pycom04g16640

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000854
Physical Location & Seq
Reverse (-)
19302213 .. 19302938
726 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g16640.1

Sequence Viewer

Length: 726 bp
ATGGCTTCTTGCATTTGTTGCCTTAACAAAGTAACTGTCCTTAATACTTCTCCAGTTTGGCCTGCAACAAATCGTCAACCTAAATCATCTGCCAGTAGTTGTCACCAGAAAAAGCCCTCTTCAGGCTCTGGGCACATATCATCATCTAGTTCTCCTGTATTTTCCCTGAGCGGTCCCCCTAAGCAAGCTGCAGCCACTGGGTTTGTTTCATCTAATTTTGCCAGTCCAAAGAATTCGACTGGAACCAGTAAGGTCGCCCAATCTGCTTCAGGTCCAAAAATTATCAATACCGGTTCTTTGGACTCCGATTTTCCTTCTGTTTCTGCTAATCAAACAAATAAAGAACCTGGTACTGCAAGTATTCAGAGAGTGCCAAAGATTGGAGATGTTCATGCTGTCAACAATTCTCTTGTGGAAAAAATCCGCGATGCTTTGGAACGTGATGACAACAAATTCTCCGCATTTAATGAAATATCTTCAGACTACCGCAAGGATATAATCAATACAGAGGAATATCTTGCTTATGTCTACCAGTTCGGCCTATCACATTTGCTTCTCGAGCTAGCTAGACTCTGTCCTGATGCTGAGAAACAAAGAGAACTTGTCGAGACTTACAATTTGAGCATAATGATATTCGATTCAGCTCAGCTCGTTTACAGCCTAAGAGGTGGTGTCGTTGGTGGCAATAATGACAGTAGTGTTGGGAACTGGTTCAGAAGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

25.9

Weight (kDa)

8.56

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAH_ZNF598 PF23202 134 - 206 1.3e-21 ZNF598 PAH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 380
AccBSI CCGCTC 1 cut(s) 171
AccI GTMKAC 1 cut(s) 528
AccII CGCG 1 cut(s) 426
AciI CCGC 4 cut(s) 171, 424, 459, 487
AcsI RAATTY 2 cut(s) 232, 452
AcuI CTGAAG 3 cut(s) 105, 252, 462
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 2 cut(s) 122, 380
AgeI ACCGGT 1 cut(s) 290
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 5 cut(s) 188, 562, 566, 644, 649
AluI AGCT 5 cut(s) 188, 562, 566, 644, 649
Alw26I GTCTC 1 cut(s) 602
AlwNI CAGNNNCTG 2 cut(s) 128, 197
Ama87I CYCGRG 1 cut(s) 557
AoxI GGCC 2 cut(s) 59, 538
ApeKI GCWGC 2 cut(s) 188, 191
ApoI RAATTY 2 cut(s) 232, 452
ArsI GACNNNNNNTTYG 4 cut(s) 21, 53, 601, 633
AsiGI ACCGGT 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 710
AspS9I GGNCC 2 cut(s) 173, 272
AsuHPI GGTGA 1 cut(s) 95
AsuNHI GCTAGC 1 cut(s) 562
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 2 cut(s) 173, 272
BaeGI GKGCMC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 175, 203
BciT130I CCWGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 3 cut(s) 147, 563, 567
BfmI CTRYAG 1 cut(s) 189
BglII AGATCT 1 cut(s) 719
BisI GCNGC 2 cut(s) 189, 192
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 2 cut(s) 190, 193
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 2 cut(s) 173, 272
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 2 cut(s) 173, 272
BmiI GGNNCC 2 cut(s) 175, 244
BmrFI CCNGG 1 cut(s) 348
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 2 cut(s) 418, 571
BmtI GCTAGC 1 cut(s) 566
BmuI ACTGGG 1 cut(s) 207
BpmI CTGGAG 1 cut(s) 36
Bpu10I CCTNAGC 2 cut(s) 167, 180
Bpu1102I GCTNAGC 1 cut(s) 645
BsaWI WCCGGW 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 380
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 8 cut(s) 53, 93, 202, 222, 244, 246, 532, 713
BseBI CCWGG 1 cut(s) 348
BseLI CCNNNNNNNGG 2 cut(s) 122, 380
BseMII CTCAG 3 cut(s) 158, 576, 659
BseNI ACTGG 8 cut(s) 53, 93, 202, 222, 244, 246, 532, 713
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 2 cut(s) 175, 203
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 2 cut(s) 61, 540
BshTI ACCGGT 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 122, 380
BsmAI GTCTC 1 cut(s) 602
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 2 cut(s) 61, 540
BsoBI CYCGRG 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 1 cut(s) 719
Bsp1720I GCTNAGC 1 cut(s) 645
BspACI CCGC 4 cut(s) 171, 424, 459, 487
BspANI GGCC 2 cut(s) 61, 540
BspCNI CTCAG 3 cut(s) 159, 577, 658
BspFNI CGCG 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 175, 244
BspMAI CTGCAG 1 cut(s) 193
BspOI GCTAGC 1 cut(s) 566
BsrBI CCGCTC 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 8 cut(s) 53, 93, 202, 222, 244, 246, 532, 713
BssAI RCCGGY 1 cut(s) 290
BssMI GATC 1 cut(s) 719
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 2 cut(s) 37, 695
Bst6I CTCTTC 1 cut(s) 124
BstAPI GCANNNNNTGC 1 cut(s) 18
BstC8I GCNNGC 3 cut(s) 63, 186, 564
BstDEI CTNAG 5 cut(s) 167, 180, 585, 645, 662
BstFNI CGCG 1 cut(s) 426
BstKTI GATC 1 cut(s) 722
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 1 cut(s) 719
BstMWI GCNNNNNNNGC 3 cut(s) 18, 263, 559
BstNI CCWGG 1 cut(s) 348
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 135
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 2 cut(s) 175, 203
BstX2I RGATCY 1 cut(s) 719
BstYI RGATCY 1 cut(s) 719
BsuRI GGCC 2 cut(s) 61, 540
BtgZI GCGATG 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 3 cut(s) 63, 186, 564
CaiI CAGNNNCTG 2 cut(s) 128, 197
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 2 cut(s) 173, 272
CsiI ACCWGGT 1 cut(s) 346
Csp6I GTAC 1 cut(s) 351
CspAI ACCGGT 1 cut(s) 290
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 1 cut(s) 392
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 5 cut(s) 167, 180, 585, 645, 662
DpnI GATC 1 cut(s) 721
DpnII GATC 1 cut(s) 719
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 173, 272
Eco57I CTGAAG 3 cut(s) 105, 252, 462
Eco88I CYCGRG 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 346
FaeI CATG 1 cut(s) 395
FaiI YATR 5 cut(s) 137, 393, 497, 525, 626
FaqI GGGAC 1 cut(s) 159
FatI CATG 1 cut(s) 391
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 2 cut(s) 189, 192
Fsp4HI GCNGC 2 cut(s) 189, 192
FspBI CTAG 3 cut(s) 147, 563, 567
GluI GCNGC 2 cut(s) 189, 192
GsuI CTGGAG 1 cut(s) 36
HaeIII GGCC 2 cut(s) 61, 540
HapII CCGG 1 cut(s) 291
Hin1II CATG 1 cut(s) 395
HincII GTYRAC 2 cut(s) 77, 400
HindII GTYRAC 2 cut(s) 77, 400
HinfI GANTC 3 cut(s) 302, 570, 638
HpaII CCGG 1 cut(s) 291
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 4 cut(s) 77, 400, 529, 655
Hpy188I TCNGA 4 cut(s) 307, 366, 481, 716
Hpy188III TCNNGA 4 cut(s) 557, 578, 607, 723
Hpy8I GTNNAC 4 cut(s) 77, 400, 529, 655
HpyAV CCTTC 1 cut(s) 324
HpyCH4III ACNGT 2 cut(s) 37, 695
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 4 cut(s) 12, 65, 191, 356
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 263, 559
HpyF3I CTNAG 5 cut(s) 167, 180, 585, 645, 662
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 1 cut(s) 395
Kzo9I GATC 1 cut(s) 719
Lsp1109I GCAGC 2 cut(s) 175, 203
LweI GCATC 2 cut(s) 418, 571
MabI ACCWGGT 1 cut(s) 346
MaeI CTAG 3 cut(s) 147, 563, 567
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 2 cut(s) 31, 101
MalI GATC 1 cut(s) 721
MbiI CCGCTC 1 cut(s) 171
MboI GATC 1 cut(s) 719
MboII GAAGA 2 cut(s) 111, 468
MflI RGATCY 1 cut(s) 719
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 6 cut(s) 214, 232, 279, 403, 452, 616
MlyI GAGTC 2 cut(s) 296, 564
MnlI CCTC 3 cut(s) 127, 502, 659
MroXI GAANNNNTTC 1 cut(s) 710
MseI TTAA 3 cut(s) 24, 42, 465
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 348
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 18, 263, 559
NdeII GATC 1 cut(s) 719
NheI GCTAGC 1 cut(s) 562
NlaIII CATG 1 cut(s) 395
NlaIV GGNNCC 2 cut(s) 175, 244
NmuCI GTSAC 1 cut(s) 101
PaeR7I CTCGAG 1 cut(s) 557
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 1 cut(s) 638
PflFI GACNNNGTC 1 cut(s) 573
PflMI CCANNNNNTGG 1 cut(s) 380
PinAI ACCGGT 1 cut(s) 290
PkrI GCNGC 2 cut(s) 190, 193
PleI GAGTC 2 cut(s) 296, 564
PpsI GAGTC 2 cut(s) 296, 564
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 2 cut(s) 175, 244
PspPI GGNCC 2 cut(s) 173, 272
PstI CTGCAG 1 cut(s) 193
PstNI CAGNNNCTG 2 cut(s) 128, 197
PsuI RGATCY 1 cut(s) 719
PsyI GACNNNGTC 1 cut(s) 573
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SaqAI TTAA 3 cut(s) 24, 42, 465
SatI GCNGC 2 cut(s) 189, 192
Sau3AI GATC 1 cut(s) 719
Sau96I GGNCC 2 cut(s) 173, 272
SchI GAGTC 2 cut(s) 296, 564
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 1 cut(s) 135
SexAI ACCWGGT 1 cut(s) 346
SfaNI GCATC 2 cut(s) 418, 571
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 1 cut(s) 557
SinI GGWCC 2 cut(s) 173, 272
SlaI CTCGAG 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 6 cut(s) 214, 232, 279, 403, 452, 616
SsiI CCGC 4 cut(s) 171, 424, 459, 487
SspMI CTAG 3 cut(s) 147, 563, 567
StyD4I CCNGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 37, 695
TaiI ACGT 1 cut(s) 442
TaqI TCGA 4 cut(s) 236, 558, 606, 636
TasI AATT 6 cut(s) 214, 232, 279, 403, 452, 616
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 3 cut(s) 24, 42, 465
Tru9I TTAA 3 cut(s) 24, 42, 465
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 2 cut(s) 188, 191
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 3 cut(s) 198, 380, 483
TspRI CASTG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 573
Van91I CCANNNNNTGG 1 cut(s) 380
VpaK11BI GGWCC 2 cut(s) 173, 272
XapI RAATTY 2 cut(s) 232, 452
XhoI CTCGAG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 528
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 3 cut(s) 147, 563, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.