Rw5G045450

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001622
Physical Location & Seq
Reverse (-)
79473604 .. 79478060
4457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045450.1

Sequence Viewer

Length: 1329 bp
ATGTCCCACTACGCTGGTTATGTGGAAGATGAATACGAAATGGAAGATGTAGATGATGATATGGATGACGAATTTCGTGGAAGAGACCTGGCTGGCTCCGAGTCTGATGTTGATGAATTTGAATATGCGAATAGTAAAGTAGCTGATACAACTGCTGCTCAAGCAAGAAGAGGAAAAGACATCCAAGGTATTCCTTGGGATAGACTTAGCATTACTAGAGAAAAATACCGGCAAACTAGGTTAGAACAGTACAAGAATTATGAAAACATCCCTAATTCCGGAGAAGGGTCAGGGAAGGATTGCAACATTACAAAGAAGGGATCTGTATATTATGAGTTTAGACGTAATGCAAGATCAGTGAAATCAACTATTCTTCATTTTCAGTTGCGGAACTTGGTCTGGGCTACATCAAAGCATGATGTCTACCTTATGTCACATTTTTCTGTCATTCATTGGTCTTCATTGAGTTGCAACAAGTCTGAGGTTCTAAATGTTTCAGGACATGTGGCACCATCAGAGAAACATCCCGGGAGTCTCTTGGAGGGATTTACGCAGACTCAAGTTAGTACTCTTGCAGTAAAAGATAAGTTGCTGGTTGCTGGAGGATTCCAGGGAGAACTTATTTGTAAGCATCTTGATCGGCCTGGAGTTAGCTTTTGTTCAAGGACAACTTACGATGATAATGCCATTACAAATGCTGTTGAGATTTATGATGGTCCCAGTGGTGCAGTTCACTTTACAGCATCAAATAATGATTGTGGAGTCAGAGACTTTGACATGGAGAAATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGTGAATCATACTTCTTTGAGTCCCAGTGGCAAACTTCTTGTGATTGTTGGTGACAATCCAGATGGGATGTTGGTGGACTCTCAGACTGGAAAGACAGTGGCGGCTTTATCTGGGCACTTGGATTTCTCATTTGCATCAGCGTGGCATCCTGATGGTATCACCTTTGCTACTGGGAACCAGGACAAAACTTGTCGGGTTTGGGATGTTCGAAACCTATCCAAGTCGGTTGCTGTTTTAAAGGGAAACCTTGGAGCTATTCGGTCAATCCGTTATACTTCTGATGGTCGGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTATGAGAAGGAGCAGGAAATCGACTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCAGACACAGAGTCACTTTTCATTGGAGTGTGGGATCGTACGTATGGTAGTCTTCTCGAGTATGGACGACACCGGGATTACTCTTACCTAGATTCTCTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

442

Amino Acids

49.66

Weight (kDa)

5.34

Isoelectric Point (pI)

28.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 194 - 389 9.1e-16 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 244 - 391 1.4e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 246 - 347 7.4e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 248 - 414 2.1e-18 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 248 - 422 2.4e-14 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 248 - 344 8.2e-06 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 277 - 389 7.9e-08 WDR55
WD40_CDC20-Fz PF24807 278 - 414 5.5e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 279 - 421 7.8e-10 WDR3 first beta-propeller domain
WD40 PF00400 304 - 342 6.7e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 423
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 2 cut(s) 388, 923
AclWI GGATC 2 cut(s) 328, 1266
AcoI YGGCCR 1 cut(s) 818
AcsI RAATTY 3 cut(s) 71, 116, 785
AfaI GTAC 3 cut(s) 251, 568, 1264
AfiI CCNNNNNNNGG 2 cut(s) 278, 285
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 2 cut(s) 122, 663
AhdI GACNNNNNGTC 1 cut(s) 1234
AjnI CCWGG 4 cut(s) 87, 609, 643, 999
AluBI AGCT 4 cut(s) 143, 654, 793, 1076
AluI AGCT 4 cut(s) 143, 654, 793, 1076
Alw26I GTCTC 3 cut(s) 78, 539, 762
AlwI GGATC 2 cut(s) 328, 1266
Ama87I CYCGRG 2 cut(s) 527, 1280
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 2 cut(s) 641, 818
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 3 cut(s) 71, 116, 785
AspS9I GGNCC 1 cut(s) 716
AsuC2I CCSGG 3 cut(s) 528, 529, 1298
AsuHPI GGTGA 3 cut(s) 884, 973, 1211
AsuII TTCGAA 1 cut(s) 1030
AvaI CYCGRG 2 cut(s) 527, 1280
AvaII GGWCC 1 cut(s) 716
BaeGI GKGCMC 1 cut(s) 939
BalI TGGCCA 1 cut(s) 820
BanI GGYRCC 1 cut(s) 508
BbsI GAAGAC 2 cut(s) 450, 1268
BbvI GCAGC 1 cut(s) 142
BccI CCATC 6 cut(s) 520, 707, 878, 968, 1097, 1114
BcgI CGANNNNNNTGC 4 cut(s) 620, 654, 665, 699
BciT130I CCWGG 4 cut(s) 89, 611, 645, 1001
BcnI CCSGG 3 cut(s) 528, 529, 1298
BcoDI GTCTC 3 cut(s) 78, 539, 762
BfaI CTAG 3 cut(s) 216, 237, 1313
BisI GCNGC 2 cut(s) 156, 924
BlsI GCNGC 2 cut(s) 157, 925
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 7 cut(s) 89, 528, 529, 611, 645, 1001, 1298
Bme18I GGWCC 1 cut(s) 716
BmeRI GACNNNNNGTC 1 cut(s) 1234
BmeT110I CYCGRG 2 cut(s) 527, 1280
BmgT120I GGNCC 1 cut(s) 716
BmiI GGNNCC 4 cut(s) 97, 510, 718, 998
BmrFI CCNGG 7 cut(s) 89, 528, 529, 611, 645, 1001, 1298
BmrI ACTGGG 3 cut(s) 714, 840, 1002
BmsI GCATC 4 cut(s) 640, 752, 965, 976
BmuI ACTGGG 3 cut(s) 714, 840, 1002
BpiI GAAGAC 2 cut(s) 450, 1268
BpmI CTGGAG 2 cut(s) 621, 666
Bpu14I TTCGAA 1 cut(s) 1030
BpuEI CTTGAG 2 cut(s) 144, 543
BpuMI CCSGG 3 cut(s) 528, 529, 1298
BsaAI YACGTR 1 cut(s) 1266
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 6 cut(s) 184, 194, 527, 610, 815, 1069
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 285
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 5 cut(s) 720, 821, 846, 913, 997
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 4 cut(s) 89, 611, 645, 1001
BseDI CCNNGG 6 cut(s) 184, 194, 527, 610, 815, 1069
BseGI GGATG 7 cut(s) 70, 180, 267, 523, 894, 967, 1030
BseLI CCNNNNNNNGG 2 cut(s) 278, 285
BseMII CTCAG 2 cut(s) 471, 917
BseNI ACTGG 5 cut(s) 720, 821, 846, 913, 997
BseSI GKGCMC 1 cut(s) 939
BseXI GCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 2 cut(s) 643, 820
BshNI GGYRCC 1 cut(s) 508
BsiHKCI CYCGRG 2 cut(s) 527, 1280
BsiSI CCGG 4 cut(s) 229, 279, 528, 1297
BsiWI CGTACG 1 cut(s) 1262
BslFI GGGAC 2 cut(s) 702, 828
BslI CCNNNNNNNGG 2 cut(s) 278, 285
BsmAI GTCTC 3 cut(s) 78, 539, 762
BsmFI GGGAC 2 cut(s) 702, 828
BsnI GGCC 2 cut(s) 643, 820
Bso31I GGTCTC 1 cut(s) 78
BsoBI CYCGRG 2 cut(s) 527, 1280
Bsp119I TTCGAA 1 cut(s) 1030
Bsp1286I GDGCHC 1 cut(s) 939
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 4 cut(s) 320, 353, 637, 1258
BspACI CCGC 2 cut(s) 388, 923
BspANI GGCC 2 cut(s) 643, 820
BspCNI CTCAG 2 cut(s) 472, 916
BspEI TCCGGA 1 cut(s) 278
BspLI GGNNCC 4 cut(s) 97, 510, 718, 998
BspPI GGATC 2 cut(s) 328, 1266
BspT104I TTCGAA 1 cut(s) 1030
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 5 cut(s) 720, 821, 846, 913, 997
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 6 cut(s) 184, 194, 527, 610, 815, 1069
BssMI GATC 4 cut(s) 320, 353, 637, 1258
BssT1I CCWWGG 4 cut(s) 184, 194, 815, 1069
Bst2UI CCWGG 4 cut(s) 89, 611, 645, 1001
Bst4CI ACNGT 2 cut(s) 249, 919
Bst6I CTCTTC 2 cut(s) 76, 163
BstBAI YACGTR 1 cut(s) 1266
BstBI TTCGAA 1 cut(s) 1030
BstC8I GCNNGC 2 cut(s) 94, 1131
BstDEI CTNAG 4 cut(s) 206, 480, 798, 903
BstF5I GGATG 7 cut(s) 70, 180, 267, 523, 894, 967, 1030
BstKTI GATC 4 cut(s) 323, 356, 640, 1261
BstMAI GTCTC 3 cut(s) 78, 539, 762
BstMBI GATC 4 cut(s) 320, 353, 637, 1258
BstMWI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
BstNI CCWGG 4 cut(s) 89, 611, 645, 1001
BstNSI RCATGY 2 cut(s) 506, 1148
BstSCI CCNGG 7 cut(s) 87, 526, 527, 609, 643, 999, 1296
BstSLI GKGCMC 1 cut(s) 939
BstSNI TACGTA 1 cut(s) 1266
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 2 cut(s) 450, 1268
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 14
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 2 cut(s) 643, 820
BtsCI GGATG 7 cut(s) 70, 180, 267, 523, 894, 967, 1030
BtsIMutI CAGTG 5 cut(s) 363, 727, 828, 853, 924
Cac8I GCNNGC 2 cut(s) 94, 1131
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 716
Cfr9I CCCGGG 1 cut(s) 527
Csp6I GTAC 3 cut(s) 250, 567, 1263
CviAII CATG 4 cut(s) 416, 503, 778, 1145
CviQI GTAC 3 cut(s) 250, 567, 1263
DdeI CTNAG 4 cut(s) 206, 480, 798, 903
DpnI GATC 4 cut(s) 322, 355, 639, 1260
DpnII GATC 4 cut(s) 320, 353, 637, 1258
DraI TTTAAA 1 cut(s) 1059
DriI GACNNNNNGTC 1 cut(s) 1234
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 2 cut(s) 76, 163
Eam1105I GACNNNNNGTC 1 cut(s) 1234
EarI CTCTTC 2 cut(s) 76, 163
Eco105I TACGTA 1 cut(s) 1266
Eco130I CCWWGG 4 cut(s) 184, 194, 815, 1069
Eco31I GGTCTC 1 cut(s) 78
Eco32I GATATC 1 cut(s) 1206
Eco47I GGWCC 1 cut(s) 716
Eco88I CYCGRG 2 cut(s) 527, 1280
EcoRII CCWGG 4 cut(s) 87, 609, 643, 999
EcoRV GATATC 1 cut(s) 1206
EcoT14I CCWWGG 4 cut(s) 184, 194, 815, 1069
ErhI CCWWGG 4 cut(s) 184, 194, 815, 1069
FaeI CATG 4 cut(s) 419, 506, 781, 1148
FalI AAGNNNNNCTT 2 cut(s) 655, 687
FaqI GGGAC 2 cut(s) 702, 828
FatI CATG 4 cut(s) 415, 502, 777, 1144
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 156, 924
FokI GGATG 7 cut(s) 77, 167, 254, 510, 901, 954, 1037
Fsp4HI GCNGC 2 cut(s) 156, 924
FspBI CTAG 3 cut(s) 216, 237, 1313
GluI GCNGC 2 cut(s) 156, 924
GsuI CTGGAG 2 cut(s) 621, 666
HaeIII GGCC 2 cut(s) 643, 820
HapII CCGG 4 cut(s) 229, 279, 528, 1297
Hin1II CATG 4 cut(s) 419, 506, 781, 1148
HpaII CCGG 4 cut(s) 229, 279, 528, 1297
HphI GGTGA 3 cut(s) 884, 973, 1211
Hpy166II GTNNAC 3 cut(s) 424, 733, 898
Hpy188I TCNGA 7 cut(s) 100, 106, 481, 517, 767, 906, 1102
Hpy188III TCNNGA 7 cut(s) 279, 498, 635, 881, 971, 1226, 1280
Hpy8I GTNNAC 3 cut(s) 424, 733, 898
HpyAV CCTTC 4 cut(s) 278, 289, 310, 1168
HpyCH4III ACNGT 2 cut(s) 249, 919
HpyCH4IV ACGT 2 cut(s) 343, 1265
HpyCH4V TGCA 7 cut(s) 303, 350, 471, 575, 728, 956, 1144
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
HpyF3I CTNAG 4 cut(s) 206, 480, 798, 903
HpySE526I ACGT 2 cut(s) 343, 1265
Hsp92II CATG 4 cut(s) 419, 506, 781, 1148
Kpn2I TCCGGA 1 cut(s) 278
Kzo9I GATC 4 cut(s) 320, 353, 637, 1258
LmnI GCTCC 3 cut(s) 101, 1073, 1177
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 4 cut(s) 640, 752, 965, 976
MaeI CTAG 3 cut(s) 216, 237, 1313
MaeII ACGT 2 cut(s) 343, 1265
MaeIII GTNAC 3 cut(s) 432, 872, 1236
MalI GATC 4 cut(s) 322, 355, 639, 1260
MboI GATC 4 cut(s) 320, 353, 637, 1258
MboII GAAGA 7 cut(s) 38, 56, 93, 180, 365, 450, 1268
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 1 cut(s) 939
MlsI TGGCCA 1 cut(s) 820
MluCI AATT 5 cut(s) 71, 116, 256, 274, 785
MluNI TGGCCA 1 cut(s) 820
MlyI GAGTC 7 cut(s) 110, 541, 550, 771, 850, 893, 1244
MnlI CCTC 4 cut(s) 164, 475, 535, 596
Mox20I TGGCCA 1 cut(s) 820
MroI TCCGGA 1 cut(s) 278
MscI TGGCCA 1 cut(s) 820
MseI TTAA 2 cut(s) 1058, 1327
MslI CAYNNNNRTG 4 cut(s) 961, 972, 1149, 1250
Msp20I TGGCCA 1 cut(s) 820
MspI CCGG 4 cut(s) 229, 279, 528, 1297
MspR9I CCNGG 7 cut(s) 89, 528, 529, 611, 645, 1001, 1298
MvaI CCWGG 4 cut(s) 89, 611, 645, 1001
MwoI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
NciI CCSGG 3 cut(s) 528, 529, 1298
NdeII GATC 4 cut(s) 320, 353, 637, 1258
NlaIII CATG 4 cut(s) 419, 506, 781, 1148
NlaIV GGNNCC 4 cut(s) 97, 510, 718, 998
NmuCI GTSAC 3 cut(s) 432, 872, 1236
NspI RCATGY 2 cut(s) 506, 1148
NspV TTCGAA 1 cut(s) 1030
PaeR7I CTCGAG 1 cut(s) 1280
PciI ACATGT 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 1087
PfeI GAWTC 3 cut(s) 606, 826, 1316
Pfl23II CGTACG 1 cut(s) 1262
PkrI GCNGC 2 cut(s) 157, 925
PleI GAGTC 7 cut(s) 109, 540, 550, 770, 849, 893, 1243
PpsI GAGTC 7 cut(s) 109, 540, 550, 770, 849, 893, 1243
Ppu21I YACGTR 1 cut(s) 1266
PscI ACATGT 1 cut(s) 502
Psp6I CCWGG 4 cut(s) 87, 609, 643, 999
PspGI CCWGG 4 cut(s) 87, 609, 643, 999
PspLI CGTACG 1 cut(s) 1262
PspN4I GGNNCC 4 cut(s) 97, 510, 718, 998
PspPI GGNCC 1 cut(s) 716
PsuI RGATCY 1 cut(s) 320
RsaI GTAC 3 cut(s) 251, 568, 1264
RsaNI GTAC 3 cut(s) 250, 567, 1263
RseI CAYNNNNRTG 4 cut(s) 961, 972, 1149, 1250
SaqAI TTAA 2 cut(s) 1058, 1327
SatI GCNGC 2 cut(s) 156, 924
Sau3AI GATC 4 cut(s) 320, 353, 637, 1258
Sau96I GGNCC 1 cut(s) 716
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 7 cut(s) 110, 541, 550, 771, 850, 893, 1244
ScrFI CCNGG 7 cut(s) 89, 528, 529, 611, 645, 1001, 1298
SduI GDGCHC 1 cut(s) 939
SfaNI GCATC 4 cut(s) 640, 752, 965, 976
Sfr274I CTCGAG 1 cut(s) 1280
SfuI TTCGAA 1 cut(s) 1030
SinI GGWCC 1 cut(s) 716
SlaI CTCGAG 1 cut(s) 1280
SmaI CCCGGG 1 cut(s) 529
SmiMI CAYNNNNRTG 4 cut(s) 961, 972, 1149, 1250
SmlI CTYRAG 3 cut(s) 159, 558, 1280
SmoI CTYRAG 3 cut(s) 159, 558, 1280
SnaBI TACGTA 1 cut(s) 1266
Sse9I AATT 5 cut(s) 71, 116, 256, 274, 785
SsiI CCGC 2 cut(s) 388, 923
SspMI CTAG 3 cut(s) 216, 237, 1313
StyD4I CCNGG 7 cut(s) 87, 526, 527, 609, 643, 999, 1296
StyI CCWWGG 4 cut(s) 184, 194, 815, 1069
TaaI ACNGT 2 cut(s) 249, 919
TaiI ACGT 2 cut(s) 346, 1268
TaqI TCGA 3 cut(s) 1030, 1188, 1281
TaqII GACCGA 1 cut(s) 1071
TasI AATT 5 cut(s) 71, 116, 256, 274, 785
TatI WGTACW 2 cut(s) 249, 566
TauI GCSGC 1 cut(s) 926
TfiI GAWTC 3 cut(s) 606, 826, 1316
Tru1I TTAA 2 cut(s) 1058, 1327
Tru9I TTAA 2 cut(s) 1058, 1327
TscAI CASTG 5 cut(s) 363, 727, 828, 853, 924
TseFI GTSAC 3 cut(s) 432, 872, 1236
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 3 cut(s) 432, 872, 1236
TspDTI ATGAA 7 cut(s) 45, 129, 276, 365, 440, 450, 1234
TspGWI ACGGA 2 cut(s) 797, 1079
TspMI CCCGGG 1 cut(s) 527
TspRI CASTG 5 cut(s) 363, 727, 828, 853, 924
VpaK11BI GGWCC 1 cut(s) 716
XapI RAATTY 3 cut(s) 71, 116, 785
XceI RCATGY 2 cut(s) 506, 1148
XhoI CTCGAG 1 cut(s) 1280
XmaI CCCGGG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 3 cut(s) 216, 237, 1313
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.