MD05G1255200.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001622
Physical Location & Seq
Reverse (-)
38566134 .. 38567695
1562 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1255200.v1.1.491

Sequence Viewer

Length: 789 bp
ATGTATTTGGGCCAAACTTACACTTATTCTATAGGTNATTCTGGAAGTCTCTTGGAGGGATTTACGAGGACTCAAGTTAGTACTCTTGCTGTAAAAGATAAGTTGCTAGTTGCTGGAGGATTCCAGGGTGAACTTATTTGCAAGCATCTTGATCGGCCTGGAGTCAGCTTTTGTTCAAGGACAACTAATGCAATAACAAATGCTGTTGAGATTTATGATAGTCCTAGCGGGGCAGTTCACTTTACAGCCTCTAATAATGACTGTGGAGTCAGAGACTTTGACGTGGAGAAATTTCAGCTTACTAAGCATTTCCGTTATCCTTGGCCTGTGAATCATACTTCTCTGAGTCCTGATGGTAAACTTCTTGTGATTGTTGGAGACAATCCGGAAGGGATGTTGGTAAACTCTCAAACTGGAAAGACGGTGATGTCTTTAAGTGGGCACTTGGATTTCTCCTTCGCATCAGCATGGCATCCTGATGGTATGACCTTTGCTACTGGGAACCAGGACAAAACCTGCCGGATTTGGGATGTTCGAAACCTTTCCAAGTCAGTTGCTGTTTTGAAGGGGAACCTTGGAGCTATTCGGTCAATCCGGTATACTTCCGATGGTCAGTATATGATGATGGCAGAGCCAGCGGATTTTGTGCATGTCTATGATGTGAAAAGTGGGTATGAAAAGGAACAGGAGATTGATTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCGGACACAGAGTCGCTTTTTATTGGAGTGTGGGATCGTACATACGGTAGTCTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

263

Amino Acids

28.78

Weight (kDa)

5.46

Isoelectric Point (pI)

22.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 32 - 224 1.3e-16 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 66 - 164 1.7e-07 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 72 - 182 2e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 74 - 256 1.3e-16 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 75 - 227 6.6e-13 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 84 - 208 1.1e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 110 - 256 1.7e-11 WDR3 first beta-propeller domain
WDR55 PF24796 112 - 224 3.3e-08 WDR55
WD40_CDC20-Fz PF24807 114 - 249 1.8e-08 CDC20/Fizzy WD40 domain
WD40 PF00400 139 - 177 5.3e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 156 - 255 5.7e-14 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 266, 427
Acc36I ACCTGC 1 cut(s) 524
AccI GTMKAC 1 cut(s) 599
AccIII TCCGGA 2 cut(s) 385, 730
AciI CCGC 2 cut(s) 228, 638
AclWI GGATC 1 cut(s) 771
AcsI RAATTY 1 cut(s) 290
AfaI GTAC 2 cut(s) 82, 769
AfiI CCNNNNNNNGG 1 cut(s) 526
AgsI TTSAA 2 cut(s) 177, 565
AhdI GACNNNNNGTC 1 cut(s) 739
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 3 cut(s) 123, 157, 504
AluBI AGCT 3 cut(s) 168, 298, 581
AluI AGCT 3 cut(s) 168, 298, 581
Alw26I GTCTC 3 cut(s) 53, 267, 372
AlwI GGATC 1 cut(s) 771
AlwNI CAGNNNCTG 1 cut(s) 557
Aor13HI TCCGGA 2 cut(s) 385, 730
AoxI GGCC 3 cut(s) 10, 155, 323
ApoI RAATTY 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 541
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 140, 436, 716
AsuII TTCGAA 1 cut(s) 535
BaeGI GKGCMC 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 773
BccI CCATC 4 cut(s) 347, 473, 602, 619
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 3 cut(s) 125, 159, 506
BcoDI GTCTC 3 cut(s) 53, 267, 372
BfaI CTAG 2 cut(s) 107, 225
BfmI CTRYAG 1 cut(s) 30
BfuAI ACCTGC 1 cut(s) 524
BmcAI AGTACT 1 cut(s) 82
Bme1390I CCNGG 3 cut(s) 125, 159, 506
BmeRI GACNNNNNGTC 1 cut(s) 739
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 2 cut(s) 503, 572
BmrFI CCNGG 3 cut(s) 125, 159, 506
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 3 cut(s) 154, 470, 481
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 1 cut(s) 773
BpmI CTGGAG 2 cut(s) 135, 180
Bpu14I TTCGAA 1 cut(s) 535
BpuEI CTTGAG 1 cut(s) 57
BsaJI CCNNGG 3 cut(s) 124, 320, 574
BsaWI WCCGGW 3 cut(s) 385, 594, 730
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse1I ACTGG 2 cut(s) 418, 502
BseAI TCCGGA 2 cut(s) 385, 730
BseBI CCWGG 3 cut(s) 125, 159, 506
BseDI CCNNGG 3 cut(s) 124, 320, 574
BseGI GGATG 3 cut(s) 399, 472, 535
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 2 cut(s) 418, 502
BseSI GKGCMC 1 cut(s) 444
BshFI GGCC 3 cut(s) 12, 157, 325
BsiSI CCGG 4 cut(s) 386, 520, 595, 731
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 3 cut(s) 53, 267, 372
BsnI GGCC 3 cut(s) 12, 157, 325
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 444
Bsp13I TCCGGA 2 cut(s) 385, 730
Bsp143I GATC 2 cut(s) 151, 763
BspACI CCGC 2 cut(s) 228, 638
BspANI GGCC 3 cut(s) 12, 157, 325
BspCNI CTCAG 1 cut(s) 336
BspEI TCCGGA 2 cut(s) 385, 730
BspLI GGNNCC 2 cut(s) 503, 572
BspMI ACCTGC 1 cut(s) 524
BspPI GGATC 1 cut(s) 771
BspT104I TTCGAA 1 cut(s) 535
BsrI ACTGG 2 cut(s) 418, 502
BssECI CCNNGG 3 cut(s) 124, 320, 574
BssMI GATC 2 cut(s) 151, 763
BssNAI GTATAC 1 cut(s) 600
BssT1I CCWWGG 2 cut(s) 320, 574
Bst1107I GTATAC 1 cut(s) 600
Bst2UI CCWGG 3 cut(s) 125, 159, 506
Bst4CI ACNGT 3 cut(s) 263, 424, 776
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 2 cut(s) 143, 636
BstDEI CTNAG 2 cut(s) 303, 344
BstF5I GGATG 3 cut(s) 399, 472, 535
BstKTI GATC 2 cut(s) 154, 766
BstMAI GTCTC 3 cut(s) 53, 267, 372
BstMBI GATC 2 cut(s) 151, 763
BstMWI GCNNNNNNNGC 2 cut(s) 304, 635
BstNI CCWGG 3 cut(s) 125, 159, 506
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 3 cut(s) 123, 157, 504
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 444
BstV2I GAAGAC 1 cut(s) 773
BstZ17I GTATAC 1 cut(s) 600
BsuRI GGCC 3 cut(s) 12, 157, 325
BtrI CACGTC 1 cut(s) 283
BtsCI GGATG 3 cut(s) 399, 472, 535
BveI ACCTGC 1 cut(s) 524
Cac8I GCNNGC 2 cut(s) 143, 636
CaiI CAGNNNCTG 1 cut(s) 557
Cfr13I GGNCC 1 cut(s) 10
Csp6I GTAC 2 cut(s) 81, 768
CviAII CATG 2 cut(s) 468, 650
CviJI RGCY 8 cut(s) 12, 157, 168, 248, 298, 325, 581, 634
CviKI_1 RGCY 8 cut(s) 12, 157, 168, 248, 298, 325, 581, 634
CviQI GTAC 2 cut(s) 81, 768
DdeI CTNAG 2 cut(s) 303, 344
DpnI GATC 2 cut(s) 153, 765
DpnII GATC 2 cut(s) 151, 763
DrdI GACNNNNNNGTC 2 cut(s) 266, 427
DriI GACNNNNNGTC 1 cut(s) 739
DseDI GACNNNNNNGTC 2 cut(s) 266, 427
Eam1105I GACNNNNNGTC 1 cut(s) 739
Eco130I CCWWGG 2 cut(s) 320, 574
Eco32I GATATC 1 cut(s) 711
EcoRII CCWGG 3 cut(s) 123, 157, 504
EcoRV GATATC 1 cut(s) 711
EcoT14I CCWWGG 2 cut(s) 320, 574
ErhI CCWWGG 2 cut(s) 320, 574
FaeI CATG 2 cut(s) 471, 653
FatI CATG 2 cut(s) 467, 649
FauI CCCGC 1 cut(s) 221
FblI GTMKAC 1 cut(s) 599
FokI GGATG 3 cut(s) 406, 459, 542
FspBI CTAG 2 cut(s) 107, 225
GsuI CTGGAG 2 cut(s) 135, 180
HaeIII GGCC 3 cut(s) 12, 157, 325
HapII CCGG 4 cut(s) 386, 520, 595, 731
Hin1II CATG 2 cut(s) 471, 653
HinfI GANTC 7 cut(s) 70, 120, 162, 267, 331, 346, 740
HpaII CCGG 4 cut(s) 386, 520, 595, 731
HphI GGTGA 3 cut(s) 140, 436, 716
Hpy166II GTNNAC 5 cut(s) 131, 238, 359, 403, 600
Hpy188I TCNGA 3 cut(s) 272, 345, 607
Hpy188III TCNNGA 6 cut(s) 42, 149, 350, 386, 476, 731
Hpy8I GTNNAC 5 cut(s) 131, 238, 359, 403, 600
HpyAV CCTTC 3 cut(s) 383, 466, 559
HpyCH4III ACNGT 3 cut(s) 263, 424, 776
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 3 cut(s) 141, 191, 649
HpyF10VI GCNNNNNNNGC 2 cut(s) 304, 635
HpyF3I CTNAG 2 cut(s) 303, 344
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 2 cut(s) 471, 653
Kpn2I TCCGGA 2 cut(s) 385, 730
Kzo9I GATC 2 cut(s) 151, 763
LmnI GCTCC 1 cut(s) 578
LweI GCATC 3 cut(s) 154, 470, 481
MaeI CTAG 2 cut(s) 107, 225
MaeII ACGT 1 cut(s) 282
MalI GATC 2 cut(s) 153, 765
MboI GATC 2 cut(s) 151, 763
MboII GAAGA 1 cut(s) 773
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 1 cut(s) 290
MlyI GAGTC 5 cut(s) 64, 171, 276, 355, 749
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 4 cut(s) 49, 60, 110, 259
MroI TCCGGA 2 cut(s) 385, 730
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 1 cut(s) 434
MslI CAYNNNNRTG 3 cut(s) 466, 477, 654
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 4 cut(s) 386, 520, 595, 731
MspR9I CCNGG 3 cut(s) 125, 159, 506
MvaI CCWGG 3 cut(s) 125, 159, 506
MwoI GCNNNNNNNGC 2 cut(s) 304, 635
NdeII GATC 2 cut(s) 151, 763
NlaIII CATG 2 cut(s) 471, 653
NlaIV GGNNCC 2 cut(s) 503, 572
NspI RCATGY 1 cut(s) 653
NspV TTCGAA 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 2 cut(s) 120, 331
PleI GAGTC 5 cut(s) 64, 170, 275, 354, 748
PpsI GAGTC 5 cut(s) 64, 170, 275, 354, 748
Psp6I CCWGG 3 cut(s) 123, 157, 504
PspGI CCWGG 3 cut(s) 123, 157, 504
PspN4I GGNNCC 2 cut(s) 503, 572
PspPI GGNCC 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 557
RsaI GTAC 2 cut(s) 82, 769
RsaNI GTAC 2 cut(s) 81, 768
RseI CAYNNNNRTG 3 cut(s) 466, 477, 654
SaqAI TTAA 1 cut(s) 434
Sau3AI GATC 2 cut(s) 151, 763
Sau96I GGNCC 1 cut(s) 10
ScaI AGTACT 1 cut(s) 82
SchI GAGTC 5 cut(s) 64, 171, 276, 355, 749
ScrFI CCNGG 3 cut(s) 125, 159, 506
SduI GDGCHC 1 cut(s) 444
SetI ASST 9 cut(s) 37, 170, 285, 300, 491, 518, 543, 576, 583
SfaNI GCATC 3 cut(s) 154, 470, 481
SfcI CTRYAG 1 cut(s) 30
SfuI TTCGAA 1 cut(s) 535
SmiMI CAYNNNNRTG 3 cut(s) 466, 477, 654
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 1 cut(s) 290
SsiI CCGC 2 cut(s) 228, 638
SspMI CTAG 2 cut(s) 107, 225
StyD4I CCNGG 3 cut(s) 123, 157, 504
StyI CCWWGG 2 cut(s) 320, 574
TaaI ACNGT 3 cut(s) 263, 424, 776
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 535
TaqII GACCGA 1 cut(s) 576
TasI AATT 1 cut(s) 290
TatI WGTACW 1 cut(s) 80
TfiI GAWTC 2 cut(s) 120, 331
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TspDTI ATGAA 1 cut(s) 690
TspGWI ACGGA 1 cut(s) 302
XapI RAATTY 1 cut(s) 290
XceI RCATGY 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 599
XmnI GAANNNNTTC 1 cut(s) 541
XspI CTAG 2 cut(s) 107, 225
ZrmI AGTACT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.