AT3G62240

E3 ubiquitin-protein ligase hel2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000854
Physical Location & Seq
Reverse (-)
23033355 .. 23036808
3454 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62240.1

Sequence Viewer

Length: 2439 bp
ATGGATGATAGCTGTGCCGTCTGCGCTGACAATCTCGAATGGGTTGCCTATGGATCTTGTGGTCACCGAGAGGTTTGCTCCACATGCGTCGTTCGTCTCCGATTCGTCCTCGACGATCCTCGCTGCTGTATCTGCAAGACCGAGTCTCCTATCGTTTTTGTCACCAAGGCATTGGGTGATTATACAAGGACGATCAATGATTTCTCCACCTTCCCATCAGCACCAAGGGAAGGTCGAGTAGGAGCTTTCTGGTATCATGAAGACACTCAGGCTTTCTTTGATGACTTGGATCAGTACAGGATGATCAAAGCTATGTGTAGACTTTCTTGCGGTGTTTGTGATAAGACGGAAGATAAACCTAGAGAAGGAGGACCAAGACATCATCGTCAGCGGATTAAGAGCGTCGAACAGTTGAAGGGTCATTTATATCATAAGCACAAATTGCATATGTGTGGTTTATGTCTAGAAGGGCGAAAGATATTCATATGTGAACAAAAGTTGTATACTAGGGCACAACTGAACCAACACATACAGACTGGTGACTCTGAAGTTGATGGAAGTGAGAGTGAAAGGGGAGGCTTCGCAGGACATCCTATGTGTGAATTCTGTAGGAATCCTTTCTATGGTGATAATGAATTGTATACTCACATGTCTACAGAGCATTACACCTGTCATCTATGCCAAAGGTCACAGCCAGGGCAATATGAATATTACAAAAACTATGATGACTTAGAGATTCATTTCCGTAGAGACCATTTCCTCTGTGAAGACGACTCCTGCCTTGCTAAGAAGTTCACAGTTTTCCAGAATGAATCAGAGTTGAAAAGGCATAACGCTATTGAGCATGGAGGTAAAATGTCTCGGTCTCAGCGAAGTGCTGCATTACAGATACCGACAAGTTTCCGATACAGTCGTGGAAATGATCAAGAGAATCGTCGAGGAAGACCCCGATCATTTCGTCGCGAGCCTGGTGATGAAGAATATAATCTTGCAGTTCATGCCGCTCTTCGTTTATCTGAGTCTGAGTATTCTCGGCAAGAGCCTGCTCCTCCACCCTCAAGTGCACCACCCGGATTCTCTGAAAACAACAACATACACGTGGATGATACTGATCCCCTTATTCAGCCAATGGAATCTCTATCAACTACAGATATGGAACCCTCTTCACGGTATCTTCAAGCTGTAGGAAGCTTTGGTGGTGGTGGTTCACGTCTTGGAGAATCTGCTTTTCCTCCTCTTTCTGGGCAACAGAGTTCTGGACAGAACGTAGAGAGTTTACCAACTAACACCATGGCTGCTCGTCTAAGACGCCAAACAAATCGAACTTCTACTGCTTCCGCTATTGCTAGTCCTTCTCAAGGATGGCCAGTGATTAATCGAGGCCCAGGACAAGCATCTATTACAAGTGGAGGTAATCATTCTTCTTCAGGATGGCCAGCTATAGGTCGAACGCCTGTACAAGCCTCTAGTTCTTCAGTCCAATCTAGGTCCCATAACAGAGTTTCACAACCTCGACCTCTGGCTTCTGCAGTTCCACAGGCTGCTCGTAATGCAAATAGGATTCCTCACTCTTCCTCAGCCCCTAATCTATCTGATACAAGATCACTTCAGCCTTCTCATTCAGATTTTCCTCCAGTTTCTTCTGCTGTTGTGCAAAATCGTAAAACATCATCAACCACTACACAGGGATCGTCAAACACTCAACCCCCTCCAGATGTACAATCTGCCAACAAGTCATTGATTGAGAAAATGCGATCTGCACTTGGTCATGATGAAGATGTATTCGTGGCCTTCAGGAATATATCAGGACAGTATCGTCAAGGTTCCATTGATGCAAAGACTTATTTGGAGTATGTGCAAGGTTATGGATTGTCTCACTTGGTTATTGATCTTGCTAGACTGTGTCCTGACCCTAAGAGGCAGAAGGAACTCATTGATACCCATAATGCCAGTTTGAGAGAAGAGGATTCAAAGGATAATGGTCGGAGTGCTGCGCAGAGCTCTTCTCAGCCTAAAGAGAGTCAAAGTTCAAAGAAAAACAAAGGAAAAGCTGTGAAAGTTGTTGACCCTAAAGAAACACTAGCAGATAACTTTATGGACACAGTAAGGAGATTACAATCTTCTCAGAATCCACAGGAAGAAGAAGAGGAGGCGATATCAAAGGATAAGAATACCTATAGATCAGACAAAGGCAAATCACAAGTAGTAGGAACGGATTCTTCTTCCACAGGAAGCAAACAGCAACGTAAGAAAACTTCTAAGTTTCACAGAGTACGGCTTGGAGATGGATCAATGGCTGCCTTGCTGGATCTTAACAATTCTACTCGCGAGTCTGAACAAGAATCTAAGGATAGTAATTCCAACAGTAACCAAAATCAGACCGGTGGTTTACCGGTTCGTGGGGTTTGGCGTAAAGGAGGTGCTAACCTTTTCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

812

Amino Acids

90.22

Weight (kDa)

8.43

Isoelectric Point (pI)

60.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RING_ZNF598 PF25447 2 - 57 8.3e-06 ZNF598 RING finger domain
zf-C3HC4_3 PF13920 2 - 48 3.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-C2H2_13 PF23230 221 - 277 1.4e-17 C2H2-type zinc finger
PAH_ZNF598 PF23202 574 - 650 8e-21 ZNF598 PAH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 384, 1815
Acc16I TGCGCA 1 cut(s) 1995
AccBSI CCGCTC 1 cut(s) 1004
AccI GTMKAC 4 cut(s) 319, 503, 641, 653
AccII CGCG 2 cut(s) 963, 2328
AciI CCGC 4 cut(s) 330, 391, 1002, 1338
AclWI GGATC 7 cut(s) 61, 110, 297, 1106, 1696, 2296, 2316
AcoI YGGCCR 2 cut(s) 1364, 1433
AcsI RAATTY 1 cut(s) 602
AcuI CTGAAG 5 cut(s) 567, 1410, 1458, 1592, 1777
AcvI CACGTG 1 cut(s) 1099
AcyI GRCGYC 1 cut(s) 1309
AfaI GTAC 4 cut(s) 296, 1458, 1719, 2274
AfiI CCNNNNNNNGG 8 cut(s) 230, 365, 623, 1167, 1241, 1358, 1442, 2399
AflIII ACRYGT 2 cut(s) 648, 1096
AgeI ACCGGT 2 cut(s) 2381, 2392
AgsI TTSAA 5 cut(s) 415, 823, 1178, 1971, 2031
AjiI CACGTC 1 cut(s) 1211
AjnI CCWGG 3 cut(s) 694, 967, 1384
AluBI AGCT 8 cut(s) 12, 245, 311, 1181, 1191, 1439, 2001, 2051
AluI AGCT 8 cut(s) 12, 245, 311, 1181, 1191, 1439, 2001, 2051
Alw21I GWGCWC 2 cut(s) 1066, 2003
Alw26I GTCTC 6 cut(s) 101, 150, 744, 864, 870, 1878
Alw44I GTGCAC 1 cut(s) 1062
AlwI GGATC 7 cut(s) 61, 110, 297, 1106, 1696, 2296, 2316
AoxI GGCC 4 cut(s) 1364, 1381, 1433, 1788
ApaLI GTGCAC 1 cut(s) 1062
ApeKI GCWGC 6 cut(s) 123, 878, 1295, 1541, 1991, 2297
ApoI RAATTY 1 cut(s) 602
AseI ATTAAT 1 cut(s) 1374
AsiGI ACCGGT 2 cut(s) 2381, 2392
Asp700I GAANNNNTTC 2 cut(s) 617, 2215
AspLEI GCGC 2 cut(s) 26, 1996
AspS9I GGNCC 3 cut(s) 371, 1382, 1488
AsuC2I CCSGG 1 cut(s) 1071
AsuHPI GGTGA 6 cut(s) 56, 154, 188, 551, 638, 983
AvaII GGWCC 2 cut(s) 371, 1488
BaeGI GKGCMC 2 cut(s) 514, 1066
BaeI ACNNNNGTAYC 2 cut(s) 898, 931
BalI TGGCCA 2 cut(s) 1366, 1435
BanII GRGCYC 1 cut(s) 2003
BarI GAAGNNNNNNTAC 4 cut(s) 1405, 1437, 2239, 2271
BbrPI CACGTG 1 cut(s) 1099
BbsI GAAGAC 3 cut(s) 267, 774, 949
Bbv12I GWGCWC 2 cut(s) 1066, 2003
BbvCI CCTCAGC 1 cut(s) 1576
BbvI GCAGC 6 cut(s) 110, 865, 1282, 1528, 1978, 2284
BccI CCATC 5 cut(s) 223, 548, 1356, 1425, 2279
BceAI ACGGC 2 cut(s) 2, 2291
BcgI CGANNNNNNTGC 4 cut(s) 26, 57, 60, 91
BciT130I CCWGG 3 cut(s) 696, 969, 1386
BclI TGATCA 2 cut(s) 303, 922
BcnI CCSGG 1 cut(s) 1071
BcoDI GTCTC 6 cut(s) 101, 150, 744, 864, 870, 1878
BfaI CTAG 8 cut(s) 360, 464, 507, 1347, 1467, 1485, 1896, 2081
BfmI CTRYAG 7 cut(s) 607, 654, 1146, 1182, 1440, 1527, 2176
BisI GCNGC 7 cut(s) 124, 879, 1002, 1296, 1542, 1992, 2298
BlsI GCNGC 7 cut(s) 125, 880, 1003, 1297, 1543, 1993, 2299
Bme1390I CCNGG 4 cut(s) 696, 969, 1071, 1386
Bme18I GGWCC 2 cut(s) 371, 1488
BmgBI CACGTC 1 cut(s) 1211
BmgT120I GGNCC 3 cut(s) 371, 1382, 1488
BmiI GGNNCC 3 cut(s) 1158, 1490, 1825
BmrFI CCNGG 4 cut(s) 696, 969, 1071, 1386
BmsI GCATC 2 cut(s) 1403, 1822
BpiI GAAGAC 3 cut(s) 267, 774, 949
BplI GAGNNNNNCTC 4 cut(s) 62, 94, 1990, 2022
BpmI CTGGAG 2 cut(s) 1617, 1695
Bpu10I CCTNAGC 1 cut(s) 1576
BpuEI CTTGAG 2 cut(s) 1042, 1341
BpuMI CCSGG 1 cut(s) 1071
BsaAI YACGTR 1 cut(s) 1099
BsaBI GATNNNNATC 2 cut(s) 1110, 2116
BsaHI GRCGYC 1 cut(s) 1309
BsaI GGTCTC 2 cut(s) 744, 870
BsaJI CCNNGG 5 cut(s) 165, 224, 695, 1290, 1384
BsaWI WCCGGW 2 cut(s) 2381, 2392
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 8 cut(s) 230, 365, 623, 1167, 1241, 1358, 1442, 2399
Bse118I RCCGGY 2 cut(s) 2381, 2392
Bse1I ACTGG 4 cut(s) 541, 1367, 1634, 1950
Bse8I GATNNNNATC 2 cut(s) 1110, 2116
BseBI CCWGG 3 cut(s) 696, 969, 1386
BseDI CCNNGG 5 cut(s) 165, 224, 695, 1290, 1384
BseGI GGATG 6 cut(s) 10, 306, 589, 1108, 1367, 1436
BseJI GATNNNNATC 2 cut(s) 1110, 2116
BseLI CCNNNNNNNGG 8 cut(s) 230, 365, 623, 1167, 1241, 1358, 1442, 2399
BseMII CTCAG 7 cut(s) 281, 881, 1008, 1014, 1590, 2021, 2138
BseNI ACTGG 4 cut(s) 541, 1367, 1634, 1950
BseRI GAGGAG 3 cut(s) 1038, 1224, 2162
BseSI GKGCMC 2 cut(s) 514, 1066
BseXI GCAGC 6 cut(s) 110, 865, 1282, 1528, 1978, 2284
BsgI GTGCAG 1 cut(s) 1743
Bsh1236I CGCG 2 cut(s) 963, 2328
BshFI GGCC 4 cut(s) 1366, 1383, 1435, 1790
BshTI ACCGGT 2 cut(s) 2381, 2392
BsiHKAI GWGCWC 2 cut(s) 1066, 2003
BsiSI CCGG 3 cut(s) 1071, 2382, 2393
BslFI GGGAC 1 cut(s) 1474
BslI CCNNNNNNNGG 8 cut(s) 230, 365, 623, 1167, 1241, 1358, 1442, 2399
BsmAI GTCTC 6 cut(s) 101, 150, 744, 864, 870, 1878
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 1474
BsnI GGCC 4 cut(s) 1366, 1383, 1435, 1790
Bso31I GGTCTC 2 cut(s) 744, 870
Bsp1286I GDGCHC 3 cut(s) 514, 1066, 2003
Bsp1407I TGTACA 2 cut(s) 1456, 1717
Bsp19I CCATGG 1 cut(s) 1290
Bsp68I TCGCGA 2 cut(s) 963, 2328
BspACI CCGC 4 cut(s) 330, 391, 1002, 1338
BspANI GGCC 4 cut(s) 1366, 1383, 1435, 1790
BspCNI CTCAG 7 cut(s) 280, 880, 1009, 1015, 1589, 2020, 2137
BspFNI CGCG 2 cut(s) 963, 2328
BspHI TCATGA 2 cut(s) 256, 1768
BspLI GGNNCC 3 cut(s) 1158, 1490, 1825
BspMAI CTGCAG 1 cut(s) 1531
BspPI GGATC 7 cut(s) 61, 110, 297, 1106, 1696, 2296, 2316
BspQI GCTCTTC 2 cut(s) 1011, 2008
BspTNI GGTCTC 2 cut(s) 744, 870
BsrBI CCGCTC 1 cut(s) 1004
BsrFI RCCGGY 2 cut(s) 2381, 2392
BsrGI TGTACA 2 cut(s) 1456, 1717
BsrI ACTGG 4 cut(s) 541, 1367, 1634, 1950
BssAI RCCGGY 2 cut(s) 2381, 2392
BssECI CCNNGG 5 cut(s) 165, 224, 695, 1290, 1384
BssNAI GTATAC 2 cut(s) 504, 642
BssNI GRCGYC 1 cut(s) 1309
BssT1I CCWWGG 3 cut(s) 165, 224, 1290
Bst1107I GTATAC 2 cut(s) 504, 642
Bst2UI CCWGG 3 cut(s) 696, 969, 1386
Bst4CI ACNGT 8 cut(s) 411, 799, 911, 1170, 1812, 1902, 2104, 2366
Bst6I CTCTTC 6 cut(s) 1011, 1168, 1576, 1956, 2008, 2139
BstACI GRCGYC 1 cut(s) 1309
BstAPI GCANNNNNTGC 2 cut(s) 442, 998
BstAUI TGTACA 2 cut(s) 1456, 1717
BstBAI YACGTR 1 cut(s) 1099
BstC8I GCNNGC 3 cut(s) 965, 1044, 1437
BstDSI CCRYGG 1 cut(s) 1290
BstEII GGTNACC 1 cut(s) 62
BstENI CCTNNNNNAGG 2 cut(s) 363, 1356
BstF5I GGATG 6 cut(s) 10, 306, 589, 1108, 1367, 1436
BstFNI CGCG 2 cut(s) 963, 2328
BstHHI GCGC 2 cut(s) 26, 1996
BstMAI GTCTC 6 cut(s) 101, 150, 744, 864, 870, 1878
BstMWI GCNNNNNNNGC 6 cut(s) 23, 84, 132, 442, 998, 1550
BstNI CCWGG 3 cut(s) 696, 969, 1386
BstNSI RCATGY 2 cut(s) 87, 652
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 4 cut(s) 694, 967, 1069, 1384
BstSFI CTRYAG 7 cut(s) 607, 654, 1146, 1182, 1440, 1527, 2176
BstSLI GKGCMC 2 cut(s) 514, 1066
BstUI CGCG 2 cut(s) 963, 2328
BstV1I GCAGC 6 cut(s) 110, 865, 1282, 1528, 1978, 2284
BstV2I GAAGAC 3 cut(s) 267, 774, 949
BstX2I RGATCY 2 cut(s) 53, 2308
BstXI CCANNNNNNTGG 1 cut(s) 172
BstYI RGATCY 2 cut(s) 53, 2308
BstZ17I GTATAC 2 cut(s) 504, 642
BsuRI GGCC 4 cut(s) 1366, 1383, 1435, 1790
BtgI CCRYGG 1 cut(s) 1290
BtrI CACGTC 1 cut(s) 1211
BtsCI GGATG 6 cut(s) 10, 306, 589, 1108, 1367, 1436
BtsIMutI CAGTG 1 cut(s) 1374
BtuMI TCGCGA 2 cut(s) 963, 2328
Cac8I GCNNGC 3 cut(s) 965, 1044, 1437
CciI TCATGA 2 cut(s) 256, 1768
CfoI GCGC 2 cut(s) 26, 1996
Cfr10I RCCGGY 2 cut(s) 2381, 2392
Cfr13I GGNCC 3 cut(s) 371, 1382, 1488
CseI GACGC 3 cut(s) 76, 391, 1317
Csp6I GTAC 4 cut(s) 295, 1457, 1718, 2273
CspAI ACCGGT 2 cut(s) 2381, 2392
CviAII CATG 7 cut(s) 84, 257, 649, 845, 998, 1291, 1769
CviQI GTAC 4 cut(s) 295, 1457, 1718, 2273
DrdI GACNNNNNNGTC 2 cut(s) 384, 1815
DseDI GACNNNNNNGTC 2 cut(s) 384, 1815
EaeI YGGCCR 2 cut(s) 1364, 1433
Eam1104I CTCTTC 6 cut(s) 1011, 1168, 1576, 1956, 2008, 2139
EarI CTCTTC 6 cut(s) 1011, 1168, 1576, 1956, 2008, 2139
Ecl136II GAGCTC 1 cut(s) 2001
Eco130I CCWWGG 3 cut(s) 165, 224, 1290
Eco24I GRGCYC 1 cut(s) 2003
Eco31I GGTCTC 2 cut(s) 744, 870
Eco32I GATATC 1 cut(s) 2157
Eco47I GGWCC 2 cut(s) 371, 1488
Eco53kI GAGCTC 1 cut(s) 2001
Eco57I CTGAAG 5 cut(s) 567, 1410, 1458, 1592, 1777
Eco72I CACGTG 1 cut(s) 1099
Eco91I GGTNACC 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 2001
EcoNI CCTNNNNNAGG 2 cut(s) 363, 1356
EcoO109I RGGNCCY 1 cut(s) 1488
EcoO65I GGTNACC 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 3 cut(s) 694, 967, 1384
EcoRV GATATC 1 cut(s) 2157
EcoT14I CCWWGG 3 cut(s) 165, 224, 1290
EcoT38I GRGCYC 1 cut(s) 2003
ErhI CCWWGG 3 cut(s) 165, 224, 1290
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 7 cut(s) 87, 260, 652, 848, 1001, 1294, 1772
FaqI GGGAC 1 cut(s) 1474
FatI CATG 7 cut(s) 83, 256, 648, 844, 997, 1290, 1768
FauNDI CATATG 2 cut(s) 447, 485
FbaI TGATCA 2 cut(s) 303, 922
FblI GTMKAC 4 cut(s) 319, 503, 641, 653
Fnu4HI GCNGC 7 cut(s) 124, 879, 1002, 1296, 1542, 1992, 2298
FokI GGATG 6 cut(s) 17, 313, 576, 1115, 1374, 1443
FriOI GRGCYC 1 cut(s) 2003
Fsp4HI GCNGC 7 cut(s) 124, 879, 1002, 1296, 1542, 1992, 2298
FspBI CTAG 8 cut(s) 360, 464, 507, 1347, 1467, 1485, 1896, 2081
FspI TGCGCA 1 cut(s) 1995
GlaI GCGC 2 cut(s) 25, 1995
GluI GCNGC 7 cut(s) 124, 879, 1002, 1296, 1542, 1992, 2298
GsuI CTGGAG 2 cut(s) 1617, 1695
HaeIII GGCC 4 cut(s) 1366, 1383, 1435, 1790
HapII CCGG 3 cut(s) 1071, 2382, 2393
HgaI GACGC 3 cut(s) 76, 391, 1317
HhaI GCGC 2 cut(s) 26, 1996
Hin1I GRCGYC 1 cut(s) 1309
Hin1II CATG 7 cut(s) 87, 260, 652, 848, 1001, 1294, 1772
Hin6I GCGC 2 cut(s) 24, 1994
HinP1I GCGC 2 cut(s) 24, 1994
HincII GTYRAC 1 cut(s) 2065
HindII GTYRAC 1 cut(s) 2065
HindIII AAGCTT 1 cut(s) 1189
HpaII CCGG 3 cut(s) 1071, 2382, 2393
HphI GGTGA 6 cut(s) 56, 154, 188, 551, 638, 983
Hpy99I CGWCG 5 cut(s) 92, 116, 407, 939, 963
HpyAV CCTTC 9 cut(s) 220, 224, 359, 409, 461, 1362, 1623, 1801, 1918
HpyCH4III ACNGT 8 cut(s) 411, 799, 911, 1170, 1812, 1902, 2104, 2366
HpyCH4IV ACGT 4 cut(s) 1098, 1210, 1266, 2245
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 84, 132, 442, 998, 1550
HpySE526I ACGT 4 cut(s) 1098, 1210, 1266, 2245
Hsp92I GRCGYC 1 cut(s) 1309
Hsp92II CATG 7 cut(s) 87, 260, 652, 848, 1001, 1294, 1772
HspAI GCGC 2 cut(s) 24, 1994
Ksp22I TGATCA 2 cut(s) 303, 922
LguI GCTCTTC 2 cut(s) 1011, 2008
LmnI GCTCC 3 cut(s) 83, 242, 1051
Lsp1109I GCAGC 6 cut(s) 110, 865, 1282, 1528, 1978, 2284
LweI GCATC 2 cut(s) 1403, 1822
MaeI CTAG 8 cut(s) 360, 464, 507, 1347, 1467, 1485, 1896, 2081
MaeII ACGT 4 cut(s) 1098, 1210, 1266, 2245
MaeIII GTNAC 5 cut(s) 62, 160, 539, 687, 2366
MbiI CCGCTC 1 cut(s) 1004
MflI RGATCY 2 cut(s) 53, 2308
MhlI GDGCHC 3 cut(s) 514, 1066, 2003
MlsI TGGCCA 2 cut(s) 1366, 1435
MluCI AATT 5 cut(s) 440, 602, 635, 2317, 2356
MluNI TGGCCA 2 cut(s) 1366, 1435
MlyI GAGTC 6 cut(s) 152, 536, 767, 1028, 2029, 2339
MmeI TCCRAC 2 cut(s) 1964, 2385
Mox20I TGGCCA 2 cut(s) 1366, 1435
MroXI GAANNNNTTC 2 cut(s) 617, 2215
MscI TGGCCA 2 cut(s) 1366, 1435
MseI TTAA 3 cut(s) 396, 1374, 2313
MslI CAYNNNNRTG 3 cut(s) 450, 1097, 1101
Msp20I TGGCCA 2 cut(s) 1366, 1435
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 3 cut(s) 1071, 2382, 2393
MspR9I CCNGG 4 cut(s) 696, 969, 1071, 1386
MvaI CCWGG 3 cut(s) 696, 969, 1386
MvnI CGCG 2 cut(s) 963, 2328
MwoI GCNNNNNNNGC 6 cut(s) 23, 84, 132, 442, 998, 1550
NciI CCSGG 1 cut(s) 1071
NcoI CCATGG 1 cut(s) 1290
NdeI CATATG 2 cut(s) 447, 485
NlaIII CATG 7 cut(s) 87, 260, 652, 848, 1001, 1294, 1772
NlaIV GGNNCC 3 cut(s) 1158, 1490, 1825
NmeAIII GCCGAG 1 cut(s) 1012
NmuCI GTSAC 4 cut(s) 62, 160, 539, 687
NruI TCGCGA 2 cut(s) 963, 2328
NsbI TGCGCA 1 cut(s) 1995
NspI RCATGY 2 cut(s) 87, 652
PagI TCATGA 2 cut(s) 256, 1768
PciI ACATGT 1 cut(s) 648
PciSI GCTCTTC 2 cut(s) 1011, 2008
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 868
PdmI GAANNNNTTC 2 cut(s) 617, 2215
PflFI GACNNNGTC 2 cut(s) 142, 1902
PinAI ACCGGT 2 cut(s) 2381, 2392
PkrI GCNGC 7 cut(s) 125, 880, 1003, 1297, 1543, 1993, 2299
PleI GAGTC 6 cut(s) 151, 536, 767, 1027, 2028, 2338
PmaCI CACGTG 1 cut(s) 1099
PmlI CACGTG 1 cut(s) 1099
PpsI GAGTC 6 cut(s) 151, 536, 767, 1027, 2028, 2338
Ppu21I YACGTR 1 cut(s) 1099
PpuMI RGGWCCY 1 cut(s) 1488
PscI ACATGT 1 cut(s) 648
PshBI ATTAAT 1 cut(s) 1374
Psp124BI GAGCTC 1 cut(s) 2003
Psp5II RGGWCCY 1 cut(s) 1488
Psp6I CCWGG 3 cut(s) 694, 967, 1384
PspCI CACGTG 1 cut(s) 1099
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 3 cut(s) 694, 967, 1384
PspN4I GGNNCC 3 cut(s) 1158, 1490, 1825
PspPI GGNCC 3 cut(s) 371, 1382, 1488
PspPPI RGGWCCY 1 cut(s) 1488
PstI CTGCAG 1 cut(s) 1531
PsuI RGATCY 2 cut(s) 53, 2308
PsyI GACNNNGTC 2 cut(s) 142, 1902
RruI TCGCGA 2 cut(s) 963, 2328
RsaI GTAC 4 cut(s) 296, 1458, 1719, 2274
RsaNI GTAC 4 cut(s) 295, 1457, 1718, 2273
RseI CAYNNNNRTG 3 cut(s) 450, 1097, 1101
SacI GAGCTC 1 cut(s) 2003
SapI GCTCTTC 2 cut(s) 1011, 2008
SaqAI TTAA 3 cut(s) 396, 1374, 2313
SatI GCNGC 7 cut(s) 124, 879, 1002, 1296, 1542, 1992, 2298
Sau96I GGNCC 3 cut(s) 371, 1382, 1488
SchI GAGTC 6 cut(s) 152, 536, 767, 1028, 2029, 2339
ScrFI CCNGG 4 cut(s) 696, 969, 1071, 1386
SduI GDGCHC 3 cut(s) 514, 1066, 2003
SfaNI GCATC 2 cut(s) 1403, 1822
SfcI CTRYAG 7 cut(s) 607, 654, 1146, 1182, 1440, 1527, 2176
SinI GGWCC 2 cut(s) 371, 1488
SmiMI CAYNNNNRTG 3 cut(s) 450, 1097, 1101
SmlI CTYRAG 2 cut(s) 1057, 1356
SmoI CTYRAG 2 cut(s) 1057, 1356
Sse9I AATT 5 cut(s) 440, 602, 635, 2317, 2356
SsiI CCGC 4 cut(s) 330, 391, 1002, 1338
SspI AATATT 1 cut(s) 710
SspMI CTAG 8 cut(s) 360, 464, 507, 1347, 1467, 1485, 1896, 2081
SstI GAGCTC 1 cut(s) 2003
StyD4I CCNGG 4 cut(s) 694, 967, 1069, 1384
StyI CCWWGG 3 cut(s) 165, 224, 1290
TaaI ACNGT 8 cut(s) 411, 799, 911, 1170, 1812, 1902, 2104, 2366
TaiI ACGT 4 cut(s) 1101, 1213, 1269, 2248
TaqI TCGA 9 cut(s) 36, 111, 235, 405, 937, 1321, 1378, 1447, 1513
TaqII GACCGA 2 cut(s) 155, 852
TasI AATT 5 cut(s) 440, 602, 635, 2317, 2356
TatI WGTACW 3 cut(s) 294, 1456, 1717
TauI GCSGC 1 cut(s) 1004
Tru1I TTAA 3 cut(s) 396, 1374, 2313
Tru9I TTAA 3 cut(s) 396, 1374, 2313
TscAI CASTG 1 cut(s) 1374
TseFI GTSAC 4 cut(s) 62, 160, 539, 687
TseI GCWGC 6 cut(s) 123, 878, 1295, 1541, 1991, 2297
Tsp45I GTSAC 4 cut(s) 62, 160, 539, 687
TspDTI ATGAA 9 cut(s) 273, 472, 648, 720, 728, 825, 986, 990, 1788
TspGWI ACGGA 3 cut(s) 362, 734, 2228
TspRI CASTG 1 cut(s) 1374
Tth111I GACNNNGTC 2 cut(s) 142, 1902
VneI GTGCAC 1 cut(s) 1062
VpaK11BI GGWCC 2 cut(s) 371, 1488
VspI ATTAAT 1 cut(s) 1374
XagI CCTNNNNNAGG 2 cut(s) 363, 1356
XapI RAATTY 1 cut(s) 602
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 2 cut(s) 87, 652
XmiI GTMKAC 4 cut(s) 319, 503, 641, 653
XmnI GAANNNNTTC 2 cut(s) 617, 2215
XspI CTAG 8 cut(s) 360, 464, 507, 1347, 1467, 1485, 1896, 2081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.