Rh5BG511800

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001622
Physical Location & Seq
Reverse (-)
81318330 .. 81321075
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG511800.1

Sequence Viewer

Length: 1131 bp
ATGTCCCACTACGCTGGTTACGTGGAAGATGAATATGAAATGGAAGATGTAGATGATGATATGGATGACGAATTTCGTGGAAGAGACCTGGCTGGCTCAGAGTCTGATGTTGATGAATTTGAATATGCGAATAGTAAAGTAGCTGATACAACTGCTGCTCAAGCAAGAAGAGGAAAAGACATCCAAGGTATTCCTTGGGATAGACTTAGCATTACTAGAGAAAAATACCGGCAAACTAGGTTAGAACAGTACAAGAATTATGAAAACATCCCTAATTCCGGAGAAGGGTCAGGGAAGGATTGCAACATTACAAAGAAGGGATCTGTATATTATGAGTTTAGACGTAATGCAAGATCAGTGAAATCAACTATTCTTCATTTTCAGTTGCGGAACTTGGTCTGGGCTACATCAAAGCATGATGTCTACCTTATGTCACATTTTTCTGTCATTCATTGGTCTTCATTGAGTTGCAACAAGTCTGAGGTTCTAAATGTTTCAGGACATGTGGCACCATCAGAGAAACATCCCGGGAGTCTCTTGGAGGGATTTACGCAGACTCAAGTTAGTACTCTTGCAGTAAAAGATAAGTTGCTGGTTGCTGGAGGATTCCAGGGAGAACTTATTTGTAAGCATACTTCTTTGAGTCCCAGTGGCAAACTTCTTGTGATTGTTGGTGACAATCCAGATGGGATGTTGGTGGACTCTCAGACTGGAAAGACAGTGGCGGCTTTATCTGGGCACTTGGATTTCTCATTTGCATCAGCGTGGCATCCTGATGGTATCACCTTTGCTACTGGGAACCAGGACAAAACTTGTCGGGTTTGGGATGTTCGAAACCTATCCAAGTCGGTTGCTGTTTTAAAGGGAAACCTTGGAGCTATTCGGTCAATCCGTTATACTTCTGATGGTCGGTATATGATGATGGCAGAGCCAGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTATGAGAAGGAGCAGGAAATCGACTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCAGACACAGAGTCACTTTTCATTGGAGTGTGGGATCGTACGTATGGTAGTCTTCTCGAGTATGGACGACACCGGGATTACTCTTACCTAGATTCTCTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

376

Amino Acids

42.18

Weight (kDa)

5.34

Isoelectric Point (pI)

28.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 202 - 319 1.5e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 209 - 325 1.4e-08 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 209 - 323 1.2e-08 WDR55
WD40_Prp19 PF24814 211 - 323 4.7e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 212 - 356 6.8e-13 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 213 - 348 5.4e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 215 - 354 3e-15 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 232 - 355 7.9e-10 WDR3 first beta-propeller domain
WD40 PF00400 238 - 276 5.4e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 255 - 354 6.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 423
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 2 cut(s) 388, 725
AclWI GGATC 2 cut(s) 328, 1068
AcsI RAATTY 2 cut(s) 71, 116
AfaI GTAC 3 cut(s) 251, 568, 1066
AfiI CCNNNNNNNGG 2 cut(s) 278, 285
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 1 cut(s) 122
AhdI GACNNNNNGTC 1 cut(s) 1036
AjnI CCWGG 3 cut(s) 87, 609, 801
AluBI AGCT 2 cut(s) 143, 878
AluI AGCT 2 cut(s) 143, 878
Alw26I GTCTC 2 cut(s) 78, 539
AlwI GGATC 2 cut(s) 328, 1068
AlwNI CAGNNNCTG 1 cut(s) 104
Ama87I CYCGRG 2 cut(s) 527, 1082
Aor13HI TCCGGA 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 2 cut(s) 71, 116
AsuC2I CCSGG 3 cut(s) 528, 529, 1100
AsuHPI GGTGA 3 cut(s) 686, 775, 1013
AsuII TTCGAA 1 cut(s) 832
AvaI CYCGRG 2 cut(s) 527, 1082
BaeGI GKGCMC 1 cut(s) 741
BanI GGYRCC 1 cut(s) 508
BarI GAAGNNNNNNTAC 2 cut(s) 619, 651
BbsI GAAGAC 2 cut(s) 450, 1070
BbvI GCAGC 1 cut(s) 142
BccI CCATC 5 cut(s) 520, 680, 770, 899, 916
BciT130I CCWGG 3 cut(s) 89, 611, 803
BcnI CCSGG 3 cut(s) 528, 529, 1100
BcoDI GTCTC 2 cut(s) 78, 539
BfaI CTAG 3 cut(s) 216, 237, 1115
BisI GCNGC 2 cut(s) 156, 726
BlsI GCNGC 2 cut(s) 157, 727
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 6 cut(s) 89, 528, 529, 611, 803, 1100
BmeRI GACNNNNNGTC 1 cut(s) 1036
BmeT110I CYCGRG 2 cut(s) 527, 1082
BmiI GGNNCC 2 cut(s) 510, 800
BmrFI CCNGG 6 cut(s) 89, 528, 529, 611, 803, 1100
BmrI ACTGGG 2 cut(s) 642, 804
BmsI GCATC 2 cut(s) 767, 778
BmuI ACTGGG 2 cut(s) 642, 804
BpiI GAAGAC 2 cut(s) 450, 1070
BpmI CTGGAG 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 832
BpuEI CTTGAG 2 cut(s) 144, 543
BpuMI CCSGG 3 cut(s) 528, 529, 1100
BsaAI YACGTR 2 cut(s) 22, 1068
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 5 cut(s) 184, 194, 527, 610, 871
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 285
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 3 cut(s) 648, 715, 799
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 3 cut(s) 89, 611, 803
BseDI CCNNGG 5 cut(s) 184, 194, 527, 610, 871
BseGI GGATG 7 cut(s) 70, 180, 267, 523, 696, 769, 832
BseLI CCNNNNNNNGG 2 cut(s) 278, 285
BseMII CTCAG 3 cut(s) 111, 471, 719
BseNI ACTGG 3 cut(s) 648, 715, 799
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 508
BsiHKCI CYCGRG 2 cut(s) 527, 1082
BsiSI CCGG 4 cut(s) 229, 279, 528, 1099
BsiWI CGTACG 1 cut(s) 1064
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 2 cut(s) 278, 285
BsmAI GTCTC 2 cut(s) 78, 539
BsmFI GGGAC 1 cut(s) 630
Bso31I GGTCTC 1 cut(s) 78
BsoBI CYCGRG 2 cut(s) 527, 1082
Bsp119I TTCGAA 1 cut(s) 832
Bsp1286I GDGCHC 1 cut(s) 741
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 3 cut(s) 320, 353, 1060
BspACI CCGC 2 cut(s) 388, 725
BspCNI CTCAG 3 cut(s) 110, 472, 718
BspEI TCCGGA 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 510, 800
BspPI GGATC 2 cut(s) 328, 1068
BspT104I TTCGAA 1 cut(s) 832
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 3 cut(s) 648, 715, 799
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 5 cut(s) 184, 194, 527, 610, 871
BssMI GATC 3 cut(s) 320, 353, 1060
BssT1I CCWWGG 3 cut(s) 184, 194, 871
Bst2UI CCWGG 3 cut(s) 89, 611, 803
Bst4CI ACNGT 2 cut(s) 249, 721
Bst6I CTCTTC 2 cut(s) 76, 163
BstBAI YACGTR 2 cut(s) 22, 1068
BstBI TTCGAA 1 cut(s) 832
BstC8I GCNNGC 2 cut(s) 94, 933
BstDEI CTNAG 4 cut(s) 97, 206, 480, 705
BstF5I GGATG 7 cut(s) 70, 180, 267, 523, 696, 769, 832
BstKTI GATC 3 cut(s) 323, 356, 1063
BstMAI GTCTC 2 cut(s) 78, 539
BstMBI GATC 3 cut(s) 320, 353, 1060
BstMWI GCNNNNNNNGC 2 cut(s) 161, 932
BstNI CCWGG 3 cut(s) 89, 611, 803
BstNSI RCATGY 2 cut(s) 506, 950
BstSCI CCNGG 6 cut(s) 87, 526, 527, 609, 801, 1098
BstSLI GKGCMC 1 cut(s) 741
BstSNI TACGTA 1 cut(s) 1068
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 2 cut(s) 450, 1070
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 14
BstYI RGATCY 1 cut(s) 320
BtsCI GGATG 7 cut(s) 70, 180, 267, 523, 696, 769, 832
BtsIMutI CAGTG 3 cut(s) 363, 655, 726
Cac8I GCNNGC 2 cut(s) 94, 933
CaiI CAGNNNCTG 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 228
Cfr9I CCCGGG 1 cut(s) 527
Csp6I GTAC 3 cut(s) 250, 567, 1065
CviAII CATG 3 cut(s) 416, 503, 947
CviJI RGCY 7 cut(s) 92, 96, 143, 404, 728, 878, 931
CviKI_1 RGCY 7 cut(s) 92, 96, 143, 404, 728, 878, 931
CviQI GTAC 3 cut(s) 250, 567, 1065
DdeI CTNAG 4 cut(s) 97, 206, 480, 705
DpnI GATC 3 cut(s) 322, 355, 1062
DpnII GATC 3 cut(s) 320, 353, 1060
DraI TTTAAA 1 cut(s) 861
DriI GACNNNNNGTC 1 cut(s) 1036
Eam1104I CTCTTC 2 cut(s) 76, 163
Eam1105I GACNNNNNGTC 1 cut(s) 1036
EarI CTCTTC 2 cut(s) 76, 163
Eco105I TACGTA 1 cut(s) 1068
Eco130I CCWWGG 3 cut(s) 184, 194, 871
Eco31I GGTCTC 1 cut(s) 78
Eco32I GATATC 1 cut(s) 1008
Eco88I CYCGRG 2 cut(s) 527, 1082
EcoRII CCWGG 3 cut(s) 87, 609, 801
EcoRV GATATC 1 cut(s) 1008
EcoT14I CCWWGG 3 cut(s) 184, 194, 871
ErhI CCWWGG 3 cut(s) 184, 194, 871
FaeI CATG 3 cut(s) 419, 506, 950
FaqI GGGAC 1 cut(s) 630
FatI CATG 3 cut(s) 415, 502, 946
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 156, 726
FokI GGATG 7 cut(s) 77, 167, 254, 510, 703, 756, 839
Fsp4HI GCNGC 2 cut(s) 156, 726
FspBI CTAG 3 cut(s) 216, 237, 1115
GluI GCNGC 2 cut(s) 156, 726
GsuI CTGGAG 1 cut(s) 621
HapII CCGG 4 cut(s) 229, 279, 528, 1099
Hin1II CATG 3 cut(s) 419, 506, 950
HinfI GANTC 8 cut(s) 101, 532, 556, 606, 643, 701, 1037, 1118
HpaII CCGG 4 cut(s) 229, 279, 528, 1099
HphI GGTGA 3 cut(s) 686, 775, 1013
Hpy166II GTNNAC 2 cut(s) 424, 700
Hpy188I TCNGA 6 cut(s) 100, 106, 481, 517, 708, 904
Hpy188III TCNNGA 6 cut(s) 279, 498, 683, 773, 1028, 1082
Hpy8I GTNNAC 2 cut(s) 424, 700
HpyAV CCTTC 4 cut(s) 278, 289, 310, 970
HpyCH4III ACNGT 2 cut(s) 249, 721
HpyCH4IV ACGT 3 cut(s) 21, 343, 1067
HpyCH4V TGCA 6 cut(s) 303, 350, 471, 575, 758, 946
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 932
HpyF3I CTNAG 4 cut(s) 97, 206, 480, 705
HpySE526I ACGT 3 cut(s) 21, 343, 1067
Hsp92II CATG 3 cut(s) 419, 506, 950
Kpn2I TCCGGA 1 cut(s) 278
Kzo9I GATC 3 cut(s) 320, 353, 1060
LmnI GCTCC 2 cut(s) 875, 979
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 2 cut(s) 767, 778
MaeI CTAG 3 cut(s) 216, 237, 1115
MaeII ACGT 3 cut(s) 21, 343, 1067
MaeIII GTNAC 4 cut(s) 17, 432, 674, 1038
MalI GATC 3 cut(s) 322, 355, 1062
MboI GATC 3 cut(s) 320, 353, 1060
MboII GAAGA 7 cut(s) 38, 56, 93, 180, 365, 450, 1070
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 1 cut(s) 741
MluCI AATT 4 cut(s) 71, 116, 256, 274
MlyI GAGTC 6 cut(s) 110, 541, 550, 652, 695, 1046
MnlI CCTC 4 cut(s) 164, 475, 535, 596
MroI TCCGGA 1 cut(s) 278
MseI TTAA 2 cut(s) 860, 1129
MslI CAYNNNNRTG 4 cut(s) 763, 774, 951, 1052
MspI CCGG 4 cut(s) 229, 279, 528, 1099
MspR9I CCNGG 6 cut(s) 89, 528, 529, 611, 803, 1100
MvaI CCWGG 3 cut(s) 89, 611, 803
MwoI GCNNNNNNNGC 2 cut(s) 161, 932
NciI CCSGG 3 cut(s) 528, 529, 1100
NdeII GATC 3 cut(s) 320, 353, 1060
NlaIII CATG 3 cut(s) 419, 506, 950
NlaIV GGNNCC 2 cut(s) 510, 800
NmuCI GTSAC 3 cut(s) 432, 674, 1038
NspI RCATGY 2 cut(s) 506, 950
NspV TTCGAA 1 cut(s) 832
PaeR7I CTCGAG 1 cut(s) 1082
PciI ACATGT 1 cut(s) 502
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 889
PfeI GAWTC 2 cut(s) 606, 1118
Pfl23II CGTACG 1 cut(s) 1064
PkrI GCNGC 2 cut(s) 157, 727
PleI GAGTC 6 cut(s) 109, 540, 550, 651, 695, 1045
PpsI GAGTC 6 cut(s) 109, 540, 550, 651, 695, 1045
Ppu21I YACGTR 2 cut(s) 22, 1068
PscI ACATGT 1 cut(s) 502
Psp6I CCWGG 3 cut(s) 87, 609, 801
PspGI CCWGG 3 cut(s) 87, 609, 801
PspLI CGTACG 1 cut(s) 1064
PspN4I GGNNCC 2 cut(s) 510, 800
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 320
RsaI GTAC 3 cut(s) 251, 568, 1066
RsaNI GTAC 3 cut(s) 250, 567, 1065
RseI CAYNNNNRTG 4 cut(s) 763, 774, 951, 1052
SaqAI TTAA 2 cut(s) 860, 1129
SatI GCNGC 2 cut(s) 156, 726
Sau3AI GATC 3 cut(s) 320, 353, 1060
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 6 cut(s) 110, 541, 550, 652, 695, 1046
ScrFI CCNGG 6 cut(s) 89, 528, 529, 611, 803, 1100
SduI GDGCHC 1 cut(s) 741
SfaNI GCATC 2 cut(s) 767, 778
Sfr274I CTCGAG 1 cut(s) 1082
SfuI TTCGAA 1 cut(s) 832
SlaI CTCGAG 1 cut(s) 1082
SmaI CCCGGG 1 cut(s) 529
SmiMI CAYNNNNRTG 4 cut(s) 763, 774, 951, 1052
SmlI CTYRAG 3 cut(s) 159, 558, 1082
SmoI CTYRAG 3 cut(s) 159, 558, 1082
SnaBI TACGTA 1 cut(s) 1068
Sse9I AATT 4 cut(s) 71, 116, 256, 274
SsiI CCGC 2 cut(s) 388, 725
SspMI CTAG 3 cut(s) 216, 237, 1115
StyD4I CCNGG 6 cut(s) 87, 526, 527, 609, 801, 1098
StyI CCWWGG 3 cut(s) 184, 194, 871
TaaI ACNGT 2 cut(s) 249, 721
TaiI ACGT 3 cut(s) 24, 346, 1070
TaqI TCGA 3 cut(s) 832, 990, 1083
TaqII GACCGA 1 cut(s) 873
TasI AATT 4 cut(s) 71, 116, 256, 274
TatI WGTACW 2 cut(s) 249, 566
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 2 cut(s) 606, 1118
Tru1I TTAA 2 cut(s) 860, 1129
Tru9I TTAA 2 cut(s) 860, 1129
TscAI CASTG 3 cut(s) 363, 655, 726
TseFI GTSAC 3 cut(s) 432, 674, 1038
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 3 cut(s) 432, 674, 1038
TspDTI ATGAA 8 cut(s) 45, 51, 129, 276, 365, 440, 450, 1036
TspGWI ACGGA 1 cut(s) 881
TspMI CCCGGG 1 cut(s) 527
TspRI CASTG 3 cut(s) 363, 655, 726
XapI RAATTY 2 cut(s) 71, 116
XceI RCATGY 2 cut(s) 506, 950
XhoI CTCGAG 1 cut(s) 1082
XmaI CCCGGG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 3 cut(s) 216, 237, 1115
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.