pycom02g23760

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000854
Physical Location & Seq
Forward (+)
21896983 .. 21899103
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23760.4

Sequence Viewer

Length: 1014 bp
ATGGACGACAGCTGTGCGGTGTGTGCCGATACTCTGGAATGGGTTGCCTACGGTGCCTGTGGACATCGCGAAGTCTGCTCCACCTGCGTCGTTCGTCTCCGCTTCATCTGCAACGATCGCCGATGCTGCATCTGCAAGACCGAGTCCGACGTCATTTTTATCACCAAGGCTTTAGGAGATTATACAAGGATGATTAATGACTTCTCAGTCTTGCCGACTGAAGTAAGGGAGGGTCGCGTGGGACCATATTGGTACCACGAGGACACTCAAGCATTTTTCGATGATGTGGACCACTACAAGATGATCGATCTCAAATCCATACCAAGGCAATGTGCAAGCTTTCATGCAGCGAATGTGACAAGATGGATGAGCAATCAAATGATGGCCCAAAGCGTCGTGGGAAGATTCGGAACCTTGATCGATCTGAAGAATCATTTATTCCACCAACATAGGTTGTGGATGTGCTCTTTATGTTTGGAAGGGAGGAAGGTGTTTATATGTGAACAAAAACTATATACTAGAGCTCAATTGAGGCGGCATATAAATACAGGTGACTCTGAAGTGGATGGAAGTGAGAGTGAAAGAGGTGGCTTCATGGGACATCCTATGTGTGAGTTTTGCAGAACCCCATTTTATGGCGATAATGAGCTGTACTCCCACATGACTATCGATCACTATACTTGTCATATATGCCAAAGGCAGAATCCTGGACAATATGAATATTATAAGAATTATGATGACCTGGAGATGCACTTCCGCCAAGGTCATTTTCTATGTGAAGATGAGTCCTGCCTCAGCAAAAAGTTTGTTGTCTTCCAATCTGAGGCAGAAATGAAGAGGCATAATACACTTGAACATGGAGGGAGCATGTCTCGTTCCAAGCGTAATGCTGCTCTGCAGATACCAACCAGCTTCAGATATCAACGAAGTAGTGAACAAGATCGATCGACGTGGAAGAGGCGGAAGAGGTGGAAGAGGTGGAAGAGGTCTGGCATTTCATCGCGATTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

39.78

Weight (kDa)

8.46

Isoelectric Point (pI)

61.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AarI CACCTGC 1 cut(s) 92
AasI GACNNNNNNGTC 1 cut(s) 206
AatII GACGTC 1 cut(s) 153
Acc36I ACCTGC 1 cut(s) 92
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 2 cut(s) 53, 252
AccB7I CCANNNNNTGG 1 cut(s) 635
AccII CGCG 3 cut(s) 69, 237, 1003
AciI CCGC 5 cut(s) 17, 100, 535, 757, 961
AcuI CTGAAG 4 cut(s) 240, 446, 579, 898
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 2 cut(s) 254, 653
AfiI CCNNNNNNNGG 3 cut(s) 324, 635, 823
AgsI TTSAA 1 cut(s) 854
AjiI CACGTC 1 cut(s) 951
AjnI CCWGG 2 cut(s) 706, 741
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AluBI AGCT 5 cut(s) 12, 339, 524, 649, 912
AluI AGCT 5 cut(s) 12, 339, 524, 649, 912
Alw21I GWGCWC 2 cut(s) 467, 526
Alw26I GTCTC 2 cut(s) 101, 876
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 3 cut(s) 126, 347, 890
AseI ATTAAT 1 cut(s) 195
Asp718I GGTACC 1 cut(s) 252
AspS9I GGNCC 3 cut(s) 242, 289, 385
AsuHPI GGTGA 2 cut(s) 154, 563
AvaII GGWCC 2 cut(s) 242, 289
BanI GGYRCC 2 cut(s) 53, 252
BanII GRGCYC 1 cut(s) 526
BauI CACGAG 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 805
Bbv12I GWGCWC 2 cut(s) 467, 526
BbvCI CCTCAGC 1 cut(s) 794
BbvI GCAGC 3 cut(s) 113, 359, 877
BccI CCATC 3 cut(s) 357, 376, 560
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 708, 743
BcoDI GTCTC 2 cut(s) 101, 876
BfaI CTAG 1 cut(s) 519
BfmI CTRYAG 1 cut(s) 896
BfuAI ACCTGC 1 cut(s) 92
BisI GCNGC 4 cut(s) 127, 348, 536, 891
BlsI GCNGC 4 cut(s) 128, 349, 537, 892
Bme1390I CCNGG 2 cut(s) 708, 743
Bme18I GGWCC 2 cut(s) 242, 289
BmgBI CACGTC 1 cut(s) 951
BmgT120I GGNCC 3 cut(s) 242, 289, 385
BmiI GGNNCC 4 cut(s) 55, 243, 254, 412
BmrFI CCNGG 2 cut(s) 708, 743
BmsI GCATC 3 cut(s) 113, 138, 738
BpiI GAAGAC 1 cut(s) 805
BplI GAGNNNNNCTC 4 cut(s) 638, 670, 856, 888
BpmI CTGGAG 1 cut(s) 764
Bpu10I CCTNAGC 1 cut(s) 794
BpuEI CTTGAG 1 cut(s) 252
Bsa29I ATCGAT 4 cut(s) 306, 420, 669, 943
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 165, 323, 760
Bsc4I CCNNNNNNNGG 3 cut(s) 324, 635, 823
Bse3DI GCAATG 1 cut(s) 335
BseBI CCWGG 2 cut(s) 708, 743
BseCI ATCGAT 4 cut(s) 306, 420, 669, 943
BseDI CCNNGG 3 cut(s) 165, 323, 760
BseGI GGATG 5 cut(s) 195, 372, 465, 571, 601
BseLI CCNNNNNNNGG 3 cut(s) 324, 635, 823
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 3 cut(s) 219, 808, 813
BseXI GCAGC 3 cut(s) 113, 359, 877
Bsh1236I CGCG 3 cut(s) 69, 237, 1003
Bsh1285I CGRYCG 2 cut(s) 118, 947
BshFI GGCC 1 cut(s) 386
BshNI GGYRCC 2 cut(s) 53, 252
BshVI ATCGAT 4 cut(s) 306, 420, 669, 943
BsiEI CGRYCG 2 cut(s) 118, 947
BsiHKAI GWGCWC 2 cut(s) 467, 526
BslFI GGGAC 2 cut(s) 255, 612
BslI CCNNNNNNNGG 3 cut(s) 324, 635, 823
BsmAI GTCTC 2 cut(s) 101, 876
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 2 cut(s) 255, 612
BsnI GGCC 1 cut(s) 386
Bsp1286I GDGCHC 2 cut(s) 467, 526
Bsp143I GATC 8 cut(s) 115, 303, 307, 417, 421, 670, 940, 944
Bsp68I TCGCGA 2 cut(s) 69, 1003
BspACI CCGC 5 cut(s) 17, 100, 535, 757, 961
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 3 cut(s) 218, 807, 814
BspDI ATCGAT 4 cut(s) 306, 420, 669, 943
BspFNI CGCG 3 cut(s) 69, 237, 1003
BspLI GGNNCC 4 cut(s) 55, 243, 254, 412
BspMAI CTGCAG 1 cut(s) 900
BspMI ACCTGC 1 cut(s) 92
BspT107I GGYRCC 2 cut(s) 53, 252
BsrDI GCAATG 1 cut(s) 335
BssECI CCNNGG 3 cut(s) 165, 323, 760
BssMI GATC 8 cut(s) 115, 303, 307, 417, 421, 670, 940, 944
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 257
BssT1I CCWWGG 3 cut(s) 165, 323, 760
Bst2BI CACGAG 1 cut(s) 257
Bst2UI CCWGG 2 cut(s) 708, 743
Bst4CI ACNGT 1 cut(s) 53
Bst6I CTCTTC 5 cut(s) 830, 950, 959, 968, 977
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 3 cut(s) 205, 794, 822
BstF5I GGATG 5 cut(s) 195, 372, 465, 571, 601
BstFNI CGCG 3 cut(s) 69, 237, 1003
BstKTI GATC 8 cut(s) 118, 306, 310, 420, 424, 673, 943, 947
BstMAI GTCTC 2 cut(s) 101, 876
BstMBI GATC 8 cut(s) 115, 303, 307, 417, 421, 670, 940, 944
BstMCI CGRYCG 2 cut(s) 118, 947
BstMWI GCNNNNNNNGC 8 cut(s) 23, 53, 75, 84, 108, 117, 126, 132
BstNI CCWGG 2 cut(s) 708, 743
BstNSI RCATGY 1 cut(s) 871
BstSCI CCNGG 2 cut(s) 706, 741
BstSFI CTRYAG 1 cut(s) 896
BstUI CGCG 3 cut(s) 69, 237, 1003
BstV1I GCAGC 3 cut(s) 113, 359, 877
BstV2I GAAGAC 1 cut(s) 805
Bsu15I ATCGAT 4 cut(s) 306, 420, 669, 943
BsuRI GGCC 1 cut(s) 386
BsuTUI ATCGAT 4 cut(s) 306, 420, 669, 943
BtgZI GCGATG 2 cut(s) 50, 984
BtrI CACGTC 1 cut(s) 951
BtsCI GGATG 5 cut(s) 195, 372, 465, 571, 601
BtuMI TCGCGA 2 cut(s) 69, 1003
BveI ACCTGC 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 337
Cfr13I GGNCC 3 cut(s) 242, 289, 385
ClaI ATCGAT 4 cut(s) 306, 420, 669, 943
CseI GACGC 2 cut(s) 76, 382
Csp6I GTAC 2 cut(s) 253, 652
CviAII CATG 5 cut(s) 344, 595, 661, 857, 868
CviJI RGCY 8 cut(s) 12, 170, 339, 386, 524, 591, 649, 912
CviKI_1 RGCY 8 cut(s) 12, 170, 339, 386, 524, 591, 649, 912
CviQI GTAC 2 cut(s) 253, 652
DdeI CTNAG 3 cut(s) 205, 794, 822
DpnI GATC 8 cut(s) 117, 305, 309, 419, 423, 672, 942, 946
DpnII GATC 8 cut(s) 115, 303, 307, 417, 421, 670, 940, 944
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
Eam1104I CTCTTC 5 cut(s) 830, 950, 959, 968, 977
EarI CTCTTC 5 cut(s) 830, 950, 959, 968, 977
EciI GGCGGA 2 cut(s) 746, 976
Ecl136II GAGCTC 1 cut(s) 524
Eco130I CCWWGG 3 cut(s) 165, 323, 760
Eco24I GRGCYC 1 cut(s) 526
Eco32I GATATC 1 cut(s) 920
Eco47I GGWCC 2 cut(s) 242, 289
Eco53kI GAGCTC 1 cut(s) 524
Eco57I CTGAAG 4 cut(s) 240, 446, 579, 898
EcoICRI GAGCTC 1 cut(s) 524
EcoRII CCWGG 2 cut(s) 706, 741
EcoRV GATATC 1 cut(s) 920
EcoT14I CCWWGG 3 cut(s) 165, 323, 760
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 3 cut(s) 165, 323, 760
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 5 cut(s) 347, 598, 664, 860, 871
FaqI GGGAC 2 cut(s) 255, 612
FatI CATG 5 cut(s) 343, 594, 660, 856, 867
Fnu4HI GCNGC 4 cut(s) 127, 348, 536, 891
FokI GGATG 5 cut(s) 202, 379, 472, 578, 588
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 4 cut(s) 127, 348, 536, 891
FspBI CTAG 1 cut(s) 519
GluI GCNGC 4 cut(s) 127, 348, 536, 891
GsuI CTGGAG 1 cut(s) 764
HaeIII GGCC 1 cut(s) 386
HgaI GACGC 2 cut(s) 76, 382
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 5 cut(s) 347, 598, 664, 860, 871
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 7 cut(s) 143, 405, 430, 554, 703, 785, 1005
HphI GGTGA 2 cut(s) 154, 563
Hpy166II GTNNAC 4 cut(s) 62, 289, 503, 935
Hpy188I TCNGA 7 cut(s) 148, 410, 426, 559, 823, 917, 1013
Hpy188III TCNNGA 3 cut(s) 35, 68, 1002
Hpy8I GTNNAC 4 cut(s) 62, 289, 503, 935
Hpy99I CGWCG 4 cut(s) 92, 152, 398, 952
HpyAV CCTTC 2 cut(s) 473, 481
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4IV ACGT 2 cut(s) 150, 950
HpyCH4V TGCA 8 cut(s) 111, 129, 135, 335, 347, 621, 751, 898
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 53, 75, 84, 108, 117, 126, 132
HpyF3I CTNAG 3 cut(s) 205, 794, 822
HpySE526I ACGT 2 cut(s) 150, 950
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 5 cut(s) 347, 598, 664, 860, 871
KpnI GGTACC 1 cut(s) 256
Kzo9I GATC 8 cut(s) 115, 303, 307, 417, 421, 670, 940, 944
LmnI GCTCC 2 cut(s) 83, 864
Lsp1109I GCAGC 3 cut(s) 113, 359, 877
LweI GCATC 3 cut(s) 113, 138, 738
MaeI CTAG 1 cut(s) 519
MaeII ACGT 2 cut(s) 150, 950
MaeIII GTNAC 2 cut(s) 355, 551
MalI GATC 8 cut(s) 117, 305, 309, 419, 423, 672, 942, 946
MboI GATC 8 cut(s) 115, 303, 307, 417, 421, 670, 940, 944
MfeI CAATTG 1 cut(s) 527
MhlI GDGCHC 2 cut(s) 467, 526
MluCI AATT 2 cut(s) 527, 730
MlyI GAGTC 3 cut(s) 152, 548, 794
MmeI TCCRAC 1 cut(s) 171
MseI TTAA 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 12
MspR9I CCNGG 2 cut(s) 708, 743
MunI CAATTG 1 cut(s) 527
MvaI CCWGG 2 cut(s) 708, 743
MvnI CGCG 3 cut(s) 69, 237, 1003
MwoI GCNNNNNNNGC 8 cut(s) 23, 53, 75, 84, 108, 117, 126, 132
NdeII GATC 8 cut(s) 115, 303, 307, 417, 421, 670, 940, 944
NlaIII CATG 5 cut(s) 347, 598, 664, 860, 871
NlaIV GGNNCC 4 cut(s) 55, 243, 254, 412
NmuCI GTSAC 2 cut(s) 355, 551
NruI TCGCGA 2 cut(s) 69, 1003
NspI RCATGY 1 cut(s) 871
PaqCI CACCTGC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 880
PfeI GAWTC 4 cut(s) 405, 430, 703, 1005
PflFI GACNNNGTC 1 cut(s) 142
PflMI CCANNNNNTGG 1 cut(s) 635
PfoI TCCNGGA 1 cut(s) 706
PkrI GCNGC 4 cut(s) 128, 349, 537, 892
Ple19I CGATCG 2 cut(s) 118, 947
PleI GAGTC 3 cut(s) 151, 548, 793
PpsI GAGTC 3 cut(s) 151, 548, 793
PshBI ATTAAT 1 cut(s) 195
PsiI TTATAA 1 cut(s) 726
Psp124BI GAGCTC 1 cut(s) 526
Psp6I CCWGG 2 cut(s) 706, 741
PspGI CCWGG 2 cut(s) 706, 741
PspN4I GGNNCC 4 cut(s) 55, 243, 254, 412
PspPI GGNCC 3 cut(s) 242, 289, 385
PstI CTGCAG 1 cut(s) 900
PsyI GACNNNGTC 1 cut(s) 142
PvuI CGATCG 2 cut(s) 118, 947
PvuII CAGCTG 1 cut(s) 12
RruI TCGCGA 2 cut(s) 69, 1003
RsaI GTAC 2 cut(s) 254, 653
RsaNI GTAC 2 cut(s) 253, 652
SacI GAGCTC 1 cut(s) 526
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 4 cut(s) 127, 348, 536, 891
Sau3AI GATC 8 cut(s) 115, 303, 307, 417, 421, 670, 940, 944
Sau96I GGNCC 3 cut(s) 242, 289, 385
SchI GAGTC 3 cut(s) 152, 548, 794
ScrFI CCNGG 2 cut(s) 708, 743
SduI GDGCHC 2 cut(s) 467, 526
SfaNI GCATC 3 cut(s) 113, 138, 738
SfcI CTRYAG 1 cut(s) 896
SinI GGWCC 2 cut(s) 242, 289
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
Sse9I AATT 2 cut(s) 527, 730
SsiI CCGC 5 cut(s) 17, 100, 535, 757, 961
SspI AATATT 1 cut(s) 722
SspMI CTAG 1 cut(s) 519
SstI GAGCTC 1 cut(s) 526
StyD4I CCNGG 2 cut(s) 706, 741
StyI CCWWGG 3 cut(s) 165, 323, 760
TaaI ACNGT 1 cut(s) 53
TaiI ACGT 2 cut(s) 153, 953
TaqI TCGA 6 cut(s) 279, 306, 420, 669, 943, 947
TaqII GACCGA 1 cut(s) 155
TasI AATT 2 cut(s) 527, 730
TatI WGTACW 1 cut(s) 651
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 4 cut(s) 405, 430, 703, 1005
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseFI GTSAC 2 cut(s) 355, 551
TseI GCWGC 3 cut(s) 126, 347, 890
Tsp45I GTSAC 2 cut(s) 355, 551
TspDTI ATGAA 6 cut(s) 94, 332, 583, 732, 848, 987
Tth111I GACNNNGTC 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 635
VpaK11BI GGWCC 2 cut(s) 242, 289
VspI ATTAAT 1 cut(s) 195
XceI RCATGY 1 cut(s) 871
XspI CTAG 1 cut(s) 519
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.