Rroxscaffold_1G00006870

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001622
Physical Location & Seq
Forward (+)
8863243 .. 8869054
5812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006870.1

Sequence Viewer

Length: 1332 bp
ATGTCCCACTACGCTGGTTACGTGGAAGATGAATATGAAATGGAAGATGTAGATGATGATATGGATGACGAATTTCGTGGAAGAGACCTGGCTGGCTCTGAGTCTGATGTTGATGAATTTGAATATGCGAATAGTAAAGTAGCTGATACAACTGCTGCTCAAGCAAGAAGAGGAAAAGACATCCAAGGTATTCCTTGGGATAGACTTAGCATTACTAGAGAGAAATACCGGCAAACTAGGTTAGAACAGTACAAGAATTATGAAAACATCCCTAATTCCGGAGAAGGGTCAGGGAAGCAGGATTGCAACATTACAAAGAAGGGATCTGTATATTATGAGTTTAGACGTAATGCAAGATCAGTGAAATCAACTATTCTTCATTTTCAGTTGCGGAACTTGGTCTGGGCTACATCAAAGCATGATGTTTACCTTATGTCACATTTTTCTGTCATTCATTGGTCTTCATTGAGTTGCAACAAGTCTGAGGTTCTAAATGTTTCAGGACATGTGGCACCATCAGAGAAACATCCCGGGAGTCTCTTGGAGGGATTTACGCAGACTCAAGTTAGTACTCTTGCAGTAAAAGATAAGTTGCTAGTTGCTGGAGGATTCCAGGGAGAACTTATTTGTAAGCATCTTGATCGGCCTGGAGTTAGCTTTTGTTCAAGGACAACTTACGATGATAATGCCATTACAAATGCTGTTGAGATTTATGATGGTCCCAGTGGTGCAGTTCACTTTACAGCATCAAATAATGATTGTGGAGTCAGAGACTTTGACATGGAGAAATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGTGAATCATACGTCTTTGAGTCCCAATGGCAAACTTCTTGTGATTGTTGGTGACAATCCAGATGGGATGTTGGTGGACTCTCAGACTGGAAAGACAGTGAAGTCTTTATCTGGGCACTTGGATTTCTCATTTGCATCAGCGTGGCATCCTGATGGTATCACCTTTGCTACTGGGAACCAGGACAAAACTTGTCGGGTTTGGGATGTTCGAAACCCATCTAAGTCGGTTGCTGTTTTAAAGGGAAACCTTGGAGCTATTCGGTCAATCCGTTATACTTCTGATGGTCGGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTATGAGAAGGAGCAGGAAATCGACTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCAGACACAGAGTCACTTTTCATCGGAGTGTGGGATCGTACTTATGGTAGTCTTCTCGAGTATGGACGACACCGGGATTACTCTTACCTAGATTCTCTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

443

Amino Acids

49.87

Weight (kDa)

5.41

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 195 - 390 9.1e-16 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 245 - 393 1.4e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 247 - 349 1.9e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 249 - 423 4.9e-15 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 249 - 346 1.3e-06 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 256 - 387 4e-07 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 278 - 415 2.3e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 278 - 422 8e-10 WDR3 first beta-propeller domain
WDR55 PF24796 278 - 390 2e-08 WDR55
WD40 PF00400 305 - 343 2e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 322 - 422 7.1e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 925
AccB1I GGYRCC 1 cut(s) 511
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 1 cut(s) 391
AclWI GGATC 2 cut(s) 331, 1269
AcoI YGGCCR 1 cut(s) 821
AcsI RAATTY 3 cut(s) 71, 116, 788
AfaI GTAC 3 cut(s) 251, 571, 1267
AfiI CCNNNNNNNGG 2 cut(s) 278, 285
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 2 cut(s) 122, 666
AhdI GACNNNNNGTC 1 cut(s) 1237
AjnI CCWGG 4 cut(s) 87, 612, 646, 1002
AluBI AGCT 4 cut(s) 143, 657, 796, 1079
AluI AGCT 4 cut(s) 143, 657, 796, 1079
Alw26I GTCTC 3 cut(s) 78, 542, 765
AlwI GGATC 2 cut(s) 331, 1269
Ama87I CYCGRG 2 cut(s) 530, 1283
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 2 cut(s) 644, 821
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 3 cut(s) 71, 116, 788
AspS9I GGNCC 1 cut(s) 719
AsuC2I CCSGG 3 cut(s) 531, 532, 1301
AsuHPI GGTGA 3 cut(s) 887, 976, 1214
AsuII TTCGAA 1 cut(s) 1033
AvaI CYCGRG 2 cut(s) 530, 1283
AvaII GGWCC 1 cut(s) 719
BaeGI GKGCMC 1 cut(s) 942
BalI TGGCCA 1 cut(s) 823
BanI GGYRCC 1 cut(s) 511
BbsI GAAGAC 2 cut(s) 453, 1271
BbvI GCAGC 1 cut(s) 142
BccI CCATC 7 cut(s) 523, 710, 881, 971, 1048, 1100, 1117
BcgI CGANNNNNNTGC 4 cut(s) 623, 657, 668, 702
BciT130I CCWGG 4 cut(s) 89, 614, 648, 1004
BcnI CCSGG 3 cut(s) 531, 532, 1301
BcoDI GTCTC 3 cut(s) 78, 542, 765
BfaI CTAG 4 cut(s) 216, 237, 596, 1316
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
BmcAI AGTACT 1 cut(s) 571
Bme1390I CCNGG 7 cut(s) 89, 531, 532, 614, 648, 1004, 1301
Bme18I GGWCC 1 cut(s) 719
BmeRI GACNNNNNGTC 1 cut(s) 1237
BmeT110I CYCGRG 2 cut(s) 530, 1283
BmgT120I GGNCC 1 cut(s) 719
BmiI GGNNCC 3 cut(s) 513, 721, 1001
BmrFI CCNGG 7 cut(s) 89, 531, 532, 614, 648, 1004, 1301
BmrI ACTGGG 2 cut(s) 717, 1005
BmsI GCATC 4 cut(s) 643, 755, 968, 979
BmuI ACTGGG 2 cut(s) 717, 1005
BpiI GAAGAC 2 cut(s) 453, 1271
BpmI CTGGAG 2 cut(s) 624, 669
Bpu14I TTCGAA 1 cut(s) 1033
BpuEI CTTGAG 2 cut(s) 144, 546
BpuMI CCSGG 3 cut(s) 531, 532, 1301
BsaAI YACGTR 1 cut(s) 22
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 6 cut(s) 184, 194, 530, 613, 818, 1072
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 285
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 4 cut(s) 723, 824, 916, 1000
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 4 cut(s) 89, 614, 648, 1004
BseDI CCNNGG 6 cut(s) 184, 194, 530, 613, 818, 1072
BseGI GGATG 7 cut(s) 70, 180, 267, 526, 897, 970, 1033
BseLI CCNNNNNNNGG 2 cut(s) 278, 285
BseMII CTCAG 3 cut(s) 90, 474, 920
BseNI ACTGG 4 cut(s) 723, 824, 916, 1000
BseSI GKGCMC 1 cut(s) 942
BseXI GCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 750
BshFI GGCC 2 cut(s) 646, 823
BshNI GGYRCC 1 cut(s) 511
BsiHKCI CYCGRG 2 cut(s) 530, 1283
BsiSI CCGG 4 cut(s) 229, 279, 531, 1300
BslFI GGGAC 2 cut(s) 705, 831
BslI CCNNNNNNNGG 2 cut(s) 278, 285
BsmAI GTCTC 3 cut(s) 78, 542, 765
BsmFI GGGAC 2 cut(s) 705, 831
BsnI GGCC 2 cut(s) 646, 823
Bso31I GGTCTC 1 cut(s) 78
BsoBI CYCGRG 2 cut(s) 530, 1283
Bsp119I TTCGAA 1 cut(s) 1033
Bsp1286I GDGCHC 1 cut(s) 942
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 4 cut(s) 323, 356, 640, 1261
BspACI CCGC 1 cut(s) 391
BspANI GGCC 2 cut(s) 646, 823
BspCNI CTCAG 3 cut(s) 91, 475, 919
BspEI TCCGGA 1 cut(s) 278
BspLI GGNNCC 3 cut(s) 513, 721, 1001
BspPI GGATC 2 cut(s) 331, 1269
BspT104I TTCGAA 1 cut(s) 1033
BspT107I GGYRCC 1 cut(s) 511
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 4 cut(s) 723, 824, 916, 1000
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 6 cut(s) 184, 194, 530, 613, 818, 1072
BssMI GATC 4 cut(s) 323, 356, 640, 1261
BssT1I CCWWGG 4 cut(s) 184, 194, 818, 1072
Bst2UI CCWGG 4 cut(s) 89, 614, 648, 1004
Bst4CI ACNGT 2 cut(s) 249, 922
Bst6I CTCTTC 2 cut(s) 76, 163
BstBAI YACGTR 1 cut(s) 22
BstBI TTCGAA 1 cut(s) 1033
BstC8I GCNNGC 2 cut(s) 94, 1134
BstDEI CTNAG 6 cut(s) 99, 206, 483, 801, 906, 1044
BstF5I GGATG 7 cut(s) 70, 180, 267, 526, 897, 970, 1033
BstKTI GATC 4 cut(s) 326, 359, 643, 1264
BstMAI GTCTC 3 cut(s) 78, 542, 765
BstMBI GATC 4 cut(s) 323, 356, 640, 1261
BstMWI GCNNNNNNNGC 3 cut(s) 161, 802, 1133
BstNI CCWGG 4 cut(s) 89, 614, 648, 1004
BstNSI RCATGY 2 cut(s) 509, 1151
BstSCI CCNGG 7 cut(s) 87, 529, 530, 612, 646, 1002, 1299
BstSLI GKGCMC 1 cut(s) 942
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 2 cut(s) 453, 1271
BstX2I RGATCY 1 cut(s) 323
BstXI CCANNNNNNTGG 1 cut(s) 14
BstYI RGATCY 1 cut(s) 323
BsuRI GGCC 2 cut(s) 646, 823
BtsCI GGATG 7 cut(s) 70, 180, 267, 526, 897, 970, 1033
BtsIMutI CAGTG 4 cut(s) 366, 730, 831, 927
Cac8I GCNNGC 2 cut(s) 94, 1134
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 719
Cfr9I CCCGGG 1 cut(s) 530
Csp6I GTAC 3 cut(s) 250, 570, 1266
CviAII CATG 4 cut(s) 419, 506, 781, 1148
CviQI GTAC 3 cut(s) 250, 570, 1266
DdeI CTNAG 6 cut(s) 99, 206, 483, 801, 906, 1044
DpnI GATC 4 cut(s) 325, 358, 642, 1263
DpnII GATC 4 cut(s) 323, 356, 640, 1261
DraI TTTAAA 1 cut(s) 1062
DrdI GACNNNNNNGTC 1 cut(s) 925
DriI GACNNNNNGTC 1 cut(s) 1237
DseDI GACNNNNNNGTC 1 cut(s) 925
EaeI YGGCCR 1 cut(s) 821
Eam1104I CTCTTC 2 cut(s) 76, 163
Eam1105I GACNNNNNGTC 1 cut(s) 1237
EarI CTCTTC 2 cut(s) 76, 163
Eco130I CCWWGG 4 cut(s) 184, 194, 818, 1072
Eco31I GGTCTC 1 cut(s) 78
Eco32I GATATC 1 cut(s) 1209
Eco47I GGWCC 1 cut(s) 719
Eco88I CYCGRG 2 cut(s) 530, 1283
EcoRII CCWGG 4 cut(s) 87, 612, 646, 1002
EcoRV GATATC 1 cut(s) 1209
EcoT14I CCWWGG 4 cut(s) 184, 194, 818, 1072
ErhI CCWWGG 4 cut(s) 184, 194, 818, 1072
FaeI CATG 4 cut(s) 422, 509, 784, 1151
FalI AAGNNNNNCTT 2 cut(s) 658, 690
FaqI GGGAC 2 cut(s) 705, 831
FatI CATG 4 cut(s) 418, 505, 780, 1147
Fnu4HI GCNGC 1 cut(s) 156
FokI GGATG 7 cut(s) 77, 167, 254, 513, 904, 957, 1040
Fsp4HI GCNGC 1 cut(s) 156
FspBI CTAG 4 cut(s) 216, 237, 596, 1316
GluI GCNGC 1 cut(s) 156
GsuI CTGGAG 2 cut(s) 624, 669
HaeIII GGCC 2 cut(s) 646, 823
HapII CCGG 4 cut(s) 229, 279, 531, 1300
Hin1II CATG 4 cut(s) 422, 509, 784, 1151
HpaII CCGG 4 cut(s) 229, 279, 531, 1300
HphI GGTGA 3 cut(s) 887, 976, 1214
Hpy166II GTNNAC 3 cut(s) 427, 736, 901
Hpy188I TCNGA 8 cut(s) 100, 106, 484, 520, 770, 909, 1105, 1253
Hpy188III TCNNGA 7 cut(s) 279, 501, 638, 884, 974, 1229, 1283
Hpy8I GTNNAC 3 cut(s) 427, 736, 901
HpyAV CCTTC 3 cut(s) 278, 313, 1171
HpyCH4III ACNGT 2 cut(s) 249, 922
HpyCH4IV ACGT 3 cut(s) 21, 346, 836
HpyCH4V TGCA 7 cut(s) 306, 353, 474, 578, 731, 959, 1147
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 802, 1133
HpyF3I CTNAG 6 cut(s) 99, 206, 483, 801, 906, 1044
HpySE526I ACGT 3 cut(s) 21, 346, 836
Hsp92II CATG 4 cut(s) 422, 509, 784, 1151
Kpn2I TCCGGA 1 cut(s) 278
Kzo9I GATC 4 cut(s) 323, 356, 640, 1261
LmnI GCTCC 2 cut(s) 1076, 1180
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 4 cut(s) 643, 755, 968, 979
MaeI CTAG 4 cut(s) 216, 237, 596, 1316
MaeII ACGT 3 cut(s) 21, 346, 836
MaeIII GTNAC 4 cut(s) 17, 435, 875, 1239
MalI GATC 4 cut(s) 325, 358, 642, 1263
MboI GATC 4 cut(s) 323, 356, 640, 1261
MboII GAAGA 7 cut(s) 38, 56, 93, 180, 368, 453, 1271
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 1 cut(s) 942
MlsI TGGCCA 1 cut(s) 823
MluCI AATT 5 cut(s) 71, 116, 256, 274, 788
MluNI TGGCCA 1 cut(s) 823
MlyI GAGTC 7 cut(s) 110, 544, 553, 774, 853, 896, 1247
MnlI CCTC 4 cut(s) 164, 478, 538, 599
Mox20I TGGCCA 1 cut(s) 823
MroI TCCGGA 1 cut(s) 278
MscI TGGCCA 1 cut(s) 823
MseI TTAA 2 cut(s) 1061, 1330
MslI CAYNNNNRTG 4 cut(s) 964, 975, 1152, 1253
Msp20I TGGCCA 1 cut(s) 823
MspI CCGG 4 cut(s) 229, 279, 531, 1300
MspR9I CCNGG 7 cut(s) 89, 531, 532, 614, 648, 1004, 1301
MvaI CCWGG 4 cut(s) 89, 614, 648, 1004
MwoI GCNNNNNNNGC 3 cut(s) 161, 802, 1133
NciI CCSGG 3 cut(s) 531, 532, 1301
NdeII GATC 4 cut(s) 323, 356, 640, 1261
NlaIII CATG 4 cut(s) 422, 509, 784, 1151
NlaIV GGNNCC 3 cut(s) 513, 721, 1001
NmuCI GTSAC 3 cut(s) 435, 875, 1239
NspI RCATGY 2 cut(s) 509, 1151
NspV TTCGAA 1 cut(s) 1033
PaeR7I CTCGAG 1 cut(s) 1283
PciI ACATGT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 1090
PfeI GAWTC 3 cut(s) 609, 829, 1319
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 7 cut(s) 109, 543, 553, 773, 852, 896, 1246
PpsI GAGTC 7 cut(s) 109, 543, 553, 773, 852, 896, 1246
Ppu21I YACGTR 1 cut(s) 22
PscI ACATGT 1 cut(s) 505
Psp6I CCWGG 4 cut(s) 87, 612, 646, 1002
PspGI CCWGG 4 cut(s) 87, 612, 646, 1002
PspN4I GGNNCC 3 cut(s) 513, 721, 1001
PspPI GGNCC 1 cut(s) 719
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 3 cut(s) 251, 571, 1267
RsaNI GTAC 3 cut(s) 250, 570, 1266
RseI CAYNNNNRTG 4 cut(s) 964, 975, 1152, 1253
SaqAI TTAA 2 cut(s) 1061, 1330
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 4 cut(s) 323, 356, 640, 1261
Sau96I GGNCC 1 cut(s) 719
ScaI AGTACT 1 cut(s) 571
SchI GAGTC 7 cut(s) 110, 544, 553, 774, 853, 896, 1247
ScrFI CCNGG 7 cut(s) 89, 531, 532, 614, 648, 1004, 1301
SduI GDGCHC 1 cut(s) 942
SfaNI GCATC 4 cut(s) 643, 755, 968, 979
Sfr274I CTCGAG 1 cut(s) 1283
SfuI TTCGAA 1 cut(s) 1033
SinI GGWCC 1 cut(s) 719
SlaI CTCGAG 1 cut(s) 1283
SmaI CCCGGG 1 cut(s) 532
SmiMI CAYNNNNRTG 4 cut(s) 964, 975, 1152, 1253
SmlI CTYRAG 3 cut(s) 159, 561, 1283
SmoI CTYRAG 3 cut(s) 159, 561, 1283
Sse9I AATT 5 cut(s) 71, 116, 256, 274, 788
SsiI CCGC 1 cut(s) 391
SspMI CTAG 4 cut(s) 216, 237, 596, 1316
StyD4I CCNGG 7 cut(s) 87, 529, 530, 612, 646, 1002, 1299
StyI CCWWGG 4 cut(s) 184, 194, 818, 1072
TaaI ACNGT 2 cut(s) 249, 922
TaiI ACGT 3 cut(s) 24, 349, 839
TaqI TCGA 3 cut(s) 1033, 1191, 1284
TaqII GACCGA 1 cut(s) 1074
TasI AATT 5 cut(s) 71, 116, 256, 274, 788
TatI WGTACW 2 cut(s) 249, 569
TfiI GAWTC 3 cut(s) 609, 829, 1319
Tru1I TTAA 2 cut(s) 1061, 1330
Tru9I TTAA 2 cut(s) 1061, 1330
TscAI CASTG 4 cut(s) 366, 730, 831, 927
TseFI GTSAC 3 cut(s) 435, 875, 1239
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 3 cut(s) 435, 875, 1239
TspDTI ATGAA 8 cut(s) 45, 51, 129, 276, 368, 443, 453, 1237
TspGWI ACGGA 2 cut(s) 800, 1082
TspMI CCCGGG 1 cut(s) 530
TspRI CASTG 4 cut(s) 366, 730, 831, 927
VpaK11BI GGWCC 1 cut(s) 719
XapI RAATTY 3 cut(s) 71, 116, 788
XceI RCATGY 2 cut(s) 509, 1151
XhoI CTCGAG 1 cut(s) 1283
XmaI CCCGGG 1 cut(s) 530
XspI CTAG 4 cut(s) 216, 237, 596, 1316
ZrmI AGTACT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.