FvH4_3g41640

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001622
Physical Location & Seq
Reverse (-)
34950366 .. 34951635
1270 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41640.t1

Sequence Viewer

Length: 621 bp
ATGCCATTACGAATGCTGTTGAGATTTATGACAGTCCCAGTTCACTTTACAGCATCCAATAATGATTGTGGAGTCAGGGACTTCGACATGGAGAAATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGTTAATCATACTTCTTTGAGTCCCAATGGCAAACTTCTTGTGATTGTTGGTGACAATCCGGATGGGATGTTGGTGGATTCTCAGACTGGAAAGACTGTGACGTCTTTATCTGGGCACTTGGATTTCTCGTTTGCATCAGCCTGGCATCCTGATGGTGTTACCTTTGCTACTGGGAACCAGGACAAAACTTGTCGGGTTTGGGATGTTCGAAACCTATCCAAGTCAGTTGCTGTTCTAAAGGGAAATCTTGGAGCTATTCGGTCAATTCGTTATACTTCTGATGGACGGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTATGAGAAGGAGCAGGAAATCGACTTCTTTGGTGAGATATCTGGTATATCATTCAGTCCAGACACGGAGTCGCTTTTCATTGGAGTGTGGGATCGTACGTATGGTAGTCTTCTCGAGTATGGACGCCTAAAGTGTGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

207

Amino Acids

23.11

Weight (kDa)

6.05

Isoelectric Point (pI)

22.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML_2 PF23414 16 - 161 3.2e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 17 - 192 1.1e-15 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 18 - 114 9.2e-07 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 21 - 156 1.1e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 23 - 118 4.7e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 43 - 159 1.1e-15 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 45 - 191 4.3e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 47 - 184 2e-09 CDC20/Fizzy WD40 domain
WDR55 PF24796 47 - 159 4.1e-09 WDR55
WD40 PF00400 74 - 112 1.1e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 91 - 192 9.1e-14 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 232
AatII GACGTC 1 cut(s) 236
AccIII TCCGGA 1 cut(s) 190
AclWI GGATC 1 cut(s) 576
AcoI YGGCCR 1 cut(s) 128
AcsI RAATTY 1 cut(s) 95
AcyI GRCGYC 2 cut(s) 233, 601
AfaI GTAC 1 cut(s) 574
AhdI GACNNNNNGTC 1 cut(s) 544
AjnI CCWGG 2 cut(s) 272, 309
AluBI AGCT 2 cut(s) 103, 386
AluI AGCT 2 cut(s) 103, 386
AlwI GGATC 1 cut(s) 576
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 590
Aor13HI TCCGGA 1 cut(s) 190
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 1 cut(s) 614
ApoI RAATTY 1 cut(s) 95
AsuHPI GGTGA 2 cut(s) 194, 521
AsuII TTCGAA 1 cut(s) 340
AvaI CYCGRG 1 cut(s) 590
BaeGI GKGCMC 1 cut(s) 249
BalI TGGCCA 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 578
BbvI GCAGC 1 cut(s) 601
BccI CCATC 4 cut(s) 188, 278, 407, 424
BciT130I CCWGG 2 cut(s) 274, 311
BisI GCNGC 1 cut(s) 615
BlsI GCNGC 1 cut(s) 616
Bme1390I CCNGG 2 cut(s) 274, 311
BmeRI GACNNNNNGTC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 590
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 2 cut(s) 274, 311
BmrI ACTGGG 2 cut(s) 32, 312
BmsI GCATC 3 cut(s) 62, 275, 286
BmuI ACTGGG 2 cut(s) 32, 312
BpiI GAAGAC 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 340
BsaAI YACGTR 1 cut(s) 576
BsaHI GRCGYC 2 cut(s) 233, 601
BsaJI CCNNGG 1 cut(s) 125
BsaWI WCCGGW 1 cut(s) 190
Bse1I ACTGG 4 cut(s) 38, 131, 223, 307
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 2 cut(s) 274, 311
BseDI CCNNGG 1 cut(s) 125
BseGI GGATG 5 cut(s) 53, 199, 204, 277, 340
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 4 cut(s) 38, 131, 223, 307
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 1 cut(s) 601
BshFI GGCC 1 cut(s) 130
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 1 cut(s) 191
BsiWI CGTACG 1 cut(s) 572
BslFI GGGAC 3 cut(s) 20, 92, 138
BsmFI GGGAC 3 cut(s) 20, 92, 138
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 590
Bsp119I TTCGAA 1 cut(s) 340
Bsp1286I GDGCHC 1 cut(s) 249
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 1 cut(s) 568
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 1 cut(s) 226
BspEI TCCGGA 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 308
BspPI GGATC 1 cut(s) 576
BspT104I TTCGAA 1 cut(s) 340
BsrI ACTGG 4 cut(s) 38, 131, 223, 307
BssECI CCNNGG 1 cut(s) 125
BssMI GATC 1 cut(s) 568
BssNI GRCGYC 2 cut(s) 233, 601
BssT1I CCWWGG 1 cut(s) 125
Bst2UI CCWGG 2 cut(s) 274, 311
Bst4CI ACNGT 3 cut(s) 34, 229, 420
BstACI GRCGYC 2 cut(s) 233, 601
BstBAI YACGTR 1 cut(s) 576
BstBI TTCGAA 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 2 cut(s) 108, 213
BstF5I GGATG 5 cut(s) 53, 199, 204, 277, 340
BstKTI GATC 1 cut(s) 571
BstMBI GATC 1 cut(s) 568
BstMWI GCNNNNNNNGC 2 cut(s) 109, 440
BstNI CCWGG 2 cut(s) 274, 311
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 272, 309
BstSLI GKGCMC 1 cut(s) 249
BstSNI TACGTA 1 cut(s) 576
BstV1I GCAGC 1 cut(s) 601
BstV2I GAAGAC 1 cut(s) 578
BsuRI GGCC 1 cut(s) 130
BtsCI GGATG 5 cut(s) 53, 199, 204, 277, 340
Cac8I GCNNGC 1 cut(s) 441
CaiI CAGNNNCTG 1 cut(s) 362
CseI GACGC 1 cut(s) 609
Csp6I GTAC 1 cut(s) 573
CviAII CATG 2 cut(s) 88, 455
CviJI RGCY 5 cut(s) 103, 130, 272, 386, 439
CviKI_1 RGCY 5 cut(s) 103, 130, 272, 386, 439
CviQI GTAC 1 cut(s) 573
DdeI CTNAG 2 cut(s) 108, 213
DpnI GATC 1 cut(s) 570
DpnII GATC 1 cut(s) 568
DrdI GACNNNNNNGTC 1 cut(s) 232
DriI GACNNNNNGTC 1 cut(s) 544
DseDI GACNNNNNNGTC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 128
Eam1105I GACNNNNNGTC 1 cut(s) 544
Eco105I TACGTA 1 cut(s) 576
Eco130I CCWWGG 1 cut(s) 125
Eco32I GATATC 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 272, 309
EcoRV GATATC 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 125
FaeI CATG 2 cut(s) 91, 458
FaqI GGGAC 3 cut(s) 20, 92, 138
FatI CATG 2 cut(s) 87, 454
Fnu4HI GCNGC 1 cut(s) 615
FokI GGATG 5 cut(s) 40, 206, 211, 264, 347
Fsp4HI GCNGC 1 cut(s) 615
GluI GCNGC 1 cut(s) 615
HaeIII GGCC 1 cut(s) 130
HapII CCGG 1 cut(s) 191
HgaI GACGC 1 cut(s) 609
Hin1I GRCGYC 2 cut(s) 233, 601
Hin1II CATG 2 cut(s) 91, 458
HinfI GANTC 4 cut(s) 72, 151, 209, 545
HpaII CCGG 1 cut(s) 191
HphI GGTGA 2 cut(s) 194, 521
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 216, 412
Hpy188III TCNNGA 4 cut(s) 191, 281, 536, 590
Hpy8I GTNNAC 1 cut(s) 43
HpyAV CCTTC 1 cut(s) 478
HpyCH4III ACNGT 3 cut(s) 34, 229, 420
HpyCH4IV ACGT 2 cut(s) 233, 575
HpyCH4V TGCA 2 cut(s) 266, 454
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 440
HpyF3I CTNAG 2 cut(s) 108, 213
HpySE526I ACGT 2 cut(s) 233, 575
Hsp92I GRCGYC 2 cut(s) 233, 601
Hsp92II CATG 2 cut(s) 91, 458
Kpn2I TCCGGA 1 cut(s) 190
Kzo9I GATC 1 cut(s) 568
LmnI GCTCC 2 cut(s) 383, 487
Lsp1109I GCAGC 1 cut(s) 601
LweI GCATC 3 cut(s) 62, 275, 286
MaeII ACGT 2 cut(s) 233, 575
MaeIII GTNAC 3 cut(s) 182, 229, 289
MalI GATC 1 cut(s) 570
MboI GATC 1 cut(s) 568
MboII GAAGA 1 cut(s) 578
MhlI GDGCHC 1 cut(s) 249
MlsI TGGCCA 1 cut(s) 130
MluCI AATT 2 cut(s) 95, 396
MluNI TGGCCA 1 cut(s) 130
MlyI GAGTC 3 cut(s) 81, 160, 554
Mox20I TGGCCA 1 cut(s) 130
MroI TCCGGA 1 cut(s) 190
MscI TGGCCA 1 cut(s) 130
MseI TTAA 2 cut(s) 135, 619
MslI CAYNNNNRTG 3 cut(s) 282, 459, 560
Msp20I TGGCCA 1 cut(s) 130
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 2 cut(s) 274, 311
Mva1269I GAATGC 1 cut(s) 18
MvaI CCWGG 2 cut(s) 274, 311
MwoI GCNNNNNNNGC 2 cut(s) 109, 440
NdeII GATC 1 cut(s) 568
NlaIII CATG 2 cut(s) 91, 458
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 2 cut(s) 182, 229
NspI RCATGY 1 cut(s) 458
NspV TTCGAA 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 590
PcsI WCGNNNNNNNCGW 1 cut(s) 397
PctI GAATGC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 209
Pfl23II CGTACG 1 cut(s) 572
PkrI GCNGC 1 cut(s) 616
PleI GAGTC 3 cut(s) 80, 159, 553
PpsI GAGTC 3 cut(s) 80, 159, 553
Ppu21I YACGTR 1 cut(s) 576
Psp6I CCWGG 2 cut(s) 272, 309
PspGI CCWGG 2 cut(s) 272, 309
PspLI CGTACG 1 cut(s) 572
PspN4I GGNNCC 1 cut(s) 308
PstNI CAGNNNCTG 1 cut(s) 362
RsaI GTAC 1 cut(s) 574
RsaNI GTAC 1 cut(s) 573
RseI CAYNNNNRTG 3 cut(s) 282, 459, 560
SaqAI TTAA 2 cut(s) 135, 619
SatI GCNGC 1 cut(s) 615
Sau3AI GATC 1 cut(s) 568
SchI GAGTC 3 cut(s) 81, 160, 554
ScrFI CCNGG 2 cut(s) 274, 311
SduI GDGCHC 1 cut(s) 249
SetI ASST 6 cut(s) 105, 236, 296, 348, 388, 578
SfaNI GCATC 3 cut(s) 62, 275, 286
Sfr274I CTCGAG 1 cut(s) 590
SfuI TTCGAA 1 cut(s) 340
SlaI CTCGAG 1 cut(s) 590
SmiMI CAYNNNNRTG 3 cut(s) 282, 459, 560
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
SnaBI TACGTA 1 cut(s) 576
Sse9I AATT 2 cut(s) 95, 396
StyD4I CCNGG 2 cut(s) 272, 309
StyI CCWWGG 1 cut(s) 125
TaaI ACNGT 3 cut(s) 34, 229, 420
TaiI ACGT 2 cut(s) 236, 578
TaqI TCGA 4 cut(s) 84, 340, 498, 591
TaqII GACCGA 1 cut(s) 381
TasI AATT 2 cut(s) 95, 396
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 2 cut(s) 135, 619
Tru9I TTAA 2 cut(s) 135, 619
TseFI GTSAC 2 cut(s) 182, 229
TseI GCWGC 1 cut(s) 614
Tsp45I GTSAC 2 cut(s) 182, 229
TspDTI ATGAA 1 cut(s) 544
TspGWI ACGGA 2 cut(s) 107, 557
XapI RAATTY 1 cut(s) 95
XceI RCATGY 1 cut(s) 458
XhoI CTCGAG 1 cut(s) 590
ZraI GACGTC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.