Rroxscaffold_1G00006840

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001622
Physical Location & Seq
Forward (+)
8837453 .. 8842567
5115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006840.1

Sequence Viewer

Length: 1017 bp
ATGGAAGATGTTCATGATGATGTGGGTGGCGAACTTCGTGATAGAGAATTGGCTAGCTTGGAGTCTGATGCTCATGAATCGGACTATGTGTTGCGTAACTTGGTCTGGGCTACATCAAAGCATGATGTGTACCTTCTGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCGAAGCATCTGAGGTTCTAAATGTTTCAGGACATGTCACACCATCAGAGAAACATCCTGGAAGTCTCTTGGAGGGATTTACACGGACTCAAGTTAGTAGTCTTGCAGTGAAAGATAAGTTGCTAGTTTCTGGAGGATTCCAAGGAGAACTTATTTGCAAGCATCTTGATCAACCTGGAGTTAGCTTTTGTTCGAGGACAACTGATGATGATAATGCCATTACAAATGCTATTGAGATTTATGACAATCCCAGTGGTGCAGCTCACTTTACAGCCTCAAGTAATGATTGTGGAGTCAGAGACTTTGACACGGAGACATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGCGAATCATACTTCTCTGAGTCCCGATGGTAAACTTCTTGTGATAGTTGGTGACAATCCAGATGGGATGTTGGTCGACTCTCAAACTGGCAAGACGGTGACGCCTTTATGTGGACACTTGGATTTCTTGTTTGCATCAGCGTGGCATCCTGATGGTGTCACCTTTGCTACTGGGAACCAGGACAAAACCTGTCGCGTTTGGGATGTTCGAAACCTATCCAAGTCAGTTGCTGTTTTAAAGGGAAACCTTGGAGCTATTCGGTCAATCCGTTATACTTCTGATGGTCAGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTACGAGAAGGAGCAGGAAATCGATTTATTTGGTGAGATATCTGGCATATCATTCAGTCCAGACACAGAGTCGCTTTTCATTGGAGTATGGGATCGTACATATGGTAGTCTTCTCGAGTATGGAAGACAAAAGTGTGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

338

Amino Acids

37.25

Weight (kDa)

4.88

Isoelectric Point (pI)

23.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 91 - 291 1.4e-14 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 146 - 294 4.3e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 147 - 337 2.8e-16 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 149 - 316 7.1e-19 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 150 - 249 9.7e-06 WDHD1 first WD40 domain
WD40_Gbeta PF25391 150 - 287 1.9e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 150 - 246 3.1e-06 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 181 - 291 2.5e-07 WDR55
WD40_CDC20-Fz PF24807 182 - 316 3.5e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 200 - 323 6.5e-10 WDR3 first beta-propeller domain
WD40 PF00400 207 - 244 4.6e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 221 - 323 1e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 603
AccII CGCG 1 cut(s) 723
AclWI GGATC 1 cut(s) 972
AcoI YGGCCR 1 cut(s) 524
AcyI GRCGYC 1 cut(s) 629
AfaI GTAC 3 cut(s) 131, 875, 970
AfiI CCNNNNNNNGG 1 cut(s) 638
AflIII ACRYGT 1 cut(s) 208
AhdI GACNNNNNGTC 1 cut(s) 940
AjnI CCWGG 3 cut(s) 232, 349, 705
AluBI AGCT 5 cut(s) 57, 360, 437, 499, 782
AluI AGCT 5 cut(s) 57, 360, 437, 499, 782
Alw26I GTCTC 3 cut(s) 245, 468, 482
AlwI GGATC 1 cut(s) 972
AlwNI CAGNNNCTG 1 cut(s) 758
Ama87I CYCGRG 1 cut(s) 986
AoxI GGCC 1 cut(s) 524
ApeKI GCWGC 1 cut(s) 434
Asp700I GAANNNNTTC 1 cut(s) 9
AsuHPI GGTGA 4 cut(s) 590, 637, 679, 917
AsuII TTCGAA 1 cut(s) 736
AsuNHI GCTAGC 1 cut(s) 53
AvaI CYCGRG 1 cut(s) 986
BalI TGGCCA 1 cut(s) 526
BbsI GAAGAC 3 cut(s) 156, 974, 1003
BbvI GCAGC 1 cut(s) 446
BccI CCATC 6 cut(s) 226, 548, 584, 674, 803, 820
BciT130I CCWGG 3 cut(s) 234, 351, 707
BclI TGATCA 1 cut(s) 343
BcoDI GTCTC 3 cut(s) 245, 468, 482
BfaI CTAG 2 cut(s) 54, 299
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
Bme1390I CCNGG 3 cut(s) 234, 351, 707
BmeRI GACNNNNNGTC 1 cut(s) 940
BmeT110I CYCGRG 1 cut(s) 986
BmiI GGNNCC 1 cut(s) 704
BmrFI CCNGG 3 cut(s) 234, 351, 707
BmrI ACTGGG 2 cut(s) 420, 708
BmsI GCATC 5 cut(s) 58, 191, 346, 671, 682
BmtI GCTAGC 1 cut(s) 57
BmuI ACTGGG 2 cut(s) 420, 708
BpiI GAAGAC 3 cut(s) 156, 974, 1003
BpmI CTGGAG 2 cut(s) 327, 372
Bpu14I TTCGAA 1 cut(s) 736
BpuEI CTTGAG 2 cut(s) 249, 436
Bsa29I ATCGAT 1 cut(s) 894
BsaHI GRCGYC 1 cut(s) 629
BsaJI CCNNGG 3 cut(s) 316, 521, 775
Bsc4I CCNNNNNNNGG 1 cut(s) 638
Bse1I ACTGG 3 cut(s) 426, 619, 703
BseBI CCWGG 3 cut(s) 234, 351, 707
BseCI ATCGAT 1 cut(s) 894
BseDI CCNNGG 3 cut(s) 316, 521, 775
BseGI GGATG 4 cut(s) 229, 600, 673, 736
BseLI CCNNNNNNNGG 1 cut(s) 638
BseMII CTCAG 2 cut(s) 177, 536
BseNI ACTGG 3 cut(s) 426, 619, 703
BseXI GCAGC 1 cut(s) 446
BsgI GTGCAG 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 723
BshFI GGCC 1 cut(s) 526
BshVI ATCGAT 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 986
BslFI GGGAC 1 cut(s) 534
BslI CCNNNNNNNGG 1 cut(s) 638
BsmAI GTCTC 3 cut(s) 245, 468, 482
BsmFI GGGAC 1 cut(s) 534
BsnI GGCC 1 cut(s) 526
BsoBI CYCGRG 1 cut(s) 986
Bsp119I TTCGAA 1 cut(s) 736
Bsp143I GATC 2 cut(s) 343, 964
BspANI GGCC 1 cut(s) 526
BspCNI CTCAG 2 cut(s) 178, 537
BspDI ATCGAT 1 cut(s) 894
BspFNI CGCG 1 cut(s) 723
BspHI TCATGA 2 cut(s) 13, 73
BspLI GGNNCC 1 cut(s) 704
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 1 cut(s) 972
BspT104I TTCGAA 1 cut(s) 736
BsrI ACTGG 3 cut(s) 426, 619, 703
BssECI CCNNGG 3 cut(s) 316, 521, 775
BssMI GATC 2 cut(s) 343, 964
BssNI GRCGYC 1 cut(s) 629
BssT1I CCWWGG 3 cut(s) 316, 521, 775
Bst2UI CCWGG 3 cut(s) 234, 351, 707
Bst4CI ACNGT 1 cut(s) 625
BstACI GRCGYC 1 cut(s) 629
BstBI TTCGAA 1 cut(s) 736
BstC8I GCNNGC 4 cut(s) 55, 335, 528, 837
BstDEI CTNAG 3 cut(s) 186, 504, 545
BstF5I GGATG 4 cut(s) 229, 600, 673, 736
BstFNI CGCG 1 cut(s) 723
BstKTI GATC 2 cut(s) 346, 967
BstMAI GTCTC 3 cut(s) 245, 468, 482
BstMBI GATC 2 cut(s) 343, 964
BstMWI GCNNNNNNNGC 2 cut(s) 505, 836
BstNI CCWGG 3 cut(s) 234, 351, 707
BstNSI RCATGY 2 cut(s) 212, 854
BstSCI CCNGG 3 cut(s) 232, 349, 705
BstUI CGCG 1 cut(s) 723
BstV1I GCAGC 1 cut(s) 446
BstV2I GAAGAC 3 cut(s) 156, 974, 1003
Bsu15I ATCGAT 1 cut(s) 894
BsuRI GGCC 1 cut(s) 526
BsuTUI ATCGAT 1 cut(s) 894
BtsCI GGATG 4 cut(s) 229, 600, 673, 736
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 2 cut(s) 288, 433
Cac8I GCNNGC 4 cut(s) 55, 335, 528, 837
CaiI CAGNNNCTG 1 cut(s) 758
CciI TCATGA 2 cut(s) 13, 73
ClaI ATCGAT 1 cut(s) 894
CseI GACGC 1 cut(s) 637
Csp6I GTAC 3 cut(s) 130, 874, 969
CspCI CAANNNNNGTGG 2 cut(s) 409, 444
CviAII CATG 5 cut(s) 14, 74, 122, 209, 851
CviQI GTAC 3 cut(s) 130, 874, 969
DdeI CTNAG 3 cut(s) 186, 504, 545
DpnI GATC 2 cut(s) 345, 966
DpnII GATC 2 cut(s) 343, 964
DraI TTTAAA 1 cut(s) 765
DriI GACNNNNNGTC 1 cut(s) 940
EaeI YGGCCR 1 cut(s) 524
Eam1105I GACNNNNNGTC 1 cut(s) 940
Eco130I CCWWGG 3 cut(s) 316, 521, 775
Eco32I GATATC 1 cut(s) 912
Eco88I CYCGRG 1 cut(s) 986
EcoRII CCWGG 3 cut(s) 232, 349, 705
EcoRV GATATC 1 cut(s) 912
EcoT14I CCWWGG 3 cut(s) 316, 521, 775
ErhI CCWWGG 3 cut(s) 316, 521, 775
FaeI CATG 5 cut(s) 17, 77, 125, 212, 854
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 534
FatI CATG 5 cut(s) 13, 73, 121, 208, 850
FauNDI CATATG 1 cut(s) 973
FbaI TGATCA 1 cut(s) 343
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 4 cut(s) 216, 607, 660, 743
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 2 cut(s) 54, 299
GluI GCNGC 1 cut(s) 435
GsuI CTGGAG 2 cut(s) 327, 372
HaeIII GGCC 1 cut(s) 526
HgaI GACGC 1 cut(s) 637
Hin1I GRCGYC 1 cut(s) 629
Hin1II CATG 5 cut(s) 17, 77, 125, 212, 854
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HinfI GANTC 9 cut(s) 62, 77, 262, 312, 468, 532, 547, 605, 941
HphI GGTGA 4 cut(s) 590, 637, 679, 917
Hpy166II GTNNAC 4 cut(s) 130, 560, 604, 641
Hpy188I TCNGA 7 cut(s) 67, 82, 187, 223, 473, 546, 808
Hpy8I GTNNAC 4 cut(s) 130, 560, 604, 641
HpyAV CCTTC 2 cut(s) 143, 874
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4V TGCA 5 cut(s) 281, 333, 434, 662, 850
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 836
HpyF3I CTNAG 3 cut(s) 186, 504, 545
Hsp92I GRCGYC 1 cut(s) 629
Hsp92II CATG 5 cut(s) 17, 77, 125, 212, 854
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 2 cut(s) 343, 964
LmnI GCTCC 2 cut(s) 779, 883
Lsp1109I GCAGC 1 cut(s) 446
LweI GCATC 5 cut(s) 58, 191, 346, 671, 682
MaeI CTAG 2 cut(s) 54, 299
MaeIII GTNAC 6 cut(s) 95, 138, 211, 578, 625, 685
MalI GATC 2 cut(s) 345, 966
MboI GATC 2 cut(s) 343, 964
MboII GAAGA 4 cut(s) 17, 156, 974, 1008
MlsI TGGCCA 1 cut(s) 526
MluCI AATT 1 cut(s) 47
MluNI TGGCCA 1 cut(s) 526
MlyI GAGTC 6 cut(s) 71, 256, 477, 556, 599, 950
MnlI CCTC 5 cut(s) 181, 241, 302, 363, 460
Mox20I TGGCCA 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 9
MscI TGGCCA 1 cut(s) 526
MseI TTAA 3 cut(s) 170, 764, 1015
MslI CAYNNNNRTG 4 cut(s) 18, 667, 678, 855
Msp20I TGGCCA 1 cut(s) 526
MspR9I CCNGG 3 cut(s) 234, 351, 707
MvaI CCWGG 3 cut(s) 234, 351, 707
MvnI CGCG 1 cut(s) 723
MwoI GCNNNNNNNGC 2 cut(s) 505, 836
NdeI CATATG 1 cut(s) 973
NdeII GATC 2 cut(s) 343, 964
NheI GCTAGC 1 cut(s) 53
NlaIII CATG 5 cut(s) 17, 77, 125, 212, 854
NlaIV GGNNCC 1 cut(s) 704
NmuCI GTSAC 5 cut(s) 138, 211, 578, 625, 685
NspI RCATGY 2 cut(s) 212, 854
NspV TTCGAA 1 cut(s) 736
PaeR7I CTCGAG 1 cut(s) 986
PagI TCATGA 2 cut(s) 13, 73
PciI ACATGT 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 793
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 77, 312, 532
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 1 cut(s) 436
PleI GAGTC 6 cut(s) 70, 256, 476, 555, 599, 949
PpsI GAGTC 6 cut(s) 70, 256, 476, 555, 599, 949
PscI ACATGT 1 cut(s) 208
Psp6I CCWGG 3 cut(s) 232, 349, 705
PspGI CCWGG 3 cut(s) 232, 349, 705
PspN4I GGNNCC 1 cut(s) 704
PstNI CAGNNNCTG 1 cut(s) 758
RsaI GTAC 3 cut(s) 131, 875, 970
RsaNI GTAC 3 cut(s) 130, 874, 969
RseI CAYNNNNRTG 4 cut(s) 18, 667, 678, 855
SalI GTCGAC 1 cut(s) 602
SaqAI TTAA 3 cut(s) 170, 764, 1015
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 2 cut(s) 343, 964
SchI GAGTC 6 cut(s) 71, 256, 477, 556, 599, 950
ScrFI CCNGG 3 cut(s) 234, 351, 707
SfaNI GCATC 5 cut(s) 58, 191, 346, 671, 682
Sfr274I CTCGAG 1 cut(s) 986
SfuI TTCGAA 1 cut(s) 736
SlaI CTCGAG 1 cut(s) 986
SmiMI CAYNNNNRTG 4 cut(s) 18, 667, 678, 855
SmlI CTYRAG 3 cut(s) 264, 451, 986
SmoI CTYRAG 3 cut(s) 264, 451, 986
Sse9I AATT 1 cut(s) 47
SspMI CTAG 2 cut(s) 54, 299
StyD4I CCNGG 3 cut(s) 232, 349, 705
StyI CCWWGG 3 cut(s) 316, 521, 775
TaaI ACNGT 1 cut(s) 625
TaqI TCGA 5 cut(s) 368, 603, 736, 894, 987
TaqII GACCGA 1 cut(s) 777
TasI AATT 1 cut(s) 47
TfiI GAWTC 3 cut(s) 77, 312, 532
Tru1I TTAA 3 cut(s) 170, 764, 1015
Tru9I TTAA 3 cut(s) 170, 764, 1015
TscAI CASTG 2 cut(s) 288, 433
TseFI GTSAC 5 cut(s) 138, 211, 578, 625, 685
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 5 cut(s) 138, 211, 578, 625, 685
TspDTI ATGAA 4 cut(s) 90, 146, 156, 940
TspGWI ACGGA 4 cut(s) 274, 500, 503, 785
TspRI CASTG 2 cut(s) 288, 433
XceI RCATGY 2 cut(s) 212, 854
XhoI CTCGAG 1 cut(s) 986
XmiI GTMKAC 1 cut(s) 603
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 2 cut(s) 54, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.