Rh5AG490800

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001622
Physical Location & Seq
Reverse (-)
83689093 .. 83691873
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG490800.1

Sequence Viewer

Length: 1194 bp
ATGGAAGATGTTCATGATGATGTGGATGGCGAACTTCGTGGTAGAGAATTGGCTAGCTTGGAGTCTGATGCTCATGAATCGGACTATGTGAATAGTGAAGTAGATGATACCACTGATGCTCAAGCAAGTAGAGCAGAAGACACCCAAGGTATTCCTTGGGATAGACTTAGCATTACTAGAGAGAAATACCGGCAAACTATGTTAGAACAATACAAGAATTATGAAAACATCCCTAATTCCGGAGAAGGGACAGGGAAGGACTGCAACATTAAAGAAAAAGGATCTTTATATTATGAGTTTCAATGCAACACAAGATCAGTGAAACCAACTATCCTTCATTTTCAGTTGTGTAACTTGGTCTGGGCTACATCAAAGCATGATGTCTACCTTATGTCACATTTTTCTGTCATTCATAAACATCCTGGAAGTCTCTTGGAAGGATTTACACAGACTCAAGTTAGTAGTCTTGCAGTAAAAGATAAGTTGCTAGTTGCCGGAGGATTCCAAGGAGAACTTATTTTCAAGCATCTTGATCAACCTGGAGTTAGCTTTTGTTCGAGGACAACTGATGATCATAATGCTATTGAGATTTATGACAATCCCAGTGGTGCAGCTCACTTTACAGCCTCAAGTAATGATTGTGGAGTCAGAGACTTTGATATGGAGACATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGCGAATCATACTTCTCTGAGTCCCGATGGTAAACTTTTTGTGATAGTTGGTGACAATCCAGATGGGATGTTGGTTGACTCTCAAACTGGCAAGACGGTGACGCCTTTATGTGGACACTTGGATTTCTCGTTTGCATCAGCGTGGCATCCTGATGGTGTCACCTTTGCTACTGGGAAGCAGGACAACACCTGTCGGGTTTGGGATGTTCGAAACCTATCCAAGTCAGTTGCTGTTTTAAAGGGAAACCTTGGAGCTATTCAGTCAATCCGTTATACTTCTGATGGTCAGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTACGAGAAGGAGCAGGAAATCGATTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCAGACACAGAGTCGCTTTTCATTGGAGTATGGGATCGTACATATGGTGGTCTTCTCGAGTATGGAAGACAAAAGTGTGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

397

Amino Acids

44.39

Weight (kDa)

4.92

Isoelectric Point (pI)

22.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 157 - 350 2.4e-14 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 205 - 353 7.4e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 206 - 396 1.1e-14 WDR5 beta-propeller domain
WD40_Gbeta PF25391 208 - 341 9.6e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 209 - 335 9e-06 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 240 - 350 1.7e-07 WDR55
WD40_CDC20-Fz PF24807 242 - 375 2.7e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 259 - 382 4.7e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 274 - 381 2.1e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 384
AccIII TCCGGA 1 cut(s) 239
AclWI GGATC 2 cut(s) 289, 1149
AcoI YGGCCR 1 cut(s) 701
AcyI GRCGYC 1 cut(s) 806
AfaI GTAC 2 cut(s) 1052, 1147
AfiI CCNNNNNNNGG 3 cut(s) 239, 246, 815
AgsI TTSAA 2 cut(s) 302, 523
AhdI GACNNNNNGTC 1 cut(s) 1117
AjnI CCWGG 2 cut(s) 421, 538
AluBI AGCT 5 cut(s) 57, 549, 614, 676, 959
AluI AGCT 5 cut(s) 57, 549, 614, 676, 959
Alw26I GTCTC 3 cut(s) 434, 645, 659
AlwI GGATC 2 cut(s) 289, 1149
AlwNI CAGNNNCTG 1 cut(s) 935
Ama87I CYCGRG 1 cut(s) 1163
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 1 cut(s) 701
ApeKI GCWGC 1 cut(s) 611
Asp700I GAANNNNTTC 1 cut(s) 9
AsuHPI GGTGA 4 cut(s) 767, 814, 856, 1094
AsuII TTCGAA 1 cut(s) 913
AsuNHI GCTAGC 1 cut(s) 53
AvaI CYCGRG 1 cut(s) 1163
BalI TGGCCA 1 cut(s) 703
BbsI GAAGAC 3 cut(s) 144, 1151, 1180
BbvI GCAGC 1 cut(s) 623
BccI CCATC 6 cut(s) 20, 725, 761, 851, 980, 997
BciT130I CCWGG 2 cut(s) 423, 540
BclI TGATCA 2 cut(s) 532, 571
BcoDI GTCTC 3 cut(s) 434, 645, 659
BfaI CTAG 3 cut(s) 54, 177, 488
BisI GCNGC 1 cut(s) 612
BlsI GCNGC 1 cut(s) 613
Bme1390I CCNGG 2 cut(s) 423, 540
BmeRI GACNNNNNGTC 1 cut(s) 1117
BmeT110I CYCGRG 1 cut(s) 1163
BmrFI CCNGG 2 cut(s) 423, 540
BmrI ACTGGG 2 cut(s) 597, 885
BmsI GCATC 5 cut(s) 58, 106, 535, 848, 859
BmtI GCTAGC 1 cut(s) 57
BmuI ACTGGG 2 cut(s) 597, 885
BpiI GAAGAC 3 cut(s) 144, 1151, 1180
BpmI CTGGAG 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 913
BpuEI CTTGAG 3 cut(s) 105, 438, 613
Bsa29I ATCGAT 1 cut(s) 1071
BsaHI GRCGYC 1 cut(s) 806
BsaJI CCNNGG 5 cut(s) 145, 155, 505, 698, 952
BsaWI WCCGGW 1 cut(s) 239
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 246, 815
Bse118I RCCGGY 1 cut(s) 189
Bse1I ACTGG 3 cut(s) 603, 796, 880
BseAI TCCGGA 1 cut(s) 239
BseBI CCWGG 2 cut(s) 423, 540
BseCI ATCGAT 1 cut(s) 1071
BseDI CCNNGG 5 cut(s) 145, 155, 505, 698, 952
BseGI GGATG 6 cut(s) 31, 228, 418, 777, 850, 913
BseLI CCNNNNNNNGG 3 cut(s) 239, 246, 815
BseMII CTCAG 1 cut(s) 713
BseNI ACTGG 3 cut(s) 603, 796, 880
BseXI GCAGC 1 cut(s) 623
BsgI GTGCAG 1 cut(s) 630
BshFI GGCC 1 cut(s) 703
BshVI ATCGAT 1 cut(s) 1071
BsiHKCI CYCGRG 1 cut(s) 1163
BsiSI CCGG 3 cut(s) 190, 240, 495
BslFI GGGAC 2 cut(s) 262, 711
BslI CCNNNNNNNGG 3 cut(s) 239, 246, 815
BsmAI GTCTC 3 cut(s) 434, 645, 659
BsmFI GGGAC 2 cut(s) 262, 711
BsnI GGCC 1 cut(s) 703
BsoBI CYCGRG 1 cut(s) 1163
Bsp119I TTCGAA 1 cut(s) 913
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 5 cut(s) 281, 314, 532, 571, 1141
BspANI GGCC 1 cut(s) 703
BspCNI CTCAG 1 cut(s) 714
BspDI ATCGAT 1 cut(s) 1071
BspEI TCCGGA 1 cut(s) 239
BspHI TCATGA 2 cut(s) 13, 73
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 2 cut(s) 289, 1149
BspT104I TTCGAA 1 cut(s) 913
BsrFI RCCGGY 1 cut(s) 189
BsrI ACTGG 3 cut(s) 603, 796, 880
BssAI RCCGGY 1 cut(s) 189
BssECI CCNNGG 5 cut(s) 145, 155, 505, 698, 952
BssMI GATC 5 cut(s) 281, 314, 532, 571, 1141
BssNI GRCGYC 1 cut(s) 806
BssT1I CCWWGG 5 cut(s) 145, 155, 505, 698, 952
Bst2UI CCWGG 2 cut(s) 423, 540
Bst4CI ACNGT 1 cut(s) 802
BstACI GRCGYC 1 cut(s) 806
BstBI TTCGAA 1 cut(s) 913
BstC8I GCNNGC 3 cut(s) 55, 705, 1014
BstDEI CTNAG 3 cut(s) 167, 681, 722
BstF5I GGATG 6 cut(s) 31, 228, 418, 777, 850, 913
BstKTI GATC 5 cut(s) 284, 317, 535, 574, 1144
BstMAI GTCTC 3 cut(s) 434, 645, 659
BstMBI GATC 5 cut(s) 281, 314, 532, 571, 1141
BstMWI GCNNNNNNNGC 3 cut(s) 131, 682, 1013
BstNI CCWGG 2 cut(s) 423, 540
BstNSI RCATGY 1 cut(s) 1031
BstSCI CCNGG 2 cut(s) 421, 538
BstV1I GCAGC 1 cut(s) 623
BstV2I GAAGAC 3 cut(s) 144, 1151, 1180
BstX2I RGATCY 1 cut(s) 281
BstYI RGATCY 1 cut(s) 281
Bsu15I ATCGAT 1 cut(s) 1071
BsuRI GGCC 1 cut(s) 703
BsuTUI ATCGAT 1 cut(s) 1071
BtsCI GGATG 6 cut(s) 31, 228, 418, 777, 850, 913
BtsIMutI CAGTG 3 cut(s) 111, 324, 610
Cac8I GCNNGC 3 cut(s) 55, 705, 1014
CaiI CAGNNNCTG 1 cut(s) 935
CciI TCATGA 2 cut(s) 13, 73
Cfr10I RCCGGY 1 cut(s) 189
ClaI ATCGAT 1 cut(s) 1071
CseI GACGC 1 cut(s) 814
Csp6I GTAC 2 cut(s) 1051, 1146
CspCI CAANNNNNGTGG 2 cut(s) 586, 621
CviAII CATG 4 cut(s) 14, 74, 377, 1028
CviQI GTAC 2 cut(s) 1051, 1146
DdeI CTNAG 3 cut(s) 167, 681, 722
DpnI GATC 5 cut(s) 283, 316, 534, 573, 1143
DpnII GATC 5 cut(s) 281, 314, 532, 571, 1141
DraI TTTAAA 1 cut(s) 942
DriI GACNNNNNGTC 1 cut(s) 1117
EaeI YGGCCR 1 cut(s) 701
Eam1105I GACNNNNNGTC 1 cut(s) 1117
Eco130I CCWWGG 5 cut(s) 145, 155, 505, 698, 952
Eco32I GATATC 1 cut(s) 1089
Eco88I CYCGRG 1 cut(s) 1163
EcoRII CCWGG 2 cut(s) 421, 538
EcoRV GATATC 1 cut(s) 1089
EcoT14I CCWWGG 5 cut(s) 145, 155, 505, 698, 952
ErhI CCWWGG 5 cut(s) 145, 155, 505, 698, 952
FaeI CATG 4 cut(s) 17, 77, 380, 1031
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 2 cut(s) 262, 711
FatI CATG 4 cut(s) 13, 73, 376, 1027
FauNDI CATATG 1 cut(s) 1150
FbaI TGATCA 2 cut(s) 532, 571
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 1 cut(s) 612
FokI GGATG 6 cut(s) 38, 215, 405, 784, 837, 920
Fsp4HI GCNGC 1 cut(s) 612
FspBI CTAG 3 cut(s) 54, 177, 488
GluI GCNGC 1 cut(s) 612
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 1 cut(s) 703
HapII CCGG 3 cut(s) 190, 240, 495
HgaI GACGC 1 cut(s) 814
Hin1I GRCGYC 1 cut(s) 806
Hin1II CATG 4 cut(s) 17, 77, 380, 1031
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HinfI GANTC 9 cut(s) 62, 77, 451, 501, 645, 709, 724, 782, 1118
HpaII CCGG 3 cut(s) 190, 240, 495
HphI GGTGA 4 cut(s) 767, 814, 856, 1094
Hpy166II GTNNAC 4 cut(s) 385, 737, 781, 818
Hpy188I TCNGA 5 cut(s) 67, 82, 650, 723, 985
Hpy188III TCNNGA 9 cut(s) 14, 74, 240, 530, 728, 764, 854, 1109, 1163
Hpy8I GTNNAC 4 cut(s) 385, 737, 781, 818
HpyAV CCTTC 5 cut(s) 239, 250, 344, 431, 1051
HpyCH4III ACNGT 1 cut(s) 802
HpyCH4V TGCA 6 cut(s) 264, 306, 470, 611, 839, 1027
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 682, 1013
HpyF3I CTNAG 3 cut(s) 167, 681, 722
Hsp92I GRCGYC 1 cut(s) 806
Hsp92II CATG 4 cut(s) 17, 77, 380, 1031
Kpn2I TCCGGA 1 cut(s) 239
Ksp22I TGATCA 2 cut(s) 532, 571
Kzo9I GATC 5 cut(s) 281, 314, 532, 571, 1141
LmnI GCTCC 2 cut(s) 956, 1060
Lsp1109I GCAGC 1 cut(s) 623
LweI GCATC 5 cut(s) 58, 106, 535, 848, 859
MaeI CTAG 3 cut(s) 54, 177, 488
MaeIII GTNAC 5 cut(s) 350, 393, 755, 802, 862
MalI GATC 5 cut(s) 283, 316, 534, 573, 1143
MboI GATC 5 cut(s) 281, 314, 532, 571, 1141
MboII GAAGA 4 cut(s) 17, 149, 1151, 1185
MflI RGATCY 1 cut(s) 281
MlsI TGGCCA 1 cut(s) 703
MluCI AATT 3 cut(s) 47, 217, 235
MluNI TGGCCA 1 cut(s) 703
MlyI GAGTC 6 cut(s) 71, 445, 654, 733, 776, 1127
MnlI CCTC 3 cut(s) 491, 552, 637
Mox20I TGGCCA 1 cut(s) 703
MroI TCCGGA 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 9
MscI TGGCCA 1 cut(s) 703
MseI TTAA 3 cut(s) 270, 941, 1192
MslI CAYNNNNRTG 4 cut(s) 18, 844, 855, 1032
Msp20I TGGCCA 1 cut(s) 703
MspI CCGG 3 cut(s) 190, 240, 495
MspR9I CCNGG 2 cut(s) 423, 540
MvaI CCWGG 2 cut(s) 423, 540
MwoI GCNNNNNNNGC 3 cut(s) 131, 682, 1013
NdeI CATATG 1 cut(s) 1150
NdeII GATC 5 cut(s) 281, 314, 532, 571, 1141
NheI GCTAGC 1 cut(s) 53
NlaIII CATG 4 cut(s) 17, 77, 380, 1031
NmuCI GTSAC 4 cut(s) 393, 755, 802, 862
NspI RCATGY 1 cut(s) 1031
NspV TTCGAA 1 cut(s) 913
PaeR7I CTCGAG 1 cut(s) 1163
PagI TCATGA 2 cut(s) 13, 73
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 77, 501, 709
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 1 cut(s) 613
PleI GAGTC 6 cut(s) 70, 445, 653, 732, 776, 1126
PpsI GAGTC 6 cut(s) 70, 445, 653, 732, 776, 1126
Psp6I CCWGG 2 cut(s) 421, 538
PspGI CCWGG 2 cut(s) 421, 538
PstNI CAGNNNCTG 1 cut(s) 935
PsuI RGATCY 1 cut(s) 281
RsaI GTAC 2 cut(s) 1052, 1147
RsaNI GTAC 2 cut(s) 1051, 1146
RseI CAYNNNNRTG 4 cut(s) 18, 844, 855, 1032
SaqAI TTAA 3 cut(s) 270, 941, 1192
SatI GCNGC 1 cut(s) 612
Sau3AI GATC 5 cut(s) 281, 314, 532, 571, 1141
SchI GAGTC 6 cut(s) 71, 445, 654, 733, 776, 1127
ScrFI CCNGG 2 cut(s) 423, 540
SfaNI GCATC 5 cut(s) 58, 106, 535, 848, 859
Sfr274I CTCGAG 1 cut(s) 1163
SfuI TTCGAA 1 cut(s) 913
SlaI CTCGAG 1 cut(s) 1163
SmiMI CAYNNNNRTG 4 cut(s) 18, 844, 855, 1032
SmlI CTYRAG 4 cut(s) 120, 453, 628, 1163
SmoI CTYRAG 4 cut(s) 120, 453, 628, 1163
Sse9I AATT 3 cut(s) 47, 217, 235
SspMI CTAG 3 cut(s) 54, 177, 488
StyD4I CCNGG 2 cut(s) 421, 538
StyI CCWWGG 5 cut(s) 145, 155, 505, 698, 952
TaaI ACNGT 1 cut(s) 802
TaqI TCGA 4 cut(s) 557, 913, 1071, 1164
TasI AATT 3 cut(s) 47, 217, 235
TfiI GAWTC 3 cut(s) 77, 501, 709
Tru1I TTAA 3 cut(s) 270, 941, 1192
Tru9I TTAA 3 cut(s) 270, 941, 1192
TscAI CASTG 3 cut(s) 118, 324, 610
TseFI GTSAC 4 cut(s) 393, 755, 802, 862
TseI GCWGC 1 cut(s) 611
Tsp45I GTSAC 4 cut(s) 393, 755, 802, 862
TspDTI ATGAA 5 cut(s) 90, 237, 326, 401, 1117
TspGWI ACGGA 2 cut(s) 680, 962
TspRI CASTG 3 cut(s) 118, 324, 610
XceI RCATGY 1 cut(s) 1031
XhoI CTCGAG 1 cut(s) 1163
XmiI GTMKAC 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 3 cut(s) 54, 177, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.