MD11G1044000.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001622
Physical Location & Seq
Forward (+)
3886116 .. 3888897
2782 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1044000.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGTCCCATTACGCTGGTTATGTGGAGGATGAATACGAAATGGAAGATGTAGACGATGATATGGATGATGAATTTCGCGGTAGAGAAATGGGCGGCTCAGATTCTGATGTTGATGAATATGACTACTCGAATAGTAAAGTAGCTGATACAACTGCTGCTCAAGCAGGAAAAGGAAAGAAGACATCCAAGGCATTCCTTGGGATAGACTTAGCATTACTAGAGAGAAATAGCGACATACTAGGTTTGAACAATACAAGAATTATGAGAGTATTCCTAATTCTGGAGAAGGATCAGGGAAGACGAAGTACTAGATCAGTGAAATCAACCATTCTTCATTTTCAGTTGCGTAACCTGGTCTGGGCTACTTCCAAGCATGATGTCTACCTGATGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCACAAAGTCTGAGGTTCTCAATGTTTCTGGACATGTGGCACCATCGGAGAAACATTCTGGAAGTCTCTTGGAGGGATTTACGAGGACTCAAGTTAGTACTCTTGCTGTAAAAGATAAGTTGCTAGTTGCTGGAGGATTCCAGGGTGAACTTATTTGCAAGCATCTTGATCGGCCTGGAGTCAGCTTTTGTTCAAGGACAACTAATGCAATAACAAATGCTGTTGAGATTTATGATAGTCCTAGCGGGGCAGTTCACTTTACAGCCTCTAATAATGACTGTGGAGTCAGAGACTTTGACGTGGAGAAATTTCAGCTTACTAAGCATTTCCGTTATCCTTGGCCTGTGAATCATACTTCTCTGAGTCCTGATGGTAAACTTCTTGTGATTGTTGGAGACAATCCGGAAGGGATGTTGGTAAACTCTCAAACTGGAAAGACGGTGATGTCTTTAAGTGGGCACTTGGATTTCTCCTTCGCATCAGCATGGCATCCTGATGGTATGACCTTTGCTACTGGGAACCAGGACAAAACCTGCCGGATTTGGGATGTTCGAAACCTTTCCAAGTCAGTTGCTGTTTTGAAGGGGAACCTTGGAGCTATTCGGTCAATCCGGTATACTTCCGATGGTCAGTATATGATGATGGCAGAGCCAGCGGATTTTGTGCATGTCTATGATGTGAAAAGTGGGTATGAAAAGGAACAGGAGATTGATTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCGGACACAGAGTCGCTTTTTATTGGAGTGTGGGATCGTACATACGGTAGTCTTCTTGAGTATGGCCGAAGCCGGAACTACTCTTACCCTGGTGTTAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

426

Amino Acids

47.36

Weight (kDa)

5.62

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 181 - 372 4.9e-16 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 215 - 313 2.5e-07 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 221 - 330 6.5e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 222 - 405 2.8e-16 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 223 - 375 2.1e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 232 - 369 7.5e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 258 - 404 5.2e-11 WDR3 first beta-propeller domain
WDR55 PF24796 259 - 372 7.9e-08 WDR55
WD40_CDC20-Fz PF24807 261 - 397 4.5e-08 CDC20/Fizzy WD40 domain
WD40 PF00400 287 - 325 1e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 292 - 404 1.3e-14 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 304 - 404 2.5e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 710, 871
Acc36I ACCTGC 1 cut(s) 968
AccB1I GGYRCC 1 cut(s) 466
AccI GTMKAC 3 cut(s) 51, 381, 1043
AccII CGCG 1 cut(s) 78
AccIII TCCGGA 2 cut(s) 829, 1174
AciI CCGC 4 cut(s) 78, 93, 672, 1082
AclWI GGATC 2 cut(s) 297, 1215
AcoI YGGCCR 1 cut(s) 1237
AcsI RAATTY 2 cut(s) 71, 734
AfaI GTAC 3 cut(s) 307, 526, 1213
AfiI CCNNNNNNNGG 3 cut(s) 280, 358, 970
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 3 cut(s) 247, 621, 1009
AhdI GACNNNNNGTC 1 cut(s) 1183
AjiI CACGTC 1 cut(s) 727
AjnI CCWGG 5 cut(s) 351, 567, 601, 948, 1261
AluBI AGCT 4 cut(s) 143, 612, 742, 1025
AluI AGCT 4 cut(s) 143, 612, 742, 1025
Alw26I GTCTC 3 cut(s) 497, 711, 816
AlwI GGATC 2 cut(s) 297, 1215
AlwNI CAGNNNCTG 2 cut(s) 104, 1001
Aor13HI TCCGGA 2 cut(s) 829, 1174
AoxI GGCC 3 cut(s) 599, 767, 1237
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 2 cut(s) 71, 734
Asp700I GAANNNNTTC 1 cut(s) 985
AsuHPI GGTGA 3 cut(s) 584, 880, 1160
AsuII TTCGAA 1 cut(s) 979
BaeGI GKGCMC 1 cut(s) 888
BanI GGYRCC 1 cut(s) 466
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
BbsI GAAGAC 4 cut(s) 185, 304, 408, 1217
BbvI GCAGC 1 cut(s) 142
BccI CCATC 5 cut(s) 478, 791, 917, 1046, 1063
BcgI CGANNNNNNTGC 2 cut(s) 578, 612
BciT130I CCWGG 5 cut(s) 353, 569, 603, 950, 1263
BcoDI GTCTC 3 cut(s) 497, 711, 816
BfaI CTAG 5 cut(s) 218, 239, 309, 551, 669
BfuAI ACCTGC 1 cut(s) 968
BisI GCNGC 2 cut(s) 94, 156
BlsI GCNGC 2 cut(s) 95, 157
BmcAI AGTACT 2 cut(s) 307, 526
Bme1390I CCNGG 5 cut(s) 353, 569, 603, 950, 1263
BmeRI GACNNNNNGTC 1 cut(s) 1183
BmgBI CACGTC 1 cut(s) 727
BmiI GGNNCC 3 cut(s) 468, 947, 1016
BmrFI CCNGG 5 cut(s) 353, 569, 603, 950, 1263
BmrI ACTGGG 1 cut(s) 951
BmsI GCATC 3 cut(s) 598, 914, 925
BmuI ACTGGG 1 cut(s) 951
BpiI GAAGAC 4 cut(s) 185, 304, 408, 1217
BpmI CTGGAG 3 cut(s) 302, 579, 624
Bpu14I TTCGAA 1 cut(s) 979
BpuEI CTTGAG 3 cut(s) 144, 501, 1250
BsaJI CCNNGG 6 cut(s) 186, 196, 568, 764, 1018, 1261
BsaWI WCCGGW 3 cut(s) 829, 1038, 1174
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 358, 970
Bse1I ACTGG 2 cut(s) 862, 946
BseAI TCCGGA 2 cut(s) 829, 1174
BseBI CCWGG 5 cut(s) 353, 569, 603, 950, 1263
BseDI CCNNGG 6 cut(s) 186, 196, 568, 764, 1018, 1261
BseGI GGATG 6 cut(s) 34, 70, 182, 843, 916, 979
BseLI CCNNNNNNNGG 3 cut(s) 280, 358, 970
BseMII CTCAG 3 cut(s) 111, 429, 779
BseNI ACTGG 2 cut(s) 862, 946
BseSI GKGCMC 1 cut(s) 888
BseXI GCAGC 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 3 cut(s) 601, 769, 1239
BshNI GGYRCC 1 cut(s) 466
BsiSI CCGG 5 cut(s) 830, 964, 1039, 1175, 1246
BslI CCNNNNNNNGG 3 cut(s) 280, 358, 970
BsmAI GTCTC 3 cut(s) 497, 711, 816
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 3 cut(s) 601, 769, 1239
Bsp119I TTCGAA 1 cut(s) 979
Bsp1286I GDGCHC 1 cut(s) 888
Bsp13I TCCGGA 2 cut(s) 829, 1174
Bsp143I GATC 4 cut(s) 289, 311, 595, 1207
BspACI CCGC 4 cut(s) 78, 93, 672, 1082
BspANI GGCC 3 cut(s) 601, 769, 1239
BspCNI CTCAG 3 cut(s) 110, 430, 780
BspEI TCCGGA 2 cut(s) 829, 1174
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 3 cut(s) 468, 947, 1016
BspMI ACCTGC 1 cut(s) 968
BspPI GGATC 2 cut(s) 297, 1215
BspT104I TTCGAA 1 cut(s) 979
BspT107I GGYRCC 1 cut(s) 466
BsrI ACTGG 2 cut(s) 862, 946
BssECI CCNNGG 6 cut(s) 186, 196, 568, 764, 1018, 1261
BssMI GATC 4 cut(s) 289, 311, 595, 1207
BssNAI GTATAC 1 cut(s) 1044
BssT1I CCWWGG 4 cut(s) 186, 196, 764, 1018
Bst1107I GTATAC 1 cut(s) 1044
Bst2UI CCWGG 5 cut(s) 353, 569, 603, 950, 1263
Bst4CI ACNGT 3 cut(s) 707, 868, 1220
BstBI TTCGAA 1 cut(s) 979
BstC8I GCNNGC 2 cut(s) 587, 1080
BstDEI CTNAG 5 cut(s) 97, 208, 438, 747, 788
BstF5I GGATG 6 cut(s) 34, 70, 182, 843, 916, 979
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 4 cut(s) 292, 314, 598, 1210
BstMAI GTCTC 3 cut(s) 497, 711, 816
BstMBI GATC 4 cut(s) 289, 311, 595, 1207
BstMWI GCNNNNNNNGC 3 cut(s) 161, 748, 1079
BstNI CCWGG 5 cut(s) 353, 569, 603, 950, 1263
BstNSI RCATGY 2 cut(s) 464, 1097
BstSCI CCNGG 5 cut(s) 351, 567, 601, 948, 1261
BstSLI GKGCMC 1 cut(s) 888
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 4 cut(s) 185, 304, 408, 1217
BstXI CCANNNNNNTGG 1 cut(s) 14
BstZ17I GTATAC 1 cut(s) 1044
BsuRI GGCC 3 cut(s) 601, 769, 1239
BtrI CACGTC 1 cut(s) 727
BtsCI GGATG 6 cut(s) 34, 70, 182, 843, 916, 979
BtsIMutI CAGTG 1 cut(s) 321
BveI ACCTGC 1 cut(s) 968
Cac8I GCNNGC 2 cut(s) 587, 1080
CaiI CAGNNNCTG 2 cut(s) 104, 1001
CsiI ACCWGGT 1 cut(s) 351
Csp6I GTAC 3 cut(s) 306, 525, 1212
CviAII CATG 4 cut(s) 374, 461, 912, 1094
CviQI GTAC 3 cut(s) 306, 525, 1212
DdeI CTNAG 5 cut(s) 97, 208, 438, 747, 788
DpnI GATC 4 cut(s) 291, 313, 597, 1209
DpnII GATC 4 cut(s) 289, 311, 595, 1207
DrdI GACNNNNNNGTC 2 cut(s) 710, 871
DriI GACNNNNNGTC 1 cut(s) 1183
DseDI GACNNNNNNGTC 2 cut(s) 710, 871
EaeI YGGCCR 1 cut(s) 1237
Eam1105I GACNNNNNGTC 1 cut(s) 1183
Eco130I CCWWGG 4 cut(s) 186, 196, 764, 1018
Eco32I GATATC 1 cut(s) 1155
EcoRII CCWGG 5 cut(s) 351, 567, 601, 948, 1261
EcoRV GATATC 1 cut(s) 1155
EcoT14I CCWWGG 4 cut(s) 186, 196, 764, 1018
ErhI CCWWGG 4 cut(s) 186, 196, 764, 1018
FaeI CATG 4 cut(s) 377, 464, 915, 1097
FatI CATG 4 cut(s) 373, 460, 911, 1093
FauI CCCGC 1 cut(s) 665
FblI GTMKAC 3 cut(s) 51, 381, 1043
Fnu4HI GCNGC 2 cut(s) 94, 156
FokI GGATG 6 cut(s) 41, 77, 169, 850, 903, 986
Fsp4HI GCNGC 2 cut(s) 94, 156
FspBI CTAG 5 cut(s) 218, 239, 309, 551, 669
GluI GCNGC 2 cut(s) 94, 156
GsuI CTGGAG 3 cut(s) 302, 579, 624
HaeIII GGCC 3 cut(s) 601, 769, 1239
HapII CCGG 5 cut(s) 830, 964, 1039, 1175, 1246
Hin1II CATG 4 cut(s) 377, 464, 915, 1097
HinfI GANTC 8 cut(s) 101, 514, 564, 606, 711, 775, 790, 1184
HpaII CCGG 5 cut(s) 830, 964, 1039, 1175, 1246
HphI GGTGA 3 cut(s) 584, 880, 1160
Hpy166II GTNNAC 7 cut(s) 52, 382, 575, 682, 803, 847, 1044
Hpy188I TCNGA 7 cut(s) 100, 106, 439, 475, 716, 789, 1051
Hpy188III TCNNGA 9 cut(s) 281, 456, 486, 593, 794, 830, 920, 1175, 1229
Hpy8I GTNNAC 7 cut(s) 52, 382, 575, 682, 803, 847, 1044
HpyAV CCTTC 4 cut(s) 280, 827, 910, 1003
HpyCH4III ACNGT 3 cut(s) 707, 868, 1220
HpyCH4IV ACGT 1 cut(s) 726
HpyCH4V TGCA 4 cut(s) 429, 585, 635, 1093
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 748, 1079
HpyF3I CTNAG 5 cut(s) 97, 208, 438, 747, 788
HpySE526I ACGT 1 cut(s) 726
Hsp92II CATG 4 cut(s) 377, 464, 915, 1097
Kpn2I TCCGGA 2 cut(s) 829, 1174
Kzo9I GATC 4 cut(s) 289, 311, 595, 1207
LmnI GCTCC 1 cut(s) 1022
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 3 cut(s) 598, 914, 925
MabI ACCWGGT 1 cut(s) 351
MaeI CTAG 5 cut(s) 218, 239, 309, 551, 669
MaeII ACGT 1 cut(s) 726
MaeIII GTNAC 2 cut(s) 347, 390
MalI GATC 4 cut(s) 291, 313, 597, 1209
MboI GATC 4 cut(s) 289, 311, 595, 1207
MboII GAAGA 6 cut(s) 56, 190, 309, 323, 408, 1217
MhlI GDGCHC 1 cut(s) 888
MluCI AATT 4 cut(s) 71, 258, 276, 734
MlyI GAGTC 5 cut(s) 508, 615, 720, 799, 1193
MmeI TCCRAC 1 cut(s) 799
MnlI CCTC 6 cut(s) 19, 433, 493, 504, 554, 703
MroI TCCGGA 2 cut(s) 829, 1174
MroXI GAANNNNTTC 1 cut(s) 985
MseI TTAA 3 cut(s) 422, 878, 1269
MslI CAYNNNNRTG 3 cut(s) 910, 921, 1098
MspA1I CMGCKG 1 cut(s) 1082
MspI CCGG 5 cut(s) 830, 964, 1039, 1175, 1246
MspR9I CCNGG 5 cut(s) 353, 569, 603, 950, 1263
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 5 cut(s) 353, 569, 603, 950, 1263
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 161, 748, 1079
NdeII GATC 4 cut(s) 289, 311, 595, 1207
NlaIII CATG 4 cut(s) 377, 464, 915, 1097
NlaIV GGNNCC 3 cut(s) 468, 947, 1016
NmuCI GTSAC 1 cut(s) 390
NspI RCATGY 2 cut(s) 464, 1097
NspV TTCGAA 1 cut(s) 979
PciI ACATGT 1 cut(s) 460
PctI GAATGC 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 985
PfeI GAWTC 3 cut(s) 101, 564, 775
PkrI GCNGC 2 cut(s) 95, 157
PleI GAGTC 5 cut(s) 508, 614, 719, 798, 1192
PpsI GAGTC 5 cut(s) 508, 614, 719, 798, 1192
PscI ACATGT 1 cut(s) 460
Psp6I CCWGG 5 cut(s) 351, 567, 601, 948, 1261
PspGI CCWGG 5 cut(s) 351, 567, 601, 948, 1261
PspN4I GGNNCC 3 cut(s) 468, 947, 1016
PsrI GAACNNNNNNTAC 2 cut(s) 1241, 1273
PstNI CAGNNNCTG 2 cut(s) 104, 1001
RsaI GTAC 3 cut(s) 307, 526, 1213
RsaNI GTAC 3 cut(s) 306, 525, 1212
RseI CAYNNNNRTG 3 cut(s) 910, 921, 1098
SaqAI TTAA 3 cut(s) 422, 878, 1269
SatI GCNGC 2 cut(s) 94, 156
Sau3AI GATC 4 cut(s) 289, 311, 595, 1207
ScaI AGTACT 2 cut(s) 307, 526
SchI GAGTC 5 cut(s) 508, 615, 720, 799, 1193
ScrFI CCNGG 5 cut(s) 353, 569, 603, 950, 1263
SduI GDGCHC 1 cut(s) 888
SexAI ACCWGGT 1 cut(s) 351
SfaNI GCATC 3 cut(s) 598, 914, 925
SfuI TTCGAA 1 cut(s) 979
SmiMI CAYNNNNRTG 3 cut(s) 910, 921, 1098
SmlI CTYRAG 3 cut(s) 159, 516, 1229
SmoI CTYRAG 3 cut(s) 159, 516, 1229
Sse9I AATT 4 cut(s) 71, 258, 276, 734
SsiI CCGC 4 cut(s) 78, 93, 672, 1082
SspMI CTAG 5 cut(s) 218, 239, 309, 551, 669
StyD4I CCNGG 5 cut(s) 351, 567, 601, 948, 1261
StyI CCWWGG 4 cut(s) 186, 196, 764, 1018
TaaI ACNGT 3 cut(s) 707, 868, 1220
TaiI ACGT 1 cut(s) 729
TaqI TCGA 2 cut(s) 128, 979
TaqII GACCGA 1 cut(s) 1020
TasI AATT 4 cut(s) 71, 258, 276, 734
TatI WGTACW 2 cut(s) 305, 524
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 3 cut(s) 101, 564, 775
Tru1I TTAA 3 cut(s) 422, 878, 1269
Tru9I TTAA 3 cut(s) 422, 878, 1269
TscAI CASTG 1 cut(s) 321
TseFI GTSAC 1 cut(s) 390
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 390
TspDTI ATGAA 7 cut(s) 45, 84, 129, 323, 398, 408, 1134
TspGWI ACGGA 1 cut(s) 746
TspRI CASTG 1 cut(s) 321
XapI RAATTY 2 cut(s) 71, 734
XceI RCATGY 2 cut(s) 464, 1097
XmiI GTMKAC 3 cut(s) 51, 381, 1043
XmnI GAANNNNTTC 1 cut(s) 985
XspI CTAG 5 cut(s) 218, 239, 309, 551, 669
ZrmI AGTACT 2 cut(s) 307, 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.