Rh5BG512500

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001622
Physical Location & Seq
Reverse (-)
81349477 .. 81355631
6155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG512500.1

Sequence Viewer

Length: 945 bp
ATGCATTATAACAAAAGTTGTGGCATTGTTGATGAAATGGAAGGTGTTCATGATGATGTGGGTGGCGAACTTCGTGATAGAGAATTGGCTAGCTTGGAGTCTGATGCTCATGCAAGTAGAGGAGAAGACATCCAAGGAATTCCTTGGGATATACTTAGCATTACTAGAGAGAAATACCGGCAAACTAGGTTAGAACAATACAAGAATTATGAAAACATCCCTAATTCCGGAGAAGGGACAGGGAAGGACTGCAACATTACAGAAAAAGGATCTTCATATTATGAGTTTCAATGCAACACAAGATCAGTGAAACCAACTATCCTTCATTTTCAGTTGCGTAACTTGGTCTGGGCTACATCAAAGCATGATGTCTACCTTCTGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCGAAGCATCTGAGGTTCTAAATGTTTCAGGACATGTCACACCATCAGAGAAACATCCTGGAAGTCTATTGGAGGGACTTACACAGACTCAAGTTAGTAGTCTTGCAGTGAAAGATAAGTTGCTAGTTTCTGGAGGATTCCAAGGAGAACTTATTTGCAAGCATCTTGATCAACCTGGAGTTAGCTTTTGTTCGAGGACAACTGATGATGATAATGCCATTACAAATGCTATTGAGATTTATGACAATCCCAGTGGTGCAGCTCACTTTACAGCCTCAAGTAATGATTGTGGAGTCAGAGACTTTGACATGGAGACATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGTGAATCATACTTCTCTGAGTCCCGATGGTAAACTTCTTGTGATAGTTGGTGACAATCCAGATGGGATGTTGGTCGACTCTCAAACTGGAAAGGTGGTATTTAATTTACGCTTTTGTAGACTTACTTTAGAGCCAGGAATTGAAGGTTTACTGATATGGATGAGATTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

314

Amino Acids

35.07

Weight (kDa)

5.26

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 9
AccI GTMKAC 3 cut(s) 372, 846, 889
AccIII TCCGGA 1 cut(s) 227
AclWI GGATC 1 cut(s) 277
AcoI YGGCCR 1 cut(s) 767
AcsI RAATTY 1 cut(s) 138
AfiI CCNNNNNNNGG 2 cut(s) 227, 234
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 2 cut(s) 290, 914
AjnI CCWGG 3 cut(s) 475, 592, 904
AluBI AGCT 4 cut(s) 93, 603, 680, 742
AluI AGCT 4 cut(s) 93, 603, 680, 742
Alw26I GTCTC 2 cut(s) 711, 725
AlwI GGATC 1 cut(s) 277
Aor13HI TCCGGA 1 cut(s) 227
AoxI GGCC 1 cut(s) 767
ApeKI GCWGC 1 cut(s) 677
ApoI RAATTY 1 cut(s) 138
Asp700I GAANNNNTTC 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 833
AsuNHI GCTAGC 1 cut(s) 89
BalI TGGCCA 1 cut(s) 769
BbsI GAAGAC 2 cut(s) 132, 399
BbvI GCAGC 1 cut(s) 689
BccI CCATC 3 cut(s) 469, 791, 827
BciT130I CCWGG 3 cut(s) 477, 594, 906
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 2 cut(s) 711, 725
BfaI CTAG 4 cut(s) 90, 165, 186, 542
BisI GCNGC 1 cut(s) 678
BlsI GCNGC 1 cut(s) 679
Bme1390I CCNGG 3 cut(s) 477, 594, 906
BmrFI CCNGG 3 cut(s) 477, 594, 906
BmrI ACTGGG 1 cut(s) 663
BmsI GCATC 3 cut(s) 94, 434, 589
BmtI GCTAGC 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 663
BpiI GAAGAC 2 cut(s) 132, 399
BpmI CTGGAG 2 cut(s) 570, 615
BpuEI CTTGAG 2 cut(s) 492, 679
BsaJI CCNNGG 4 cut(s) 133, 143, 559, 764
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 234
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 3 cut(s) 669, 770, 862
BseAI TCCGGA 1 cut(s) 227
BseBI CCWGG 3 cut(s) 477, 594, 906
BseDI CCNNGG 4 cut(s) 133, 143, 559, 764
BseGI GGATG 5 cut(s) 129, 216, 472, 843, 936
BseLI CCNNNNNNNGG 2 cut(s) 227, 234
BseMII CTCAG 2 cut(s) 420, 779
BseNI ACTGG 3 cut(s) 669, 770, 862
BseRI GAGGAG 1 cut(s) 135
BseXI GCAGC 1 cut(s) 689
BsgI GTGCAG 1 cut(s) 696
BshFI GGCC 1 cut(s) 769
BsiSI CCGG 2 cut(s) 178, 228
BslFI GGGAC 3 cut(s) 250, 507, 777
BslI CCNNNNNNNGG 2 cut(s) 227, 234
BsmAI GTCTC 2 cut(s) 711, 725
BsmFI GGGAC 3 cut(s) 250, 507, 777
BsnI GGCC 1 cut(s) 769
Bsp13I TCCGGA 1 cut(s) 227
Bsp143I GATC 3 cut(s) 269, 302, 586
BspANI GGCC 1 cut(s) 769
BspCNI CTCAG 2 cut(s) 421, 780
BspEI TCCGGA 1 cut(s) 227
BspHI TCATGA 1 cut(s) 49
BspOI GCTAGC 1 cut(s) 93
BspPI GGATC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 177
BsrI ACTGG 3 cut(s) 669, 770, 862
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 4 cut(s) 133, 143, 559, 764
BssMI GATC 3 cut(s) 269, 302, 586
BssT1I CCWWGG 4 cut(s) 133, 143, 559, 764
Bst2UI CCWGG 3 cut(s) 477, 594, 906
BstC8I GCNNGC 2 cut(s) 91, 578
BstDEI CTNAG 4 cut(s) 155, 429, 747, 788
BstF5I GGATG 5 cut(s) 129, 216, 472, 843, 936
BstKTI GATC 3 cut(s) 272, 305, 589
BstMAI GTCTC 2 cut(s) 711, 725
BstMBI GATC 3 cut(s) 269, 302, 586
BstMWI GCNNNNNNNGC 1 cut(s) 748
BstNI CCWGG 3 cut(s) 477, 594, 906
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 3 cut(s) 475, 592, 904
BstV1I GCAGC 1 cut(s) 689
BstV2I GAAGAC 2 cut(s) 132, 399
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
BsuRI GGCC 1 cut(s) 769
BtsCI GGATG 5 cut(s) 129, 216, 472, 843, 936
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 4 cut(s) 312, 531, 676, 777
Cac8I GCNNGC 2 cut(s) 91, 578
CciI TCATGA 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 177
CspCI CAANNNNNGTGG 3 cut(s) 36, 652, 687
CviAII CATG 5 cut(s) 50, 110, 365, 452, 727
CviJI RGCY 9 cut(s) 89, 93, 353, 603, 680, 692, 742, 769, 904
CviKI_1 RGCY 9 cut(s) 89, 93, 353, 603, 680, 692, 742, 769, 904
DdeI CTNAG 4 cut(s) 155, 429, 747, 788
DpnI GATC 3 cut(s) 271, 304, 588
DpnII GATC 3 cut(s) 269, 302, 586
EaeI YGGCCR 1 cut(s) 767
Eco130I CCWWGG 4 cut(s) 133, 143, 559, 764
EcoRI GAATTC 1 cut(s) 138
EcoRII CCWGG 3 cut(s) 475, 592, 904
EcoT14I CCWWGG 4 cut(s) 133, 143, 559, 764
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 133, 143, 559, 764
FaeI CATG 5 cut(s) 53, 113, 368, 455, 730
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FaqI GGGAC 3 cut(s) 250, 507, 777
FatI CATG 5 cut(s) 49, 109, 364, 451, 726
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 3 cut(s) 372, 846, 889
Fnu4HI GCNGC 1 cut(s) 678
FokI GGATG 4 cut(s) 116, 203, 459, 850
Fsp4HI GCNGC 1 cut(s) 678
FspBI CTAG 4 cut(s) 90, 165, 186, 542
GluI GCNGC 1 cut(s) 678
GsuI CTGGAG 2 cut(s) 570, 615
HaeIII GGCC 1 cut(s) 769
HapII CCGG 2 cut(s) 178, 228
Hin1II CATG 5 cut(s) 53, 113, 368, 455, 730
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HinfI GANTC 8 cut(s) 98, 505, 555, 711, 775, 790, 848, 936
HpaII CCGG 2 cut(s) 178, 228
HphI GGTGA 1 cut(s) 833
Hpy166II GTNNAC 5 cut(s) 373, 803, 847, 890, 920
Hpy188I TCNGA 5 cut(s) 103, 430, 466, 716, 789
Hpy188III TCNNGA 8 cut(s) 50, 74, 228, 447, 549, 584, 794, 830
Hpy8I GTNNAC 5 cut(s) 373, 803, 847, 890, 920
HpyAV CCTTC 6 cut(s) 35, 227, 238, 332, 386, 908
HpyCH4V TGCA 7 cut(s) 4, 113, 252, 294, 524, 576, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 748
HpyF3I CTNAG 4 cut(s) 155, 429, 747, 788
Hsp92II CATG 5 cut(s) 53, 113, 368, 455, 730
Kpn2I TCCGGA 1 cut(s) 227
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 3 cut(s) 269, 302, 586
Lsp1109I GCAGC 1 cut(s) 689
LweI GCATC 3 cut(s) 94, 434, 589
MaeI CTAG 4 cut(s) 90, 165, 186, 542
MaeIII GTNAC 4 cut(s) 338, 381, 454, 821
MalI GATC 3 cut(s) 271, 304, 588
MboI GATC 3 cut(s) 269, 302, 586
MboII GAAGA 3 cut(s) 137, 264, 399
MflI RGATCY 1 cut(s) 269
MlsI TGGCCA 1 cut(s) 769
MluCI AATT 6 cut(s) 83, 138, 205, 223, 874, 909
MluNI TGGCCA 1 cut(s) 769
MlyI GAGTC 5 cut(s) 107, 499, 720, 799, 842
MnlI CCTC 6 cut(s) 113, 424, 484, 545, 606, 703
Mox20I TGGCCA 1 cut(s) 769
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 227
MroXI GAANNNNTTC 1 cut(s) 45
MscI TGGCCA 1 cut(s) 769
MseI TTAA 2 cut(s) 413, 873
MslI CAYNNNNRTG 1 cut(s) 54
Msp20I TGGCCA 1 cut(s) 769
MspI CCGG 2 cut(s) 178, 228
MspR9I CCNGG 3 cut(s) 477, 594, 906
MvaI CCWGG 3 cut(s) 477, 594, 906
MwoI GCNNNNNNNGC 1 cut(s) 748
NdeII GATC 3 cut(s) 269, 302, 586
NheI GCTAGC 1 cut(s) 89
NlaIII CATG 5 cut(s) 53, 113, 368, 455, 730
NmuCI GTSAC 3 cut(s) 381, 454, 821
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 455
PagI TCATGA 1 cut(s) 49
PciI ACATGT 1 cut(s) 451
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 3 cut(s) 555, 775, 936
PfoI TCCNGGA 1 cut(s) 475
PkrI GCNGC 1 cut(s) 679
PleI GAGTC 5 cut(s) 106, 499, 719, 798, 842
PpsI GAGTC 5 cut(s) 106, 499, 719, 798, 842
PscI ACATGT 1 cut(s) 451
PsiI TTATAA 1 cut(s) 9
Psp6I CCWGG 3 cut(s) 475, 592, 904
PspGI CCWGG 3 cut(s) 475, 592, 904
PsuI RGATCY 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 54
SalI GTCGAC 1 cut(s) 845
SaqAI TTAA 2 cut(s) 413, 873
SatI GCNGC 1 cut(s) 678
Sau3AI GATC 3 cut(s) 269, 302, 586
SchI GAGTC 5 cut(s) 107, 499, 720, 799, 842
ScrFI CCNGG 3 cut(s) 477, 594, 906
SfaNI GCATC 3 cut(s) 94, 434, 589
SmiMI CAYNNNNRTG 1 cut(s) 54
SmlI CTYRAG 2 cut(s) 507, 694
SmoI CTYRAG 2 cut(s) 507, 694
Sse9I AATT 6 cut(s) 83, 138, 205, 223, 874, 909
SspMI CTAG 4 cut(s) 90, 165, 186, 542
StyD4I CCNGG 3 cut(s) 475, 592, 904
StyI CCWWGG 4 cut(s) 133, 143, 559, 764
TaqI TCGA 2 cut(s) 611, 846
TasI AATT 6 cut(s) 83, 138, 205, 223, 874, 909
TfiI GAWTC 3 cut(s) 555, 775, 936
Tru1I TTAA 2 cut(s) 413, 873
Tru9I TTAA 2 cut(s) 413, 873
TscAI CASTG 4 cut(s) 312, 531, 676, 777
TseFI GTSAC 3 cut(s) 381, 454, 821
TseI GCWGC 1 cut(s) 677
Tsp45I GTSAC 3 cut(s) 381, 454, 821
TspDTI ATGAA 7 cut(s) 38, 48, 225, 264, 314, 389, 399
TspGWI ACGGA 1 cut(s) 746
TspRI CASTG 4 cut(s) 312, 531, 676, 777
XapI RAATTY 1 cut(s) 138
XceI RCATGY 1 cut(s) 455
XmiI GTMKAC 3 cut(s) 372, 846, 889
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 4 cut(s) 90, 165, 186, 542
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.