MD03G1216600.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001622
Physical Location & Seq
Reverse (-)
29747326 .. 29751034
3709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1216600.v1.1.491

Sequence Viewer

Length: 954 bp
ATGTTGATCTATAGTGTTGATTTTTGTGGCATTGTTGGGAAGAATATGTCCCATTACGCTGGTTATGTGGAGGATGAATACGAAATGGAAGATGTAGACGATGATATGGATGATGAATTTCGCGGTAGAGAAATGGGCGGCTCAGATTCTGATGTTGATGAATATGACTACTCGCGACATACTAGGTTTGAACAATACAAGAATTATGAGAGTATTCCTAATTCTGGAGAAGGATCAGGGAAGGATTGCAAAGATACAAAGAAAGGATCTGTATATTATGAGTTCAGACGAAGTACTAGATCAGTGAAATCAACCATTCTTCATTTTCAGTTGCGTAACCTGGTCTGGGCTACTTCCAAGCATGATGTCTACCTGATGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCACAAAGTCTGAGGTTCTCAATGTTTCTGGACATGTGGCACCATCGGAGAAACATTCTGGAAGTCTCTTGGAGGGATTTACGAGGACTCAAGTTAGTACTCTTGCTGTAAAAGATAAGTTGCTAGTTGCTGGAGGATTCCAGGGTGAACTTATTTGCAAGCATCTTGATCGGCCTGGAGTCAGCTTTTGTTCAAGGACAACTAATGCAATAACAAATGCTGTTGAGATTTATGATAGTCCTAGCGGGGCAGTTCACTTTACAGCCTCTAATAATGACTGTGGAGTCAGAGACTTTGACGTGGAGAAATTTCAGCTTACTAAGCATTTCCGTTATCCTTGGCCTGTGAATCATACTTCTCTGAGTCCTGATGGTAAACTTCTTGTGATTGTTGGAGACAATCCGGAAGGGATGTTGGTAAACTCTCAAACTGGAAAGGTATGTATCTCAGACTTTGACAGACTTCCTGAGCTAGGAAAGGACGGTGTGCTGATTTGTATGAGTTTCTTTGCAGACGTGATAGTGCATTTTGAAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

318

Amino Acids

35.59

Weight (kDa)

5.27

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 454
AccI GTMKAC 2 cut(s) 96, 369
AccII CGCG 2 cut(s) 123, 175
AccIII TCCGGA 1 cut(s) 817
AciI CCGC 3 cut(s) 123, 138, 660
AclWI GGATC 2 cut(s) 241, 274
AcsI RAATTY 2 cut(s) 116, 722
AfaI GTAC 2 cut(s) 295, 514
AfiI CCNNNNNNNGG 3 cut(s) 224, 346, 887
AflIII ACRYGT 1 cut(s) 448
AgsI TTSAA 3 cut(s) 191, 609, 947
AjiI CACGTC 2 cut(s) 715, 931
AjnI CCWGG 3 cut(s) 339, 555, 589
AluBI AGCT 3 cut(s) 600, 730, 886
AluI AGCT 3 cut(s) 600, 730, 886
Alw26I GTCTC 3 cut(s) 485, 699, 804
AlwI GGATC 2 cut(s) 241, 274
AlwNI CAGNNNCTG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 817
AoxI GGCC 2 cut(s) 587, 755
ApoI RAATTY 2 cut(s) 116, 722
AsuHPI GGTGA 1 cut(s) 572
BanI GGYRCC 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 396
BccI CCATC 2 cut(s) 466, 779
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 3 cut(s) 341, 557, 591
BcoDI GTCTC 3 cut(s) 485, 699, 804
BfaI CTAG 5 cut(s) 183, 297, 539, 657, 887
BfmI CTRYAG 1 cut(s) 10
BisI GCNGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 140
BmcAI AGTACT 2 cut(s) 295, 514
Bme1390I CCNGG 3 cut(s) 341, 557, 591
BmgBI CACGTC 2 cut(s) 715, 931
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 3 cut(s) 341, 557, 591
BmsI GCATC 1 cut(s) 586
BpiI GAAGAC 1 cut(s) 396
BpmI CTGGAG 3 cut(s) 246, 567, 612
Bpu10I CCTNAGC 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 489
BsaJI CCNNGG 2 cut(s) 556, 752
BsaWI WCCGGW 1 cut(s) 817
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 346, 887
Bse1I ACTGG 1 cut(s) 850
BseAI TCCGGA 1 cut(s) 817
BseBI CCWGG 3 cut(s) 341, 557, 591
BseDI CCNNGG 2 cut(s) 556, 752
BseGI GGATG 3 cut(s) 79, 115, 831
BseLI CCNNNNNNNGG 3 cut(s) 224, 346, 887
BseMII CTCAG 5 cut(s) 156, 417, 767, 873, 876
BseNI ACTGG 1 cut(s) 850
Bsh1236I CGCG 2 cut(s) 123, 175
BshFI GGCC 2 cut(s) 589, 757
BshNI GGYRCC 1 cut(s) 454
BsiSI CCGG 1 cut(s) 818
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 3 cut(s) 224, 346, 887
BsmAI GTCTC 3 cut(s) 485, 699, 804
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 2 cut(s) 589, 757
Bsp13I TCCGGA 1 cut(s) 817
Bsp143I GATC 5 cut(s) 6, 233, 266, 299, 583
Bsp68I TCGCGA 1 cut(s) 175
BspACI CCGC 3 cut(s) 123, 138, 660
BspANI GGCC 2 cut(s) 589, 757
BspCNI CTCAG 5 cut(s) 155, 418, 768, 874, 875
BspEI TCCGGA 1 cut(s) 817
BspFNI CGCG 2 cut(s) 123, 175
BspLI GGNNCC 1 cut(s) 456
BspPI GGATC 2 cut(s) 241, 274
BspT107I GGYRCC 1 cut(s) 454
BsrI ACTGG 1 cut(s) 850
BssECI CCNNGG 2 cut(s) 556, 752
BssMI GATC 5 cut(s) 6, 233, 266, 299, 583
BssT1I CCWWGG 1 cut(s) 752
Bst2UI CCWGG 3 cut(s) 341, 557, 591
Bst4CI ACNGT 2 cut(s) 695, 899
BstC8I GCNNGC 1 cut(s) 575
BstDEI CTNAG 6 cut(s) 142, 426, 735, 776, 862, 882
BstENI CCTNNNNNAGG 1 cut(s) 885
BstF5I GGATG 3 cut(s) 79, 115, 831
BstFNI CGCG 2 cut(s) 123, 175
BstKTI GATC 5 cut(s) 9, 236, 269, 302, 586
BstMAI GTCTC 3 cut(s) 485, 699, 804
BstMBI GATC 5 cut(s) 6, 233, 266, 299, 583
BstMWI GCNNNNNNNGC 1 cut(s) 736
BstNI CCWGG 3 cut(s) 341, 557, 591
BstNSI RCATGY 1 cut(s) 452
BstSCI CCNGG 3 cut(s) 339, 555, 589
BstSFI CTRYAG 1 cut(s) 10
BstUI CGCG 2 cut(s) 123, 175
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 1 cut(s) 266
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 2 cut(s) 589, 757
BtrI CACGTC 2 cut(s) 715, 931
BtsCI GGATG 3 cut(s) 79, 115, 831
BtsIMutI CAGTG 1 cut(s) 309
BtuMI TCGCGA 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 575
CaiI CAGNNNCTG 1 cut(s) 149
CsiI ACCWGGT 1 cut(s) 339
Csp6I GTAC 2 cut(s) 294, 513
CviAII CATG 2 cut(s) 362, 449
CviJI RGCY 8 cut(s) 141, 350, 589, 600, 680, 730, 757, 886
CviKI_1 RGCY 8 cut(s) 141, 350, 589, 600, 680, 730, 757, 886
CviQI GTAC 2 cut(s) 294, 513
DdeI CTNAG 6 cut(s) 142, 426, 735, 776, 862, 882
DpnI GATC 5 cut(s) 8, 235, 268, 301, 585
DpnII GATC 5 cut(s) 6, 233, 266, 299, 583
DrdI GACNNNNNNGTC 1 cut(s) 698
DseDI GACNNNNNNGTC 1 cut(s) 698
Eco130I CCWWGG 1 cut(s) 752
EcoNI CCTNNNNNAGG 1 cut(s) 885
EcoRII CCWGG 3 cut(s) 339, 555, 589
EcoT14I CCWWGG 1 cut(s) 752
ErhI CCWWGG 1 cut(s) 752
FaeI CATG 2 cut(s) 365, 452
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 361, 448
FauI CCCGC 1 cut(s) 653
FblI GTMKAC 2 cut(s) 96, 369
Fnu4HI GCNGC 1 cut(s) 139
FokI GGATG 3 cut(s) 86, 122, 838
Fsp4HI GCNGC 1 cut(s) 139
FspBI CTAG 5 cut(s) 183, 297, 539, 657, 887
GluI GCNGC 1 cut(s) 139
GsuI CTGGAG 3 cut(s) 246, 567, 612
HaeIII GGCC 2 cut(s) 589, 757
HapII CCGG 1 cut(s) 818
Hin1II CATG 2 cut(s) 365, 452
HinfI GANTC 7 cut(s) 146, 502, 552, 594, 699, 763, 778
HpaII CCGG 1 cut(s) 818
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 6 cut(s) 97, 370, 563, 670, 791, 835
Hpy188I TCNGA 8 cut(s) 145, 151, 287, 427, 463, 704, 777, 865
Hpy188III TCNNGA 8 cut(s) 174, 225, 444, 474, 581, 782, 818, 881
Hpy8I GTNNAC 6 cut(s) 97, 370, 563, 670, 791, 835
HpyAV CCTTC 3 cut(s) 224, 235, 815
HpyCH4III ACNGT 2 cut(s) 695, 899
HpyCH4IV ACGT 2 cut(s) 714, 930
HpyCH4V TGCA 6 cut(s) 249, 417, 573, 623, 926, 940
HpyF10VI GCNNNNNNNGC 1 cut(s) 736
HpyF3I CTNAG 6 cut(s) 142, 426, 735, 776, 862, 882
HpySE526I ACGT 2 cut(s) 714, 930
Hsp92II CATG 2 cut(s) 365, 452
Kpn2I TCCGGA 1 cut(s) 817
Kzo9I GATC 5 cut(s) 6, 233, 266, 299, 583
LweI GCATC 1 cut(s) 586
MabI ACCWGGT 1 cut(s) 339
MaeI CTAG 5 cut(s) 183, 297, 539, 657, 887
MaeII ACGT 2 cut(s) 714, 930
MaeIII GTNAC 2 cut(s) 335, 378
MalI GATC 5 cut(s) 8, 235, 268, 301, 585
MboI GATC 5 cut(s) 6, 233, 266, 299, 583
MboII GAAGA 4 cut(s) 52, 101, 311, 396
MflI RGATCY 1 cut(s) 266
MluCI AATT 4 cut(s) 116, 202, 220, 722
MlyI GAGTC 4 cut(s) 496, 603, 708, 787
MmeI TCCRAC 1 cut(s) 787
MnlI CCTC 6 cut(s) 64, 421, 481, 492, 542, 691
MroI TCCGGA 1 cut(s) 817
MseI TTAA 1 cut(s) 410
MspI CCGG 1 cut(s) 818
MspR9I CCNGG 3 cut(s) 341, 557, 591
MvaI CCWGG 3 cut(s) 341, 557, 591
MvnI CGCG 2 cut(s) 123, 175
MwoI GCNNNNNNNGC 1 cut(s) 736
NdeII GATC 5 cut(s) 6, 233, 266, 299, 583
NlaIII CATG 2 cut(s) 365, 452
NlaIV GGNNCC 1 cut(s) 456
NmuCI GTSAC 1 cut(s) 378
NruI TCGCGA 1 cut(s) 175
NspI RCATGY 1 cut(s) 452
PciI ACATGT 1 cut(s) 448
PfeI GAWTC 3 cut(s) 146, 552, 763
PkrI GCNGC 1 cut(s) 140
PleI GAGTC 4 cut(s) 496, 602, 707, 786
PpsI GAGTC 4 cut(s) 496, 602, 707, 786
PscI ACATGT 1 cut(s) 448
Psp6I CCWGG 3 cut(s) 339, 555, 589
PspGI CCWGG 3 cut(s) 339, 555, 589
PspN4I GGNNCC 1 cut(s) 456
PstNI CAGNNNCTG 1 cut(s) 149
PsuI RGATCY 1 cut(s) 266
RruI TCGCGA 1 cut(s) 175
RsaI GTAC 2 cut(s) 295, 514
RsaNI GTAC 2 cut(s) 294, 513
SaqAI TTAA 1 cut(s) 410
SatI GCNGC 1 cut(s) 139
Sau3AI GATC 5 cut(s) 6, 233, 266, 299, 583
ScaI AGTACT 2 cut(s) 295, 514
SchI GAGTC 4 cut(s) 496, 603, 708, 787
ScrFI CCNGG 3 cut(s) 341, 557, 591
SexAI ACCWGGT 1 cut(s) 339
SfaNI GCATC 1 cut(s) 586
SfcI CTRYAG 1 cut(s) 10
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
Sse9I AATT 4 cut(s) 116, 202, 220, 722
SsiI CCGC 3 cut(s) 123, 138, 660
SspMI CTAG 5 cut(s) 183, 297, 539, 657, 887
StyD4I CCNGG 3 cut(s) 339, 555, 589
StyI CCWWGG 1 cut(s) 752
TaaI ACNGT 2 cut(s) 695, 899
TaiI ACGT 2 cut(s) 717, 933
TasI AATT 4 cut(s) 116, 202, 220, 722
TatI WGTACW 2 cut(s) 293, 512
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 3 cut(s) 146, 552, 763
Tru1I TTAA 1 cut(s) 410
Tru9I TTAA 1 cut(s) 410
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 1 cut(s) 378
Tsp45I GTSAC 1 cut(s) 378
TspDTI ATGAA 6 cut(s) 90, 129, 174, 311, 386, 396
TspGWI ACGGA 1 cut(s) 734
TspRI CASTG 1 cut(s) 309
XagI CCTNNNNNAGG 1 cut(s) 885
XapI RAATTY 2 cut(s) 116, 722
XceI RCATGY 1 cut(s) 452
XmiI GTMKAC 2 cut(s) 96, 369
XspI CTAG 5 cut(s) 183, 297, 539, 657, 887
ZrmI AGTACT 2 cut(s) 295, 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.