GGATC 3 cut(s) 30, 241, 1179
AlwNI CAGNNNCTG 1 cut(s) 965
Ama87I CYCGRG 1 cut(s) 1193
Aor13HI TCCGGA 1 cut(s) 191
AoxI GGCC 1 cut(s) 731
ApeKI GCWGC 1 cut(s) 641
AsuHPI GGTGA 3 cut(s) 797, 844, 886
AsuII TTCGAA 1 cut(s) 943
AvaI CYCGRG 1 cut(s) 1193
BalI TGGCCA 1 cut(s) 733
BbsI GAAGAC 5 cut(s) 96, 183, 363, 1181, 1210
BbvI GCAGC 1 cut(s) 653
BccI CCATC 6 cut(s) 433, 755, 791, 881, 1010, 1027
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 4 cut(s) 441, 558, 577, 914
BcoDI GTCTC 2 cut(s) 675, 689
BfaI CTAG 3 cut(s) 129, 150, 506
BisI GCNGC 1 cut(s) 642
BlsI GCNGC 1 cut(s) 643
Bme1390I CCNGG 4 cut(s) 441, 558, 577, 914
BmeRI GACNNNNNGTC 1 cut(s) 1147
BmeT110I CYCGRG 1 cut(s) 1193
BmiI GGNNCC 1 cut(s) 911
BmrFI CCNGG 4 cut(s) 441, 558, 577, 914
BmrI ACTGGG 2 cut(s) 627, 915
BmsI GCATC 4 cut(s) 398, 553, 878, 889
BmuI ACTGGG 2 cut(s) 627, 915
BpiI GAAGAC 5 cut(s) 96, 183, 363, 1181, 1210
BpmI CTGGAG 2 cut(s) 534, 579
Bpu14I TTCGAA 1 cut(s) 943
BpuEI CTTGAG 2 cut(s) 456, 643
Bsa29I ATCGAT 1 cut(s) 1101
BsaJI CCNNGG 5 cut(s) 97, 107, 523, 728, 982
BsaWI WCCGGW 1 cut(s) 191
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 191, 198
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 4 cut(s) 633, 734, 826, 910
BseAI TCCGGA 1 cut(s) 191
BseBI CCWGG 4 cut(s) 441, 558, 577, 914
BseCI ATCGAT 1 cut(s) 1101
BseDI CCNNGG 5 cut(s) 97, 107, 523, 728, 982
BseGI GGATG 6 cut(s) 93, 180, 436, 807, 880, 943
BseLI CCNNNNNNNGG 3 cut(s) 70, 191, 198
BseMII CTCAG 2 cut(s) 384, 743
BseNI ACTGG 4 cut(s) 633, 734, 826, 910
BseRI GAGGAG 2 cut(s) 26, 99
BseXI GCAGC 1 cut(s) 653
BsgI GTGCAG 1 cut(s) 660
BshFI GGCC 1 cut(s) 733
BshVI ATCGAT 1 cut(s) 1101
BsiHKCI CYCGRG 1 cut(s) 1193
BsiSI CCGG 2 cut(s) 142, 192
BslFI GGGAC 3 cut(s) 214, 471, 741
BslI CCNNNNNNNGG 3 cut(s) 70, 191, 198
BsmAI GTCTC 2 cut(s) 675, 689
BsmFI GGGAC 3 cut(s) 214, 471, 741
BsmI GAATGC 1 cut(s) 58
BsnI GGCC 1 cut(s) 733
BsoBI CYCGRG 1 cut(s) 1193
Bsp119I TTCGAA 1 cut(s) 943
Bsp13I TCCGGA 1 cut(s) 191
Bsp143I GATC 5 cut(s) 22, 233, 266, 550, 1171
BspANI GGCC 1 cut(s) 733
BspCNI CTCAG 2 cut(s) 385, 744
BspDI ATCGAT 1 cut(s) 1101
BspEI TCCGGA 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 911
BspPI GGATC 3 cut(s) 30, 241, 1179
BspT104I TTCGAA 1 cut(s) 943
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 4 cut(s) 633, 734, 826, 910
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 5 cut(s) 97, 107, 523, 728, 982
BssMI GATC 5 cut(s) 22, 233, 266, 550, 1171
BssT1I CCWWGG 5 cut(s) 97, 107, 523, 728, 982
Bst2UI CCWGG 4 cut(s) 441, 558, 577, 914
Bst4CI ACNGT 1 cut(s) 832
BstBI TTCGAA 1 cut(s) 943
BstC8I GCNNGC 2 cut(s) 542, 1044
BstDEI CTNAG 4 cut(s) 119, 393, 711, 752
BstF5I GGATG 6 cut(s) 93, 180, 436, 807, 880, 943
BstKTI GATC 5 cut(s) 25, 236, 269, 553, 1174
BstMAI GTCTC 2 cut(s) 675, 689
BstMBI GATC 5 cut(s) 22, 233, 266, 550, 1171
BstMWI GCNNNNNNNGC 2 cut(s) 712, 1043
BstNI CCWGG 4 cut(s) 441, 558, 577, 914
BstNSI RCATGY 2 cut(s) 419, 1061
BstSCI CCNGG 4 cut(s) 439, 556, 575, 912
BstV1I GCAGC 1 cut(s) 653
BstV2I GAAGAC 5 cut(s) 96, 183, 363, 1181, 1210
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
Bsu15I ATCGAT 1 cut(s) 1101
BsuRI GGCC 1 cut(s) 733
BsuTUI ATCGAT 1 cut(s) 1101
BtsCI GGATG 6 cut(s) 93, 180, 436, 807, 880, 943
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 4 cut(s) 276, 495, 640, 741
Cac8I GCNNGC 2 cut(s) 542, 1044
CaiI CAGNNNCTG 1 cut(s) 965
Cfr10I RCCGGY 1 cut(s) 141
ClaI ATCGAT 1 cut(s) 1101
Csp6I GTAC 2 cut(s) 1081, 1176
CspCI CAANNNNNGTGG 2 cut(s) 616, 651
CviAII CATG 5 cut(s) 26, 329, 416, 691, 1058
CviJI RGCY 9 cut(s) 37, 317, 567, 644, 656, 706, 733, 989, 1042
CviKI_1 RGCY 9 cut(s) 37, 317, 567, 644, 656, 706, 733, 989, 1042
CviQI GTAC 2 cut(s) 1081, 1176
DdeI CTNAG 4 cut(s) 119, 393, 711, 752
DpnI GATC 5 cut(s) 24, 235, 268, 552, 1173
DpnII GATC 5 cut(s) 22, 233, 266, 550, 1171
DraI TTTAAA 1 cut(s) 972
DrdI GACNNNNNNGTC 1 cut(s) 835
DriI GACNNNNNGTC 1 cut(s) 1147
DseDI GACNNNNNNGTC 1 cut(s) 835
EaeI YGGCCR 1 cut(s) 731
Eam1105I GACNNNNNGTC 1 cut(s) 1147
Eco130I CCWWGG 5 cut(s) 97, 107, 523, 728, 982
Eco32I GATATC 1 cut(s) 1119
Eco88I CYCGRG 1 cut(s) 1193
EcoRII CCWGG 4 cut(s) 439, 556, 575, 912
EcoRV GATATC 1 cut(s) 1119
EcoT14I CCWWGG 5 cut(s) 97, 107, 523, 728, 982
ErhI CCWWGG 5 cut(s) 97, 107, 523, 728, 982
FaeI CATG 5 cut(s) 29, 332, 419, 694, 1061
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 3 cut(s) 214, 471, 741
FatI CATG 5 cut(s) 25, 328, 415, 690, 1057
FauNDI CATATG 1 cut(s) 1180
FblI GTMKAC 2 cut(s) 336, 810
Fnu4HI GCNGC 1 cut(s) 642
FokI GGATG 6 cut(s) 80, 167, 423, 814, 867, 950
Fsp4HI GCNGC 1 cut(s) 642
FspBI CTAG 3 cut(s) 129, 150, 506
GluI GCNGC 1 cut(s) 642
GsuI CTGGAG 2 cut(s) 534, 579
HaeIII GGCC 1 cut(s) 733
HapII CCGG 2 cut(s) 142, 192
Hin1II CATG 5 cut(s) 29, 332, 419, 694, 1061
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 7 cut(s) 469, 519, 675, 739, 754, 812, 1148
HpaII CCGG 2 cut(s) 142, 192
HphI GGTGA 3 cut(s) 797, 844, 886
Hpy166II GTNNAC 4 cut(s) 337, 767, 811, 848
Hpy188I TCNGA 5 cut(s) 394, 430, 680, 753, 1015
Hpy8I GTNNAC 4 cut(s) 337, 767, 811, 848
HpyAV CCTTC 6 cut(s) 47, 191, 202, 296, 350, 1081
HpyCH4III ACNGT 1 cut(s) 832
HpyCH4V TGCA 7 cut(s) 216, 258, 488, 540, 641, 869, 1057
HpyF10VI GCNNNNNNNGC 2 cut(s) 712, 1043
HpyF3I CTNAG 4 cut(s) 119, 393, 711, 752
Hsp92II CATG 5 cut(s) 29, 332, 419, 694, 1061
Kpn2I TCCGGA 1 cut(s) 191
Kzo9I GATC 5 cut(s) 22, 233, 266, 550, 1171
LmnI GCTCC 2 cut(s) 986, 1090
Lsp1109I GCAGC 1 cut(s) 653
LweI GCATC 4 cut(s) 398, 553, 878, 889
MaeI CTAG 3 cut(s) 129, 150, 506
MaeIII GTNAC 5 cut(s) 302, 345, 418, 785, 892
MalI GATC 5 cut(s) 24, 235, 268, 552, 1173
MboI GATC 5 cut(s) 22, 233, 266, 550, 1171
MboII GAAGA 5 cut(s) 101, 188, 363, 1181, 1215
MflI RGATCY 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 733
MluCI AATT 2 cut(s) 169, 187
MluNI TGGCCA 1 cut(s) 733
MlyI GAGTC 5 cut(s) 463, 684, 763, 806, 1157
MnlI CCTC 7 cut(s) 4, 37, 77, 388, 448, 509, 667
Mox20I TGGCCA 1 cut(s) 733
MroI TCCGGA 1 cut(s) 191
MscI TGGCCA 1 cut(s) 733
MseI TTAA 3 cut(s) 377, 971, 1222
MslI CAYNNNNRTG 3 cut(s) 874, 885, 1062
Msp20I TGGCCA 1 cut(s) 733
MspI CCGG 2 cut(s) 142, 192
MspR9I CCNGG 4 cut(s) 441, 558, 577, 914
Mva1269I GAATGC 1 cut(s) 58
MvaI CCWGG 4 cut(s) 441, 558, 577, 914
MwoI GCNNNNNNNGC 2 cut(s) 712, 1043
NdeI CATATG 1 cut(s) 1180
NdeII GATC 5 cut(s) 22, 233, 266, 550, 1171
NlaIII CATG 5 cut(s) 29, 332, 419, 694, 1061
NlaIV GGNNCC 1 cut(s) 911
NmuCI GTSAC 4 cut(s) 345, 418, 785, 892
NspI RCATGY 2 cut(s) 419, 1061
NspV TTCGAA 1 cut(s) 943
PaeR7I CTCGAG 1 cut(s) 1193
PciI ACATGT 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 1000
PctI GAATGC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 519, 739
PfoI TCCNGGA 2 cut(s) 439, 575
PkrI GCNGC 1 cut(s) 643
PleI GAGTC 5 cut(s) 463, 683, 762, 806, 1156
PpsI GAGTC 5 cut(s) 463, 683, 762, 806, 1156
PscI ACATGT 1 cut(s) 415
Psp6I CCWGG 4 cut(s) 439, 556, 575, 912
PspGI CCWGG 4 cut(s) 439, 556, 575, 912
PspN4I GGNNCC 1 cut(s) 911
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 1 cut(s) 233
RsaI GTAC 2 cut(s) 1082, 1177
RsaNI GTAC 2 cut(s) 1081, 1176
RseI CAYNNNNRTG 3 cut(s) 874, 885, 1062
SalI GTCGAC 1 cut(s) 809
SaqAI TTAA 3 cut(s) 377, 971, 1222
SatI GCNGC 1 cut(s) 642
Sau3AI GATC 5 cut(s) 22, 233, 266, 550, 1171
SchI GAGTC 5 cut(s) 463, 684, 763, 806, 1157
ScrFI CCNGG 4 cut(s) 441, 558, 577, 914
SfaNI GCATC 4 cut(s) 398, 553, 878, 889
Sfr274I CTCGAG 1 cut(s) 1193
SfuI TTCGAA 1 cut(s) 943
SlaI CTCGAG 1 cut(s) 1193
SmiMI CAYNNNNRTG 3 cut(s) 874, 885, 1062
SmlI CTYRAG 3 cut(s) 471, 658, 1193
SmoI CTYRAG 3 cut(s) 471, 658, 1193
Sse9I AATT 2 cut(s) 169, 187
SspMI CTAG 3 cut(s) 129, 150, 506
StyD4I CCNGG 4 cut(s) 439, 556, 575, 912
StyI CCWWGG 5 cut(s) 97, 107, 523, 728, 982
TaaI ACNGT 1 cut(s) 832
TaqI TCGA 6 cut(s) 51, 553, 810, 943, 1101, 1194
TaqII GACCGA 1 cut(s) 984
TasI AATT 2 cut(s) 169, 187
TfiI GAWTC 2 cut(s) 519, 739
Tru1I TTAA 3 cut(s) 377, 971, 1222
Tru9I TTAA 3 cut(s) 377, 971, 1222
TscAI CASTG 4 cut(s) 276, 495, 640, 741
TseFI GTSAC 4 cut(s) 345, 418, 785, 892
TseI GCWGC 1 cut(s) 641
Tsp45I GTSAC 4 cut(s) 345, 418, 785, 892
TspDTI ATGAA 5 cut(s) 189, 278, 353, 363, 1147
TspGWI ACGGA 2 cut(s) 710, 992
TspRI CASTG 4 cut(s) 276, 495, 640, 741
XceI RCATGY 2 cut(s) 419, 1061
XhoI CTCGAG 1 cut(s) 1193
XmiI GTMKAC 2 cut(s) 336, 810
XspI CTAG 3 cut(s) 129, 150, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.