RchiOBHm_Chr5g0074741

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001622
Physical Location & Seq
Reverse (-)
80704558 .. 80707348
2791 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34952

Sequence Viewer

Length: 1161 bp
ATGATTACTGATATTTGTGGCATTGTTGATGAAATGGAAGGTGTTCATGATGATATGGGTGGCGAACTTTGTGATAGAGAATTGGCTAGCTTGGAGTCTGATGCTCATGCAAGTAGAGGAGAAGACATCCAAGGTATTCCTTGGGATAGACTTAGCATTACTAGAGAGAAATACCGGCAAACTAGGTTAGAACAATACAAGAATTATGAAAACATCCCTAATTCCGGAGAAGGGACAAGGAAGTTGCGTAACTTGGTCTGGGCTACATCAAAGTATGATGTCTACCTTCTGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCGAAGCATCTGAGGTTCTAAATGTTTCAGGACATGTCACACCATCGGAGAAACATCCTGGAAGTCTCTTGGAGGGACTTACACAGACTGAAGTTAGTAGTCTTGCAGTGAAAGATAAGTTGCTAGTTTCTGGAGGATTCCAAGGAGAACTTATTTGCAAGCATCTTGATCAACCTGGAGTTAGCTTTTGTTCGAGGACAACTGATGATGATAGTGCCATTACAAATGCTATTGAGATTTATGACAATCCCAGTGGTGCAGCTCACTTTACAGCCTCAACTAATGATTGTGGAGTCAGAGACTTTGACATGGAGACATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGTGAATGTTCCCGATGGTAAACTTCTTGTGACAGTTGGTGACAATCCAGATGGGATGTTGGTCGACTCTCAAACTGGAAAGGTGACAGTGATGTCTTTATGTGGACACTTGGATTTCTCGTTCGCATTAGCGTGGCATCCTGATGGTGTTACCTTTGCTACTGGGAACCAGGATAAGACCTGTTGGGTTTGCGATGTTCGAAACCTATCCAAGTCAGTTGCTGTTTTAAAGGGAAACCTTGGAGCTATTCAGTCAATTCGTTATACTTCTGATGGTCAGTATATGATGGCGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTACGAGAAGGAGCAGGAAATCGATTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCAGACACAGAGTCGCTTTTCATTGGAGTATGGGATCGTACATATGGTAGTCTTCTCGAGTATGGAAGACAAAAGTGTGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

386

Amino Acids

42.69

Weight (kDa)

4.75

Isoelectric Point (pI)

23.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 231 - 371 2.8e-13 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 231 - 385 1.6e-10 WDR5 beta-propeller domain
WD40_Prp19 PF24814 231 - 339 4.9e-10 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 231 - 347 1e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 232 - 364 1.1e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 249 - 371 1.4e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 772
AccI GTMKAC 2 cut(s) 282, 744
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 1 cut(s) 971
AclWI GGATC 1 cut(s) 1116
AcoI YGGCCR 1 cut(s) 677
AcuI CTGAAG 1 cut(s) 438
AfaI GTAC 2 cut(s) 1019, 1114
AfiI CCNNNNNNNGG 2 cut(s) 224, 231
AflIII ACRYGT 1 cut(s) 361
AhdI GACNNNNNGTC 1 cut(s) 1084
AjnI CCWGG 3 cut(s) 385, 502, 849
AloI GAACNNNNNNTCC 2 cut(s) 785, 817
AluBI AGCT 5 cut(s) 90, 513, 590, 652, 926
AluI AGCT 5 cut(s) 90, 513, 590, 652, 926
Alw26I GTCTC 3 cut(s) 398, 621, 635
AlwI GGATC 1 cut(s) 1116
AlwNI CAGNNNCTG 1 cut(s) 902
Ama87I CYCGRG 1 cut(s) 1130
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 1 cut(s) 677
ApeKI GCWGC 1 cut(s) 587
Asp700I GAANNNNTTC 1 cut(s) 42
AsuHPI GGTGA 3 cut(s) 731, 775, 1061
AsuII TTCGAA 1 cut(s) 880
AsuNHI GCTAGC 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 1130
BalI TGGCCA 1 cut(s) 679
BbsI GAAGAC 4 cut(s) 129, 309, 1118, 1147
BbvI GCAGC 1 cut(s) 599
BccI CCATC 6 cut(s) 379, 689, 725, 818, 947, 961
BciT130I CCWGG 3 cut(s) 387, 504, 851
BclI TGATCA 1 cut(s) 496
BcoDI GTCTC 3 cut(s) 398, 621, 635
BfaI CTAG 4 cut(s) 87, 162, 183, 452
BisI GCNGC 2 cut(s) 588, 972
BlsI GCNGC 2 cut(s) 589, 973
Bme1390I CCNGG 3 cut(s) 387, 504, 851
BmeRI GACNNNNNGTC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 1130
BmiI GGNNCC 1 cut(s) 848
BmrFI CCNGG 3 cut(s) 387, 504, 851
BmrI ACTGGG 2 cut(s) 573, 852
BmsI GCATC 4 cut(s) 91, 344, 499, 826
BmtI GCTAGC 1 cut(s) 90
BmuI ACTGGG 2 cut(s) 573, 852
BpiI GAAGAC 4 cut(s) 129, 309, 1118, 1147
BpmI CTGGAG 2 cut(s) 480, 525
Bpu14I TTCGAA 1 cut(s) 880
Bsa29I ATCGAT 1 cut(s) 1038
BsaJI CCNNGG 5 cut(s) 130, 140, 469, 674, 919
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 231
Bse118I RCCGGY 1 cut(s) 174
Bse1I ACTGG 4 cut(s) 579, 680, 760, 847
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 3 cut(s) 387, 504, 851
BseCI ATCGAT 1 cut(s) 1038
BseDI CCNNGG 5 cut(s) 130, 140, 469, 674, 919
BseGI GGATG 5 cut(s) 126, 213, 382, 741, 817
BseLI CCNNNNNNNGG 2 cut(s) 224, 231
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 4 cut(s) 579, 680, 760, 847
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 599
BsgI GTGCAG 1 cut(s) 606
BshFI GGCC 1 cut(s) 679
BshVI ATCGAT 1 cut(s) 1038
BsiHKCI CYCGRG 1 cut(s) 1130
BsiSI CCGG 2 cut(s) 175, 225
BslFI GGGAC 2 cut(s) 247, 417
BslI CCNNNNNNNGG 2 cut(s) 224, 231
BsmAI GTCTC 3 cut(s) 398, 621, 635
BsmFI GGGAC 2 cut(s) 247, 417
BsnI GGCC 1 cut(s) 679
BsoBI CYCGRG 1 cut(s) 1130
Bsp119I TTCGAA 1 cut(s) 880
Bsp13I TCCGGA 1 cut(s) 224
Bsp143I GATC 2 cut(s) 496, 1108
BspACI CCGC 1 cut(s) 971
BspANI GGCC 1 cut(s) 679
BspCNI CTCAG 1 cut(s) 331
BspDI ATCGAT 1 cut(s) 1038
BspEI TCCGGA 1 cut(s) 224
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 1 cut(s) 848
BspOI GCTAGC 1 cut(s) 90
BspPI GGATC 1 cut(s) 1116
BspT104I TTCGAA 1 cut(s) 880
BsrFI RCCGGY 1 cut(s) 174
BsrI ACTGG 4 cut(s) 579, 680, 760, 847
BssAI RCCGGY 1 cut(s) 174
BssECI CCNNGG 5 cut(s) 130, 140, 469, 674, 919
BssMI GATC 2 cut(s) 496, 1108
BssT1I CCWWGG 5 cut(s) 130, 140, 469, 674, 919
Bst2UI CCWGG 3 cut(s) 387, 504, 851
Bst4CI ACNGT 2 cut(s) 715, 769
BstBI TTCGAA 1 cut(s) 880
BstC8I GCNNGC 3 cut(s) 88, 488, 981
BstDEI CTNAG 3 cut(s) 152, 339, 657
BstF5I GGATG 5 cut(s) 126, 213, 382, 741, 817
BstKTI GATC 2 cut(s) 499, 1111
BstMAI GTCTC 3 cut(s) 398, 621, 635
BstMBI GATC 2 cut(s) 496, 1108
BstMWI GCNNNNNNNGC 2 cut(s) 658, 980
BstNI CCWGG 3 cut(s) 387, 504, 851
BstNSI RCATGY 2 cut(s) 365, 998
BstSCI CCNGG 3 cut(s) 385, 502, 849
BstV1I GCAGC 1 cut(s) 599
BstV2I GAAGAC 4 cut(s) 129, 309, 1118, 1147
Bsu15I ATCGAT 1 cut(s) 1038
BsuRI GGCC 1 cut(s) 679
BsuTUI ATCGAT 1 cut(s) 1038
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 5 cut(s) 126, 213, 382, 741, 817
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 4 cut(s) 441, 586, 687, 774
Cac8I GCNNGC 3 cut(s) 88, 488, 981
CaiI CAGNNNCTG 1 cut(s) 902
CciI TCATGA 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 1038
Csp6I GTAC 2 cut(s) 1018, 1113
CspCI CAANNNNNGTGG 2 cut(s) 562, 597
CviAII CATG 5 cut(s) 47, 107, 362, 637, 995
CviQI GTAC 2 cut(s) 1018, 1113
DdeI CTNAG 3 cut(s) 152, 339, 657
DpnI GATC 2 cut(s) 498, 1110
DpnII GATC 2 cut(s) 496, 1108
DraI TTTAAA 1 cut(s) 909
DrdI GACNNNNNNGTC 1 cut(s) 772
DriI GACNNNNNGTC 1 cut(s) 1084
DseDI GACNNNNNNGTC 1 cut(s) 772
EaeI YGGCCR 1 cut(s) 677
Eam1105I GACNNNNNGTC 1 cut(s) 1084
Eco130I CCWWGG 5 cut(s) 130, 140, 469, 674, 919
Eco32I GATATC 1 cut(s) 1056
Eco57I CTGAAG 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 1130
EcoRII CCWGG 3 cut(s) 385, 502, 849
EcoRV GATATC 1 cut(s) 1056
EcoT14I CCWWGG 5 cut(s) 130, 140, 469, 674, 919
ErhI CCWWGG 5 cut(s) 130, 140, 469, 674, 919
FaeI CATG 5 cut(s) 50, 110, 365, 640, 998
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 2 cut(s) 247, 417
FatI CATG 5 cut(s) 46, 106, 361, 636, 994
FauNDI CATATG 1 cut(s) 1117
FbaI TGATCA 1 cut(s) 496
FblI GTMKAC 2 cut(s) 282, 744
Fnu4HI GCNGC 2 cut(s) 588, 972
FokI GGATG 5 cut(s) 113, 200, 369, 748, 804
Fsp4HI GCNGC 2 cut(s) 588, 972
FspBI CTAG 4 cut(s) 87, 162, 183, 452
GluI GCNGC 2 cut(s) 588, 972
GsuI CTGGAG 2 cut(s) 480, 525
HaeIII GGCC 1 cut(s) 679
HapII CCGG 2 cut(s) 175, 225
Hin1II CATG 5 cut(s) 50, 110, 365, 640, 998
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HinfI GANTC 5 cut(s) 95, 465, 621, 746, 1085
HpaII CCGG 2 cut(s) 175, 225
HphI GGTGA 3 cut(s) 731, 775, 1061
Hpy166II GTNNAC 4 cut(s) 283, 701, 745, 785
Hpy188I TCNGA 5 cut(s) 100, 340, 376, 626, 952
Hpy8I GTNNAC 4 cut(s) 283, 701, 745, 785
HpyAV CCTTC 4 cut(s) 32, 224, 296, 1018
HpyCH4III ACNGT 2 cut(s) 715, 769
HpyCH4V TGCA 5 cut(s) 110, 434, 486, 587, 994
HpyF10VI GCNNNNNNNGC 2 cut(s) 658, 980
HpyF3I CTNAG 3 cut(s) 152, 339, 657
Hsp92II CATG 5 cut(s) 50, 110, 365, 640, 998
Kpn2I TCCGGA 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 496
Kzo9I GATC 2 cut(s) 496, 1108
LmnI GCTCC 2 cut(s) 923, 1027
Lsp1109I GCAGC 1 cut(s) 599
LweI GCATC 4 cut(s) 91, 344, 499, 826
MaeI CTAG 4 cut(s) 87, 162, 183, 452
MaeIII GTNAC 7 cut(s) 248, 291, 364, 709, 719, 763, 829
MalI GATC 2 cut(s) 498, 1110
MboI GATC 2 cut(s) 496, 1108
MboII GAAGA 4 cut(s) 134, 309, 1118, 1152
MlsI TGGCCA 1 cut(s) 679
MluCI AATT 4 cut(s) 80, 202, 220, 936
MluNI TGGCCA 1 cut(s) 679
MlyI GAGTC 4 cut(s) 104, 630, 740, 1094
MnlI CCTC 6 cut(s) 110, 334, 394, 455, 516, 613
Mox20I TGGCCA 1 cut(s) 679
MroI TCCGGA 1 cut(s) 224
MroXI GAANNNNTTC 1 cut(s) 42
MscI TGGCCA 1 cut(s) 679
MseI TTAA 3 cut(s) 323, 908, 1159
MslI CAYNNNNRTG 3 cut(s) 811, 822, 999
Msp20I TGGCCA 1 cut(s) 679
MspI CCGG 2 cut(s) 175, 225
MspR9I CCNGG 3 cut(s) 387, 504, 851
MvaI CCWGG 3 cut(s) 387, 504, 851
MwoI GCNNNNNNNGC 2 cut(s) 658, 980
NdeI CATATG 1 cut(s) 1117
NdeII GATC 2 cut(s) 496, 1108
NheI GCTAGC 1 cut(s) 86
NlaIII CATG 5 cut(s) 50, 110, 365, 640, 998
NlaIV GGNNCC 1 cut(s) 848
NmuCI GTSAC 5 cut(s) 291, 364, 709, 719, 763
NspI RCATGY 2 cut(s) 365, 998
NspV TTCGAA 1 cut(s) 880
PaeR7I CTCGAG 1 cut(s) 1130
PagI TCATGA 1 cut(s) 46
PciI ACATGT 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 1 cut(s) 465
PfoI TCCNGGA 1 cut(s) 385
PkrI GCNGC 2 cut(s) 589, 973
PleI GAGTC 4 cut(s) 103, 629, 740, 1093
PpsI GAGTC 4 cut(s) 103, 629, 740, 1093
PscI ACATGT 1 cut(s) 361
Psp6I CCWGG 3 cut(s) 385, 502, 849
PspGI CCWGG 3 cut(s) 385, 502, 849
PspN4I GGNNCC 1 cut(s) 848
PstNI CAGNNNCTG 1 cut(s) 902
RsaI GTAC 2 cut(s) 1019, 1114
RsaNI GTAC 2 cut(s) 1018, 1113
RseI CAYNNNNRTG 3 cut(s) 811, 822, 999
SalI GTCGAC 1 cut(s) 743
SaqAI TTAA 3 cut(s) 323, 908, 1159
SatI GCNGC 2 cut(s) 588, 972
Sau3AI GATC 2 cut(s) 496, 1108
SchI GAGTC 4 cut(s) 104, 630, 740, 1094
ScrFI CCNGG 3 cut(s) 387, 504, 851
SfaNI GCATC 4 cut(s) 91, 344, 499, 826
Sfr274I CTCGAG 1 cut(s) 1130
SfuI TTCGAA 1 cut(s) 880
SlaI CTCGAG 1 cut(s) 1130
SmiMI CAYNNNNRTG 3 cut(s) 811, 822, 999
SmlI CTYRAG 1 cut(s) 1130
SmoI CTYRAG 1 cut(s) 1130
Sse9I AATT 4 cut(s) 80, 202, 220, 936
SsiI CCGC 1 cut(s) 971
SspMI CTAG 4 cut(s) 87, 162, 183, 452
StyD4I CCNGG 3 cut(s) 385, 502, 849
StyI CCWWGG 5 cut(s) 130, 140, 469, 674, 919
TaaI ACNGT 2 cut(s) 715, 769
TaqI TCGA 5 cut(s) 521, 744, 880, 1038, 1131
TasI AATT 4 cut(s) 80, 202, 220, 936
TauI GCSGC 1 cut(s) 974
TfiI GAWTC 1 cut(s) 465
Tru1I TTAA 3 cut(s) 323, 908, 1159
Tru9I TTAA 3 cut(s) 323, 908, 1159
TscAI CASTG 4 cut(s) 441, 586, 687, 774
TseFI GTSAC 5 cut(s) 291, 364, 709, 719, 763
TseI GCWGC 1 cut(s) 587
Tsp45I GTSAC 5 cut(s) 291, 364, 709, 719, 763
TspDTI ATGAA 6 cut(s) 35, 45, 222, 299, 309, 1084
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 4 cut(s) 441, 586, 687, 774
XceI RCATGY 2 cut(s) 365, 998
XhoI CTCGAG 1 cut(s) 1130
XmiI GTMKAC 2 cut(s) 282, 744
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 4 cut(s) 87, 162, 183, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.